Applications of restriction endonuclease fingerprinting of chromosomal DNA of Neisseria meningitidis.

abstract：:A reverse transcription-PCR (RT-PCR) assay based on automated fluorescent capillary electrophoresis and GeneScan software analysis was developed to detect six common respiratory viruses in clinical specimens from young children. Assays for human respiratory syncytial virus (HRSV); human parainfluenza viruses 1, 2, and...

journal_title：Journal of clinical microbiology

authors： Erdman DD,Weinberg GA,Edwards KM,Walker FJ,Anderson BC,Winter J,González M,Anderson LJ

更新日期：2003-09-01 00:00:00

abstract：:Human papillomavirus type 16 (HPV 16) plays a cardinal role in the pathogenesis of cervical cancer. HPV 16 has intratypic variants which show different geographical distributions and different oncogenic potentials. To analyze the presence of sequence variations of HPV 16 variants in northeast China, 71 cervical carcin...

journal_title：Journal of clinical microbiology

authors： Shang Q,Wang Y,Fang Y,Wei L,Chen S,Sun Y,Li B,Zhang F,Gu H

更新日期：2011-07-01 00:00:00

abstract：:The performance of CHROMagar STEC and CHROMagar STEC O104 (CHROMagar Microbiology, Paris, France) media for the detection of Shiga toxin-producing Escherichia coli (STEC) was assessed with 329 stool specimens collected over 14 months from patients with suspected STEC infections (June 2011 to August 2012). The CHROMaga...

journal_title：Journal of clinical microbiology

authors： Gouali M,Ruckly C,Carle I,Lejay-Collin M,Weill FX

更新日期：2013-03-01 00:00:00

abstract：:To detect Streptococcus pneumoniae colonization, the nasopharynx is sampled using a swab placed in skim milk-tryptone-glucose-glycerol (STGG) transport medium, and then the swab specimen or STGG medium is cultured or subjected to PCR. We evaluated the effect of swab composition and compared the sensitivities of detect...

journal_title：Journal of clinical microbiology

authors： Rubin LG,Rizvi A,Baer A

更新日期：2008-08-01 00:00:00

abstract：:The Centers for Disease Control and Prevention recommended confirming positive screening tests for Chlamydia trachomatis when positive predictive values are <90%. It is accepted that less sensitive tests (i.e., culture and immunoassays) should not be used to confirm the results of more sensitive nucleic acid amplifica...

journal_title：Journal of clinical microbiology

authors： Schachter J,Hook EW,Martin DH,Willis D,Fine P,Fuller D,Jordan J,Janda WM,Chernesky M

更新日期：2005-03-01 00:00:00

abstract：:Herpes simplex virus (HSV) infections are very common in the general population and among immunocompromised patients. Acyclovir (ACV) is an effective treatment which is widely used. We deemed it essential to conduct a wide and coordinated survey of the emergence of ACV-resistant HSV strains. We have formed a network o...

journal_title：Journal of clinical microbiology

authors： Danve-Szatanek C,Aymard M,Thouvenot D,Morfin F,Agius G,Bertin I,Billaudel S,Chanzy B,Coste-Burel M,Finkielsztejn L,Fleury H,Hadou T,Henquell C,Lafeuille H,Lafon ME,Le Faou A,Legrand MC,Maille L,Mengelle C,Morand P,

更新日期：2004-01-01 00:00:00

abstract：:A total of 322 single and paired serum samples from children (newborn to 10 years old), young adults (18 to 20 years old), pregnant women and their cord serum samples, and elderly people (more than 70 years old) was tested for antibodies to enteric adenovirus types 40 and 41 by neutralization test. Serum samples were ...

journal_title：Journal of clinical microbiology

authors： Shinozaki T,Araki K,Ushijima H,Fujii R

更新日期：1987-09-01 00:00:00

abstract：:The Leifson staining method was used to diagnose Helicobacter pylori infection and was compared to histology, culture, and the rapid urease test (RUT). Histology gave the best sensitivity (98%), compared to Leifson staining (97%), culture (92%), and RUT (85%) (P < 0.005). Leifson staining is a sensitive, rapid, econom...

journal_title：Journal of clinical microbiology

authors： Piccolomini R,Di Bonaventura G,Neri M,Di Girolamo A,Catamo G,Pizzigallo E

更新日期：1999-01-01 00:00:00

abstract：:As part of an ongoing investigation into nosocomial Legionella infections at Stanford University Medical Center (SUMC), we applied the technique of restriction endonuclease analysis (REA) to determine strain differences among three species, including Legionella pneumophila, Legionella dumoffii, and Legionella micdadei...

journal_title：Journal of clinical microbiology

authors： Tompkins LS,Troup NJ,Woods T,Bibb W,McKinney RM

更新日期：1987-10-01 00:00:00

abstract：:A total of 141 independent strains of Pseudomonas aeruginosa with different heterogeneities in the exo gene (exoS, exoT, exoU, and exoY) background were examined for their pathogenic roles. Results indicated that the exoU gene is the major contributor to cytotoxicity in Madin-Darby canine kidney cells but is not relat...

journal_title：Journal of clinical microbiology

authors： Lin HH,Huang SP,Teng HC,Ji DD,Chen YS,Chen YL

更新日期：2006-12-01 00:00:00

abstract：:Using data from an ongoing Swedish intervention project, the observed durations of nasopharyngeal carriage of penicillin-nonsusceptible Streptococcus pneumoniae (PNSP) (MIC of penicillin G of >or=0.5 microg/ml) stratified by both pneumococcal serogroup and age of the carrier were compared. The means and 95% confidence...

journal_title：Journal of clinical microbiology

authors： Högberg L,Geli P,Ringberg H,Melander E,Lipsitch M,Ekdahl K

更新日期：2007-03-01 00:00:00

abstract：:Moxalactam is highly hydrolyzed by plasmid-mediated metallo-β-lactamases (MBLs), whereas it is poorly inactivated by serine-active carbapenemases. This study demonstrated that moxalactam resistance constituted an effective screen for MBL expression in enterobacteria, which could be confirmed, even in low-MBL-producing...

journal_title：Journal of clinical microbiology

authors： Pluquet E,Arlet G,Bingen E,Drieux L,Mammeri H

更新日期：2011-07-01 00:00:00

abstract：:A disk method for detecting hippurate hydrolysis by Campylobacter spp. was evaluated and compared with the conventional tube test used at the Centers for Disease Control (CDC) (G.K. Morris and C.M. Patton, p. 302-308, in E.H. Lennette, A. Balows, W.J. Hausler, Jr., and H.J. Shadomy, ed., Manual of Clinical Microbiolog...

journal_title：Journal of clinical microbiology

authors： Cacho JB,Aguirre PM,Hernanz A,Velasco AC

更新日期：1989-02-01 00:00:00

abstract：:Antigenic sites on the nucleocapsid (N) protein of severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) were mapped by Pepscan analysis with overlapping peptides that span the N protein sequence. Two major immunodominant epitopes located in the C-terminal region (amino acids [aa] 362 to 412) and middle regi...

journal_title：Journal of clinical microbiology

authors： He Y,Zhou Y,Wu H,Kou Z,Liu S,Jiang S

更新日期：2004-11-01 00:00:00

abstract：:Rickettsiae are gram-negative, obligate intracellular bacteria which have historically been divided into three groups: the typhus group, the scrub typhus group, and the spotted fever group (SFG). Recently, several new SFG rickettsiae have been characterized, and most of these species are associated with ticks and have...

journal_title：Journal of clinical microbiology

authors： Xu W,Raoult D

更新日期：1997-07-01 00:00:00

abstract：:Multiple-locus variable-number tandem-repeat analysis (MLVA) was performed with 292 methicillin-resistant Staphylococcus aureus (MRSA) isolates previously characterized by pulsed-field gel electrophoresis, multilocus sequence typing, and staphylococcal cassette chromosome mec typing. Quantitative correspondence analys...

journal_title：Journal of clinical microbiology

authors： Rivero-Pérez B,Pérez-Roth E,Méndez-Alvarez S

更新日期：2010-08-01 00:00:00

abstract：:We have conducted an international quality assurance study of filovirus, Lassa virus, and orthopox virus PCR with 24 participants. Of the participating laboratories, 45.8 and 66.7% detected virus in all plasma samples, which contained > or = 5,000 and > or = 100,000 copies per ml, respectively. Sensitivity levels were...

journal_title：Journal of clinical microbiology

authors： Niedrig M,Schmitz H,Becker S,Günther S,ter Meulen J,Meyer H,Ellerbrok H,Nitsche A,Gelderblom HR,Drosten C

更新日期：2004-04-01 00:00:00

abstract：:Because strains of Staphylococcus aureus that are resistant to penicillinase-resistant penicillins may be difficult to detect in the clinical laboratory, a variety of changes in methodology have been suggested to increase their detection. In 1984, the West Los Angeles Veterans Administration Medical Center experienced...

journal_title：Journal of clinical microbiology

authors： Mulligan ME,Citron DM,Kwok RY,Wheelock JP,Farrohi FK,Hindler JA,Johnston L

更新日期：1987-05-01 00:00:00

abstract：:The Binax NOW assay (Binax, Inc., Portland, Maine) and the BD Directigen EZ assay (Becton Dickinson and Company, Sparks, Md.), two new rapid immunoassays for detection of respiratory syncytial virus (RSV), as well as the BD Directigen RSV assay (DRSV) (Becton Dickinson and Company) and direct immunofluorescence staini...

journal_title：Journal of clinical microbiology

authors： Ohm-Smith MJ,Nassos PS,Haller BL

更新日期：2004-07-01 00:00:00

abstract：:Broad-range amplification of bacterial DNA from clinical specimens has proved useful for the diagnosis of various bacterial infections, especially during antimicrobial treatment of the patient. Optimal sample processing protocols for diagnostic broad-range bacterial PCR should release DNA from an array of target organ...

journal_title：Journal of clinical microbiology

authors： Rantakokko-Jalava K,Jalava J

更新日期：2002-11-01 00:00:00

abstract：:Phialemonium obovatum was found to be the cause of osteomyelitis in a German shepherd dog. Histologic examination of the biopsied material from the left tibia revealed septate, irregularly branched hyphae, swollen cells, and ovate-to-spherical cells divided by a transverse septum. The majority of the fungal elements w...

journal_title：Journal of clinical microbiology

authors： Lomax LG,Cole JR,Padhye AA,Ajello L,Chandler FW,Smith BR

更新日期：1986-05-01 00:00:00

abstract：:Human adenovirus type 4 is one of the major serotypes isolated from patients with adenoviral conjunctivitis. In 2001 we encountered nosocomial infections with epidemic conjunctivitis in the ophthalmology ward of one hospital in Sapporo, which is in the northern part of Japan. Adenoviruses were isolated from the patien...

journal_title：Journal of clinical microbiology

authors： Ariga T,Shimada Y,Ohgami K,Tagawa Y,Ishiko H,Aoki K,Ohno S

更新日期：2004-08-01 00:00:00

abstract：:Current methods of DNA extraction from different fungal pathogens are often time-consuming and require the use of toxic chemicals. DNA isolation from some fungal organisms is difficult due to cell walls or capsules that are not readily susceptible to lysis. We therefore investigated a new and rapid DNA isolation metho...

journal_title：Journal of clinical microbiology

authors： Müller FM,Werner KE,Kasai M,Francesconi A,Chanock SJ,Walsh TJ

更新日期：1998-06-01 00:00:00

abstract：:We have developed a novel and practical DNA-RNA hybridization assay for the detection and identification of Campylobacter pylori in the gastric mucosa. This technique utilizes a [32P]ddATP-labeled synthetic oligonucleotide probe complementary to a nucleotide sequence present in C. pylori 16S rRNA. This probe is very s...

journal_title：Journal of clinical microbiology

authors： Morotomi M,Hoshina S,Green P,Neu HC,LoGerfo P,Watanabe I,Mutai M,Weinstein IB

更新日期：1989-12-01 00:00:00

abstract：:We evaluated the performance characteristics of a new, real-time nucleic acid sequence-based amplification (NASBA) assay that incorporates molecular beacon technology for detection of human immunodeficiency virus type 1 (HIV-1). The quantitative results were comparable to those obtained with three leading commercially...

journal_title：Journal of clinical microbiology

authors： McClernon DR,Vavro C,St Clair M

更新日期：2006-06-01 00:00:00

abstract：:Mycobacterium avium subsp. paratuberculosis is the causative agent of Johne's disease (JD) in cattle and may be associated with Crohn's disease (CD) in humans. It is the slowest growing of the cultivable mycobacteria, and culture from clinical, veterinary, food, or environmental specimens can take 4 months or even lon...

journal_title：Journal of clinical microbiology

authors： Imirzalioglu C,Dahmen H,Hain T,Billion A,Kuenne C,Chakraborty T,Domann E

更新日期：2011-05-01 00:00:00

abstract：:A commercially available latex agglutination reagent, Directigen (Hynson, Westcott & Dunning, Div. Becton Dickinson & Co., Baltimore, Md.), in which a murine monoclonal antibody to group B streptococcal (GBS) antigen is the active component, was evaluated by using body fluid specimens from 94 sick infants. Antigen was...

journal_title：Journal of clinical microbiology

authors： Rench MA,Metzger TG,Baker CJ

更新日期：1984-11-01 00:00:00

abstract：:Cell line 293, a continuous line of transformed human embryonic kidney cells, has been recognized for its sensitivity in the isolation of adenoviruses, particularly the fastidious species 40 and 41, from stool specimens. To explore the possibility of using this cell line for the isolation of other viruses from clinica...

journal_title：Journal of clinical microbiology

authors： Brown M,Petric M

更新日期：1986-04-01 00:00:00

abstract：:Salmonella typhi was isolated from 369 and Salmonella paratyphi A was isolated from 6 of 515 Vietnamese patients with suspected enteric fever. Compared with conventional broth culture of blood, direct plating of the buffy coat had a diagnostic sensitivity of 99.5% (95% confidence interval [CI], 97.1 to 100%). Blood ba...

journal_title：Journal of clinical microbiology

authors： Wain J,Diep TS,Ho VA,Walsh AM,Nguyen TT,Parry CM,White NJ

更新日期：1998-06-01 00:00:00

abstract：:Autopsy specimens of lung tissues from 15 patients that contracted legionellosis during the 1976 Philadelphia outbreak of Legionnaires disease were examined for the presence of Legionella organisms and soluble antigens by indirect fluorescent-antibody (IFA) testing and by an enzyme-linked immunosorbent assay (ELISA) w...

journal_title：Journal of clinical microbiology

authors： Brown SL,Bibb WF,McKinney RM

更新日期：1985-01-01 00:00:00