当前位置： SCI文献检索 > JOURNAL OF CLINICAL MICROBIOLOGY期刊下所有文献 > Impact of prolonged incubation on disk diffusion susceptibility test results for Staphylococcus aureus.

Impact of prolonged incubation on disk diffusion susceptibility test results for Staphylococcus aureus.

Abstract：

:Because strains of Staphylococcus aureus that are resistant to penicillinase-resistant penicillins may be difficult to detect in the clinical laboratory, a variety of changes in methodology have been suggested to increase their detection. In 1984, the West Los Angeles Veterans Administration Medical Center experienced an increase in clinically significant strains of oxacillin-resistant S. aureus. To insure that such strains would not be missed by the disk diffusion test methods employed for routine testing, changes in methodology were insituted. These included interpreting zone diameters around oxacillin disks at 48 h of incubation. We collected 139 isolates from patients thought to have oxacillin-resistant S. aureus based on these test results and later retested the isolates using microdilution MIC testing. Only 85 isolates (61%) had microdilution oxacillin MICs of greater than or equal to 8.0 micrograms/ml, whereas 54 (39%) had oxacillin MICs of less than or equal to 2.0 micrograms/ml. A review of medical records revealed that in 1 year there were 98 patients with isolates appearing resistant by disk diffusion but not confirmed by microdilution MICs; many patients were placed in isolation and treated with specific antimicrobial agents. We conclude that incubation of oxacillin disk diffusion tests for longer than 24 h in conjunction with disregard for resistance to other classes of antimicrobial agents may result in an unacceptably high degree of false resistance results. Because the resistance of S. aureus has important therapeutic and infection control implications, it is necessary to recognize problems that may result in ambiguous or inaccurate susceptibility results.

journal_name

J Clin Microbiol

journal_title

Journal of clinical microbiology

authors

Mulligan ME,Citron DM,Kwok RY,Wheelock JP,Farrohi FK,Hindler JA,Johnston L

doi

10.1128/JCM.25.5.840-844.1987

subject

Has Abstract

pub_date

1987-05-01 00:00:00

pages

840-4

issue

5

eissn

0095-1137

issn

1098-660X

journal_volume

25

pub_type

杂志文章

相关文献

JOURNAL OF CLINICAL MICROBIOLOGY文献大全
  • Characterization of Metarhizium viride Mycosis in Veiled Chameleons (Chamaeleo calyptratus), Panther Chameleons (Furcifer pardalis), and Inland Bearded Dragons (Pogona vitticeps).

    abstract：:Metarhizium viride has been associated with fatal systemic mycoses in chameleons, but subsequent data on mycoses caused by this fungus in reptiles are lacking. The aim of this investigation was therefore to obtain information on the presence of M. viride in reptiles kept as pets in captivity and its association with c...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.02206-16

    authors： Schmidt V,Klasen L,Schneider J,Hübel J,Pees M

    更新日期：2017-03-01 00:00:00

  • Cytomegalovirus quantitation by real-time PCR is unaffected by delayed separation of plasma from whole blood.

    abstract：:A previous study suggested that delayed sample preparation can cause a false-positive plasma cytomegalovirus (CMV) PCR. We evaluated 78 samples submitted for CMV testing, and we report that a 24-h delay in plasma separation did not affect viral quantitation by real-time PCR. Laboratories should evaluate specimen handl...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/jcm.42.3.1296-1297.2004

    authors： Nesbitt SE,Cook L,Jerome KR

    更新日期：2004-03-01 00:00:00

  • Evaluation of serological tests for diagnosis of Brucella melitensis infection of goats.

    abstract：:Five serological assays were evaluated for the diagnosis of brucellosis in goats: the rose bengal test (RBT), complement fixation test (CFT), radial immunodiffusion (RID) with Brucella and Yersinia enterocolitica O:9 polysaccharides, counterimmunoelectrophoresis (CIEP) with cytosol, and enzyme-linked immunosorbent ass...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.32.5.1159-1165.1994

    authors： Díaz-Aparicio E,Marín C,Alonso-Urmeneta B,Aragón V,Pérez-Ortiz S,Pardo M,Blasco JM,Díaz R,Moriyón I

    更新日期：1994-05-01 00:00:00

  • Amplified fragment length polymorphism reveals specific epigenetic distinctions between Mycobacterium avium subspecies paratuberculosis isolates of various isolation types.

    abstract：:Amplified fragment length polymorphism (AFLP) was employed as a genetic analysis tool for the study of the genetic relatedness of Mycobacterium avium subsp. paratuberculosis isolates harvested from bovine fecal samples and from bovine or human tissues. This analysis revealed genetic differences between these two isola...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.01123-10

    authors： O'Shea B,Khare S,Klein P,Roussel A,Adams LG,Ficht TA,Rice-Ficht AC

    更新日期：2011-06-01 00:00:00

  • Serratia rubidaea as an invasive pathogen.

    abstract：:Serratia rubidaea biotype 1 was isolated from the bile and blood of a patient with a bile tract carcinoma obstructing the common bile duct and who underwent invasive procedures. The infection was cleared after adequate treatment with antibiotics. ...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.34.1.216-217.1996

    authors： Ursua PR,Unzaga MJ,Melero P,Iturburu I,Ezpeleta C,Cisterna R

    更新日期：1996-01-01 00:00:00

  • Disease severity associated with presence in subgingival plaque of Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, and Tannerella forsythia, singly or in combination, as detected by nested multiplex PCR.

    abstract：:This study used a nested multiplex PCR method to detect three periodontal pathogens in subgingival plaque collected before treatment and at 2 and 6 months posttreatment from 107 patients with severe, generalized periodontitis. The proportions of the patients who harbored these bacteria before periodontal treatment wer...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.01007-08

    authors： Ready D,D'Aiuto F,Spratt DA,Suvan J,Tonetti MS,Wilson M

    更新日期：2008-10-01 00:00:00

  • Outbreak of OXY-2-Producing Klebsiella oxytoca in a renal transplant unit.

    abstract：:We describe a Klebsiella oxytoca infection outbreak in a renal transplant unit that involved seven patients. All strains belonged to a single pulsed-field gel electrophoresis pattern and were resistant to amoxicillin-clavulanate, cefuroxime, piperacillin-tazobactam, and aztreonam but susceptible to ceftriaxone, ceftaz...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.00194-08

    authors： Zárate MS,Gales AC,Picão RC,Pujol GS,Lanza A,Smayevsky J

    更新日期：2008-06-01 00:00:00

  • Microbiological Characteristics of Chlamydia trachomatis and Neisseria gonorrhoeae Infections in South African Women.

    abstract：:We analyzed data of 263 women with at least one genital or anorectal sexually transmitted infection from a cross-sectional study conducted in rural South Africa. We provide new insights concerning the concurrence of Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, and Trichomonas vaginalis infectio...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.02848-15

    authors： Dubbink JH,de Waaij DJ,Bos M,van der Eem L,Bébéar C,Mbambazela N,Ouburg S,Peters RP,Morré SA

    更新日期：2016-01-01 00:00:00

  • Diagnosis of Pneumocystis jirovecii pneumonia in immunocompromised patients by real-time PCR: a 4-year prospective study.

    abstract：:Pneumocystis jirovecii pneumonia (PCP) is a life-threatening infection in immunocompromised patients. Quantitative real-time PCR (qPCR) is more sensitive than microscopic examination for the detection of P. jirovecii but also detects colonized patients. Hence, its positive predictive value (PPV) needs evaluation. In t...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.01480-14

    authors： Robert-Gangneux F,Belaz S,Revest M,Tattevin P,Jouneau S,Decaux O,Chevrier S,Le Tulzo Y,Gangneux JP

    更新日期：2014-09-01 00:00:00

  • Cross-reactions between pneumococci and other streptococci due to C polysaccharide and F antigen.

    abstract：:By serological methods, all 83 known types of Streptococcus pneumoniae could be shown to possess C polysaccharide and F antigen. Cross-reactions due to these two antigens between pneumococci and a broad range of most other commonly encountered streptococci were examined. The presence of an antigen closely similar or i...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.25.10.1854-1859.1987

    authors： Sørensen UB,Henrichsen J

    更新日期：1987-10-01 00:00:00

  • Aspergillus citrinoterreus, a new species of section Terrei isolated from samples of patients with nonhematological predisposing conditions.

    abstract：:The use of molecular identification techniques has revealed an increasing number of new species within Aspergillus section Terrei. We phenotyped a set of 26 clinical isolates that showed genetic differences from Aspergillus terreus sensu stricto by analyzing sequences from PCR-amplified β-tubulin and calmodulin genes ...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.03088-14

    authors： Guinea J,Sandoval-Denis M,Escribano P,Peláez T,Guarro J,Bouza E

    更新日期：2015-02-01 00:00:00

  • Species-specific detection of Legionella pneumophila in water by DNA amplification and hybridization.

    abstract：:A sensitive detection system specific for Legionella pneumophila in water was developed. This system is based on amplification of a chromosomal DNA sequence from L. pneumophila by the polymerase chain reaction, followed by detection of the amplified product by hybridization of a radiolabeled oligodeoxynucleotide. Afte...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.27.6.1257-1261.1989

    authors： Starnbach MN,Falkow S,Tompkins LS

    更新日期：1989-06-01 00:00:00

  • Direct comparison of the BACTEC 9240 and BacT/ALERT 3D automated blood culture systems for candida growth detection.

    abstract：:A direct comparison of two automated blood culture systems was conducted to compare their ability to detect Candida growth. The systems evaluated were the BACTEC 9240 (Bactec) and BacT/ALERT 3D (BacT). The aerobic, anaerobic, and mycology media for each system were evaluated: Bactec Plus Aerobic/F, Plus Anaerobic/F, a...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/jcm.42.1.115-118.2004

    authors： Horvath LL,George BJ,Murray CK,Harrison LS,Hospenthal DR

    更新日期：2004-01-01 00:00:00

  • Discordance between mycobacterial interspersed repetitive-unit-variable-number tandem-repeat typing and IS6110 restriction fragment length polymorphism genotyping for analysis of Mycobacterium tuberculosis Beijing strains in a setting of high incidence of

    abstract：:IS6110 restriction fragment length polymorphism (RFLP) genotyping is the most widely used genotyping method to study the epidemiology of Mycobacterium tuberculosis. However, due to the complexity of the IS6110 RFLP genotyping technique, and the interpretation of RFLP data, mycobacterial interspersed repetitive-unit-va...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.00770-08

    authors： Hanekom M,van der Spuy GD,Gey van Pittius NC,McEvoy CR,Hoek KG,Ndabambi SL,Jordaan AM,Victor TC,van Helden PD,Warren RM

    更新日期：2008-10-01 00:00:00

  • Definitive differentiation between single and mixed mycobacterial infections in red deer (Cervus elaphus) by a combination of duplex amplification of p34 and f57 sequences and Hpy188I enzymatic restriction of duplex amplicons.

    abstract：:Severe emaciation and mortalities suggestive of mycobacterial infections were recently reported for both adult and young wild red deer (Cervus elaphus) in the southeastern part of Belgium. In deer, tuberculous lesions are not pathognomonic of Mycobacterium bovis infection due to gross and microscopic similarities with...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.43.9.4640-4648.2005

    authors： Godfroid J,Delcorps C,Irenge LM,Walravens K,Marché S,Gala JL

    更新日期：2005-09-01 00:00:00

  • Name Changes for Fungi of Medical Importance, 2018 to 2019.

    abstract：:The current article summarizes recent changes in nomenclature for fungi of medical importance published in the years 2018 to 2019, including new species and revised names for existing ones. Many of the revised names have been widely adopted without further discussion. However, those that concern common pathogens of hu...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章,评审

    doi：10.1128/JCM.01811-20

    authors： Borman AM,Johnson EM

    更新日期：2021-01-21 00:00:00

  • Molecular epidemiology of human rotaviruses in Melbourne, Australia, from 1973 to 1979, as determined by electrophoresis of genome ribonucleic acid.

    abstract：:Rotaviruses contain a double-stranded ribonucleic acid genome consisting of 11 segments. Gel electrophoresis separates genome segments and allows identification of strain differences. This electrophoretic typing technique was applied to rotavirus specimens from 116 children and 72 newborn babies. Between 1973 and 1979...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.13.2.272-278.1981

    authors： Rodger SM,Bishop RF,Birch C,McLean B,Holmes IH

    更新日期：1981-02-01 00:00:00

  • Rapid, simple method of preparing rotaviral double-stranded ribonucleic acid for analysis by polyacrylamide gel electrophoresis.

    abstract：:A procedure for extracting rotaviral double-stranded ribonucleic acid (RNA) directly from fecal and intestinal specimens collected from calves and pigs is described. This procedure provides a rapid, simple, reproducible method of obtaining rotaviral double-stranded RNA preparations suitable for electrophoretic analysi...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.14.3.273-280.1981

    authors： Theil KW,McCloskey CM,Saif LJ,Redman DR,Bohl EH,Hancock DD,Kohler EM,Moorhead PD

    更新日期：1981-09-01 00:00:00

  • Evaluation of new colorimetric vitek 2 yeast identification card by use of different source media.

    abstract：:The new colorimetric Vitek 2 YST card was evaluated for identification of yeasts (136 strains) with respect to the influence of different source media. The Vitek 2 YST card achieved satisfactory results for all yeast species tested, with the exception of Candida guilliermondii, Candida norvegensis, Candida parapsilosi...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.01318-08

    authors： Valenza G,Strasen J,Schäfer F,Frosch M,Kurzai O,Abele-Horn M

    更新日期：2008-11-01 00:00:00

  • Detection of Campylobacter jejuni by culture and real-time PCR in a French cohort of patients with Guillain-Barre syndrome.

    abstract：:Bacteriological culture and real-time PCR (RT-PCR) were used to detect Campylobacter jejuni in fecal samples from a French cohort of 237 patients with Guillain-Barré syndrome (GBS). We provide evidence that diverse serotypes and genotypes of C. jejuni are a major trigger of GBS in France. ...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.00381-10

    authors： Sivadon-Tardy V,Orlikowski D,Porcher R,Ronco E,Caudie C,Roussi J,Fauchère JL,Mégraud F,Tabor H,Sharshar T,Annane D,Raphaël JC,Gaillard JL

    更新日期：2010-06-01 00:00:00

  • Detection of human parvovirus B19 DNA by using the polymerase chain reaction.

    abstract：:The polymerase chain reaction (PCR) was investigated for detecting human parvovirus B19 (B19) DNA in sera. Three pairs of oligonucleotides were evaluated as primers. The best oligonucleotide pair spanned 699 nucleotides, including the region common to VP1 and VP2. After PCR amplification of B19 DNA in serum, a 699-nuc...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.28.1.65-69.1990

    authors： Koch WC,Adler SP

    更新日期：1990-01-01 00:00:00

  • Detection of binding antibodies to native and recombinant human immunodeficiency virus type 1 envelope glycoproteins following recombinant gp160 immunization measured by flow cytometry and enzyme immunoassays. The AIDS Vaccine Clinical Trials Network.

    abstract：:The ability of antibody induced by vaccination with recombinant gp160 (rgp160) to bind to native and recombinant human immunodeficiency virus type 1 (HIV-1) envelope glycoproteins was measured. Thirty-three HIV-1-seronegative healthy adult volunteers were injected four times with 40 or 80 micrograms of an HIV-1LAV env...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.30.10.2606-2612.1992

    authors： Gorse GJ,Frey SE,Newman FK,Belshe RB

    更新日期：1992-10-01 00:00:00

  • Clinical and serological variation between patients infected with different Hepatitis B virus genotypes.

    abstract：:Hepatitis B virus (HBV) has eight genotypes which have distinct geographical distributions. Studies comparing differences in the clinical outcomes of infections caused by strains with genotype-related variations in the HBV genome have largely compared genotypes B and C and genotypes A and D but not all four genotypes....

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.42.12.5837-5841.2004

    authors： Kidd-Ljunggren K,Myhre E,Bläckberg J

    更新日期：2004-12-01 00:00:00

  • Comparison of in vitro activities of 17 antifungal drugs against a panel of 20 dermatophytes by using a microdilution assay.

    abstract：:The in vitro activities of 17 antifungal drugs against a panel of 20 dermatophytes comprising 6 different species were determined using a microdilution assay according to the NCCLS M38-P method with some modifications. Terbinafine was the most potent systemic drug while tolnaftate and amorolfine were the most active t...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/jcm.41.10.4817-4819.2003

    authors： Favre B,Hofbauer B,Hildering KS,Ryder NS

    更新日期：2003-10-01 00:00:00

  • Colorimetric method for rapid determination of bacteriuria.

    abstract：:An inexpensive, rapid, and simple colorimetric test for detection of bacteriuria is described. This test does not require bacterial growth and has the marked advantage of being able to quantify bacteria, even when the organisms are present in the urine of bacteriuric patients who are being treated with antibiotics. Th...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.14.3.342-346.1981

    authors： Wallis C,Melnick JL,Longoria CJ

    更新日期：1981-09-01 00:00:00

  • Fully automated quantification of cytomegalovirus (CMV) in whole blood with the new sensitive Abbott RealTime CMV assay in the era of the CMV international standard.

    abstract：:Fully standardized reproducible and sensitive quantification assays for cytomegalovirus (CMV) are needed to better define thresholds for antiviral therapy initiation and interruption. We evaluated the newly released Abbott RealTime CMV assay for CMV quantification in whole blood (WB) that includes automated extraction...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.00067-13

    authors： Schnepf N,Scieux C,Resche-Riggon M,Feghoul L,Xhaard A,Gallien S,Molina JM,Socié G,Viglietti D,Simon F,Mazeron MC,Legoff J

    更新日期：2013-07-01 00:00:00

  • Usefulness of plasmid profiles for differentiation of Shigella isolates in Bangladesh.

    abstract：:We studied the plasmid profiles of 136 Shigella isolates in Bangladesh to determine whether plasmid profiles could be used for differentiation of strains for epidemiological studies. Many different plasmid patterns were observed within each species, indicating that many genetically different strains of Shigella are re...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.20.2.300-301.1984

    authors： Tacket CO,Shahid N,Huq MI,Alim AR,Cohen ML

    更新日期：1984-08-01 00:00:00

  • Screening for antimicrobial resistance in fecal samples by the replica plating method.

    abstract：:Replica plating can be used for the detection of antibiotic resistance in normal flora. We have evaluated this application of the replica plating method by comparing it with a five-colony method. The replica plating method uses a single plate for each antibiotic, with a concentration just above that for borderline res...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：10.1128/JCM.33.12.3146-3149.1995

    authors： Osterblad M,Leistevuo T,Huovinen P

    更新日期：1995-12-01 00:00:00

  • Most Enterobacter aerogenes strains in France belong to a prevalent clone.

    abstract：:The aim of this study was to determine the distribution in France of the Enterobacter aerogenes prevalent clone isolated in the hospitals of the Marseille area (A. Davin-Regli, D. Monnet, P. Saux, C. Bosi, R. Charrel, A. Barthelemy, and C. Bollet, J. Clin. Microbiol. 34:1474-1480, 1996). A total of 123 E. aerogenes is...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章,多中心研究

    doi：10.1128/JCM.37.7.2165-2169.1999

    authors： Bosi C,Davin-Regli A,Bornet C,Mallea M,Pages JM,Bollet C

    更新日期：1999-07-01 00:00:00

  • Persistence of immunoglobulin G and immunoglobulin M antibodies after postnatal rubella infection determined by solid-phase radioimmunoassay.

    abstract：:The appearance and persistence of immunoglobulin M (IgM) and IgG antibodies in postnatal rubella infections were studied by employing a solid-phase radioimmunoassay test. Altogether, 222 serial serum specimens from 51 patients with acute rubella infection were tested. Both IgG and IgM antibodies developed rapidly and ...

    journal_title：Journal of clinical microbiology

    pub_type： 杂志文章

    doi：

    authors： Meurman OH

    更新日期：1978-01-01 00:00:00