Abstract:
:Amplified fragment length polymorphism (AFLP) was employed as a genetic analysis tool for the study of the genetic relatedness of Mycobacterium avium subsp. paratuberculosis isolates harvested from bovine fecal samples and from bovine or human tissues. This analysis revealed genetic differences between these two isolate types that were confirmed through cluster analysis. Dendrogram analysis separated these two isolate types based on the isolation scheme (tissue-associated versus fecal M. avium subsp. paratuberculosis isolates). Further sequence analysis of unique genetic regions from each isolation type revealed no genetic sequence differences. However, Clustal DNA alignments identified AFLP restriction enzyme sites that were undigested in the tissue-associated isolates. AFLP analysis also disclosed that the same AFLP restriction sites were digested in all of the fecal isolates. Sequence analysis further revealed a consensus sequence upstream of the undigested restriction sites for possible methyltransferase recognition in the tissue-associated M. avium subsp. paratuberculosis isolates.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
O'Shea B,Khare S,Klein P,Roussel A,Adams LG,Ficht TA,Rice-Ficht ACdoi
10.1128/JCM.01123-10subject
Has Abstractpub_date
2011-06-01 00:00:00pages
2222-9issue
6eissn
0095-1137issn
1098-660Xpii
JCM.01123-10journal_volume
49pub_type
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