Comparison of two rapid commercial tests with complement fixation for serologic diagnosis of Mycoplasma pneumoniae infections.


:The complement fixation (CF) test is the current reference serologic test for the diagnosis of Mycoplasma pneumoniae infection. However, it is reported to be insensitive and nonspecific, and it is labor intensive. To determine if a faster and more sensitive diagnosis of M. pneumoniae could be obtained, we examined 50 paired serum samples from patients with suspected M. pneumoniae infection by the CF test and two commercial rapid antibody detection kits, the Remel M. pneumoniae immunoglobulin G (IgG)-IgM antibody test system (Remel, Lenexa, Kans.) and the Seradyn Color Vue M. pneumoniae IgG-IgM kit (Seradyn, Indianapolis, Ind.). The Remel test, a 5-min qualitative immunobinding assay, detected antibodies in three patient serum samples with CF titers of 32 and in all but one sample with titers of > or = 64. The Seradyn test, a 40-min qualitative agglutination test, was less sensitive than CF or Remel. The Seradyn test was positive in 68% of cases, compared with 94 and 96% of cases tested by CF or Remel, respectively. Both commercial tests are faster and less technically demanding to perform than is the CF test.


J Clin Microbiol


Thacker WL,Talkington DF




Has Abstract


1995-05-01 00:00:00












  • Evaluation of Streck tissue fixative, a nonformalin fixative for preservation of stool samples and subsequent parasitologic examination.

    abstract::We undertook a study to evaluate Streck tissue fixative (STF) as a substitute for formalin and polyvinyl alcohol (PVA) in fecal preservation. A comparison of formalin, PVA, (mercuric chloride based), and STF was done by aliquoting fecal samples into each fixative. Stool specimens were collected in Haiti, and parasites...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Nace EK,Steurer FJ,Eberhard ML

    更新日期:1999-12-01 00:00:00

  • Systematic review and meta-analysis of a urine-based pneumococcal antigen test for diagnosis of community-acquired pneumonia caused by Streptococcus pneumoniae.

    abstract::Standard culture methods for diagnosis of Streptococcus pneumoniae pneumonia take at least 24 h. The BinaxNOW urine-based test for S. pneumoniae (BinaxNOW-SP) takes only 15 min to conduct, potentially enabling earlier diagnosis and targeted treatment. This study was conducted to assess whether the use of BinaxNOW-SP a...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,meta分析,评审


    authors: Sinclair A,Xie X,Teltscher M,Dendukuri N

    更新日期:2013-07-01 00:00:00

  • Cerebrospinal fluid lactic acid in diagnosis of meningitis.

    abstract::Quantitative lactate determinations were performed on cerebrospinal fluids to assess their value in the rapid diagnosis of bacterial and mycotic meningitis and to evaluate their value in assessing the prognosis in these patients. Cerebrospinal fluid lactate concentrations were elevated in all patients with untreated b...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Komorowski RA,Farmer SG,Hanson GA,Hause LL

    更新日期:1978-07-01 00:00:00

  • Environmental isolation of Balamuthia mandrillaris associated with a case of amebic encephalitis.

    abstract::This report describes the first isolation of the ameba Balamuthia mandrillaris from an environmental soil sample associated with a fatal case of amebic encephalitis in a northern California child. Isolation of the ameba into culture from autopsied brain tissue confirmed the presence of Balamuthia: In trying to locate ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Schuster FL,Dunnebacke TH,Booton GC,Yagi S,Kohlmeier CK,Glaser C,Vugia D,Bakardjiev A,Azimi P,Maddux-Gonzalez M,Martinez AJ,Visvesvara GS

    更新日期:2003-07-01 00:00:00

  • Candida auris Clinical Isolates from South Korea: Identification, Antifungal Susceptibility, and Genotyping.

    abstract::Candida auris is an emerging worldwide fungal pathogen. Over the past 20 years, 61 patient isolates of C. auris (4 blood and 57 ear) have been obtained from 13 hospitals in Korea. Here, we reanalyzed those molecularly identified isolates using two matrix-assisted laser desorption ionization-time of flight mass spectro...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Kwon YJ,Shin JH,Byun SA,Choi MJ,Won EJ,Lee D,Lee SY,Chun S,Lee JH,Choi HJ,Kee SJ,Kim SH,Shin MG

    更新日期:2019-03-28 00:00:00

  • Characterization of Finnish Mycobacterium tuberculosis isolates by spoligotyping.

    abstract::The molecular epidemiology of tuberculosis (TB) in Finland was studied by spoligotyping 380 Mycobacterium tuberculosis isolates. The isolates were obtained during a 1-year study period from July 2000 to June 2001 and represented 90% of new M. tuberculosis findings by culture in the whole country during the study perio...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Puustinen K,Marjamäki M,Rastogi N,Sola C,Filliol I,Ruutu P,Holmström P,Viljanen MK,Soini H

    更新日期:2003-04-01 00:00:00

  • Recovery of Gram-Negative Bacteria from Aerobic Blood Culture Bottles Containing Antibiotic Binding Resins after Exposure to β-Lactam and Fluoroquinolone Concentrations.

    abstract::Blood culture bottles containing antibiotic binding resins are routinely used to minimize artificial sterilization in the presence of antibiotics. However, the resin binding kinetics can differ between antibiotics and concentrations. This study assessed the impact of clinically meaningful peak, midpoint, and trough co...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Chen IH,Nicolau DP,Kuti JL

    更新日期:2019-09-24 00:00:00

  • Development and evaluation of a liquid bead microarray assay for genotyping genital human papillomaviruses.

    abstract::We developed a liquid bead microarray (LBMA) assay for genotyping genital human papillomaviruses (HPVs) based on the MY09-MY11-HMB01 PCR system and the reverse line blot (RLB) assay probe sequences. Using individual HPV plasmids, we were able to detect as few as 50 copies per reaction. In two separate retrospective st...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Feng Q,Cherne S,Winer RL,Balasubramanian A,Lee SK,Hawes SE,Kiviat NB,Koutsky LA

    更新日期:2009-03-01 00:00:00

  • Heterogeneity and compartmentalization of Pneumocystis carinii f. sp. hominis genotypes in autopsy lungs.

    abstract::The extent and importance of genotype heterogeneity of Pneumocystis carinii f. sp. hominis within lungs have not previously been investigated. Two hundred forty PCR clones obtained from respiratory specimens and lung segments from three patients with fatal P. carinii pneumonia were investigated to detect genetic diver...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Helweg-Larsen J,Lundgren B,Lundgren JD

    更新日期:2001-10-01 00:00:00

  • Persistence of antibodies to rotavirus in human milk.

    abstract::Human milk obtained from 21 American nursing mothers was studied for the presence of secretory immunoglobulin A antibody to rotavirus, the most common etiological agent of infantile gastroenteritis. Antibody was quantitated by adaptation of a recently described solid-phase radioimmunoassay technique that employs simia...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Cukor G,Blacklow NR,Capozza FE,Panjvani ZF,Bednarek F

    更新日期:1979-01-01 00:00:00

  • Controlled clinical laboratory comparison of BACTEC plus aerobic/F resin medium with BacT/Alert aerobic FAN medium for detection of bacteremia and fungemia.

    abstract::Blood specimens collected from adult patients with suspected sepsis in four medical centers were inoculated into BACTEC Plus/F and BacT/Alert FAN aerobic culture bottles. Both bottles of 7,401 bottle pairs contained the prescribed blood volume of 8 to 12 ml. Bottles were incubated in their respective instruments for a...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Jorgensen JH,Mirrett S,McDonald LC,Murray PR,Weinstein MP,Fune J,Trippy CW,Masterson M,Reller LB

    更新日期:1997-01-01 00:00:00

  • DNA homology and immunological cross-reactivity between Aeromonas hydrophila cytotonic toxin and cholera toxin.

    abstract::DNA colony hybridization with three 18- to 20-base-long synthetic oligonucleotide probes for cholera toxin (CT) was used to screen 12 clinical isolates of Aeromonas hydrophila. Under stringent hybridizing (overnight at 40 degrees C) and washing (1 h at 50 degrees C) conditions, nine strains reacted with the 32P-labele...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Schultz AJ,McCardell BA

    更新日期:1988-01-01 00:00:00

  • Rapid and simultaneous detection of genes encoding Klebsiella pneumoniae carbapenemase (blaKPC) and New Delhi metallo-β-lactamase (blaNDM) in Gram-negative bacilli.

    abstract::We present a duplex, real-time PCR assay for detection of Klebsiella pneumoniae carbapenemase (blaKPC) and New Delhi metallo-β-lactamase (blaNDM) genes. Accuracy was assessed with 158 Gram-negative bacillary isolates, including 134 carbapenemase producers. The assay had 100% sensitivity and specificity compared with r...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Cunningham SA,Noorie T,Meunier D,Woodford N,Patel R

    更新日期:2013-04-01 00:00:00

  • Optimal DNA isolation method for detection of bacteria in clinical specimens by broad-range PCR.

    abstract::Broad-range amplification of bacterial DNA from clinical specimens has proved useful for the diagnosis of various bacterial infections, especially during antimicrobial treatment of the patient. Optimal sample processing protocols for diagnostic broad-range bacterial PCR should release DNA from an array of target organ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Rantakokko-Jalava K,Jalava J

    更新日期:2002-11-01 00:00:00

  • Comparison of the semisolid agar antifungal susceptibility test with the NCCLS M38-P broth microdilution test for screening of filamentous fungi.

    abstract::Antifungal susceptibility testing of pathogenic molds is being developed. A simple screening semisolid agar antifungal susceptibility (SAAS) test accurately measures susceptibilities of yeasts. The performance of the SAAS screening test for filamentous fungi was assessed by comparing MICs of four antifungals (amphoter...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Kuzucu C,Rapino B,McDermott L,Hadley S

    更新日期:2004-03-01 00:00:00

  • Development of revised quality control limits for disk diffusion susceptibility tests of selected cephem antibiotics with Haemophilus influenzae and description of a new control strain.

    abstract::Inconsistent quality control results in disk diffusion testing of cefaclor, cefamandole, cefonicid, and cefuroxime with Haemophilus influenzae ATCC 49247 and Haemophilus test medium (HTM) prompted a search for an alternative control strain that would provide more reliable results. A five-laboratory study was conducted...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Jorgensen JH,Barry AL,Doern GV,Ferraro MJ,Murray PR

    更新日期:1992-08-01 00:00:00

  • Comparison of Gen-Probe DNA probe test and culture for the detection of Neisseria gonorrhoeae in endocervical specimens.

    abstract::A 2-h nonisotopic DNA probe assay for the direct detection of Neisseria gonorrhoeae in urogenital specimens has recently been modified (PACE 2; Gen-Probe, San Diego, Calif.). The new assay format was developed to increase the sensitivity of the assay and simplify procedural steps. In this study, the new DNA probe test...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Panke ES,Yang LI,Leist PA,Magevney P,Fry RJ,Lee RF

    更新日期:1991-05-01 00:00:00

  • Identification of Acinetobacter baumannii by detection of the blaOXA-51-like carbapenemase gene intrinsic to this species.

    abstract::bla(OXA-51-like) was sought in clinical isolates of Acinetobacter species in a multiplex PCR, which also detects bla(OXA-23-like) and class 1 integrase genes. All isolates that gave a band for bla(OXA-51-like) identified as A. baumannii. This gene was detected in each of 141 isolates of A. baumannii but not in those o...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Turton JF,Woodford N,Glover J,Yarde S,Kaufmann ME,Pitt TL

    更新日期:2006-08-01 00:00:00

  • Clinical laboratory evaluation of automated microbial detection/identification system in analysis of clinical urine specimens.

    abstract::More than 4,000 clinical urine specimens were evaluated with an automated microbial detection/identification system compared to a standarized manual analysis and the routine modalities used in five peer-group laboratories. The comparison indicates that the automated system recognizes the nine groups of significant mic...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Isenberg HD,Gavan TL,Sonnenwirth A,Taylor WI,Washington JA 2nd

    更新日期:1979-08-01 00:00:00

  • Comparison of four charcoal media for the isolation of Bordetella pertussis.

    abstract::Charcoal-horse blood agar with 40 micrograms of cephalexin per ml, charcoal-horse blood agar with 3 micrograms of lincomycin per ml, charcoal agar with 3 micrograms of lincomycin per ml, and Legionella (buffered charcoal-yeast extract) agar with 3 micrograms of lincomycin per ml were compared for isolation of Bordetel...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Hoppe JE,Schwaderer J

    更新日期:1989-05-01 00:00:00

  • Variability in CO2, O2, and pH levels in blood culture bottles from five different manufacturers.

    abstract::The CO2, O2, and pH levels of commercially available blood culture bottles with tryptic soy broth medium from five different manufacturers were compared. Ranges of 1.3 to 6.9% for CO2, 1.1 to 6.0% for O2, and pH 6.94 to 7.26 were found. Different venting procedures revealed that blood culture bottles from which the ru...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Welch WD,Porschen RK,Zarifi SZ

    更新日期:1984-11-01 00:00:00

  • Growth of Paracoccidioides brasiliensis yeast phase in a chemically defined culture medium.

    abstract::A slight modification of the chemically defined medium of McVeigh and Morton resulted in an excellent substratum for the cultivation of Paracoccidioides brasiliensis yeast phase. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Restrepo A,Jiménez BE

    更新日期:1980-08-01 00:00:00

  • Isolation of Salmonella enteritidis, serotype Horsham, from three American Indian Tribes.

    abstract::Salmonella enteritidis, serotype Horsham, identified rarely worldwide, was isolated from the stools of six American Indians with gastroenteritis. The patients were from separate tribes located in three geographically distinct villages. Although significant contact was demonstrated between ill individuals within two tr...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Castle SP,Nims LJ,Lapham SC

    更新日期:1983-07-01 00:00:00

  • Rapid detection of Mycobacterium tuberculosis in contaminated BACTEC 12B broth cultures by testing with Amplified Mycobacterium Tuberculosis Direct Test.

    abstract::Contamination of broth cultures of acid-fast bacilli (AFB) by bacterial species other than Mycobacterium species frequently occurs. Many of these contaminated cultures require redecontamination and reincubation before the appropriate tests can be performed for identification, significantly affecting the turnaround tim...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Zheng X,Pang M,Engler HD,Tanaka S,Reppun T

    更新日期:2001-10-01 00:00:00

  • Detection of Helicobacter pylori in fecal samples of gnotobiotic mice infected with H. pylori by an immunomagnetic-bead separation technique.

    abstract::By an immunomagnetic-bead (IMB) separation technique, isolation of Helicobacter pylori from gastrointestinal and fecal samples of gnotobiotic mice infected with the microorganism was tried. The isolation rate of H. pylori from stomach samples after IMB separation was not higher than that of direct culture of the sampl...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Osaki T,Taguchi H,Yamaguchi H,Kamiya S

    更新日期:1998-01-01 00:00:00

  • Improved version of the exoantigen test for identification of Coccidioides immitis and Histoplasma capsulatum cultures.

    abstract::A rapid and simple method for extracting specific cell-free antigens of Coccidioides immitis and Histoplasma capsulatum from agar slant cultures was developed. The extracts were analyzed in immunodiffusion tests for the presence of C. immitis or H. capsulatum specific antigens. These extracts were compared with cultur...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Kaufman L,Standard P

    更新日期:1978-07-01 00:00:00

  • Cytopathic astrovirus isolated from porcine acute gastroenteritis in an established cell line derived from porcine embryonic kidney.

    abstract::A cytopathic astrovirus was isolated from pigs with acute diarrhea in an established cell line that was derived from porcine embryonic kidneys with the aid of trypsin. The virus showed a distinct cytopathic effect characterized by an enlargement of cells and the appearance of fine granules in the cytoplasm. Porcine as...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Shimizu M,Shirai J,Narita M,Yamane T

    更新日期:1990-02-01 00:00:00

  • Diaper area skin microflora of normal children and children with atopic dermatitis.

    abstract::In vitro studies established that neither cloth nor disposable diapers demonstrably contributed to the growth of Escherichia coli, Proteus vulgaris, Staphylococcus aureus, or Candida albicans when urine was present as a growth medium. In a clinical study of 166 children, the microbial skin flora of children with atopi...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Keswick BH,Seymour JL,Milligan MC

    更新日期:1987-02-01 00:00:00

  • Simultaneous runs of the Bayer VERSANT HIV-1 version 3.0 and HCV bDNA version 3.0 quantitative assays on the system 340 platform provide reliable quantitation and improved work flow.

    abstract::Branched DNA (bDNA) assays to quantify human immunodeficiency virus type 1 (HIV-1) and hepatitis C virus (HCV) consist of three distinct steps, including sample processing, hybridization, and detection, and utilize the System 340 platform for plate incubation and washing. Sample processing differs: HIV-1 from 1 ml of ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Elbeik T,Markowitz N,Nassos P,Kumar U,Beringer S,Haller B,Ng V

    更新日期:2004-07-01 00:00:00

  • Disseminated Scedosporium/Pseudallescheria infection after double-lung transplantation in patients with cystic fibrosis.

    abstract::We report a case of disseminated Scedosporium/Pseudallescheria infection due to Pseudallescheria boydii sensu stricto after lung transplantation in a patient with cystic fibrosis. Dissemination occurred under voriconazole. Despite surgery and combination therapy with voriconazole, caspofungin, and terbinafine, the pat...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Morio F,Horeau-Langlard D,Gay-Andrieu F,Talarmin JP,Haloun A,Treilhaud M,Despins P,Jossic F,Nourry L,Danner-Boucher I,Pattier S,Bouchara JP,Le Pape P,Miegeville M

    更新日期:2010-05-01 00:00:00