Comparison of Southern blot hybridization and polymerase chain reaction methods for the detection of human papillomavirus DNA.

abstract：:A serum immunoglobulin G enzyme immunoassay (EIA) for Helicobacter pylori antibodies already in use in adults was evaluated with 99 pediatric serum samples to determine its usefulness for the study of H. pylori disease in children. The reference method used was either the (13)C-urea breath test or a biopsy culture of ...

journal_title：Journal of clinical microbiology

authors： Sunnerstam B,Kjerstadius T,Jansson L,Giesecke J,Bergström M,Ejderhamn J

更新日期：1999-10-01 00:00:00

abstract：:Human papillomavirus (HPV) DNA detection and typing are important for diagnosis and management of HPV-associated diseases. One of the most commonly used PCR methods, GP5+/6+, shows weaknesses in amplifying certain types. To circumvent this limitation, we developed and validated broad-spectrum primers targeting the GP5...

journal_title：Journal of clinical microbiology

authors： Schmitt M,Dondog B,Waterboer T,Pawlita M

更新日期：2008-03-01 00:00:00

abstract：:JC polyomavirus (JCV) is the causative agent of progressive multifocal leukoencephalopathy (PML), a central nervous system infection that mainly affects AIDS patients. The extensive application of highly active antiretroviral therapy (HAART) is leading to the appearance of "long-term" survival PML patients. A reliable...

journal_title：Journal of clinical microbiology

authors： García de Viedma D,Alonso R,Miralles P,Berenguer J,Rodriguez-Créixems M,Bouza E

更新日期：1999-03-01 00:00:00

abstract：:A rapid, sensitive counterimmunoelectrophoresis assay was developed to detect adenovirus in stools of patients with gastroenteritis. The parameters of the assay were optimized, and its sensitivity and efficiency was examined. The assay promises to be a useful alternative to conventional techniques of cell culture isol...

journal_title：Journal of clinical microbiology

authors： Mankikar SD,Middleton PJ,Petric M

更新日期：1979-08-01 00:00:00

abstract：:Fifty-six Pasteurella multocida strains (40 P. multocida subsp. septica and 16 P. multocida subsp. multocida strains) isolated from the mouths of 56 dogs among the 134 living in a French canine military training center (132e Groupe Cynophile de l'Armée de Terre, Suippes, France) were studied by use of enterobacterial ...

journal_title：Journal of clinical microbiology

authors： Loubinoux J,Lozniewski A,Lion C,Garin D,Weber M,Le Faou AE

更新日期：1999-08-01 00:00:00

abstract：:Of the media examined, thioglycolate broth supplemented with 5% lysed sheep blood, Butzler antibiotic mixture, and 0.1% lauryl sulfate was the most sensitive enrichment medium for recovery of wild-type strains of Campylobacter jejuni from cecal contents of chickens and chicken livers. It allowed the retrieval of 1 CFU...

journal_title：Journal of clinical microbiology

authors： Barot MS,Bokkenheuser VD

更新日期：1984-07-01 00:00:00

abstract：:Forested areas adjacent to Milwaukee, Wis., and Chicago, Ill., were investigated for rodents and ticks infected with Borrelia burgdorferi, the causative agent of Lyme disease. White-footed mice (Peromyscus leucopus or Peromyscus maniculatus), meadow voles (Microtus pennsylvanicus), and eastern chipmunks (Tamias striat...

journal_title：Journal of clinical microbiology

authors： Callister SM,Nelson JA,Schell RF,Jobe DA,Bautz R,Agger WA,Coggins J

更新日期：1991-02-01 00:00:00

abstract：:During a 3-year period, all Pasteurella strains recovered at the Clinical Microbiological Laboratory, Lund, Sweden, were studied biochemically with respect to their relationship to the recently described taxa of this genus. Of 159 strains recovered from 146 infected humans, 95 were identified as Pasteurella multocida ...

journal_title：Journal of clinical microbiology

authors： Holst E,Rollof J,Larsson L,Nielsen JP

更新日期：1992-11-01 00:00:00

abstract：:Antibiogram typing of methicillin-resistant Staphylococcus aureus with selected antibiotics was evaluated as a primary epidemiological typing tool and compared with ribotyping. Antibiograms were derived with the Kirby-Bauer disk diffusion method by using erythromycin, clindamycin, cotrimoxazole, gentamicin, and ciprof...

journal_title：Journal of clinical microbiology

authors： Blanc DS,Lugeon C,Wenger A,Siegrist HH,Francioli P

更新日期：1994-10-01 00:00:00

abstract：:The PLEX-ID system is a novel technology that couples PCR amplification and electrospray ionization-mass spectrometry to identify pathogens directly in clinical specimens. The analytical performance of the PLEX-ID Broad Fungal assay was compared with that of traditional culture identification by using 91 characterized...

journal_title：Journal of clinical microbiology

authors： Simner PJ,Uhl JR,Hall L,Weber MM,Walchak RC,Buckwalter S,Wengenack NL

更新日期：2013-06-01 00:00:00

abstract：:Toxin-specific enzyme immunoassays, cytotoxicity assays, and PCR were used to analyze 48 toxin A-negative, toxin B-positive Clostridium difficile isolates from various geographical sites around the world. All the isolates were negative by the TOX-A TEST and positive by the TOX A/B TEST. A deletion of approximately 1.7...

journal_title：Journal of clinical microbiology

authors： Moncrief JS,Zheng L,Neville LM,Lyerly DM

更新日期：2000-08-01 00:00:00

abstract：:Yersinia pestis is the causative agent of plague and is considered a category A priority pathogen due to its potential for high transmissibility and the significant morbidity and mortality it causes in humans. Y. pestis is endemic to the western United States and much of the world, necessitating programs to monitor fo...

journal_title：Journal of clinical microbiology

authors： Chandler JC,Baeten LA,Griffin DL,Gidlewski T,DeLiberto TJ,Petersen JM,Pappert R,Young JW,Bevins SN

更新日期：2018-06-25 00:00:00

abstract：:Characterization of 102 Haemophilus strains by the Minitek differentiation system (BBL Microbiology Systems, Cockeysville, Md.) was compared with that by the conventional method. The results obtained by the two methods were in good agreement (P less than 0.001; test of independence). Therefore, this system was found t...

journal_title：Journal of clinical microbiology

authors： Kawakami Y,Okimura Y,Kanai M

更新日期：1981-11-01 00:00:00

abstract：:Mupirocin is a topical antimicrobial used to eradicate methicillin-resistant Staphylococcus aureus (MRSA) colonization, usually in the absence of susceptibility testing. We hypothesized that high-level (HL) mupirocin resistance was associated with multidrug resistance (MDR). To this end, unique patient isolates identi...

journal_title：Journal of clinical microbiology

authors： Cadilla A,David MZ,Daum RS,Boyle-Vavra S

更新日期：2011-01-01 00:00:00

abstract：:DNA colony hybridization with three 18- to 20-base-long synthetic oligonucleotide probes for cholera toxin (CT) was used to screen 12 clinical isolates of Aeromonas hydrophila. Under stringent hybridizing (overnight at 40 degrees C) and washing (1 h at 50 degrees C) conditions, nine strains reacted with the 32P-labele...

journal_title：Journal of clinical microbiology

authors： Schultz AJ,McCardell BA

更新日期：1988-01-01 00:00:00

abstract：:We assessed neutralizing antibody titers to adenovirus serotype 5 (Ad5) and six rare adenovirus serotypes, serotypes 11, 35, 50, 26, 48, and 49, in pediatric populations in sub-Saharan Africa. We observed a clear age dependence of Ad5-specific neutralizing antibody titers. These data will help to guide the development...

journal_title：Journal of clinical microbiology

authors： Thorner AR,Vogels R,Kaspers J,Weverling GJ,Holterman L,Lemckert AA,Dilraj A,McNally LM,Jeena PM,Jepsen S,Abbink P,Nanda A,Swanson PE,Bates AT,O'Brien KL,Havenga MJ,Goudsmit J,Barouch DH

更新日期：2006-10-01 00:00:00

abstract：:A rapid (2.5 h) direct enzyme immunoassay (EIA) for Clostridium difficile toxin A was developed for clinical use. Specimen centrifugation and filtration were not required. The EIA detected toxin A levels in patient stool as low as 20 pg (2 ng/ml of stool). The test was 5,000 times more sensitive for toxin A than it wa...

journal_title：Journal of clinical microbiology

authors： DiPersio JR,Varga FJ,Conwell DL,Kraft JA,Kozak KJ,Willis DH

更新日期：1991-12-01 00:00:00

abstract：:A rapid dot blot hybridization assay for the detection of B19 parvovirus DNA in human sera was developed. Small portions of four serum samples were mixed, filtered onto a nylon membrane, and hybridized with a digoxigenin-labeled DNA probe; for each membrane, 380 serum samples could be tested. When a dot was positive b...

journal_title：Journal of clinical microbiology

authors： Zerbini M,Musiani M,Venturoli S,Gallinella G,Gibellini D,Gentilomi G,La Placa M

更新日期：1990-11-01 00:00:00

abstract：:The human herpesvirus 6 (HHV-6) early protein, p41, encoded by the U27 gene has been detected in oligodendrocytes of multiple sclerosis (MS) patients by using a monoclonal antibody (MAb to p41/38). We here report the antigenic epitope of HHV-6 p41 recognized by this MAb. First, we established that the MAb to p41/38 re...

journal_title：Journal of clinical microbiology

authors： Xu Y,Linde A,Dahl H,Winberg G

更新日期：2001-04-01 00:00:00

abstract：:In contrast to cervical cancer, integration of human papillomavirus (HPV) DNA into the host genome has been considered a rare event in cancer precursor lesions (cervical intraepithelial neoplasia [CIN]). With our new real-time PCR method, we demonstrated that integrated HPV type 16 (HPV16) is already present in CIN le...

journal_title：Journal of clinical microbiology

authors： Peitsaro P,Johansson B,Syrjänen S

更新日期：2002-03-01 00:00:00

abstract：:Current methods for the diagnosis of melioidosis are based on bacteriological culture. A number of serological tests currently available lack specificity and sensitivity. This is largely due to the use of crude antigens which results in a significant cross-reactivity with sera from individuals infected with other bact...

journal_title：Journal of clinical microbiology

authors： Anuntagool N,Rugdech P,Sirisinha S

更新日期：1993-05-01 00:00:00

abstract：:Astrovirus infections were detected by enzyme immunoassay in 12 (5%) of 251 stool samples from children with gastroenteritis from Bogota, Colombia. In addition, astroviruses were detected by reverse transcription-PCR in 3 (10%) of 29 stool samples negative for other enteric pathogens collected in Caracas, Venezuela, f...

journal_title：Journal of clinical microbiology

authors： Medina SM,Gutierrez MF,Liprandi F,Ludert JE

更新日期：2000-09-01 00:00:00

abstract：:Accurate identification of mycobacterial species and subspecies is essential to evaluate their significance and to perform epidemiological studies. The subspecies of Mycobacterium avium have different attributes but coincide in their zoonotic potential. Our knowledge about M. avium subsp. silvaticum is limited, since ...

journal_title：Journal of clinical microbiology

authors： Rónai Z,Csivincsik Á,Dán Á

更新日期：2015-05-01 00:00:00

abstract：:Serogrouping of Bacteroides nodosus is based on antigenic differences in fimbriae of the different New Zealand prototype strains. Because of the time needed to isolate and grow pure cultures of B. nodosus and the difficulty in distinguishing between different serogroups because of cross-agglutination, a new DNA-based ...

journal_title：Journal of clinical microbiology

authors： John GH,Carlson JO,Kimberling CV,Ellis RP

更新日期：1990-11-01 00:00:00

abstract：:In an evaluation of the API 20NE for the identification of Burkholderia spp., 792/800 (99%) Burkholderia pseudomallei and 17/19 (89%) B. cepacia isolates were correctly identified but 10 B. mallei and 98 B. thailandensis isolates were not correctly identified. A latex agglutination test was positive for 796/800 (99.5%...

journal_title：Journal of clinical microbiology

authors： Amornchai P,Chierakul W,Wuthiekanun V,Mahakhunkijcharoen Y,Phetsouvanh R,Currie BJ,Newton PN,van Vinh Chau N,Wongratanacheewin S,Day NP,Peacock SJ

更新日期：2007-11-01 00:00:00

abstract：:Rapidly growing mycobacteria are rarely found in central nervous system infections. We describe a case of polymicrobial infection in a brain abscess including two rapidly growing Mycobacterium species, M. immunogenum and M. llatzerense. The Mycobacterium isolates were distinguishable by molecular methods, and whole-ge...

journal_title：Journal of clinical microbiology

authors： Greninger AL,Langelier C,Cunningham G,Keh C,Melgar M,Chiu CY,Miller S

更新日期：2015-07-01 00:00:00

abstract：:Herpes infections are among the most common sexually transmitted diseases and are the most common cause of genital ulcer disease in the United States. This study addresses the changing distribution of herpes simplex virus type 1 (HSV-1) and HSV-2 in patients presenting for evaluation of herpetic infections. Viral cult...

journal_title：Journal of clinical microbiology

authors： Ribes JA,Steele AD,Seabolt JP,Baker DJ

更新日期：2001-09-01 00:00:00

abstract：:The results of anaerobic susceptibility tests performed with inocula prepared directly from agar isolation media and from overnight broth cultures were compared. Altogether, 93.0% of the results with these two inoculum preparations were within one twofold dilution, including 92.5 and 95.6% of the results with Clostrid...

journal_title：Journal of clinical microbiology

authors： Murray PR,Niles AC

更新日期：1983-09-01 00:00:00

abstract：:Samples from patients at high risk for invasive aspergillosis (IA) were prospectively collected and analyzed for the presence of molecular markers of fungal infection. Serum specimens were screened for galactomannan and Aspergillus DNA, and whole-blood specimens were screened only for Aspergillus DNA. Fungal infection...

journal_title：Journal of clinical microbiology

authors： Springer J,Morton CO,Perry M,Heinz WJ,Paholcsek M,Alzheimer M,Rogers TR,Barnes RA,Einsele H,Loeffler J,White PL

更新日期：2013-05-01 00:00:00

abstract：:First-catch early morning urine samples from patients attending a genitourinary medicine clinic were tested by three different enzyme immunoassays. The results suggest significant differences in the sensitivities and specificities of the different assays. The direct visualization of elementary bodies in urine deposits...

journal_title：Journal of clinical microbiology

authors： Paul ID,Caul EO

更新日期：1990-02-01 00:00:00