Value of enterobacterial repetitive intergenic consensus PCR for study of Pasteurella multocida strains isolated from mouths of dogs.

abstract：:Serological markers for hepatitis B virus in male homosexuals demonstrated a high prevalence of past and present infection. Seropositivity of 91% for hepatitis B e antigen or antibody was demonstrated by radioimmunoassay in hepatitis B surface antigen-positive specimens. ...

journal_title：Journal of clinical microbiology

authors： Murphy BL,Schreeder MT,Maynard JE,Hadler SC,Sheller MJ

更新日期：1980-03-01 00:00:00

abstract：:Results of a 2003 survey carried out in Italy to evaluate the prevalence of extended-spectrum beta-lactamase (ESBL)-producing enterobacteria are presented. Eleven Italian Microbiology Laboratories investigated 9,076 consecutive nonreplicate isolates (inpatients, 6,850; outpatients, 2,226). ESBL screening was performed...

journal_title：Journal of clinical microbiology

authors： Luzzaro F,Mezzatesta M,Mugnaioli C,Perilli M,Stefani S,Amicosante G,Rossolini GM,Toniolo A

更新日期：2006-05-01 00:00:00

abstract：:A reversed passive latex agglutination (RPLA) assay for determining the toxigenicity of Corynebacterium diphtheriae is presented. Rabbit antitoxin antiserum was raised by using commercially available diphtheria toxoid. This antiserum reacted with the diphtheria toxin when the culture supernatant was assayed by Western...

journal_title：Journal of clinical microbiology

authors： Toma C,Sisavath L,Iwanaga M

更新日期：1997-12-01 00:00:00

abstract：:We report an uncommon clinical presentation of a unique case of fatal invasive fungal cerebral vasculitis due to Arthrographis kalrae in a nonimmunocompromised host. The identity of the fungus was determined by morphological characteristics and by analysis of internal transcribed spacer 1 sequences and was confirmed b...

journal_title：Journal of clinical microbiology

authors： Pichon N,Ajzenberg D,Desnos-Ollivier M,Clavel M,Gantier JC,Labrousse F

更新日期：2008-09-01 00:00:00

abstract：:Of 12,321 stool samples analyzed over a 6-year interval, 870 (7.1%) were positive for a total of 1,019 parasites, of which 1,011 (99.2%) were found in trichrome-stained smears of unconcentrated specimens while only 479 (47.0%) were detected in iodine-stained smears of concentrated samples. Stool specimens were next an...

journal_title：Journal of clinical microbiology

authors： Kellogg JA,Elder CJ

更新日期：1999-03-01 00:00:00

abstract：:The incidence of enterotoxigenic Escherichia coli (ETEC) was investigated in 95 E. coli strains isolated from 48 infants with diarrhea in Santiago, Chile. By using standard biological assays and DNA-DNA hybridization procedures, ETEC was found in 31.2% of the cases: 14 strains produced heat-stable enterotoxin (ST) onl...

journal_title：Journal of clinical microbiology

authors： Agüero ME,Reyes L,Prado V,Orskov I,Orskov F,Cabello FC

更新日期：1985-10-01 00:00:00

abstract：:We studied the use of dried spots of bodily fluids (plasma, whole blood, and mother's milk) on filter paper as a means of sample collection and storage for human immunodeficiency virus type 1 (HIV-1) viral load testing under stringent field conditions. Plasma placed directly in lysis buffer, which is customarily used ...

journal_title：Journal of clinical microbiology

authors： Ayele W,Schuurman R,Messele T,Dorigo-Zetsma W,Mengistu Y,Goudsmit J,Paxton WA,de Baar MP,Pollakis G

更新日期：2007-03-01 00:00:00

abstract：:The name "Campylobacter hyointestinalis" sp. nov. is proposed for a Campylobacter species that was isolated from the intestines of pigs with proliferative enteritis. "C. hyointestinalis" is also found in the feces of cattle and has been isolated from the intestine of a hamster. "C. hyointestinalis" is distinguished fr...

journal_title：Journal of clinical microbiology

authors： Gebhart CJ,Edmonds P,Ward GE,Kurtz HJ,Brenner DJ

更新日期：1985-05-01 00:00:00

abstract：:Serogrouping of Bacteroides nodosus is based on antigenic differences in fimbriae of the different New Zealand prototype strains. Because of the time needed to isolate and grow pure cultures of B. nodosus and the difficulty in distinguishing between different serogroups because of cross-agglutination, a new DNA-based ...

journal_title：Journal of clinical microbiology

authors： John GH,Carlson JO,Kimberling CV,Ellis RP

更新日期：1990-11-01 00:00:00

abstract：:Tick-borne diseases, due to a diversity of bacterial pathogens, represent a significant and increasing public health threat throughout the Northern Hemisphere. A high-throughput 16S V1-V2 rRNA gene-based metagenomics assay was developed and evaluated using >13,000 residual samples from patients suspected of having tic...

journal_title：Journal of clinical microbiology

authors： Kingry L,Sheldon S,Oatman S,Pritt B,Anacker M,Bjork J,Neitzel D,Strain A,Berry J,Sloan L,Respicio-Kingry L,Dietrich E,Bloch K,Moncayo A,Srinivasamoorthy G,Hu B,Hinckley A,Mead P,Kugeler K,Petersen J

更新日期：2020-10-21 00:00:00

abstract：:Carbapenem-resistant Acinetobacter baumannii isolates were obtained from 24 patients between March and May 2004 at the Centre Hospitalier de Polynésie Française, Tahiti, French Polynesia. The isolates were multidrug resistant, produced the carbapenemase OXA-23, and belonged to a single clone presenting several subtype...

journal_title：Journal of clinical microbiology

authors： Naas T,Levy M,Hirschauer C,Marchandin H,Nordmann P

更新日期：2005-09-01 00:00:00

abstract：:BBL CHROMagar VanRE (CVRE) was compared with bile esculin azide agar plus vancomycin to screen for vancomycin-resistant enterococcus (VRE) colonization. CVRE distinguishes Enterococcus faecalis (green colonies) from Enterococcus faecium (mauve colonies) on the basis of chromogenic substrate use. CVRE sensitivity and s...

journal_title：Journal of clinical microbiology

authors： Kallstrom G,Doern CD,Dunne WM Jr

更新日期：2010-03-01 00:00:00

abstract：:During a 6-month study we critically evaluated the accuracy of the AutoMicrobic system Gram-Negative Identification Card (Vitek Systems, Inc., Hazelwood, Mo.) in identifying glucose-nonfermenting gram-negative bacilli by testing 419 selected isolates in parallel with a conventional reference method. Of 356 isolates in...

journal_title：Journal of clinical microbiology

authors： Plorde JJ,Gates JA,Carlson LG,Tenover FC

更新日期：1986-02-01 00:00:00

abstract：:A new test principle for the simultaneous detection of total approximate titers and immunoglobulin M antibodies has been developed and applied to the detection of antibody to hepatitis B core antigen. The method is based on the combination of a competition radioimmunoassay, for the determination of total antibody tite...

journal_title：Journal of clinical microbiology

authors： Angarano G,Monno L,Santantonio TA,Pastore G

更新日期：1984-06-01 00:00:00

abstract：:Of 35 stool specimens isolated and examined in 293 cells, 15 isolates contained adenovirus species 40 (Ad40), and 4 of these 15 isolates also contained a nonfastidious adenovirus species (Ad1 in two cases, Ad18 or Ad31) which was selected over Ad40 during serial passage in the 293 cells. The selection of Ad1 over Ad40...

journal_title：Journal of clinical microbiology

authors： Brown M

更新日期：1985-08-01 00:00:00

abstract：:In the present study, a novel broad-range real-time PCR was developed for the rapid detection of human pathogenic fungi. The assay targets a part of the 28S large-subunit ribosomal RNA (rDNA) gene. We investigated its application for the most important human pathogenic fungal genera, including Aspergillus, Candida, Cr...

journal_title：Journal of clinical microbiology

authors： Vollmer T,Störmer M,Kleesiek K,Dreier J

更新日期：2008-06-01 00:00:00

abstract：:The rates of infection with Fusarium molds are increasing, and a diverse number of Fusarium spp. belonging to different species complexes can cause infection. Conventional species identification in the clinical laboratory is time-consuming and prone to errors. We therefore evaluated whether matrix-assisted laser desor...

journal_title：Journal of clinical microbiology

authors： Triest D,Stubbe D,De Cremer K,Piérard D,Normand AC,Piarroux R,Detandt M,Hendrickx M

更新日期：2015-02-01 00:00:00

abstract：:Between 1998 and 2007, records from 33 patients with cutaneous diphtheria from Vancouver's inner city were reviewed. Cases were associated with injection drug use and poverty. Coinfections with Staphylococcus aureus, Streptococcus pyogenes, and Arcanobacterium haemolyticum occurred. Corynebacterium diphtheriae is ende...

journal_title：Journal of clinical microbiology

authors： Lowe CF,Bernard KA,Romney MG

更新日期：2011-07-01 00:00:00

abstract：:In this study, we determined the sequence of the EcoRI-PstI fragment of the plasmid pCVD432, also termed the enteroaggregative Escherichia coli (EAggEC) probe. A primer pair complementary to this probe was designed for PCR amplification of a 630-bp region. Comparison of the analysis of the EAggEC probe sequence with t...

journal_title：Journal of clinical microbiology

authors： Schmidt H,Knop C,Franke S,Aleksic S,Heesemann J,Karch H

更新日期：1995-03-01 00:00:00

abstract：:We have studied 221 adults drawn from an impoverished urban population with high human immunodeficiency virus (HIV) seroprevalence (35%) to determine the prevalence of gastroduodenal pathology and its relationship to serological markers of Helicobacter pylori virulence proteins and other potential environmental and im...

journal_title：Journal of clinical microbiology

authors： Fernando N,Holton J,Zulu I,Vaira D,Mwaba P,Kelly P

更新日期：2001-04-01 00:00:00

abstract：:mariPOC is a novel point-of-care test system for rapid detection of respiratory tract infections. We compared the performance of the mariPOC test to that of bacterial culture for detecting group A streptococcus (GAS) in 219 pharyngitis patients (ages 1-64 years) and 109 healthy asymptomatic controls (ages 19-69 years)...

journal_title：Journal of clinical microbiology

authors： Vakkila J,Koskinen JO,Brandt A,Muotiala A,Liukko V,Soittu S,Meriluoto S,Vesalainen M,Huovinen P,Irjala K

更新日期：2015-07-01 00:00:00

abstract：:We compared a nested PCR assay and microscopic examination of Giemsa-stained blood films for detection and identification of Plasmodium spp. in blood specimens. PCR was more sensitive than microscopy and capable of identifying malaria parasites at the species level when microscopy was equivocal. ...

journal_title：Journal of clinical microbiology

authors： Johnston SP,Pieniazek NJ,Xayavong MV,Slemenda SB,Wilkins PP,da Silva AJ

更新日期：2006-03-01 00:00:00

abstract：:Bartonella henselae uveitis was diagnosed in a 40-year-old woman with underlying HLA-B27 uveitis on the basis of immunodetection and molecular detection of the organism, a Marseilles genogroup and a CAL-1 genotype strain, in the vitreous fluid. This case illustrates that B. henselae should be included in the different...

journal_title：Journal of clinical microbiology

authors： Drancourt M,Bodaghi B,Lepidi H,Le Hoang P,Raoult D

更新日期：2004-04-01 00:00:00

abstract：:We have used a polymerase chain reaction (PCR) to examine cervical cancer biopsy specimens and pelvic lymph nodes for the presence of human papillomavirus type 16 (HPV 16) DNA. Of the 75 cervical specimens tested, 36 (48%) were positive for HPV 16 in the PCR. A total of 65 pelvic lymph nodes removed during radical sur...

journal_title：Journal of clinical microbiology

authors： Czeglédy J,Póka R,Veress G,Gergely L

更新日期：1992-01-01 00:00:00

abstract：:The importance of euparal as a permanent mounting medium for eggs of parasites and the process of making permanent slides are analyzed and discussed in detail. Studies show that helminth eggs mounted in euparal exhibit excellent optical and drying properties. Euparal also can be used to identify proglottids of Taenia ...

journal_title：Journal of clinical microbiology

authors： Berlin OG,Miller MJ

更新日期：1980-11-01 00:00:00

abstract：:A simple enzyme immunoassay has been developed to allow the quantitation of the human response to immunization with pneumococcal polysaccharide. The assay uses the 14-valent vaccine (Pneumovax) as a convenient antigen to adsorb to the solid-phase microdilution plate wells and commercially available isotype-specific an...

journal_title：Journal of clinical microbiology

authors： Shyamala GN,Roberton DM,Hosking CS

更新日期：1988-08-01 00:00:00

abstract：:Although vaginitis caused by Saccharomyces cerevisiae is extremely rare, in recent years we have experienced an increasing frequency of S. cerevisiae isolation from the vaginas of fertile-age women. In order to investigate the epidemiology of these vaginal infections, a total of 40 isolates of S. cerevisiae derived fr...

journal_title：Journal of clinical microbiology

authors： Posteraro B,Sanguinetti M,D'Amore G,Masucci L,Morace G,Fadda G

更新日期：1999-07-01 00:00:00

abstract：:Multidrug-resistant (MDR) Klebsiella pneumoniae is one of the most important causes of nosocomial infections worldwide. After the spread of strains resistant to beta-lactams at the end of the previous century, the diffusion of isolates resistant to carbapenems and colistin is now reducing treatment options and the con...

journal_title：Journal of clinical microbiology

authors： Onori R,Gaiarsa S,Comandatore F,Pongolini S,Brisse S,Colombo A,Cassani G,Marone P,Grossi P,Minoja G,Bandi C,Sassera D,Toniolo A

更新日期：2015-09-01 00:00:00

abstract：:In a collaborative study at three university hospitals, the recovery of microorganisms and the speed of detection of microbial growth by the BacT/Alert (Organon Teknika Corporation, Durham, N.C.) and BACTEC 660/730 (Becton-Dickinson Diagnostic Instrument Systems, Sparks, Md.) nonradiometric blood culture systems were ...

journal_title：Journal of clinical microbiology

authors： Wilson ML,Weinstein MP,Reimer LG,Mirrett S,Reller LB

更新日期：1992-02-01 00:00:00

abstract：:Recent reports of natural paratuberculosis (or Johne's disease) in rabbits, foxes, and stoats has focused debate on the presence and importance of wildlife reservoirs in the epidemiology of this disease. This paper describes an extensive study investigating 18 nonruminant wildlife species for evidence of paratuberculo...

journal_title：Journal of clinical microbiology

authors： Beard PM,Daniels MJ,Henderson D,Pirie A,Rudge K,Buxton D,Rhind S,Greig A,Hutchings MR,McKendrick I,Stevenson K,Sharp JM

更新日期：2001-04-01 00:00:00