Molecular and epidemiological characterization of vaginal Saccharomyces cerevisiae isolates.

abstract：:A total of 192 hybridomas were developed from mice immunized with Rickettsia japonica, a newly identified spotted fever group rickettsia pathogenic for humans. Of these hybridomas, 101 were species specific, 37 were spotted fever group reactive, and the other 54 were also reactive with one or more of the other pathoge...

journal_title：Journal of clinical microbiology

authors： Uchiyama T,Uchida T,Walker DH

更新日期：1990-06-01 00:00:00

abstract：:The diagnosis of brucellosis in cattle and small ruminants requires the use of more than one serological test. The complement fixation test (CFT), the rose bengal test (RBT), and the serum agglutination test (SAT) are among the most useful tests for routine diagnosis. The microagglutination test (MAT) was developed as...

journal_title：Journal of clinical microbiology

authors： Baum M,Zamir O,Bergman-Rios R,Katz E,Beider Z,Cohen A,Banai M

更新日期：1995-08-01 00:00:00

abstract：:The factors that regulate cytomegalovirus (CMV) excretion from the genitourinary tract are poorly understood. To assess the role of cell-mediated immunity in such excretion, a CMV-specific mononuclear blastogenesis assay was used to study a predominantly lower-socioeconomic-status population of 92 healthy nonpregnant ...

journal_title：Journal of clinical microbiology

authors： Faix RG,Zweig SE,Kummer JF,Moore D,Lang DJ

更新日期：1983-04-01 00:00:00

abstract：:Carbohydrate antigen detection, nucleic acid probe detection, and bacterial culture are commonly used to confirm group A streptococcus (GAS) pharyngitis. Compared to standard throat swab specimens, the sensitivities of these tests with mouth specimens are poor. When testing for GAS pharyngitis, the throat remains the ...

journal_title：Journal of clinical microbiology

authors： Fox JW,Marcon MJ,Bonsu BK

更新日期：2006-07-01 00:00:00

abstract：:The epidemiological implications of the recent separation of "Entamoeba histolytica" into two separate species, pathogenic E. histolytica sensu stricto and commensal E. dispar, will not become apparent without methods of distinguishing between them which are applicable to large numbers of specimens. We have modified a...

journal_title：Journal of clinical microbiology

authors： Britten D,Wilson SM,McNerney R,Moody AH,Chiodini PL,Ackers JP

更新日期：1997-05-01 00:00:00

abstract：:Twenty-three nasal swab samples that tested positive for methicillin-resistant Staphylococcus aureus (MRSA) on initial testing by the BD GeneOhm MRSA assay (BD-MRSA PCR; BD GeneOhm, San Diego, CA) were culture positive only for methicillin-susceptible S. aureus (MSSA) from an enrichment broth. The 23 recovered isolate...

journal_title：Journal of clinical microbiology

authors： Stamper PD,Louie L,Wong H,Simor AE,Farley JE,Carroll KC

更新日期：2011-04-01 00:00:00

abstract：:The increasing number of multidrug-resistant Mycobacterium tuberculosis strains has stimulated the interest of investigators in finding a rapid method for susceptibility testing. We used commercially available rRNA DNA-bioluminescence-labelled probes (Accu-Probe, Gen Probe, Inc. San Diego, Calif.) for this purpose. Th...

journal_title：Journal of clinical microbiology

authors： Martin-Casabona N,Xairó Mimó D,González T,Rossello J,Arcalis L

更新日期：1997-10-01 00:00:00

abstract：:We have evaluated the diagnostic utility of eleven Toxoplasma gondii recombinant antigens (P22 [SAG2], P24 [GRA1], P25, P28 [GRA2], P29 [GRA7], P30 [SAG1], P35, P41 [GRA4], P54 [ROP2], P66 [ROP1], and P68) in immunoglobulin G (IgG) and IgM recombinant enzyme-linked immunosorbent assays (Rec-ELISAs). Following an initi...

journal_title：Journal of clinical microbiology

authors： Aubert D,Maine GT,Villena I,Hunt JC,Howard L,Sheu M,Brojanac S,Chovan LE,Nowlan SF,Pinon JM

更新日期：2000-03-01 00:00:00

abstract：:A group of 120 sera from blood donors was screened by complement fixation and commercially available immunofluorescence, solid-phase fluorescence immunoassay, enzyme-linked immunosorbent assay, and indirect hemagglutination tests. Twenty-four of the sera were positive by three or more of the five tests and judged to b...

journal_title：Journal of clinical microbiology

authors： Phipps PH,Grégoire L,Rossier E,Perry E

更新日期：1983-12-01 00:00:00

abstract：:In this work, we show that antibodies to baculovirus recombinant DNA polymerase of human cytomegalovirus are present in human sera during natural infection. Specific antibody was found to be of the immunoglobulin G class, produced at low titers only for a short period of time at the beginning of acute infection and no...

journal_title：Journal of clinical microbiology

authors： Landini MP,Lazzarotto T,Ertl PF

更新日期：1993-03-01 00:00:00

abstract：:Gas-liquid chromatographic (GLC) profiles of cellular fatty acids and metabolic products were useful in identifying strains of Peptococcus saccharolyticus, Peptococcus asaccharolyticus, Peptostreptococcus anaerobius, Peptostreptococcus micros, and Streptococcus intermedius. The GLC results supported the recent taxonom...

journal_title：Journal of clinical microbiology

authors： Lambert MA,Armfield AY

更新日期：1979-10-01 00:00:00

abstract：:This study evaluates the performance of a PCR assay for the detection of Pneumocystis carinii from respiratory specimens that has been designed for use in the clinical microbiology laboratory. The test includes a simple method for nucleic acid extraction and amplification, a colorimetric probe hybridization technique ...

journal_title：Journal of clinical microbiology

authors： Caliendo AM,Hewitt PL,Allega JM,Keen A,Ruoff KL,Ferraro MJ

更新日期：1998-04-01 00:00:00

abstract：:The performance of a direct sandwich enzyme-linked immunosorbent assay (ELISA) for detecting Aspergillus galactomannan was compared with that of the Pastorex Aspergillus antigen latex agglutination (LA) test by using 532 serum samples from 61 patients at risk for invasive aspergillosis. The ELISA gave positive results...

journal_title：Journal of clinical microbiology

authors： Verweij PE,Stynen D,Rijs AJ,de Pauw BE,Hoogkamp-Korstanje JA,Meis JF

更新日期：1995-07-01 00:00:00

abstract：:The analytical performance of the AIX1000 system, a fully automated and recently FDA-cleared rapid plasma reagin (RPR) system, was evaluated by comparison to a manual RPR test in a traditional syphilis testing algorithm. A total of 1,028 consecutive serum samples submitted for syphilis testing to the University of Nor...

journal_title：Journal of clinical microbiology

authors： Sanfilippo AM,Freeman K,Schmitz JL

更新日期：2018-07-26 00:00:00

abstract：:The value of immunoglobulin M (IgM) detection in the early diagnosis of influenza A was examined in a prospective study during an outbreak of influenza A/Philippines/2/82 (H3N2) virus infection in February and March 1986. The IgM response was investigated in sera from 64 adults with influenzalike symptoms; we found a ...

journal_title：Journal of clinical microbiology

authors： Vikerfors T,Lindegren G,Grandien M,van der Logt J

更新日期：1989-03-01 00:00:00

abstract：:A set of 104 isolates from human clinical samples from the United States, morphologically compatible with Bipolaris, were morphologically and molecularly identified through the sequence analysis of the internal transcribed space (ITS) region of the nuclear ribosomal DNA (rDNA). The predominant species was Bipolaris sp...

journal_title：Journal of clinical microbiology

authors： da Cunha KC,Sutton DA,Fothergill AW,Cano J,Gené J,Madrid H,De Hoog S,Crous PW,Guarro J

更新日期：2012-12-01 00:00:00

abstract：:A new approach, short-oligonucleotide-ligation assay on DNA chip (SOLAC), is developed to detect mutations in rifampin-resistant Mycobacterium tuberculosis. The method needs only four common probes to detect 15 mutational variants of the rpoB gene within 12 h. Fifty-five rifampin-resistant M. tuberculosis isolates wer...

journal_title：Journal of clinical microbiology

authors： Deng JY,Zhang XE,Lu HB,Liu Q,Zhang ZP,Zhou YF,Xie WH,Fu ZJ

更新日期：2004-10-01 00:00:00

abstract：:We compared the new Abbott TestPack (TP) respiratory syncytial virus (RSV) enzyme immunoassay (EIA) with cell culture and two commercial RSV EIAs (from Abbott Diagnostics and Kallestad Laboratories) by using split samples of fresh nasal washings from children with suspected RSV disease. Two tubes of HEp-2 cells were i...

journal_title：Journal of clinical microbiology

authors： Wren CG,Bate BJ,Masters HB,Lauer BA

更新日期：1990-06-01 00:00:00

abstract：:Malaria is one of the leading causes of infectious disease in travelers returning from the tropics. The diagnosis of malaria is typically performed by examining Giemsa-stained thick and thin peripheral blood smears, which is time consuming, labor intensive, and requires high levels of proficiency. Alternatively, loop-...

journal_title：Journal of clinical microbiology

authors： Rypien C,Chow B,Chan WW,Church DL,Pillai DR

更新日期：2017-10-01 00:00:00

abstract：:Although numerous perioperative samples and culture media are required to diagnose prosthetic joint infection (PJI), their exact number and types have not yet been definitely determined with a high level of proof. We conducted a prospective multicenter study to determine the minimal number of samples and culture media...

journal_title：Journal of clinical microbiology

authors： Bémer P,Léger J,Tandé D,Plouzeau C,Valentin AS,Jolivet-Gougeon A,Lemarié C,Kempf M,Héry-Arnaud G,Bret L,Juvin ME,Giraudeau B,Corvec S,Burucoa C,Centre de Référence des Infections Ostéo-articulaires du Grand Ouest (CRIOGO) Stu

更新日期：2016-02-01 00:00:00

abstract：:Acute and convalescent-phase sera obtained from 10 patients infected with a Japanese strain of spotted fever group (SFG) rickettsia were tested by the indirect immunoperoxidase test, the Weil-Felix test, an enzyme-linked immunosorbent assay (ELISA), and immunoblotting. By the Weil-Felix test, the reactivity of these s...

journal_title：Journal of clinical microbiology

authors： Amano K,Hatakeyama H,Okuta M,Suto T,Mahara F

更新日期：1992-09-01 00:00:00

abstract：:During a 6-month study we critically evaluated the accuracy of the AutoMicrobic system Gram-Negative Identification Card (Vitek Systems, Inc., Hazelwood, Mo.) in identifying glucose-nonfermenting gram-negative bacilli by testing 419 selected isolates in parallel with a conventional reference method. Of 356 isolates in...

journal_title：Journal of clinical microbiology

authors： Plorde JJ,Gates JA,Carlson LG,Tenover FC

更新日期：1986-02-01 00:00:00

abstract：:Metarhizium viride has been associated with fatal systemic mycoses in chameleons, but subsequent data on mycoses caused by this fungus in reptiles are lacking. The aim of this investigation was therefore to obtain information on the presence of M. viride in reptiles kept as pets in captivity and its association with c...

journal_title：Journal of clinical microbiology

authors： Schmidt V,Klasen L,Schneider J,Hübel J,Pees M

更新日期：2017-03-01 00:00:00

abstract：:Twenty-two isolates of St. Louis encephalitis (SLE) virus of various geographical origins (Brazil, Argentina, Panama, Texas, Missouri, Maryland, California, and Florida) were examined for genetic variation by the base excision sequence scanning (BESS T-scan) method. A fragment was amplified in the envelope gene with t...

journal_title：Journal of clinical microbiology

authors： Charrel RN,Lévy N,Tesh RB,Chandler LJ

更新日期：1999-06-01 00:00:00

abstract：:Direct enzyme immunoassay (Herpchek) was compared with culture on 21,522 specimens mainly from asymptomatic women for herpes simplex virus detection. Sensitivity and specificity were 73.8 and 97.7%, respectively. The 33% detection rate by enzyme immunoassay in 5 h increased to 43% when the enzyme immunoassay was combi...

journal_title：Journal of clinical microbiology

authors： Verano L,Michalski FJ

更新日期：1995-05-01 00:00:00

abstract：:We compared 75 nontypeable (NT) Haemophilus influenzae isolates by pulsed-field gel electrophoresis (PFGE), enterobacterial repetitive intergenic consensus (ERIC)-PCR, and automated ribotyping. PFGE was the most discriminatory of the techniques. ERIC-PCR provides a useful screen but should not replace other techniques...

journal_title：Journal of clinical microbiology

authors： Pettigrew MM,Foxman B,Ecevit Z,Marrs CF,Gilsdorf J

更新日期：2002-02-01 00:00:00

abstract：:Eight strains of a new intermediate adenovirus were isolated in the course of an investigation of conjunctivitis in Hiroshima City, Japan. The strain was first isolated in July 1986. All eight strains were isolated from conjunctival swab samples from patients with conjunctivitis, mainly epidemic keratoconjunctivitis. ...

journal_title：Journal of clinical microbiology

authors： Noda M,Miyamoto Y,Ikeda Y,Matsuishi T,Ogino T

更新日期：1991-07-01 00:00:00

abstract：:A study of immunofluorescence of yeast in the urine was carried out in 18 patients with funguria in an effort to correlate gamma globulin coating with evidence of tissue invasion or as an indicator for institution of therapy. Positive immunofluorescence of yeast in urine is common but is neither indicative of upper ur...

journal_title：Journal of clinical microbiology

authors： Everett ED,Eickhoff TC,Ehret JM

更新日期：1976-08-01 00:00:00

abstract：:Prion diseases of cattle include the classical bovine spongiform encephalopathy (C-BSE) and the atypical H-type BSE (H-BSE) and L-type BSE (L-BSE) strains. Although the C- and L-BSE strains can be detected and discriminated by ultrasensitive real-time quaking-induced conversion (RT-QuIC) assays, no such test has yet b...

journal_title：Journal of clinical microbiology

authors： Masujin K,Orrú CD,Miyazawa K,Groveman BR,Raymond LD,Hughson AG,Caughey B

更新日期：2016-03-01 00:00:00

abstract：:In the treatment of serious bacterial infections, the rapid institution of appropriate antimicrobial chemotherapy may be lifesaving. Choosing the correct antibiotic or combination of antibiotics is becoming very important, as multidrug resistance is found in many pathogens. Using a collection of 75 well-characterized ...

journal_title：Journal of clinical microbiology

authors： Hujer KM,Hujer AM,Endimiani A,Thomson JM,Adams MD,Goglin K,Rather PN,Pennella TT,Massire C,Eshoo MW,Sampath R,Blyn LB,Ecker DJ,Bonomo RA

更新日期：2009-05-01 00:00:00