An improved colorimetric PCR-based method for detection and differentiation of Entamoeba histolytica and Entamoeba dispar in feces.

abstract：:Cell-free and cell-associated human immunodeficiency virus cultures suspended in 10% serum remained infectious for several weeks at room temperature. The stability was further increased when cell-associated virus was suspended in neat serum. When dried onto a glass coverslip, virus remained infectious for several days...

journal_title：Journal of clinical microbiology

authors： van Bueren J,Simpson RA,Jacobs P,Cookson BD

更新日期：1994-02-01 00:00:00

abstract：:To identify Trichinella antigens suitable for high-specificity and high-sensitivity serodiagnosis of human trichinellosis, we evaluated assays using four antigens: (i) crude first-stage larval extract (CLE), (ii) O-deglycosylated CLE, (iii) tyvelose-bearing antigens (Trichinella spiralis larva group 1 [TSL-1] antigens...

journal_title：Journal of clinical microbiology

authors： Escalante M,Romarís F,Rodríguez M,Rodríguez E,Leiro J,Gárate MT,Ubeira FM

更新日期：2004-09-01 00:00:00

abstract：:A biotype analysis was performed on serologically nontypable Haemophilus influenzae isolates from the middle ear effusions and nasopharynges of 33 children with chronic otitis media with effusion (serous otitis media). Over 50% of the H. influenzae isolates from the middle ears belonged to biotype II. The incidence of...

journal_title：Journal of clinical microbiology

authors： DeMaria TF,Lim DJ,Barnishan J,Ayers LW,Birck HG

更新日期：1984-12-01 00:00:00

abstract：:Many laboratory workers prefer the rhodamine-auramine method of staining acid-fast bacilli (AFB) in primary specimen smears rather than carbol fuchsin stains because the stain is more readily interpreted and yields greater sensitivity. The increasing incidence of AFB infections serves as an impetus to optimize the rho...

journal_title：Journal of clinical microbiology

authors： McCarter YS,Robinson A

更新日期：1994-10-01 00:00:00

abstract：:A reverse transcriptase PCR (RT-PCR) was used for rapid determination of the hemagglutinin (HA) cleavage site sequence, a marker for the virulence potential of avian influenza viruses. When applied to specimens from chickens experimentally infected with either a virulent or an avirulent virus, RT-PCR uniformly detecte...

journal_title：Journal of clinical microbiology

authors： Horimoto T,Kawaoka Y

更新日期：1995-03-01 00:00:00

abstract：:The cellular fatty acid composition of Brucella canis and Brucella suis was determined by gas-liquid chromatography. The presence of relatively large amounts of a 19-carbon cyclopropane fatty acid in B. suis was a major distinguishing feature between these organisms. The gas-liquid chromatography test for cellular fat...

journal_title：Journal of clinical microbiology

authors： Dees SB,Hollis DG,Weaver RE,Moss CW

更新日期：1981-07-01 00:00:00

abstract：:A latex agglutination test was evaluated as a method for detection and titration of antiplague antibodies. Slide and microtiter techniques using polystyrene latex particles coated with specific fraction-I-antigen of Yersinia pestis were found to be comparable in specificity and sensitivity to serological tests commonl...

journal_title：Journal of clinical microbiology

authors： Suzuki S,Sakakibara H,Hotta S

更新日期：1977-10-01 00:00:00

abstract：:The value of immunoglobulin M (IgM) detection in the early diagnosis of influenza A was examined in a prospective study during an outbreak of influenza A/Philippines/2/82 (H3N2) virus infection in February and March 1986. The IgM response was investigated in sera from 64 adults with influenzalike symptoms; we found a ...

journal_title：Journal of clinical microbiology

authors： Vikerfors T,Lindegren G,Grandien M,van der Logt J

更新日期：1989-03-01 00:00:00

abstract：:Two strains of a previously undescribed Actinomyces-like bacterium were recovered in pure culture from infected root canals of teeth. Analysis by biochemical testing and polyacrylamide gel electrophoresis of whole-cell proteins indicated that the strains closely resembled each other phenotypically but were distinct fr...

journal_title：Journal of clinical microbiology

authors： Collins MD,Hoyles L,Kalfas S,Sundquist G,Monsen T,Nikolaitchouk N,Falsen E

更新日期：2000-09-01 00:00:00

abstract：:A modified indirect hemagglutination test for the detection of treponemal antibodies was developed for use with finger-prick blood. By using paired serum and absorbed finger-prick blood from 58 patients from an area previously endemic for yaws and 12 patients without yaws, the modified hemagglutination test was compar...

journal_title：Journal of clinical microbiology

authors： Backhouse JL,Lee MH,Nesteroff SI,Hudson BJ,Hamilton PA

更新日期：1992-03-01 00:00:00

abstract：:To our knowledge, no data are available on whether the microbial species composition and abundance sampled with self-collected vaginal swabs are comparable to those of swabs collected by clinicians. Twenty healthy women were recruited to the study during a routine gynecological visit. Eligible women were between 18 an...

journal_title：Journal of clinical microbiology

authors： Forney LJ,Gajer P,Williams CJ,Schneider GM,Koenig SS,McCulle SL,Karlebach S,Brotman RM,Davis CC,Ault K,Ravel J

更新日期：2010-05-01 00:00:00

abstract：:Forty-two difficult-to-cultivate group A coxsackieviruses (i.e., group A types other than A7, A9, and A16), collected primarily from throat swab specimens of patients suffering from fever, pharyngitis, lymphadenopathy, and cough during the 1986 enterovirus season, were isolated in less than 24-h-old suckling mice. Thi...

journal_title：Journal of clinical microbiology

authors： Lipson SM,Walderman R,Costello P,Szabo K

更新日期：1988-07-01 00:00:00

abstract：:In this work, we show that antibodies to baculovirus recombinant DNA polymerase of human cytomegalovirus are present in human sera during natural infection. Specific antibody was found to be of the immunoglobulin G class, produced at low titers only for a short period of time at the beginning of acute infection and no...

journal_title：Journal of clinical microbiology

authors： Landini MP,Lazzarotto T,Ertl PF

更新日期：1993-03-01 00:00:00

abstract：:Outbreaks of food-borne listeriosis have often involved strains of serotype 4b. Examination of multiple isolates from three different outbreaks revealed that ca. 11 to 29% of each epidemic population consisted of strains which were negative with the serotype-specific monoclonal antibody c74.22, lacked galactose from t...

journal_title：Journal of clinical microbiology

authors： Clark EE,Wesley I,Fiedler F,Promadej N,Kathariou S

更新日期：2000-10-01 00:00:00

abstract：:A new, rapid method for the detection of human immunodeficiency virus type 1 (HIV-1) antibody by time-resolved fluoroimmunoassay (TR-FIA) was developed. In this assay format, microtitration strips were coated with a recombinant fusion protein, and the same protein was labeled with europium and added into the wells sim...

journal_title：Journal of clinical microbiology

authors： Siitari H,Turunen P,Schrimsher J,Nunn M

更新日期：1990-09-01 00:00:00

abstract：:We present the case of a 77-year-old woman who developed an Actinomyces naeslundii infection of a hip prosthesis. The isolate grew well aerobically with 5% CO(2). Possible diagnostic problems may arise in the microbiological laboratory because aerobic growth is not sufficiently accounted for in some of the traditional...

journal_title：Journal of clinical microbiology

authors： Wüst J,Steiger U,Vuong H,Zbinden R

更新日期：2000-02-01 00:00:00

abstract：:Yarrowia lipolytica is a weakly pathogenic yeast that is rarely isolated from the blood. We observed transient recurrent catheter-related fungemia attributable to this organism in a leukemic patient. The fungemia and accompanying fever subsided spontaneously. The data suggest that it might be possible to withhold spec...

journal_title：Journal of clinical microbiology

authors： Chang CL,Park TH,Lee EY,Lim YT,Son HC

更新日期：2001-03-01 00:00:00

abstract：:Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been suggested as a reliable method for bacterial identification from cultures. Direct analysis of clinical samples might increase the usefulness of this method, shortening the time for microorganism identification. We comp...

journal_title：Journal of clinical microbiology

authors： Ferreira L,Sánchez-Juanes F,González-Avila M,Cembrero-Fuciños D,Herrero-Hernández A,González-Buitrago JM,Muñoz-Bellido JL

更新日期：2010-06-01 00:00:00

abstract：:Rhodotorula infections occur among patients with immunosuppression and/or central venous catheters. Using standardized methods (NCCLS M27-A), we determined the antifungal susceptibilities of 10 Rhodotorula bloodstream infection isolates. Patient information was collected for clinical correlation. The MICs of amphoteri...

journal_title：Journal of clinical microbiology

authors： Zaas AK,Boyce M,Schell W,Lodge BA,Miller JL,Perfect JR

更新日期：2003-11-01 00:00:00

abstract：:The IDI-MRSA assay (Infectio Diagnostic, Inc., Sainte-Foy, Quebec, Canada) with the Smart Cycler II rapid DNA amplification system (Cepheid, Sunnyvale, CA) appears to be sensitive and specific for the rapid detection of nasal colonization by methicillin-resistant Staphylococcus aureus (MRSA). We assessed the sensitivi...

journal_title：Journal of clinical microbiology

authors： Bishop EJ,Grabsch EA,Ballard SA,Mayall B,Xie S,Martin R,Grayson ML

更新日期：2006-08-01 00:00:00

abstract：:Since influenza viruses can cause severe illness, timely diagnosis is important for an adequate intervention. The available rapid detection methods either lack sensitivity or require complex laboratory manipulation. This study describes a rapid, sensitive detection method that can be easily applied to routine diagnosi...

journal_title：Journal of clinical microbiology

authors： van Elden LJ,Nijhuis M,Schipper P,Schuurman R,van Loon AM

更新日期：2001-01-01 00:00:00

abstract：:The DNA fingerprint profiles and somatic serotypes of 71 Pasteurella multocida capsule serogroup B isolates, 13 capsule serogroup E isolates, and 16 somatic reference serotype strains were compared. Each of the 16 reference somatic serotypes had a unique DNA fingerprint profile with the HhaI restriction endonuclease. ...

journal_title：Journal of clinical microbiology

authors： Wilson MA,Rimler RB,Hoffman LJ

更新日期：1992-06-01 00:00:00

abstract：:Diagnosis of invasive aspergillosis (IA) requires increasingly rapid molecular methods that enable sensitive detection and discrimination between species. We designed and evaluated a real-time PCR-based method that combined melting temperature (T(m)) calling analysis of a specific probe with high-resolution melting an...

journal_title：Journal of clinical microbiology

authors： Alonso M,Escribano P,Guinea J,Recio S,Simon A,Peláez T,Bouza E,García de Viedma D

更新日期：2012-10-01 00:00:00

abstract：:We report a case of Veillonella parvula lumbar discitis and secondary bacteremia confirmed by molecular characterization of the 16S rRNA genes. Identification of the organism was essential for an appropriate choice of antimicrobial therapy following the failure of empirical flucloxacillin. Veillonella spp. are normal ...

journal_title：Journal of clinical microbiology

authors： Marriott D,Stark D,Harkness J

更新日期：2007-02-01 00:00:00

abstract：:A severe outbreak of hemorrhagic colitis occurred in London, Ontario, during the month of September 1985. A total of 55 residents and 18 employees of a nursing home developed diarrhea, and 17 residents (age range, 78 to 99 years) died. Specimens from 38 patients, 37 employees and contacts, and 10 autopsies were invest...

journal_title：Journal of clinical microbiology

authors： Krishnan C,Fitzgerald VA,Dakin SJ,Behme RJ

更新日期：1987-06-01 00:00:00

abstract：:Nosocomial transmission of Neisseria meningitidis has only rarely been reported. Here, we present a significant spatiotemporal association of two cases of invasive meningococcal disease identified by retrospective cluster analysis with the program SaTScan. The most likely epidemiological link was simultaneous hospital...

journal_title：Journal of clinical microbiology

authors： Elias J,Health Office in the Rural District Office Wartburgkreis.,Claus H,Frosch M,Vogel U

更新日期：2006-11-01 00:00:00

abstract：:In specific-pathogen-free dogs experimentally infected with Borrelia burgdorferi by tick exposure, treatment with high doses of amoxicillin or doxycycline for 30 days diminished but failed to eliminate persistent infection. Although joint disease was prevented or cured in five of five amoxicillin- and five of six doxy...

journal_title：Journal of clinical microbiology

authors： Straubinger RK,Summers BA,Chang YF,Appel MJ

更新日期：1997-01-01 00:00:00

abstract：:Fluorophore-assisted carbohydrate electrophoresis (FACE) is a straightforward, sensitive method for determining the presence and relative abundance of individual oligomannosyl residues in Candida mannoprotein, the major antigenic determinant located on the outer surface of the yeast cell wall. The single terminal alde...

journal_title：Journal of clinical microbiology

authors： Goins TL,Cutler JE

更新日期：2000-08-01 00:00:00

abstract：:Bilophila wadsworthia is a common inhabitant of the human colon and has been associated with appendicitis and other local sites of inflammation in humans. Challenge-exposure or prevalence studies in laboratory and other animals have not been reported. B. wadsworthia is closely related phylogenetically to Desulfovibrio...

journal_title：Journal of clinical microbiology

authors： McOrist AL,Warhurst M,McOrist S,Bird AR

更新日期：2001-04-01 00:00:00

abstract：:Between 1 and 2% of the population in the developed world experiences a nonhealing or chronic wound characterized by an apparent arrest in a stage dominated by inflammatory processes. Lately, research groups have proposed that bacteria might be involved in and contribute to the lack of healing of these wounds. To inve...

journal_title：Journal of clinical microbiology

authors： Kirketerp-Møller K,Jensen PØ,Fazli M,Madsen KG,Pedersen J,Moser C,Tolker-Nielsen T,Høiby N,Givskov M,Bjarnsholt T

更新日期：2008-08-01 00:00:00