Abstract:
:Diagnosis of invasive aspergillosis (IA) requires increasingly rapid molecular methods that enable sensitive detection and discrimination between species. We designed and evaluated a real-time PCR-based method that combined melting temperature (T(m)) calling analysis of a specific probe with high-resolution melting analysis of the full amplicon. The test correctly identified 78 isolates of Aspergillus section Fumigati and non-Fumigati sections of Aspergillus with a limit of detection of 10(2) conidia/ml (10(2) fg/ml). No cross-reactivity with other fungi was found. The assay was further validated on lower respiratory tract specimens containing Aspergillus or not. It successfully identified Aspergillus to section level in 56 of 59 specimens. With culture as the gold standard, our assay shows 100% sensitivity and specificity and constitutes an efficient alternative for identification of Aspergillus in lower respiratory tract samples.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Alonso M,Escribano P,Guinea J,Recio S,Simon A,Peláez T,Bouza E,García de Viedma Ddoi
10.1128/JCM.00176-12subject
Has Abstractpub_date
2012-10-01 00:00:00pages
3238-43issue
10eissn
0095-1137issn
1098-660Xpii
JCM.00176-12journal_volume
50pub_type
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