Abstract:
:Subgroup J avian leukosis virus (ALV-J) is an avian retrovirus that causes severe economic losses in the poultry industry. The early identification and removal of virus-shedding birds are important to reduce the spread of congenital and contact infections. In this study, a TaqMan-based real-time PCR method for the rapid detection and quantification of ALV-J with proviral DNA was developed. This method exhibited a high specificity for ALV-J. Moreover, the detection limit was as low as 10 viral DNA copies. The coefficients of variation (CVs) of both interassay and intra-assay reproducibility were less than 1%. The growth curves of ALV-J in DF-1 cells were measured by real-time PCR, yielding a trend line similar to those determined by 50% tissue culture infective dose (TCID(50)) and p27 antigen detection. Tissue samples suspected of ALV infection were evaluated using real-time PCR, virus isolation, and routine PCR, and the positivity rates were 60.1%, 41.6% and 44.5%, respectively. Our data indicated that the real-time PCR method provides a sensitive, specific, and reproducible diagnostic tool for the identification and quantification of ALV-J for clinical diagnosis and in laboratory research.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Qin L,Gao Y,Ni W,Sun M,Wang Y,Yin C,Qi X,Gao H,Wang Xdoi
10.1128/JCM.02030-12subject
Has Abstractpub_date
2013-01-01 00:00:00pages
149-54issue
1eissn
0095-1137issn
1098-660Xpii
JCM.02030-12journal_volume
51pub_type
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