Real-time reverse transcription-PCR assay for detection of mumps virus RNA in clinical specimens.

abstract：:Cryptococcus neoformans and Cryptococcus gattii are the main pathogenic species of invasive cryptococcosis among the Cryptococcus species. Taxonomic studies have shown that these two taxa have different genotypes or molecular types with biological and ecoepidemiological peculiarities. Matrix-assisted laser desorption ...

journal_title：Journal of clinical microbiology

authors： Siqueira LPM,Gimenes VMF,de Freitas RS,Melhem MSC,Bonfietti LX,da Silva AR Jr,Souza Santos LB,Motta AL,Rossi F,Benard G,de Almeida JN Jr

更新日期：2019-01-02 00:00:00

abstract：:An immunofluorescent-antibody test was developed for rapid detection of Pseudomonas aeruginosa in blood cultures. The test uses a murine monoclonal antibody specific for all strains of P. aeruginosa. In initial tests, bright uniform immunofluorescence signals were seen when each of the 17 international serotypes, as w...

journal_title：Journal of clinical microbiology

authors： Counts GW,Schwartz RW,Ulness BK,Hamilton DJ,Rosok MJ,Cunningham MD,Tam MR,Darveau RP

更新日期：1988-06-01 00:00:00

abstract：:The majority of existing human papillomavirus (HPV) genotyping assays are based on multiplex PCR using consensus or degenerate primers. We developed a Templex HPV assay that simultaneously detects and identifies 25 common HPV genotypes in a single-tube reaction using type-specific primers for the HPV-specific E6 and E...

journal_title：Journal of clinical microbiology

authors： Han J,Swan DC,Smith SJ,Lum SH,Sefers SE,Unger ER,Tang YW

更新日期：2006-11-01 00:00:00

abstract：:The control of vancomycin-resistant enterococci (VRE) has become an increasing burden on health care resources since their discovery over 20 years ago. Current techniques employed for their detection include time-consuming and laborious phenotypic methods or molecular methods requiring costly equipment and consumables...

journal_title：Journal of clinical microbiology

authors： Griffin PM,Price GR,Schooneveldt JM,Schlebusch S,Tilse MH,Urbanski T,Hamilton B,Venter D

更新日期：2012-09-01 00:00:00

abstract：:The long-chain fatty acids extracted from the whole cells of 12 clinically significant species of peptococci and peptostreptococci were characterized by gas-liquid chromatography. The resulting methylated fatty acid profiles (and some unidentified compounds) of 82 strains allowed the 12 species to be separated into fo...

journal_title：Journal of clinical microbiology

authors： Wells CL,Field CR

更新日期：1976-12-01 00:00:00

abstract：:The Leishmania species cause a variety of human disease syndromes. Methods for diagnosis and species differentiation are insensitive and many require invasive sampling. Although quantitative PCR (qPCR) methods are reported for leishmania detection, no systematic method to quantify parasites and determine the species i...

journal_title：Journal of clinical microbiology

authors： Weirather JL,Jeronimo SM,Gautam S,Sundar S,Kang M,Kurtz MA,Haque R,Schriefer A,Talhari S,Carvalho EM,Donelson JE,Wilson ME

更新日期：2011-11-01 00:00:00

abstract：:Lipopolysaccharide (LPS) of a reference strain of Yersinia enterocolitica serovar O15, Ye614, was examined for its chemical structure and antigenicity. The O15 LPS was partially hydrolyzed with diluted acetic acid and fractionated on a Sephadex G-50 column. Only one peak was detected at fractions where the R-core olig...

journal_title：Journal of clinical microbiology

authors： Kawaoka Y,Wauters G,Otsuki K,Tsubokura M

更新日期：1986-08-01 00:00:00

abstract：:Evaluation of optimal compositions of blood culture media has called for extensive and laborious work in comparative studies of large series of clincal specimens. Bacterial growth is accompanied by heat production, and calorimetry provides an analytical tool for its detection and quantification. A twin microcalorimete...

journal_title：Journal of clinical microbiology

authors： Ripa KT,Mårdh PA,Hovelius B,Ljungholm K

更新日期：1977-04-01 00:00:00

abstract：:Pasteurella multocida is a small nonmotile gram-negative coccobacillus that is found in the nasopharynx and gastrointestinal tract of many wild and domesticated animals. In humans it most commonly causes cellulitis and localized superficial skin abscesses following an animal bite or scratch. The respiratory tract is t...

journal_title：Journal of clinical microbiology

authors： Chen HI,Hulten K,Clarridge JE 3rd

更新日期：2002-09-01 00:00:00

abstract：:In specific-pathogen-free dogs experimentally infected with Borrelia burgdorferi by tick exposure, treatment with high doses of amoxicillin or doxycycline for 30 days diminished but failed to eliminate persistent infection. Although joint disease was prevented or cured in five of five amoxicillin- and five of six doxy...

journal_title：Journal of clinical microbiology

authors： Straubinger RK,Summers BA,Chang YF,Appel MJ

更新日期：1997-01-01 00:00:00

abstract：:A reverse transcriptase PCR (RT-PCR) was used for rapid determination of the hemagglutinin (HA) cleavage site sequence, a marker for the virulence potential of avian influenza viruses. When applied to specimens from chickens experimentally infected with either a virulent or an avirulent virus, RT-PCR uniformly detecte...

journal_title：Journal of clinical microbiology

authors： Horimoto T,Kawaoka Y

更新日期：1995-03-01 00:00:00

abstract：:A multiplexed biomarker bundle consisting of nine different inflammation markers was evaluated regarding their diagnostic and prognostic performances in 159 adult systemic inflammatory response syndrome (SIRS) patients enrolled at the emergency department. Fibronectin, interleukin-8 (IL-8), biotin, and neutrophil gela...

journal_title：Journal of clinical microbiology

authors： Reichsoellner M,Raggam RB,Wagner J,Krause R,Hoenigl M

更新日期：2014-11-01 00:00:00

abstract：:A total of 25 isolates of vanB-containing Enterococcus faecium were recovered from patients in a single Korean hospital over a 20-month period. There were two distinct vanB2 patterns among the 11 pulsed-field gel electrophoresis types; 17 contained the prototype vanB2 and 8 contained a novel vanB2 with a 177-bp deleti...

journal_title：Journal of clinical microbiology

authors： Lee WG,Jernigan JA,Rasheed JK,Anderson GJ,Tenover FC

更新日期：2001-03-01 00:00:00

abstract：:Many diseases with unknown etiology may be caused by unidentified viruses. Sequence-independent amplification revealed a new astrovirus, similar to VA1, in a 4-year-old male diagnosed with celiac disease. This expands the geographic range of this virus to include Europe and may associate astrovirus infection with the ...

journal_title：Journal of clinical microbiology

authors： Smits SL,van Leeuwen M,van der Eijk AA,Fraaij PL,Escher JC,Simon JH,Osterhaus AD

更新日期：2010-09-01 00:00:00

abstract：:An indirect immunofluorescence method was developed and used to detect urinary excretion of abnormal transitional cells infected with JC virus (JCV) or BK virus (BKV). This method was compared with urinary cytology, electron microscopy, viral culture, and viral serology in groups of immunosuppressed renal transplant r...

journal_title：Journal of clinical microbiology

authors： Hogan TF,Padgett BL,Walker DL,Borden EC,McBain JA

更新日期：1980-02-01 00:00:00

abstract：:Small round-structured viruses (SRSVs) or Norwalk-like viruses have been implicated as an important causative agent of gastroenteritis outbreaks. We used the relatively newly developed recombinant enzyme immunoassays (EIAs) to determine the seroprevalence of Norwalk virus (NV) and Mexico virus (MxV) in a family-based ...

journal_title：Journal of clinical microbiology

authors： Smit TK,Steele AD,Peenze I,Jiang X,Estes MK

更新日期：1997-09-01 00:00:00

abstract：:Sixty rotavirus-positive stool specimens from children with diarrhea were classified into G and P genotypes. G typing was done by PCR and then by hybridization with G type-specific (G1 to G4) oligonucleotide probes, whereas nested PCR was performed for P typing. Thirty-nine samples could be classified into both G and ...

journal_title：Journal of clinical microbiology

authors： Husain M,Seth P,Dar L,Broor S

更新日期：1996-06-01 00:00:00

abstract：:Combination therapy can successfully suppress human immunodeficiency virus (HIV) replication in patients but selects for drug resistance, requiring subsequent resistance-guided therapeutic changes. This report describes the development and validation of a novel assay that offers a uniform method to measure susceptibil...

journal_title：Journal of clinical microbiology

authors： Covens K,Dekeersmaeker N,Schrooten Y,Weber J,Schols D,Quiñones-Mateu ME,Vandamme AM,Van Laethem K

更新日期：2009-07-01 00:00:00

abstract：:Streptococcus mutans strains were isolated from cohorts of Brazilian nursery school children and genotyped by arbitrarily primed PCR and restriction fragment length polymorphism analysis. Of 24 children with two to five S. mutans isolates, 29% carried two or more genotypes. The presence of matching genotypes of S. mut...

journal_title：Journal of clinical microbiology

authors： Mattos-Graner RO,Li Y,Caufield PW,Duncan M,Smith DJ

更新日期：2001-06-01 00:00:00

abstract：:We attempted to find a relationship between the microbiological properties of bloodstream isolates of methicillin-resistant Staphylococcus aureus (MRSA) and the efficacy of vancomycin in the treatment of bacteremia. Vancomycin susceptibility testing was performed, and bactericidal activity was determined for 30 isolat...

journal_title：Journal of clinical microbiology

authors： Sakoulas G,Moise-Broder PA,Schentag J,Forrest A,Moellering RC Jr,Eliopoulos GM

更新日期：2004-06-01 00:00:00

abstract：:A micro enzyme-linked immunosorbent assay was used to detect the presence of amoebic antigen in polyethylene-glycol-precipitated circulating immune complexes (CIC). A cutoff dilution of greater than 1:160 for the precipitates was taken to be of clinical significance. Among the patients with amoebic liver abscess, 93% ...

journal_title：Journal of clinical microbiology

authors： Vinayak VK,Purnima,Singh K,Venkatwswarlu K,Nain CK,Mehta SK

更新日期：1986-06-01 00:00:00

abstract：:Seven commercial immunochromatographic assays intended for the detection of group A rotavirus antigens in human stool samples were evaluated. These assays showed similar levels of diagnostic accuracy and were suitable for the detection of rotavirus in patients with acute gastroenteritis but missed some asymptomatic ro...

journal_title：Journal of clinical microbiology

authors： Kaplon J,Fremy C,Pillet S,Mendes Martins L,Ambert-Balay K,Aho SL,Pothier P

更新日期：2015-11-01 00:00:00

abstract：:Blastomyces dermatitidis and Histoplasma capsulatum are dimorphic fungi that often cause self-limited respiratory infections. However, they may also cause severe disseminated disease, depending on the level of the exposure to the organism and the host immune status. In addition, patients with infections caused by thes...

journal_title：Journal of clinical microbiology

authors： Babady NE,Buckwalter SP,Hall L,Le Febre KM,Binnicker MJ,Wengenack NL

更新日期：2011-09-01 00:00:00

abstract：:Public health microbiology laboratories (PHLs) are on the cusp of unprecedented improvements in pathogen identification, antibiotic resistance detection, and outbreak investigation by using whole-genome sequencing (WGS). However, considerable challenges remain due to the lack of common standards. Here, we describe the...

journal_title：Journal of clinical microbiology

authors： Kozyreva VK,Truong CL,Greninger AL,Crandall J,Mukhopadhyay R,Chaturvedi V

更新日期：2017-08-01 00:00:00

abstract：:This report describes a DNA amplification procedure for routine identification of heat-labile-toxin-producing Escherichia coli. Two oligonucleotide primers were used in a polymerase chain reaction procedure to amplify a highly conserved region of the A subunit of the heat-labile enterotoxin gene. Amplifications were d...

journal_title：Journal of clinical microbiology

authors： Victor T,du Toit R,van Zyl J,Bester AJ,van Helden PD

更新日期：1991-01-01 00:00:00

abstract：:In recent studies, antigenic divergence has been observed in Bordetella pertussis circulating isolates. We collected 80 Bordetella pertussis isolates in Taiwan from 1998 to 2004 and analyzed them using a combination of pulsed-field gel electrophoresis (PFGE) and sequencing of the ptxS1 and prn genes. The incidence of ...

journal_title：Journal of clinical microbiology

authors： Yao SM,Lin YC,Chou CY,Chen YY,Hsiao MJ,Chen HY,Yan JJ,Su HP,Li SY

更新日期：2005-11-01 00:00:00

abstract：:The genetic diversity of 123 Streptococcus suis strains of capsular types 2, 1/2, 3, 7, and 9, isolated from pigs in France and from humans in different countries, was evaluated by pulsed-field gel electrophoresis (PFGE) of DNA restricted with SmaI. The method was highly discriminative (D = 0.98), results were reprodu...

journal_title：Journal of clinical microbiology

authors： Berthelot-Hérault F,Marois C,Gottschalk M,Kobisch M

更新日期：2002-02-01 00:00:00

abstract：:Metronidazole and tetracycline E tests were compared to an agar dilution method for the antimicrobial susceptibility testing of Helicobacter pylori. Sixteen strains were tested by using tetrazolium egg yolk (TEY) agar. The characteristic E test inhibition ellipse was clearer on TEY agar than on standard blood agar and...

journal_title：Journal of clinical microbiology

authors： Valdez Y,Velapatiño B,Gilman RH,Gutierrez V,León C

更新日期：1998-09-01 00:00:00

abstract：:The Analytab Products, Inc. (API), anaerobic multitest microsystem (MICRO) was compared with the Center for Disease Control conventional (CONV) thioglycolate (supplemented with hemin and vitamin K1) system and with pre-reduced anaerobically sterilized (PRAS) media as recommended by the Virginia Polytechnic Institute. ...

journal_title：Journal of clinical microbiology

authors： Moore HB,Sutter VL,Finegold SM

更新日期：1975-01-01 00:00:00

abstract：:The increasing risk of Rift Valley fever virus (RVFV) infection as a global veterinary and public health threat demands the development of safe and accurate diagnostic tests. The aim of this study was to assess the suitability of a baculovirus expression system to produce recombinant RVFV nucleoprotein (N) for use as ...

journal_title：Journal of clinical microbiology

authors： Faburay B,Wilson WC,Secka A,Drolet B,McVey DS,Richt JA

更新日期：2019-09-24 00:00:00