Abstract:
:In recent studies, antigenic divergence has been observed in Bordetella pertussis circulating isolates. We collected 80 Bordetella pertussis isolates in Taiwan from 1998 to 2004 and analyzed them using a combination of pulsed-field gel electrophoresis (PFGE) and sequencing of the ptxS1 and prn genes. The incidence of pertussis increases every 3 years, and most of the isolates prevalent since 1998 have expressed nonvaccine ptxS1A and prn2 alleles. Through PFGE analysis, all isolates could be classified into four major groups, and the incidence of these groups exhibited a correlation with the prn allele expressed by the isolates. We found that PFGE is more discriminative than gene sequencing, since it could divide the isolates expressing the prn2 allele into two groups: one group circulating from 1998 to 2001 and another group circulating from 2001 to 2004. The transition between the two groups in 2000 coincided with an outbreak of 326 cases. This research indicates that the antigenic divergence of B. pertussis circulating isolates has evolved over time in Taiwan. Such information will have implications for vaccine policy in Taiwan.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Yao SM,Lin YC,Chou CY,Chen YY,Hsiao MJ,Chen HY,Yan JJ,Su HP,Li SYdoi
10.1128/JCM.43.11.5457-5461.2005subject
Has Abstractpub_date
2005-11-01 00:00:00pages
5457-61issue
11eissn
0095-1137issn
1098-660Xpii
43/11/5457journal_volume
43pub_type
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