Indirect immunofluorescence assay for detection of Helicobacter pylori in human gastric mucosal biopsies.

abstract：:Whole-house surveillance for healthcare-associated infection is no longer the recommended practice because of the large personnel time investment required. We developed a computer-based tracking system using microbiologic data as an aid in detecting potential outbreaks of healthcare-associated infections on a hospital...

journal_title：Journal of clinical microbiology

authors： Hacek DM,Cordell RL,Noskin GA,Peterson LR

更新日期：2004-03-01 00:00:00

abstract：:Real-time PCR with melting curve analysis of PCR products is a rapid procedure for detecting and genotyping herpes simplex virus (HSV). When testing mucocutaneous samples for HSV by a real-time PCR assay targeting the DNA polymerase gene, we found that some PCR products had atypical melting curves that did not conform...

journal_title：Journal of clinical microbiology

authors： Anderson TP,Werno AM,Beynon KA,Murdoch DR

更新日期：2003-05-01 00:00:00

abstract：:We conducted a case-control study using the Fungitell assay, the novel Platelia Candida Antigen (Ag) Plus and Candida Antibody (Ab) Plus assays, and the Cand-Tec latex agglutination test to evaluate the usefulness of (1→3)-β-D-glucan (BDG), mannan antigen with/without anti-mannan antibody, and Cand-Tec Candida antigen...

journal_title：Journal of clinical microbiology

authors： Held J,Kohlberger I,Rappold E,Busse Grawitz A,Häcker G

更新日期：2013-04-01 00:00:00

abstract：:We compared examination of unstained and Gram-stained smears as methods of evaluating sputum quality. Of 100 slides examined, 96 (96%) were graded identically. Each discrepancy between methods was near the cutoff for specimen acceptability. We concluded that examination of unstained smears can provide an accurate, cos...

journal_title：Journal of clinical microbiology

authors： Wetterau LM,Zeimis RT,Hollick GE

更新日期：1986-07-01 00:00:00

abstract：:Evaluation of previously described chemically defined media for the growth of Legionella pneumophila showed that these media supported poor growth of several strains of L. pneumophila and did not support growth of certain of the Legionella species described later. Growth was stimulated by the dialysate from yeast extr...

journal_title：Journal of clinical microbiology

authors： Pine L,Franzus MJ,Malcolm GB

更新日期：1986-01-01 00:00:00

abstract：:We report the first documented case of endocarditis in a man infected with Bartonella alsatica, which causes bacteremia in healthy wild rabbits. B. alsatica was identified by serology and culture and by PCR of an aortic valve specimen. B. alsatica should be added to the list of zoonotic agents of blood culture-negativ...

journal_title：Journal of clinical microbiology

authors： Raoult D,Roblot F,Rolain JM,Besnier JM,Loulergue J,Bastides F,Choutet P

更新日期：2006-01-01 00:00:00

abstract：:Serologic methods are an important part of a clinical laboratory's portfolio of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) tests and are essential to the broader response to coronavirus infectious disease 2019 (COVID-19), including epidemiological studies and vaccine development. There are currently ...

journal_title：Journal of clinical microbiology

authors： Loeffelholz MJ

更新日期：2020-10-21 00:00:00

abstract：:Reference strains of serotypes 1 to 12 of Actinobacillus (Haemophilus) pleuropneumoniae were cultured in Eagle minimal essential medium with 10% Serum Plus. Culture supernatants were examined for cytotoxicity to alveolar macrophages and for the ability to hemolyze sheep erythrocytes. All strains except the reference s...

journal_title：Journal of clinical microbiology

authors： Kamp EM,van Leengoed LA

更新日期：1989-06-01 00:00:00

abstract：:We report the evaluation of recombinant severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) nucleocapsid protein enzyme-linked immunosorbent assay (ELISA)-based antibody tests for serodiagnosis of SARS-CoV pneumonia and compare the sensitivities and specificities of this ELISA for detection of immunoglobul...

journal_title：Journal of clinical microbiology

authors： Woo PC,Lau SK,Wong BH,Tsoi HW,Fung AM,Chan KH,Tam VK,Peiris JS,Yuen KY

更新日期：2004-05-01 00:00:00

abstract：:The standard complement fixation (CF) test, a commercial agarose gel immunodiffusion (AGID) test (ImmuCell Corporation, Portland, Maine), and two commercial enzyme-linked immunosorbent assays (ELISAs; Allied Laboratories, Glenwood Springs, Colo. [Allied ELISA], and the CSL, Limited, [Parkville, Victoria, Australia] en...

journal_title：Journal of clinical microbiology

authors： Sockett DC,Conrad TA,Thomas CB,Collins MT

更新日期：1992-05-01 00:00:00

abstract：:Norwalk virus (NV) and the Norwalk-like viruses are important human pathogens that cause epidemic acute viral gastroenteritis. Current techniques used to recover NV from clinical samples involve multistep viral extraction and elution procedures with subsequent viral detection by reverse transcription-PCR (RT-PCR). In ...

journal_title：Journal of clinical microbiology

authors： Schwab KJ,Estes MK,Neill FH,Atmar RL

更新日期：1997-02-01 00:00:00

abstract：:We report a case of septic arthritis of a native knee joint due to Corynebacterium striatum, a rare and unusual cause of septic arthritis of native joints. The isolate was identified by a combination of phenotypic, mass spectrometric, and nucleic acid-based assays and exhibited high-level resistance to most antimicrob...

journal_title：Journal of clinical microbiology

authors： Westblade LF,Shams F,Duong S,Tariq O,Bulbin A,Klirsfeld D,Zhen W,Sakaria S,Ford BA,Burnham CA,Ginocchio CC

更新日期：2014-05-01 00:00:00

abstract：:Macrorestriction profile analysis by pulsed-field gel electrophoresis (PFGE) was used to distinguish between seven isolates of Cowdria ruminantium from geographically different areas. Characteristic profiles were generated for each isolate by using the restriction endonucleases KspI, SalI, and SmaI with chromosomal si...

journal_title：Journal of clinical microbiology

authors： de Villiers EP,Brayton KA,Zweygarth E,Allsopp BA

更新日期：2000-05-01 00:00:00

abstract：:We performed pulsed-field gel electrophoresis on Escherichia coli O157 isolates (n = 318) from 199 healthy animals in a longitudinal study carried out on nine farms. Investigation of the restriction types proved that at the farm level, the same clones can be detected on sampling occasions separated by as much as 17 mo...

journal_title：Journal of clinical microbiology

authors： Liebana E,Smith RP,Batchelor M,McLaren I,Cassar C,Clifton-Hadley FA,Paiba GA

更新日期：2005-02-01 00:00:00

abstract：:A toxigenic Corynebacterium diphtheriae biovar mitis sequence type 136 (ST136) strain was recovered from a toe infection of an unvaccinated patient recently returned from India. The isolate was resistant to clindamycin, erythromycin (ermX positive), tetracycline, and trimethoprim-sulfamethoxazole, intermediate to ceft...

journal_title：Journal of clinical microbiology

authors： Mina NV,Burdz T,Wiebe D,Rai JS,Rahim T,Shing F,Hoang L,Bernard K

更新日期：2011-11-01 00:00:00

abstract：:The specificities and sensitivities of the Bactec mycobacterial growth indicator tube (MGIT) system for the recovery of Mycobacterium tuberculosis from pleural fluid are not statistically different than those of the Myco/F lytic liquid culture system. The time to positivity is shorter in the MGIT system (12.7 versus 2...

journal_title：Journal of clinical microbiology

authors： Harausz E,Lusiba JK,Nsereko M,Johnson JL,Toossi Z,Ogwang S,Boom WH,Joloba ML,Tuberculosis Research Unit (TBRU).

更新日期：2015-04-01 00:00:00

abstract：:The in vitro susceptibilities of 66 molecularly identified strains of the Mucorales to eight antifungals (amphotericin B, terbinafine, itraconazole, posaconazole, voriconazole, caspofungin, micafungin, and 5-fluorocytosine) were tested. Molecular phylogeny was reconstructed based on the nuclear ribosomal large subunit...

journal_title：Journal of clinical microbiology

authors： Vitale RG,de Hoog GS,Schwarz P,Dannaoui E,Deng S,Machouart M,Voigt K,van de Sande WW,Dolatabadi S,Meis JF,Walther G

更新日期：2012-01-01 00:00:00

abstract：:A total of 1,298 nonfermentative gram-negative rods were used to evaluate the performance of the individual biochemical tests in the N/F System and to determine whether the observed results included sufficient key reactions for rapid identification. The system included an oxidase test, two screening tubes providing 5 ...

journal_title：Journal of clinical microbiology

authors： Barnishan J,Ayers LW

更新日期：1979-02-01 00:00:00

abstract：:The epidemiology of 278 strains of Salmonella typhimurium isolated from 1973 to 1981 from animals in New York State was studied by using four "fingerprinting" techniques, bacteriophage type (B.R. Callow, J. Hyg. 57:346-359, 1959), biotype (J. P. Duguid, E. S. Anderson, G. A. Alfredsson, R. Barker, and D. C. Old, J. Me...

journal_title：Journal of clinical microbiology

authors： McDonough PL,Timoney JF,Jacobson RH,Khakhria R

更新日期：1989-04-01 00:00:00

abstract：:By DNA-DNA hybridization, we classified 26 human strains, 4 dog and cat strains, and 4 hamster strains putatively identified as Helicobacter cinaedi as well as 2 human strains and 2 animal strains of Helicobacter fennelliae. All but one human strain belonged to the same hybridization group as the type strain of H. cin...

journal_title：Journal of clinical microbiology

authors： Kiehlbauch JA,Brenner DJ,Cameron DN,Steigerwalt AG,Makowski JM,Baker CN,Patton CM,Wachsmuth IK

更新日期：1995-11-01 00:00:00

abstract：:We hypothesized that diversion of the first milliliter of venipuncture blood-the initial specimen diversion technique (ISDT)-would eliminate incompletely sterilized fragments of skin from the culture specimen and significantly reduce our blood culture contamination rate (R). We studied our hypothesis prospectively beg...

journal_title：Journal of clinical microbiology

authors： Patton RG,Schmitt T

更新日期：2010-12-01 00:00:00

abstract：:Pulsed-field gel electrophoresis (PFGE) typing was applied to the epidemiological investigation of 20 Serratia marcescens isolates collected from urine specimens of 17 patients and three urinals over a 2-month period. Twenty-five epidemiologically unrelated strains were also tested to determine the discriminatory powe...

journal_title：Journal of clinical microbiology

authors： Shi ZY,Liu PY,Lau YJ,Lin YH,HU BS

更新日期：1997-01-01 00:00:00

abstract：:A search has been made for corynebacterial phospholipase D, "ovis toxin," a sphingomyelinase (phosphatidylcholine phosphohydrolase, EC 3.1.4.4), among a wide variety of corynebacteria. Phospholipase D activity has been found in strains exhibiting the biochemical properties characteristic of Corynebacterium pseudotuber...

journal_title：Journal of clinical microbiology

authors： Barksdale L,Linder R,Sulea IT,Pollice M

更新日期：1981-02-01 00:00:00

abstract：:We compared the results of Gram staining and culture of cerebrospinal fluid to results obtained with a rapid PCR assay for the diagnosis of meningococcal meningitis in 281 cases of suspected bacterial meningitis. PCR had a sensitivity of 97% compared to a sensitivity of 55% for culture, and the PCR specificity was 99....

journal_title：Journal of clinical microbiology

authors： Richardson DC,Louie L,Louie M,Simor AE

更新日期：2003-08-01 00:00:00

abstract：:Seventeen Minnesota and Wisconsin dogs with granulocytic ehrlichosis were studied. The diagnoses were made by finding ehrlichia morulae in peripheral blood neutrophils. Eight dogs were studied retrospectively, and nine dogs were studied prospectively. The medical records of all dogs were reviewed. Eighty-eight percent...

journal_title：Journal of clinical microbiology

authors： Greig B,Asanovich KM,Armstrong PJ,Dumler JS

更新日期：1996-01-01 00:00:00

abstract：:The aim of this study was to determine if gastroenteric viruses were present on surfaces and equipment in a pediatric primary immunodeficiency unit (PPIU) by environmental sampling using swabs and subsequent nucleic acid extraction and reverse transcriptase PCR assays. A PPIU was chosen, and 11 swabs were taken at the...

journal_title：Journal of clinical microbiology

authors： Gallimore CI,Taylor C,Gennery AR,Cant AJ,Galloway A,Iturriza-Gomara M,Gray JJ

更新日期：2006-02-01 00:00:00

abstract：:In this study, the currently known typing methods for Mycobacterium tuberculosis isolates were evaluated with regard to reproducibility, discrimination, and specificity. Therefore, 90 M. tuberculosis complex strains, originating from 38 countries, were tested in five restriction fragment length polymorphism (RFLP) typ...

journal_title：Journal of clinical microbiology

authors： Kremer K,van Soolingen D,Frothingham R,Haas WH,Hermans PW,Martín C,Palittapongarnpim P,Plikaytis BB,Riley LW,Yakrus MA,Musser JM,van Embden JD

更新日期：1999-08-01 00:00:00

abstract：:Recent studies have shown the public health importance of identifying individuals with acute human immunodeficiency virus infection (AHI); however, the cost of nucleic acid amplification testing (NAAT) makes individual testing of at-risk individuals prohibitively expensive in many settings. Pooled NAAT (or group testi...

journal_title：Journal of clinical microbiology

authors： Westreich DJ,Hudgens MG,Fiscus SA,Pilcher CD

更新日期：2008-05-01 00:00:00

abstract：:A simplified procedure for serotyping Campylobacter jejuni and Campylobacter coli on the basis of thermostable antigens was developed and tested for its applicability as a routine typing method. The assay involves the sensitization of erythrocytes with an antigenic extract and performance of a slide agglutination assa...

journal_title：Journal of clinical microbiology

authors： Mills SD,Congi RV,Hennessy JN,Penner JL

更新日期：1991-10-01 00:00:00

abstract：:Sepsis is a major cause of death worldwide among nonhospitalized people and hospitalized patients. A wide range of pathogens are involved, and the correct identification and correct antimicrobial therapy are critical to ensure optimal clinical outcomes. With the recent introduction of matrix-assisted laser desorption ...

journal_title：Journal of clinical microbiology

authors： Foster AG

更新日期：2013-11-01 00:00:00