Macrorestriction fragment profiles reveal genetic variation of Cowdria ruminantium isolates.

abstract：:Sequence detection by the 5' nuclease TaqMan assay uses online detection of internal fluorogenic probes in closed PCR tubes. Primers and probe were chosen from a part of the omlA gene common to all serotypes of Actinobacillus pleuropneumoniae, which gave an amplicon of 92 bp. The test was evaluated with 73 lung isolat...

journal_title：Journal of clinical microbiology

authors： Angen O,Jensen J,Lavritsen DT

更新日期：2001-01-01 00:00:00

abstract：:A multilaboratory study designed to determine the in vitro susceptibility criteria and quality control parameters for ofloxacin against Neisseria gonorrhoeae was conducted according to the guidelines of the National Committee for Clinical Laboratory Standards. Proposed susceptibility breakpoints are MICs of less than ...

journal_title：Journal of clinical microbiology

authors： Fuchs PC,Barry AL,Baker C,Murray PR,Washington JA

更新日期：1992-04-01 00:00:00

abstract：:mRNAs of human papillomaviruses (HPV) 16 and 18 were detected in cancer-derived cell lines and genital tract biopsy specimens by a novel hybridization assay. Biotinylated whole genomic HPV DNA probes were hybridized in solution to extracted total nucleic acids. Hybrids between the labeled probes and RNA transcripts we...

journal_title：Journal of clinical microbiology

authors： Coutlée F,Shah KV,Rader JS,Currie JL,Viscidi RP

更新日期：1991-05-01 00:00:00

abstract：:Strains of Candida albicans obtained from human immunodeficiency virus (HIV)-positive individuals prior to their first episode of oral thrush were already in a high-frequency mode of switching and were far more resistant to a number of antifungal drugs than commensal isolates from healthy individuals. Switching in the...

journal_title：Journal of clinical microbiology

authors： Vargas K,Messer SA,Pfaller M,Lockhart SR,Stapleton JT,Hellstein J,Soll DR

更新日期：2000-10-01 00:00:00

abstract：:Potomac horse fever, a recently recognized disease of equines, characterized by high fever, leukopenia, and a profuse diarrhea, was studied for its etiology. An Ehrlichia organism was isolated in equine macrophage-fibroblast cell cultures and mouse macrophage cell cultures from the mononuclear cells of blood of infect...

journal_title：Journal of clinical microbiology

authors： Dutta SK,Myrup AC,Rice RM,Robl MG,Hammond RC

更新日期：1985-08-01 00:00:00

abstract：:The ERA strain of rabies virus was adapted to growth on monolayers of a porcine kidney cell line (PK-2A). A reproducible plaque assay system was subsequently developed, which appears to be satisfactory for conducting plaque neutralization tests. ...

journal_title：Journal of clinical microbiology

authors： Strating A,Mitzel JR,Slater EA

更新日期：1976-08-01 00:00:00

abstract：:More than 4,000 clinical urine specimens were evaluated with an automated microbial detection/identification system compared to a standarized manual analysis and the routine modalities used in five peer-group laboratories. The comparison indicates that the automated system recognizes the nine groups of significant mic...

journal_title：Journal of clinical microbiology

authors： Isenberg HD,Gavan TL,Sonnenwirth A,Taylor WI,Washington JA 2nd

更新日期：1979-08-01 00:00:00

abstract：:We present a case of invasive pneumococcal infection in a healthy 10-month-old infant from whom Streptococcus pneumoniae serotype 23F was isolated from the blood and serotype 23B was isolated from the cerebrospinal fluid. Both serotypes were penicillin nonsusceptible. Pulsed-field gel electrophoresis analysis demonstr...

journal_title：Journal of clinical microbiology

authors： de Andrade AL,Pimenta FC,Laval CA,de Andrade JG,Guerra ML,Brandileone MC

更新日期：2004-05-01 00:00:00

abstract：:This study reports the first isolation of Candida dubliniensis from North American Indians. Of 39 healthy human hosts sampled, two had C. dubliniensis. Genotypic analysis identified polymorphisms in these strains and differences from two reference strains. Our results suggest that yeast populations from indigenous com...

journal_title：Journal of clinical microbiology

authors： Montour L,Tey R,Xu J

更新日期：2003-07-01 00:00:00

abstract：:Sheep and guinea pigs were immunized with cellular and extracellular antigen from Legionella pneumophila bacteria. After immunization, the animals developed immunoglobulin G titers against the immunizing agent. The same sera were also tested in a Mycoplasma pneumoniae complement fixation test. All the preimmunization ...

journal_title：Journal of clinical microbiology

authors： Berdal BP,Eng J

更新日期：1982-11-01 00:00:00

abstract：:Nontuberculous mycobacterial lymphadenitis presents an increasing clinical problem in immunocompetent young children. A slowly growing, nonphotochromogenic mycobacterium was recovered twice (isolates 2553/91 and 2554/91) from the lymphatic tissue of a child with recurrent cervical lymphadenitis. It could be differenti...

journal_title：Journal of clinical microbiology

authors： Haas WH,Butler WR,Kirschner P,Plikaytis BB,Coyle MB,Amthor B,Steigerwalt AG,Brenner DJ,Salfinger M,Crawford JT,Böttger EC,Bremer HJ

更新日期：1997-12-01 00:00:00

abstract：:Increasing diversity among influenza H5N1 viruses has resulted in the need for sensitive and specific diagnostic assays, fully validated for the detection of H5 viruses belonging to all hemagglutinin (HA) clades, particularly the recently circulating H5N1 viruses of clade 2. In this report, the development and validat...

journal_title：Journal of clinical microbiology

authors： Ellis JS,Smith JW,Braham S,Lock M,Barlow K,Zambon MC

更新日期：2007-05-01 00:00:00

abstract：:The NeutRELISA, a modification of the receptor enzyme-linked immunosorbent assay developed for the detection of verocytotoxin 1 (VT1) which permits the rapid detection of neutralizing antibodies (NAb) against this toxin, has been performed. A standard concentration of VT1 was preincubated with VT-immune or nonimmune r...

journal_title：Journal of clinical microbiology

authors： Boulanger J,Petric M,Lingwood C,Law H,Roscoe M,Karmali M

更新日期：1990-12-01 00:00:00

abstract：:A total of 233 isolates of Pasteurella multocida were obtained from 2,912 cases of clinical respiratory disease in pigs in China, giving an isolation rate of 8.0%. Serogroup A P. multocida isolates were isolated from 92 cases (39.5%), and serogroup D isolates were isolated from 128 cases (54.9%); 12 isolates (5.2%) we...

journal_title：Journal of clinical microbiology

authors： Tang X,Zhao Z,Hu J,Wu B,Cai X,He Q,Chen H

更新日期：2009-04-01 00:00:00

abstract：:Previously, we designed an internally controlled quantitative nested real-time (QNRT) PCR assay for Mycobacterium tuberculosis DNA in order to rapidly diagnose tuberculous meningitis. This technique combined the high sensitivity of nested PCR with the accurate quantification of real-time PCR. In this study, we attempt...

journal_title：Journal of clinical microbiology

authors： Takahashi T,Tamura M,Asami Y,Kitamura E,Saito K,Suzuki T,Takahashi SN,Matsumoto K,Sawada S,Yokoyama E,Takasu T

更新日期：2008-05-01 00:00:00

abstract：:Syrian hamsters were shown to be susceptible to infection by the Lyme disease spirochete Borrelia burgdorferi. Although these spirochetes did not cause any outward signs of illness in these animals, they did cause a generalized infection. Spirochetemia was present during the first 6 days of infection. At 14 days posti...

journal_title：Journal of clinical microbiology

authors： Johnson RC,Marek N,Kodner C

更新日期：1984-12-01 00:00:00

abstract：:Chronic periodontitis is a common infectious disease in the adult population. The etiology is clearly bacterial, and a small number of bacterial species have been consistently associated with periodontitis, including Bacteroides forsythus and Porphyromonas gingivalis. Comparatively little attention has been paid to th...

journal_title：Journal of clinical microbiology

authors： Leys EJ,Lyons SR,Moeschberger ML,Rumpf RW,Griffen AL

更新日期：2002-03-01 00:00:00

abstract：:We cloned and characterized a phosphatidylcholine-hydrolyzing phospholipase C (PC-PLC) gene from Burkholderia pseudomallei. DNA sequence analysis of the gene indicated an open reading frame coding for 700 amino acids with a 34-amino-acid signal peptide. When cleaved, this yields a secreted 73-kDa mature protein. The d...

journal_title：Journal of clinical microbiology

authors： Korbsrisate S,Suwanasai N,Leelaporn A,Ezaki T,Kawamura Y,Sarasombath S

更新日期：1999-11-01 00:00:00

abstract：:A total of 53 strains of enterococci, including recently isolated strains with high-level resistance to vancomycin, were tested for vancomycin susceptibility by broth microdilution and disk diffusion using Mueller-Hinton media with and without supplementation with 5% blood. By using currently published parameters of t...

journal_title：Journal of clinical microbiology

authors： Swenson JM,Hill BC,Thornsberry C

更新日期：1989-09-01 00:00:00

abstract：:Real-time quantitative PCR (qPCR) assay of sputum or nasopharyngeal specimens has shown promising results in the detection of pneumococcal community-acquired pneumonia (PncCAP). We applied qPCR for the autolysin gene (lytA) and compared sputum and nasopharyngeal swab (NPS) pneumococcal loads in elderly patients with c...

journal_title：Journal of clinical microbiology

authors： Saukkoriipi A,Palmu AA,Pascal T,Verlant V,Hausdorff WP,Jokinen J

更新日期：2017-12-26 00:00:00

abstract：:HIV-1 drug resistance (HIVDR) assays are important tools in clinical management of HIV-infected patients on antiretroviral therapy (ART) and surveillance of drug-resistant variants at population levels. The high cost associated with commercial assays hinders their use in resource-limited settings. We adopted and valid...

journal_title：Journal of clinical microbiology

authors： Inzaule S,Yang C,Kasembeli A,Nafisa L,Okonji J,Oyaro B,Lando R,Mills LA,Laserson K,Thomas T,Nkengasong J,Zeh C

更新日期：2013-02-01 00:00:00

abstract：:The genotypes of 63 isolates of Aspergillus fumigatus obtained from three hospitals in different geographical areas and of eight culture collection strains were determined by multilocus enzyme electrophoresis. Twelve of the 17 enzymatic loci studied were polymorphic, giving rise to 48 different electrophoretic types. ...

journal_title：Journal of clinical microbiology

authors： Rodriguez E,De Meeüs T,Mallie M,Renaud F,Symoens F,Mondon P,Piens MA,Lebeau B,Viviani MA,Grillot R,Nolard N,Chapuis F,Tortorano AM,Bastide JM

更新日期：1996-10-01 00:00:00

abstract：:Human immunodeficiency virus type 1 serotype C was found in 545 of 712 Ethiopian patients by peptide enzyme immunoassay. Serotyping failed in 146 samples due to the absence of V3 antibodies or multiple reactivities. In 6 of 34 samples, discordant results were obtained by serotyping and genotyping, possibly due to dive...

journal_title：Journal of clinical microbiology

authors： Sherefa K,Sällberg M,Johansson B,Salminen M,Sönnerborg A

更新日期：1997-09-01 00:00:00

abstract：:Cellulosimicrobium funkei is a rare, opportunistic pathogen. We describe a case of bacteremia and possibly prosthetic valve endocarditis by this organism in a nonimmunocompromised patient. Useful phenotypic tests for differentiating C. funkei from Cellulosimicrobium cellulans and Cellulosimicrobium terreum include mot...

journal_title：Journal of clinical microbiology

authors： Petkar H,Li A,Bunce N,Duffy K,Malnick H,Shah JJ

更新日期：2011-03-01 00:00:00

abstract：:Tissue cultures established from brain tissues of six patients with various chronic, degenerative diseases did not show evidence of viral agents. Sucrose gradient analysis of radioactively labeled culture fluid from two patients failed to reveal the presence of any incomplete or defective virus. Extracellular fluids f...

journal_title：Journal of clinical microbiology

authors： Lennette ET

更新日期：1975-05-01 00:00:00

abstract：:Inactivation of penicillin and gentamicin in cultures was achieved by using monoclonal antibodies against these antibiotics. A viridans group streptococcus (penicillin MIC, less than or equal to 0.06 micrograms/ml) and Escherichia coli ATCC 35218 (gentamicin MIC, less than or equal to 1 microgram/ml) were able to grow...

journal_title：Journal of clinical microbiology

authors： Sierra-Madero JG,Caulfield MJ,Hall GS,Washington JA

更新日期：1988-09-01 00:00:00

abstract：:Statistical analysis of a limiting dilution assay (LDA) showed that the occurrence of infectious human immunodeficiency virus type 1 (HIV-1)-harboring cells in serially diluted samples of peripheral blood mononuclear cells (PBMCs) of HIV-1-seropositive patients fits the model describing a single-hit Poisson distributi...

journal_title：Journal of clinical microbiology

authors： Lu W,Manolikakis G,Andrieu JM

更新日期：1992-10-01 00:00:00

abstract：:Trauma intensive care unit (TICU) patients requiring mechanical respiratory support frequently develop ventilator-associated pneumonia (VAP). Oral and oropharyngeal bacteria are believed to be responsible for many cases of VAP, but definitive evidence of this relationship is lacking. Earlier studies used conventional ...

journal_title：Journal of clinical microbiology

authors： Bahrani-Mougeot FK,Paster BJ,Coleman S,Barbuto S,Brennan MT,Noll J,Kennedy T,Fox PC,Lockhart PB

更新日期：2007-05-01 00:00:00

abstract：:Several studies have identified nonsterile blood collection tubes as the reservoir for contaminating microorganisms in outbreaks of pseudobacteremia. Microorganisms are thought to be transferred from the nonsterile tubes to the blood culture tubes when blood is drawn for cultures, but the validity of this hypothesis h...

journal_title：Journal of clinical microbiology

authors： McNeil MM,Davis BJ,Anderson RL,Martone WJ,Solomon SL

更新日期：1985-07-01 00:00:00

abstract：:A new PCR-reverse dot blot hybridization (RDBH) assay was developed for the rapid identification of Mycobacterium species in clinical isolates. The assay, which targets the 16S rRNA, was evaluated for 27 mycobacterial reference strains and 340 clinical isolates that were simultaneously identified by DNA sequencing and...

journal_title：Journal of clinical microbiology

authors： Wu X,Zhang J,Liang J,Lu Y,Li H,Li C,Yue J,Zhang L,Liu Z

更新日期：2007-06-01 00:00:00