Abstract:
:The NeutRELISA, a modification of the receptor enzyme-linked immunosorbent assay developed for the detection of verocytotoxin 1 (VT1) which permits the rapid detection of neutralizing antibodies (NAb) against this toxin, has been performed. A standard concentration of VT1 was preincubated with VT-immune or nonimmune rabbit serum. The serum-toxin mixtures were then added to microtiter plates coated with deacylated globotriosyl ceramide (lyso-Gb3). The reduction of VT1 binding to lyso-Gb3 in the immune serum-toxin mixtures compared with the VT1-Gb3 binding in the nonimmune serum-toxin mixtures was detected by using mouse monoclonal antibody to VT1. After standardization of the NeutRELISA with rabbit sera, 57 human control serum samples were tested to establish a cutoff value below which NeutRELISA results would be considered positive. Thirty-three single serum samples known to demonstrate NAb to VT1 by biological assay reproducibly demonstrated VT1 NAb when tested by the NeutRELISA. There was a close correlation between the biological VT1 neutralization assay and the NeutRELISA. This assay offers a practical, rapid, and reliable approach for the detection of NAb to VT1 and other verocytotoxins.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Boulanger J,Petric M,Lingwood C,Law H,Roscoe M,Karmali Mdoi
10.1128/JCM.28.12.2830-2833.1990subject
Has Abstractpub_date
1990-12-01 00:00:00pages
2830-3issue
12eissn
0095-1137issn
1098-660Xjournal_volume
28pub_type
杂志文章abstract::An assay to detect and sequence DNA from human cytomegalovirus (HCMV) immediate-early gene region 1 has been developed; it involves in vitro amplification by the polymerase chain reaction and direct solid-phase sequencing of the amplified material. Urine samples from 16 patients tested positive for HCMV DNA in both a ...
journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.26.8.1598-1599.1988
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.20.6.1099-1101.1984
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doi:10.1128/JCM.34.11.2766-2769.1996
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.26.2.380-381.1988
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.33.3.576-580.1995
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.36.6.1518-1529.1998
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.22.5.757-760.1985
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journal_title:Journal of clinical microbiology
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abstract::Rapidly growing mycobacteria are rarely found in central nervous system infections. We describe a case of polymicrobial infection in a brain abscess including two rapidly growing Mycobacterium species, M. immunogenum and M. llatzerense. The Mycobacterium isolates were distinguishable by molecular methods, and whole-ge...
journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
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abstract::The one-step single-tube betaine-free reverse transcription (RT)-loop-mediated isothermal amplification assay was developed for rapid diagnosis of hepatitis E virus. This assay amplified the target gene in less than 45 min (even as short as 20 min) under isothermal conditions at 63 degrees C, and the sensitivity of th...
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