Novel IS711-specific chromosomal locations useful for identification and classification of marine mammal Brucella strains.

abstract：:Knowledge of polyomavirus BK (BKV) genomic diversity has greatly expanded. The implications of BKV DNA sequence variation for the performance of molecular diagnostic assays is not well studied. We analyzed 184 publically available VP-1 sequences encompassing the BKV genomic region targeted by an in-house quantitative ...

journal_title：Journal of clinical microbiology

authors： Randhawa P,Kant J,Shapiro R,Tan H,Basu A,Luo C

更新日期：2011-12-01 00:00:00

abstract：:Nosocomial bronchopneumonia is a frequent complication in patients with chronic intratracheal intubation. Despite targeted antibiotic treatment, production of abundant bronchial secretion containing pathogen bacteria often tends to be chronic, and so mortality drastically increases. This problem led to an investigatio...

journal_title：Journal of clinical microbiology

authors： Klekner A,Bagyi K,Bognar L,Gaspar A,Andrasi M,Szabo J

更新日期：2006-09-01 00:00:00

abstract：:Aspirates of liver abscesses were analyzed for Entamoeba histolytica. PCR detected a gene encoding a 30-kDa protein in all samples but detected the ribosomal DNA gene in only 14 (33.3%) samples. Enzyme-linked immunosorbent assay detected antigen in 41 (97.6%) samples. PCR analysis of a strain-specific antigen (SSG) re...

journal_title：Journal of clinical microbiology

authors： Zengzhu G,Bracha R,Nuchamowitz Y,Cheng-I W,Mirelman D

更新日期：1999-09-01 00:00:00

abstract：:Seven commercial immunochromatographic assays intended for the detection of group A rotavirus antigens in human stool samples were evaluated. These assays showed similar levels of diagnostic accuracy and were suitable for the detection of rotavirus in patients with acute gastroenteritis but missed some asymptomatic ro...

journal_title：Journal of clinical microbiology

authors： Kaplon J,Fremy C,Pillet S,Mendes Martins L,Ambert-Balay K,Aho SL,Pothier P

更新日期：2015-11-01 00:00:00

abstract：:High-risk human papillomavirus (hrHPV) testing is now being introduced as a potential primary screening test for improved detection of cervical precancer and cancer. Current U.S. Food and Drug Administration-approved tests are batch tests that take several hours to complete. A rapid, non-batch test might permit point-...

journal_title：Journal of clinical microbiology

authors： Einstein MH,Smith KM,Davis TE,Schmeler KM,Ferris DG,Savage AH,Gray JE,Stoler MH,Wright TC Jr,Ferenczy A,Castle PE

更新日期：2014-06-01 00:00:00

abstract：:A rapid method for presumptive identification of black-pigmented gram-negative anaerobic rods was developed. Using filter paper spot tests for indole production, sialidase, alpha-glucosidase, beta-glucosidase, alpha-fucosidase, and trypsinlike enzyme activities, 100% of Porphyromonas gingivalis, Prevotella intermedia,...

journal_title：Journal of clinical microbiology

authors： Moncla BJ,Braham P,Rabe LK,Hillier SL

更新日期：1991-09-01 00:00:00

abstract：:We developed a quick genetic approach to screen variants of the intimin gene (eae) by using a heteroduplex mobility assay (HMA) that targets the 5' conserved region of eae. The eae variants were categorized into 4 major HMA types and 10 minor subtypes. ...

journal_title：Journal of clinical microbiology

authors： Ito K,Iida M,Yamazaki M,Moriya K,Moroishi S,Yatsuyanagi J,Kurazono T,Hiruta N,Ratchtrachenchai OA

更新日期：2007-03-01 00:00:00

abstract：:The CLSI (formerly NCCLS) M38-A document for antifungal susceptibility testing of filamentous fungi does not describe guidelines for echinocandins. A multicenter study (eight centers) evaluated inter- and intralaboratory reproducibilities of two reading times (24 and 48 h or 48 and 72 h) and two end points (MICs and m...

journal_title：Journal of clinical microbiology

authors： Espinel-Ingroff A,Fothergill A,Ghannoum M,Manavathu E,Ostrosky-Zeichner L,Pfaller MA,Rinaldi MG,Schell W,Walsh TJ

更新日期：2007-07-01 00:00:00

abstract：:Of 45 influenza virus strains (43 type A and 2 type B) detected in conventional tube cell cultures (average time, 4 days), 25 (56%) were detected by immunofluorescence in the shell vial assay 24 h postinoculation. The specific fluorescence produced should allow this procedure to be readily adapted by laboratories with...

journal_title：Journal of clinical microbiology

authors： Espy MJ,Smith TF,Harmon MW,Kendal AP

更新日期：1986-10-01 00:00:00

abstract：:A prospective study was performed comparing the fluorescent antibody to membrane antigen (FAMA) test and the enzyme-linked immunosorbent assay (ELISA) for identifying susceptibility and seroconversion to varicella-zoster virus (VZV) infection. A total of 75 sera were collected from index cases and from sibling and par...

journal_title：Journal of clinical microbiology

authors： Iltis JP,Castellano GA,Gerber P,Le C,Vujcic LK,Quinnan GV Jr

更新日期：1982-11-01 00:00:00

abstract：:Fosfomycin has been shown to have a wide spectrum of activity against multidrug-resistant Gram-negative bacteria; however, breakpoints have been established only for Escherichia coli or Enterobacterales per the Clinical and Laboratory Standards Institute (CLSI) and the European Committee on Antimicrobial Susceptibilit...

journal_title：Journal of clinical microbiology

authors： Smith EC,Brigman HV,Anderson JC,Emery CL,Bias TE,Bergen PJ,Landersdorfer CB,Hirsch EB

更新日期：2020-09-22 00:00:00

abstract：:The AutoMicrobic system (AMS) is capable of identifying most Enterobacteriaceae within 8 h and many glucose-nonfermenting, gram-negative bacilli after 13 h of incubation. Early preliminary results can be readily obtained from the computer as the tests incubate. Data with 1,023 bacterial isolates were reviewed to deter...

journal_title：Journal of clinical microbiology

authors： Barry AL,Badal RE

更新日期：1982-08-01 00:00:00

abstract：:The in vitro susceptibilities of 66 molecularly identified strains of the Mucorales to eight antifungals (amphotericin B, terbinafine, itraconazole, posaconazole, voriconazole, caspofungin, micafungin, and 5-fluorocytosine) were tested. Molecular phylogeny was reconstructed based on the nuclear ribosomal large subunit...

journal_title：Journal of clinical microbiology

authors： Vitale RG,de Hoog GS,Schwarz P,Dannaoui E,Deng S,Machouart M,Voigt K,van de Sande WW,Dolatabadi S,Meis JF,Walther G

更新日期：2012-01-01 00:00:00

abstract：:The biochemical characteristics and the presence of human Fc(gamma) receptors of 52 throat isolates of group C beta-hemolytic streptococci were examined. Among these isolates, 38 were identified as "Streptococcus milleri" and 14 were identified as "Streptococcus equisimilis." The differentiation of group C "S. equisim...

journal_title：Journal of clinical microbiology

authors： Lebrun L,Guibert M,Wallet P,de Maneville MM,Pillot J

更新日期：1986-11-01 00:00:00

abstract：:Prosthetic joint infection (PJI) is a rare but refractory complication of arthroplasty. Accurate identification of pathogens is a key step for successful treatment of PJI, which remains a challenge for clinicians and laboratory workers. We designed a combined culture method with sonication of implants and incubation i...

journal_title：Journal of clinical microbiology

authors： Shen H,Tang J,Wang Q,Jiang Y,Zhang X

更新日期：2015-03-01 00:00:00

abstract：:Hybridomas producing monoclonal antibodies to VP7, a major neutralizing protein of serotype 9 rotavirus (strain W161), were prepared. One monoclonal antibody, W161-6A1, was shown to neutralize only serotype 9 rotavirus strains and reacted specifically with serotype 9 rotaviruses in an enzyme-linked immunosorbent assay...

journal_title：Journal of clinical microbiology

authors： Midthun K,Valdesuso J,Kapikian AZ,Hoshino Y,Green KY

更新日期：1989-09-01 00:00:00

abstract：:Streptococcus pyogenes, a major human pathogen, is still considered susceptible to beta-lactams, but for other relevant antibiotics, highly variable resistance rates have been reported. Since no data were available from Iran, we tested 1,335 throat isolates from two different regions of the country for their antibioti...

journal_title：Journal of clinical microbiology

authors： Jasir A,Tanna A,Noorani A,Mirsalehian A,Efstratiou A,Schalen C

更新日期：2000-06-01 00:00:00

abstract：:We developed a group B streptococcus multiplex PCR assay which allows, by direct analysis of the amplicon size, determination of the surface protein antigen genes of alpha-C protein, epsilon protein, Rib, Alp2, Alp3, and Alp4. The multiplex PCR assay offers a rapid and simple method of subtyping Streptococcus agalacti...

journal_title：Journal of clinical microbiology

authors： Creti R,Fabretti F,Orefici G,von Hunolstein C

更新日期：2004-03-01 00:00:00

abstract：:An evaluation of the serotyping system for Proteus rettgeri has been performed through its application to 561 human isolates of this species. Of these, 545 were typable and could be separated into 58 O serotypes. A group of isolates from one hospital, and clinically implicated in cross-infections, was found to belong ...

journal_title：Journal of clinical microbiology

authors： Penner JL,Hinton NA,Hennessy JN

更新日期：1976-04-01 00:00:00

abstract：:Tick-borne ehrlichial pathogens of animals and humans require a mammalian reservoir of infection from which ticks acquire the organism for subsequent transmission. In the present study, we examined the strain structure of Anaplasma marginale, a genogroup II ehrlichial pathogen, in both an acute outbreak and in persist...

journal_title：Journal of clinical microbiology

authors： Palmer GH,Rurangirwa FR,McElwain TF

更新日期：2001-02-01 00:00:00

abstract：:Feces and serum specimens were collected from three farms in Michigan on which approximately 50-lb (8- to 9-week-old) pigs experienced diarrhea just after placement into all-in-all-out finishing barns. The clinical signs (profuse watery diarrhea lasting about 2 weeks and no vomiting) were similar on all farms, and the...

journal_title：Journal of clinical microbiology

authors： Kim Y,Chang KO,Straw B,Saif LJ

更新日期：1999-05-01 00:00:00

abstract：:We studied nine solid and two liquid media for their suitability to select Aeromonas and Plesiomonas spp. from human stools, using artificially contaminated samples as well as 254 samples from outpatients with and without diarrhea. Media with optimal sensitivity and specificity for Aeromonas spp. were alkaline peptone...

journal_title：Journal of clinical microbiology

authors： von Graevenitz A,Bucher C

更新日期：1983-01-01 00:00:00

abstract：:Adenoviruses can be demonstrated readily in the stools of pediatric gastroenteritis patients by electron microscopy or counterimmunoelectrophoresis, but in 45% of these cases the virus will not grow in cell culture. Indirect immunofluorescence microscopy can be used to detect nongrowing strains of adenovirus; these st...

journal_title：Journal of clinical microbiology

authors： Retter M,Middleton PJ,Tam JS,Petric M

更新日期：1979-10-01 00:00:00

abstract：:A genomics-based PCR method was developed and used to test specimens from patients involved in a large outbreak of Mycoplasma pneumoniae in a closed religious community in New York State. New P1 adhesin gene primers were designed to bind to 9 of 10 target sequences in the repetitive-element sequences obtained from the...

journal_title：Journal of clinical microbiology

authors： Waring AL,Halse TA,Csiza CK,Carlyn CJ,Arruda Musser K,Limberger RJ

更新日期：2001-04-01 00:00:00

abstract：:The performance of four media for the isolation of Aeromonas strains from stool specimens, the importance of ampicillin-susceptible Aeromonas strains in the selection of culture media, and the usefulness of beta-hemolysis in screening blood-containing media for Aeromonas strains were evaluated in two phases. In the fi...

journal_title：Journal of clinical microbiology

authors： Kelly MT,Stroh EM,Jessop J

更新日期：1988-09-01 00:00:00

abstract：:We report a nonfatal case of streptococcal toxic shock syndrome (STSS) caused by a Streptococcus pyogenes emm118 strain encoding a novel variant of streptococcal mitogenic exotoxin Z (SMEZ-34). This variant was responsible for the major mitogenic activity in the cell culture supernatant. Patient sera showed seroconver...

journal_title：Journal of clinical microbiology

authors： Yang L,Thomas M,Woodhouse A,Martin D,Fraser JD,Proft T

更新日期：2005-07-01 00:00:00

abstract：:Immune pressure on lentiviruses exerted by cytotoxic T lymphocytes (CTL) selects for virus CTL epitope mutations. Currently employed methods for monitoring emerging CTL epitope mutations rely on the labor-intensive and time-consuming techniques of virus population or clonal sequencing. Here we describe the development...

journal_title：Journal of clinical microbiology

authors： Peyerl FW,Barouch DH,Bazick HS,Manuel E,Letvin NL

更新日期：2005-09-01 00:00:00

abstract：:The genomes of two novel human papillomavirus (HPV) types, HPV68 and HPV70, were cloned from a low-grade cervical intraepithelial neoplasia and a vulvar papilloma, respectively, and partially sequenced. Both types are related to HPV39, a potentially oncogenic virus. HPV68 and HPV70 were also detected in genital intrae...

journal_title：Journal of clinical microbiology

authors： Longuet M,Beaudenon S,Orth G

更新日期：1996-03-01 00:00:00

abstract：:Nasopharyngeal secretions from adults and children were obtained in Stockholm, Sweden, for routine diagnosis of influenza A virus, influenza B virus, respiratory syncytial (RS) virus, parainfluenza type 3 virus, and adenovirus infections by demonstration of viral antigens directly in the specimens. The cells in nasoph...

journal_title：Journal of clinical microbiology

authors： Grandien M,Pettersson CA,Gardner PS,Linde A,Stanton A

更新日期：1985-11-01 00:00:00

abstract：:Aberrant filamentous forms of Escherichia coli were observed on direct Gram stain of blood cultures from a patient being treated with the beta-lactam antibiotic cephalexin. After the institution of an alternative antibiotic regimen which included a different cell wall-active agent, E. coli of normal morphology was det...

journal_title：Journal of clinical microbiology

authors： Magnussen CR,Hruska JF

更新日期：1980-11-01 00:00:00