Reverse transcription-loop-mediated isothermal amplification assay for rapid detection of hepatitis E virus.

abstract：:The first World Health Organization (WHO) international standards (ISs) for nucleic acid amplification techniques were established two decades ago, with the initial focus on blood screening for three major viral targets, i.e., hepatitis C virus, hepatitis B virus, and human immunodeficiency virus 1. These reference ma...

journal_title：Journal of clinical microbiology

authors： Baylis SA,Wallace P,McCulloch E,Niesters HGM,Nübling CM

更新日期：2019-01-02 00:00:00

abstract：:Early and accurate diagnosis is essential for optimal therapeutic outcomes in patients infected with HIV. Currently, none of the commercially available fourth-generation assays differentiate HIV-1 and HIV-2 antibodies (Ab) or the HIV-1 p24 antigen (Ag). The aim of this study was to evaluate the performance of a novel ...

journal_title：Journal of clinical microbiology

authors： Salmona M,Delarue S,Delaugerre C,Simon F,Maylin S

更新日期：2014-01-01 00:00:00

abstract：:A polymerase chain reaction (PCR) procedure coupled to an enzyme-linked oligonucleotide sorbent assay (ELOSA; a PCR-ELOSA) identified all 24 serotypic variants of bluetongue virus (BTV) without identifying any of six viruses belonging to the related epizootic hemorrhagic disease virus serogroup. The PCR-ELOSA detected...

journal_title：Journal of clinical microbiology

authors： Katz JB,Alstad AD,Gustafson GA,Moser KM

更新日期：1993-11-01 00:00:00

abstract：:Evaluation of optimal compositions of blood culture media has called for extensive and laborious work in comparative studies of large series of clincal specimens. Bacterial growth is accompanied by heat production, and calorimetry provides an analytical tool for its detection and quantification. A twin microcalorimete...

journal_title：Journal of clinical microbiology

authors： Ripa KT,Mårdh PA,Hovelius B,Ljungholm K

更新日期：1977-04-01 00:00:00

abstract：:Three bovine group B rotavirus strains were detected from diarrheic calves during a surveillance study of rotaviral diarrhea in West Bengal, India. The sequence analysis of VP7 and NSP5 genes of these strains demonstrates a high degree of sequence variation from other group B rotavirus strains, indicating the emergenc...

journal_title：Journal of clinical microbiology

authors： Barman P,Ghosh S,Das S,Varghese V,Chaudhuri S,Sarkar S,Krishnan T,Bhattacharya SK,Chakrabarti A,Kobayashi N,Naik TN

更新日期：2004-06-01 00:00:00

abstract：:During a 1-year survey of Shiga toxin-producing Escherichia coli (STEC) prevalence in central France, 2,143 samples were investigated by PCR for Shiga toxin-encoding genes. A total of 330 (70%) of 471 fecal samples collected from healthy cattle at the Clermont-Ferrand slaughterhouse, 47 (11%) of 411 beef samples, 60 (...

journal_title：Journal of clinical microbiology

authors： Pradel N,Livrelli V,De Champs C,Palcoux JB,Reynaud A,Scheutz F,Sirot J,Joly B,Forestier C

更新日期：2000-03-01 00:00:00

abstract：:Aeromonas caviae was recovered as the sole potential enteric pathogen from the stools of 14 of 17 symptomatic children (10 younger than 1 year of age) while Aeromonas hydrophila, Aeromonas sobria, and Plesiomonas shigelloides were isolated once each. The infants from whom A. caviae was isolated all presented with a wa...

journal_title：Journal of clinical microbiology

authors： Namdari H,Bottone EJ

更新日期：1990-05-01 00:00:00

abstract：:The distribution of Chlamydia trachomatis serovars and Neisseria gonorrhoeae coinfection was studied in a group of 100 C. trachomatis-positive males with urethritis in Greece. The serovar distribution revealed that apart from the predominant worldwide types E and F, the relatively uncommon type G is also prevalent. Go...

journal_title：Journal of clinical microbiology

authors： Papadogeorgakis H,Pittaras TE,Papaparaskevas J,Pitiriga V,Katsambas A,Tsakris A

更新日期：2010-06-01 00:00:00

abstract：:Childhood tuberculosis (TB) presents significant diagnostic challenges associated with paucibacillary disease and requires a more sensitive test. We evaluated the diagnostic accuracy of Xpert MTB/RIF Ultra (Ultra) compared to other microbiological tests using respiratory samples from Ugandan children in the SHINE tria...

journal_title：Journal of clinical microbiology

authors： Ssengooba W,Iragena JD,Nakiyingi L,Mujumbi S,Wobudeya E,Mboizi R,Boulware D,Meya DB,Choo L,Crook AM,Lebeau K,Joloba M,Demers AM,Cresswell FV,Gibb DM

更新日期：2020-08-24 00:00:00

abstract：:Of 4,000 endocervical specimens tested with the Chlamydiazyme enzyme-linked immunosorbent assay (Abbott Laboratories), 233 (5.8%) gave positive results (A492 above the cutoff), which were confirmed with a blocking reagent (Abbott). An additional 34 specimens (14.6%) with chlamydial antigen were detected and confirmed ...

journal_title：Journal of clinical microbiology

authors： Kellogg JA,Seiple JW,Stroll ES

更新日期：1993-06-01 00:00:00

abstract：:A serum immunoglobulin G enzyme immunoassay (EIA) for Helicobacter pylori antibodies already in use in adults was evaluated with 99 pediatric serum samples to determine its usefulness for the study of H. pylori disease in children. The reference method used was either the (13)C-urea breath test or a biopsy culture of ...

journal_title：Journal of clinical microbiology

authors： Sunnerstam B,Kjerstadius T,Jansson L,Giesecke J,Bergström M,Ejderhamn J

更新日期：1999-10-01 00:00:00

abstract：:A clinical evaluation of the Amplicor polymerase chain reaction (PCR) assay for the detection of Chlamydia trachomatis in endocervical swabs (Roche Molecular Systems, Branchburg, N.J.) is described. This new clinical system used one-step sample preparation, amplification with biotinylated cryptic plasmid primer pairs ...

journal_title：Journal of clinical microbiology

authors： Bass CA,Jungkind DL,Silverman NS,Bondi JM

更新日期：1993-10-01 00:00:00

abstract：:We report a case of pneumonia with a fatal outcome caused by a nonhemolytic strain of Streptococcus pyogenes. This strain was isolated in pure growth from blood cultures and was fully identified biochemically. Such strains will be difficult to recognize and isolate from sites with heavy growth of normal flora. This ph...

journal_title：Journal of clinical microbiology

authors： Taylor MB,Barkham T

更新日期：2002-06-01 00:00:00

abstract：:We compared the results obtained with six commercial MIC test systems (Etest, MicroScan, Phoenix, Sensititre, Vitek Legacy, and Vitek 2 systems) and three reference methods (agar dilution, disk diffusion, and vancomycin [VA] agar screen [VScr]) with the results obtained by the Clinical and Laboratory Standards Institu...

journal_title：Journal of clinical microbiology

authors： Swenson JM,Anderson KF,Lonsway DR,Thompson A,McAllister SK,Limbago BM,Carey RB,Tenover FC,Patel JB

更新日期：2009-07-01 00:00:00

abstract：:A 2000-2001 U.S. Haemophilus influenzae surveillance study (n = 1,434) detected nine (0.6%) beta-lactamase-negative and ampicillin-resistant (BLNAR) isolates collected from two different hospitals. The MICs of ampicillin for all nine isolates were 4 microg/ml, with results being reproducible; and all nine isolates wer...

journal_title：Journal of clinical microbiology

authors： Karlowsky JA,Critchley IA,Blosser-Middleton RS,Karginova EA,Jones ME,Thornsberry C,Sahm DF

更新日期：2002-03-01 00:00:00

abstract：:Direct inoculation onto Granada medium (GM) in plates and tubes was compared to inoculation into a selective Todd-Hewitt broth (with 8 microg of gentamicin per ml and 15 microg of nalidixic acid per ml) for detection of group B streptococci (GBS) in pregnant women with 800 vaginal and 450 vaginoanorectal samples. Comp...

journal_title：Journal of clinical microbiology

authors： Rosa-Fraile M,Rodriguez-Granger J,Cueto-Lopez M,Sampedro A,Gaye EB,Haro JM,Andreu A

更新日期：1999-08-01 00:00:00

abstract：:A qualitative multiplex reverse transcription (RT)-PCR and liquid hybridization assay for the detection of human enteroviruses, rhinoviruses, parechoviruses, and Aichi virus was developed. Furthermore, a separate assay for the recognition of hepatitis A virus was established to complement the test pattern so that all ...

journal_title：Journal of clinical microbiology

authors： Jokela P,Joki-Korpela P,Maaronen M,Glumoff V,Hyypiä T

更新日期：2005-03-01 00:00:00

abstract：:Cryptococcus gattii emerged in 1999 in a distinct ecologic environment on Vancouver Island, Canada. Estimates of incubation period were derived from an analysis of travelers with discreet exposures to this region. Individual case incubation periods ranged from 2 to 11 months, with a median of 6 to 7 months. ...

journal_title：Journal of clinical microbiology

authors： MacDougall L,Fyfe M

更新日期：2006-05-01 00:00:00

abstract：:We report on the performance of a recently introduced commercial chessboard method using 12 antisera, in comparison with that of the 55-antiserum panel used in determining the serogroups and types (SGTs) of Streptococcus pneumoniae, both of which were carried out by a coagglutination technique. Of a total of 150 strai...

journal_title：Journal of clinical microbiology

authors： Lalitha MK,Thomas K,Kumar RS,Steinhoff MC

更新日期：1999-01-01 00:00:00

abstract：:Established in-house quantitative PCR (qPCR) assays to detect the Mycoplasma genitalium adhesion protein (MgPa) and the 16S rRNA gene were found to be comparable for screening purposes, with a kappa value of 0.97 (95% confidence interval [CI], 0.94 to 1.01) and no difference in bacterial load quantified (P = 0.4399). ...

journal_title：Journal of clinical microbiology

authors： Twin J,Taylor N,Garland SM,Hocking JS,Walker J,Bradshaw CS,Fairley CK,Tabrizi SN

更新日期：2011-03-01 00:00:00

abstract：:Candida dubliniensis is a newly recognized fungal pathogen causing mucosal disease in AIDS patients. Although preliminary studies indicate that most strains of C. dubliniensis are susceptible to established antifungal agents, fluconazole-resistant strains have been detected. Furthermore, fluconazole-resistant strains ...

journal_title：Journal of clinical microbiology

authors： Pfaller MA,Messer SA,Gee S,Joly S,Pujol C,Sullivan DJ,Coleman DC,Soll DR

更新日期：1999-03-01 00:00:00

abstract：:Recent studies have shown the public health importance of identifying individuals with acute human immunodeficiency virus infection (AHI); however, the cost of nucleic acid amplification testing (NAAT) makes individual testing of at-risk individuals prohibitively expensive in many settings. Pooled NAAT (or group testi...

journal_title：Journal of clinical microbiology

authors： Westreich DJ,Hudgens MG,Fiscus SA,Pilcher CD

更新日期：2008-05-01 00:00:00

abstract：:The noninvasive diagnosis of amebic liver abscess is challenging, as most patients at the time of diagnosis do not have a concurrent intestinal infection with Entamoeba histolytica. Fecal testing for E. histolytica parasite antigen or DNA is negative in most patients. A real-time PCR assay was evaluated for detection ...

journal_title：Journal of clinical microbiology

authors： Haque R,Kabir M,Noor Z,Rahman SM,Mondal D,Alam F,Rahman I,Al Mahmood A,Ahmed N,Petri WA Jr

更新日期：2010-08-01 00:00:00

abstract：:A broad range of viral and bacterial pathogens can cause acute respiratory tract infection. For rapid detection of a broad respiratory pathogen spectrum, multiplex real-time PCR is ideal. This study evaluated the performance of the new Luminex NxTAG Respiratory Pathogen Panel (NxTAG-RPP) in comparison with the BioFire...

journal_title：Journal of clinical microbiology

authors： Chen JHK,Lam HY,Yip CCY,Wong SCY,Chan JFW,Ma ESK,Cheng VCC,Tang BSF,Yuen KY

更新日期：2016-07-01 00:00:00

abstract：:INNO-LiPA and PapType human papillomavirus (HPV) genotyping assays were compared for detection of HPV genotypes on archival vulvar tissue. The INNO-LiPA assay detected 49 HPV-16 infections, compared with 47 detected by the PapType assay. The INNO-LiPA assay detected amplifiable DNA in 59 (91%) biopsy specimens, compar...

journal_title：Journal of clinical microbiology

authors： Tan SE,Garland SM,Rumbold AR,Tabrizi SN

更新日期：2011-11-01 00:00:00

abstract：:Noroviruses are the leading cause of outbreaks of gastroenteritis in the world. At present, norovirus genogroup II, genotype 4 (GII/4), strains are the most prevalent in many countries. In this study we investigated 55 outbreaks and 35 sporadic cases of norovirus-associated gastroenteritis in food handlers in food-cat...

journal_title：Journal of clinical microbiology

authors： Ozawa K,Oka T,Takeda N,Hansman GS

更新日期：2007-12-01 00:00:00

abstract：:During a 6-month study we critically evaluated the accuracy of the AutoMicrobic system Gram-Negative Identification Card (Vitek Systems, Inc., Hazelwood, Mo.) in identifying glucose-nonfermenting gram-negative bacilli by testing 419 selected isolates in parallel with a conventional reference method. Of 356 isolates in...

journal_title：Journal of clinical microbiology

authors： Plorde JJ,Gates JA,Carlson LG,Tenover FC

更新日期：1986-02-01 00:00:00

abstract：:Antibiotic neutralization in blood culture media from two automated systems was evaluated by measuring the recovery of organisms and times to detection in simulated cultures. Overall, BD Bactec Plus media (Bactec FX system) outperformed TREK 80 ml Redox media (VersaTREK system), although results suggest a relative rat...

journal_title：Journal of clinical microbiology

authors： Miller NS,Rogan D,Orr BL,Whitney D

更新日期：2011-04-01 00:00:00

abstract：:Blood culture bottles containing antibiotic binding resins are routinely used to minimize artificial sterilization in the presence of antibiotics. However, the resin binding kinetics can differ between antibiotics and concentrations. This study assessed the impact of clinically meaningful peak, midpoint, and trough co...

journal_title：Journal of clinical microbiology

authors： Chen IH,Nicolau DP,Kuti JL

更新日期：2019-09-24 00:00:00

abstract：:Inconsistent quality control results in disk diffusion testing of cefaclor, cefamandole, cefonicid, and cefuroxime with Haemophilus influenzae ATCC 49247 and Haemophilus test medium (HTM) prompted a search for an alternative control strain that would provide more reliable results. A five-laboratory study was conducted...

journal_title：Journal of clinical microbiology

authors： Jorgensen JH,Barry AL,Doern GV,Ferraro MJ,Murray PR

更新日期：1992-08-01 00:00:00