Fatal case of pneumonia caused by a nonhemolytic strain of Streptococcus pyogenes.

abstract：:A direct and an indirect enzyme immunoassay (EIA) were evaluated against a standard of electron microscopy after direct ultracentrifugation of the specimen for their performances in detecting rotaviruses. The indirect EIA had variable background activity which influenced test specificity. The indirect EIA control (tes...

journal_title：Journal of clinical microbiology

authors： Hammond GW,Ahluwalia GS,Barker FG,Horsman G,Hazelton PR

更新日期：1982-07-01 00:00:00

abstract：:The establishment of an optimal noninvasive method for diagnosing invasive aspergillosis (IA) is needed to improve the management of this life-threatening infection in patients with hematological disorders, and a number of noninvasive tests for IA that target different fungal components, including galactomannan, (1-->...

journal_title：Journal of clinical microbiology

authors： Kawazu M,Kanda Y,Nannya Y,Aoki K,Kurokawa M,Chiba S,Motokura T,Hirai H,Ogawa S

更新日期：2004-06-01 00:00:00

abstract：:We have evaluated the diagnostic utility of eleven Toxoplasma gondii recombinant antigens (P22 [SAG2], P24 [GRA1], P25, P28 [GRA2], P29 [GRA7], P30 [SAG1], P35, P41 [GRA4], P54 [ROP2], P66 [ROP1], and P68) in immunoglobulin G (IgG) and IgM recombinant enzyme-linked immunosorbent assays (Rec-ELISAs). Following an initi...

journal_title：Journal of clinical microbiology

authors： Aubert D,Maine GT,Villena I,Hunt JC,Howard L,Sheu M,Brojanac S,Chovan LE,Nowlan SF,Pinon JM

更新日期：2000-03-01 00:00:00

abstract：:We examined the reliability and accuracy of a colorimetric assay using Alamar Blue reagent in the performance of susceptibility tests for Candida albicans. We compared the broth macrodilution method recommended by the National Committee for Clinical Laboratory Standards (NCCLS) with a macrodilution method modified wit...

journal_title：Journal of clinical microbiology

authors： Tiballi RN,He X,Zarins LT,Revankar SG,Kauffman CA

更新日期：1995-04-01 00:00:00

abstract：:Vibrio cholerae O139 synonym Bengal recently caused large epidemics of cholera-like disease in Bangladesh and India. We compared the restriction fragment length polymorphisms of ctxA and rRNA genes (ribotypes) in 27 isolates of V. cholerae O139 from patients in Bangladesh and India with those of 48 isolates of V. chol...

journal_title：Journal of clinical microbiology

authors： Faruque SM,Abdul Alim AR,Roy SK,Khan F,Nair GB,Sack RB,Albert MJ

更新日期：1994-04-01 00:00:00

abstract：:Cowan I staphylococci coated with antisera to streptococcal groups A, B, C, D, F, and G were used as coagglutination reagents in a modified coagglutination procedure (MCAP). Streptococcal group antigens were extracted with a Streptomyces albus-lysozyme enzyme mixture for 30 min at 55 degrees C and centrifuged, and the...

journal_title：Journal of clinical microbiology

authors： Carlson JR,McCarthy LR

更新日期：1979-03-01 00:00:00

abstract：:A PCR specific for spacer regions 33 and 34 of the direct repeat region of the Mycobacterium tuberculosis complex was developed to complement the biochemical differentiation of M. tuberculosis, Mycobacterium bovis, M. bovis BCG, and Mycobacterium africanum subtypes I and II. In addition, this approach was incorporated...

journal_title：Journal of clinical microbiology

authors： Yeboah-Manu D,Yates MD,Wilson SM

更新日期：2001-11-01 00:00:00

abstract：:Rabies virus found worldwide and prevalent throughout the United States continues to be a public health concern. Direct-fluorescent antibody (DFA) detection remains the gold standard for rabies virus diagnostics. Assessing the utility of a high-throughput molecular platform such as the QIAsymphony SP/AS, in conjunctio...

journal_title：Journal of clinical microbiology

authors： Dupuis M,Brunt S,Appler K,Davis A,Rudd R

更新日期：2015-09-01 00:00:00

abstract：:A surveillance network was implemented by the Istituto Superiore di Sanità of Rome in collaboration with laboratories of virology in Czech Republic, Slovenia, Croatia, Albania, and Bulgaria. About 1,500 rotavirus-positive stool samples were collected from children with severe gastroenteritis admitted to hospitals or o...

journal_title：Journal of clinical microbiology

authors： Tcheremenskaia O,Marucci G,De Petris S,Ruggeri FM,Dovecar D,Sternak SL,Matyasova I,Dhimolea MK,Mladenova Z,Fiore L,Rotavirus Study Group.

更新日期：2007-07-01 00:00:00

abstract：:A sensitive rolling-circle amplification (RCA)-based method utilizing species-specific padlock probes targeted to the internal transcribed spacer 2 region of the fungal ribosomal DNA gene complex was developed. The assay was rapid (2 hours) and specific. Of 28 fungal isolates (16 of Candida, six of Aspergillus, and si...

journal_title：Journal of clinical microbiology

authors： Zhou X,Kong F,Sorrell TC,Wang H,Duan Y,Chen SC

更新日期：2008-07-01 00:00:00

abstract：:Selective media for Vibrio parahaemolyticus, BTB-Teepol agar and modified arabinose-ammonium sulfate-cholate agar, were modified for use in immunological detection of the thermostable direct hemolysin produced by this organism. The modified BTB-Teepol agar and modified arabinose-ammonium sulfate-cholate agar were both...

journal_title：Journal of clinical microbiology

authors： Honda T,Sornchai C,Takeda Y,Miwatani T

更新日期：1982-10-01 00:00:00

abstract：:Multidrug-resistant (MDR) Klebsiella pneumoniae is one of the most important causes of nosocomial infections worldwide. After the spread of strains resistant to beta-lactams at the end of the previous century, the diffusion of isolates resistant to carbapenems and colistin is now reducing treatment options and the con...

journal_title：Journal of clinical microbiology

authors： Onori R,Gaiarsa S,Comandatore F,Pongolini S,Brisse S,Colombo A,Cassani G,Marone P,Grossi P,Minoja G,Bandi C,Sassera D,Toniolo A

更新日期：2015-09-01 00:00:00

abstract：:Ten characteristic strains of spirochetes (HT2, HT7, HT10, HT15, HT17, HT19, HT20, HT22, HT32, and HT59) isolated from Ixodes persulcatus adult ticks in Hokkaido, Japan, were selected and analyzed by polyacrylamide gel electrophoresis, with monoclonal and polyclonal antibodies, and by pulsed-field gel electrophoresis....

journal_title：Journal of clinical microbiology

authors： Fukunaga M,Sohnaka M,Takahashi Y,Nakao M,Miyamoto K

更新日期：1993-05-01 00:00:00

abstract：:High-risk human papillomavirus (hrHPV) testing is now being introduced as a potential primary screening test for improved detection of cervical precancer and cancer. Current U.S. Food and Drug Administration-approved tests are batch tests that take several hours to complete. A rapid, non-batch test might permit point-...

journal_title：Journal of clinical microbiology

authors： Einstein MH,Smith KM,Davis TE,Schmeler KM,Ferris DG,Savage AH,Gray JE,Stoler MH,Wright TC Jr,Ferenczy A,Castle PE

更新日期：2014-06-01 00:00:00

abstract：:Microsporidia are protozoa parasites responsible for significant gastrointestinal disease in patients infected with human immunodeficiency virus. We evaluated a PCR assay of stool samples, duodenal aspirates, and biopsy specimens from patients with Enterocytozoon bieneusi infection. A 210-bp DNA fragment of the unique...

journal_title：Journal of clinical microbiology

authors： Velásquez JN,Carnevale S,Guarnera EA,Labbé JH,Chertcoff A,Cabrera MG,Rodríguez MI

更新日期：1996-12-01 00:00:00

abstract：:We studied nine solid and two liquid media for their suitability to select Aeromonas and Plesiomonas spp. from human stools, using artificially contaminated samples as well as 254 samples from outpatients with and without diarrhea. Media with optimal sensitivity and specificity for Aeromonas spp. were alkaline peptone...

journal_title：Journal of clinical microbiology

authors： von Graevenitz A,Bucher C

更新日期：1983-01-01 00:00:00

abstract：:Serial cerebrospinal fluid samples were obtained from 14 multiple sclerosis patients over a period of several months and were tested under code for Inoue-Melnick virus. In five of the positive patients, virus was present in 28 of 32 specimens collected over a period of 2 to 5 months. Four patients, from whom a total o...

journal_title：Journal of clinical microbiology

authors： Melnick JL,Seidel E,Wang SS,Muchinik G,Rashti EJ,Jacobs LD,Freeman AI,O'Malley J

更新日期：1985-10-01 00:00:00

abstract：:A new rapid one-step immunochromatographic test using monoclonal antibodies for detection of Helicobacter pylori antigen in stool in children was evaluated on coded stool samples from 159 children (mean age, 9.7 +/- 5.0 years; 118 from Munich, 41 from Vienna): 86 children were H. pylori infected defined by positive cu...

journal_title：Journal of clinical microbiology

authors： Antos D,Crone J,Konstantopoulos N,Koletzko S

更新日期：2005-06-01 00:00:00

abstract：:A panel (ENVA-1) of well-defined blinded samples containing wild-type and mutant human immunodeficiency virus type 1 (HIV-1) reverse transcriptase was analyzed by automated DNA sequencing in 23 laboratories worldwide. Drug resistance mutations at codons 41, 215, and 184 were present in the panel samples at different r...

journal_title：Journal of clinical microbiology

authors： Schuurman R,Demeter L,Reichelderfer P,Tijnagel J,de Groot T,Boucher C

更新日期：1999-07-01 00:00:00

abstract：:The IDI-MRSA assay (Infectio Diagnostic, Inc., Sainte-Foy, Quebec, Canada) with the Smart Cycler II rapid DNA amplification system (Cepheid, Sunnyvale, CA) appears to be sensitive and specific for the rapid detection of nasal colonization by methicillin-resistant Staphylococcus aureus (MRSA). We assessed the sensitivi...

journal_title：Journal of clinical microbiology

authors： Bishop EJ,Grabsch EA,Ballard SA,Mayall B,Xie S,Martin R,Grayson ML

更新日期：2006-08-01 00:00:00

abstract：:A 115-kDa exoantigen produced by Cryptococcus neoformans recognized by the previously described murine monoclonal antibody 7C9 has been purified from culture filtrate by a combination of membrane ultrafiltration, isoelectric focusing, and preparative gel electrophoresis. It is produced in late-log-phase cultures and i...

journal_title：Journal of clinical microbiology

authors： Hamilton AJ,Goodley J

更新日期：1993-02-01 00:00:00

abstract：:Direct enzyme immunoassay (Herpchek) was compared with culture on 21,522 specimens mainly from asymptomatic women for herpes simplex virus detection. Sensitivity and specificity were 73.8 and 97.7%, respectively. The 33% detection rate by enzyme immunoassay in 5 h increased to 43% when the enzyme immunoassay was combi...

journal_title：Journal of clinical microbiology

authors： Verano L,Michalski FJ

更新日期：1995-05-01 00:00:00

abstract：:We report a case of staphylococcal exanthematous disease in a newborn due to a toxic shock syndrome toxin 1- and SEC-producing methicillin-resistant Staphylococcus aureus strain and alert neonatologists to the probable emergence in France of the neonatal toxic shock syndrome-like exanthematous disease in newborns prev...

journal_title：Journal of clinical microbiology

authors： van der Mee-Marquet N,Lina G,Quentin R,Yaouanc-Lapalle H,Fièvre C,Takahashi N,Etienne J

更新日期：2003-10-01 00:00:00

abstract：:During a survey of Group G and C streptococcal infections of humans two epidemiologically unrelated Group G streptococcal isolates were identified, one from a case of bacteremia and one from a wound infection. These isolates were atypical among this sample in that the emm gene could not be amplified from them by PCR. ...

journal_title：Journal of clinical microbiology

authors： Whatmore AM,Engler KH,Gudmundsdottir G,Efstratiou A

更新日期：2001-11-01 00:00:00

abstract：:Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and conventional standard methods were compared for time to pathogen identification and impact on clinical outcomes in peritoneal dialysis-related peritonitis patients. The MALDI-TOF MS method identified the causative microorga...

journal_title：Journal of clinical microbiology

authors： Lin WH,Hwang JC,Tseng CC,Chang YT,Wu AB,Yan JJ,Wu JJ,Wang MC

更新日期：2016-05-01 00:00:00

abstract：:The biochemical characteristics and the isolation rates of the two Lancefield group C streptococcal species (S. equisimilis and S. anginosus) from patients with pharyngitis and asymptomatic controls were compared. Some 239 strains of beta-hemolytic group C streptococci were isolated from 1,480 patients (209 strains) w...

journal_title：Journal of clinical microbiology

authors： Fox K,Turner J,Fox A

更新日期：1993-04-01 00:00:00

abstract：:The one-step single-tube betaine-free reverse transcription (RT)-loop-mediated isothermal amplification assay was developed for rapid diagnosis of hepatitis E virus. This assay amplified the target gene in less than 45 min (even as short as 20 min) under isothermal conditions at 63 degrees C, and the sensitivity of th...

journal_title：Journal of clinical microbiology

authors： Lan X,Yang B,Li BY,Yin XP,Li XR,Liu JX

更新日期：2009-07-01 00:00:00

abstract：:Invasive pulmonary aspergillosis (IPA) is a frequently fatal infection in immunocompromised patients that is difficult to diagnose. Present methods for detection of Aspergillus spp. in bronchoalveolar lavage (BAL) fluid and in tissue vary in sensitivity and specificity. We therefore developed an A. fumigatus-specific ...

journal_title：Journal of clinical microbiology

authors： O'Sullivan CE,Kasai M,Francesconi A,Petraitis V,Petraitiene R,Kelaher AM,Sarafandi AA,Walsh TJ

更新日期：2003-12-01 00:00:00

abstract：:A simple procedure for the selective isolation of the protective species-specific protein antigens (SPAs) of Rickettsia typhi and Rickettsia prowazekii was developed to permit use of the SPAs in the immunodiagnosis and immunoprophylaxis of typhus infections. Although the SPAs were readily extracted from lysozyme- or d...

journal_title：Journal of clinical microbiology

authors： Dasch GA

更新日期：1981-09-01 00:00:00

abstract：:Diagnosis of Bordetella pertussis infection has been difficult due to the low sensitivity of culture. PCR tests have been shown to be more sensitive than culture, but the reported sensitivity of PCR is variable. We evaluated PCR product detection by using either agarose gel electrophoresis (PCR-gel) or dot blot hybrid...

journal_title：Journal of clinical microbiology

authors： Lingappa JR,Lawrence W,West-Keefe S,Gautom R,Cookson BT

更新日期：2002-08-01 00:00:00