Comparison of Automated Quantitative Reverse Transcription-PCR and Direct Fluorescent-Antibody Detection for Routine Rabies Diagnosis in the United States.

abstract：:A new direct immunofluorescence monoclonal antibody (DFA) method (Genetic Systems, Inc., Seattle, Wash.) for identification of Pneumocystis carinii in induced sputum and bronchoalveolar lavage specimens was compared in a blinded study with an established Giemsa stain method. We evaluated 148 consecutive clinical speci...

journal_title：Journal of clinical microbiology

authors： Wolfson JS,Waldron MA,Sierra LS

更新日期：1990-09-01 00:00:00

abstract：:Intravenous and intraarterial catheters were examined microbiologically and morphologically. Bacteria or yeasts were recovered from 38 of the 63 catheters examined, and Staphylococcus epidermidis was present on 29 of the 38 colonized catheters. Examination of unused Teflon catheters ( Jelco ; Surgikos , Inc., Peterbor...

journal_title：Journal of clinical microbiology

authors： Marrie TJ,Costerton JW

更新日期：1984-05-01 00:00:00

abstract：:Paecilomyces variotii is a commonly occurring species in air and food, but it is also associated with many types of human infections and is among the emerging causative agents of opportunistic mycoses in immunocompromised hosts. Paecilomyces can cause hyalohyphomycosis, and two species, Paecilomyces lilacinus and P. v...

journal_title：Journal of clinical microbiology

authors： Houbraken J,Verweij PE,Rijs AJ,Borman AM,Samson RA

更新日期：2010-08-01 00:00:00

abstract：:We present a duplex, real-time PCR assay for detection of Klebsiella pneumoniae carbapenemase (blaKPC) and New Delhi metallo-β-lactamase (blaNDM) genes. Accuracy was assessed with 158 Gram-negative bacillary isolates, including 134 carbapenemase producers. The assay had 100% sensitivity and specificity compared with r...

journal_title：Journal of clinical microbiology

authors： Cunningham SA,Noorie T,Meunier D,Woodford N,Patel R

更新日期：2013-04-01 00:00:00

abstract：:This is the first report in Israel of a cluster of cases of diarrhea associated with the isolation of Escherichia coli O157 in stool samples. E. coli O157:NM (nonmotile), producing verotoxins 1 and 2 was isolated from the stool samples of three infants less than 1 year of age with diarrhea and from a fourth asymptomat...

journal_title：Journal of clinical microbiology

authors： Lerman Y,Cohen D,Gluck A,Ohad E,Sechter I

更新日期：1992-02-01 00:00:00

abstract：:We used simulated blood cultures inoculated with clinical isolates of Mycoplasma hominis to determine whether liquid media of the BacT/ALERT (Organon Teknika, Durham, N.C.) will support growth of this fastidious organism and whether its presence can generate a positive signal with the instrument. Viability of clinical...

journal_title：Journal of clinical microbiology

authors： Waites KB,Canupp KC

更新日期：2001-12-01 00:00:00

abstract：:For recovery of brucellae from clinical specimens, a lysis-concentration procedure was superior to the procedure of Castañeda (M. R. Castañeda, Proc. Soc. Exp. Biol. Med. 64:114-115, 1947). ...

journal_title：Journal of clinical microbiology

authors： Etemadi H,Raissadat A,Pickett MJ,Zafari Y,Vahedifar P

更新日期：1984-09-01 00:00:00

abstract：:We report the isolation of Pseudomonas putrefaciens from an intra-abdominal abscess in a patient with colonic carcinoma and from bile in two patients with biliary tract disease. In all three cases, P. putrefaciens was isolated in mixed culture with enteric bacteria. ...

journal_title：Journal of clinical microbiology

authors： Marne C,Pallarés R,Sitges-Serra A

更新日期：1983-06-01 00:00:00

abstract：:tRNA intergenic spacer PCR (tDNA-PCR) was evaluated for its usefulness in the differentiation of enterococcal species of human and animal origin. This technique was carried out for 124 strains belonging to 17 enterococcal species and generated DNA fragments, which were separated by capillary electrophoresis. tDNA-PCR ...

journal_title：Journal of clinical microbiology

authors： Baele M,Baele P,Vaneechoutte M,Storms V,Butaye P,Devriese LA,Verschraegen G,Gillis M,Haesebrouck F

更新日期：2000-11-01 00:00:00

abstract：:Rotavirus RNA was detected in the cerebrospinal fluid (CSF) of a child with central nervous system disease symptoms associated with rotavirus gastroenteritis. The rotavirus isolates from the fecal and CSF samples were genotyped as G1P[8]. Sequence analysis of the VP7 and VP4 proteins derived from the fecal and CSF sam...

journal_title：Journal of clinical microbiology

authors： Iturriza-Gómara M,Auchterlonie IA,Zaw W,Molyneaux P,Desselberger U,Gray J

更新日期：2002-12-01 00:00:00

abstract：:Resistance to antimonial drugs has been documented in Leishmania isolates transmitted in South America, Europe, and Asia. The frequency and distribution of resistance to these and other antileishmanial drugs are unknown. Technical constraints have limited the assessment of drug susceptibility of clinical strains of Le...

journal_title：Journal of clinical microbiology

authors： Fernández O,Diaz-Toro Y,Valderrama L,Ovalle C,Valderrama M,Castillo H,Perez M,Saravia NG

更新日期：2012-07-01 00:00:00

abstract：:We compared the results obtained with six commercial MIC test systems (Etest, MicroScan, Phoenix, Sensititre, Vitek Legacy, and Vitek 2 systems) and three reference methods (agar dilution, disk diffusion, and vancomycin [VA] agar screen [VScr]) with the results obtained by the Clinical and Laboratory Standards Institu...

journal_title：Journal of clinical microbiology

authors： Swenson JM,Anderson KF,Lonsway DR,Thompson A,McAllister SK,Limbago BM,Carey RB,Tenover FC,Patel JB

更新日期：2009-07-01 00:00:00

abstract：:To establish the exact pathogenic role of Mycobacterium abscessus in cystic fibrosis (CF), molecular tests are required for accurate identification. Forty-eight M. abscessus isolates from seven patients with CF were analyzed by sequence analysis for sequence variants within the hsp65 gene and the 16S-23S intergenic se...

journal_title：Journal of clinical microbiology

authors： König B,Tammer I,Sollich V,König W

更新日期：2005-07-01 00:00:00

abstract：:In this study, 5-iodo-2'-deoxyuridine-, cytochalasin B-, and cycloheximide-treated and untreated McCoy cells were inoculated with representative strains of Chlamydia trachomatis serotypes, as well as with clinical specimens from a pediatric population, and the quality and quantity of the cytoplasmic inclusions were ex...

journal_title：Journal of clinical microbiology

authors： La Scolea LJ Jr,Keddell JE

更新日期：1981-04-01 00:00:00

abstract：:A new molecular biological approach for the identification of bacteria is described. This approach employs PCR of bacterial cell lysates with conserved primers located in the 16S rRNA sequence flanking a variable region, and analysis of the amplified product was based on the principle of single-strand conformation pol...

journal_title：Journal of clinical microbiology

authors： Widjojoatmodjo MN,Fluit AC,Verhoef J

更新日期：1994-12-01 00:00:00

abstract：:Of 100 strains of enterococci, 83% reduced litmus milk within 4 h. None of the 100 non-enterococcal streptococci tested were positive. ...

journal_title：Journal of clinical microbiology

authors： Schierl EA,Blazevic DJ

更新日期：1981-08-01 00:00:00

abstract：:Campylobacter jejuni (20 strains) and Campylobacter coli (12 strains) were assigned to four biovars for each species based on phenotypic tests that were easy to perform and interpret. The resulting biotyping schemes offer a greater degree of distinction among C. jejuni and C. coli strains than any of the other biotypi...

journal_title：Journal of clinical microbiology

authors： Roop RM 2nd,Smibert RM,Krieg NR

更新日期：1984-11-01 00:00:00

abstract：:An association between (unculturable) gastrospirillum-like organisms (GLO) and ulcerative lesions in the pars oesophagea in stomachs of swine has been claimed. In dogs GLO detected by microscopy may represent several Helicobacter species or subspecies. Therefore we investigated which Helicobacter spp. are present in s...

journal_title：Journal of clinical microbiology

authors： Roosendaal R,Vos JH,Roumen T,van Vugt R,Cattoli G,Bart A,Klaasen HL,Kuipers EJ,Vandenbroucke-Grauls CM,Kusters JG

更新日期：2000-07-01 00:00:00

abstract：:Enterococcus hirae is a rare isolate in clinical specimens. We describe a case of native aortic-valve endocarditis that was caused by Enterococcus hirae in a 72-year-old man. This is the first reported case of endocarditis due to this organism. ...

journal_title：Journal of clinical microbiology

authors： Poyart C,Lambert T,Morand P,Abassade P,Quesne G,Baudouy Y,Trieu-Cuot P

更新日期：2002-07-01 00:00:00

abstract：:The newly emerged Middle East respiratory syndrome coronavirus (MERS-CoV) has infected at least 1,082 people, including 439 fatalities. So far, no empirical virus isolation study has been done to elucidate infectious virus secretion or serotype variability. Here, we used 51 respiratory samples from 32 patients with co...

journal_title：Journal of clinical microbiology

authors： Muth D,Corman VM,Meyer B,Assiri A,Al-Masri M,Farah M,Steinhagen K,Lattwein E,Al-Tawfiq JA,Albarrak A,Müller MA,Drosten C,Memish ZA

更新日期：2015-09-01 00:00:00

abstract：:Vaginal swab specimens may be preferable to cervical swab or urine specimens for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae because of the ease of specimen collection and transport. The purpose of this study was to evaluate whether vaginal swab specimens are equivalent to cervical swab specimens ...

journal_title：Journal of clinical microbiology

authors： Cosentino LA,Landers DV,Hillier SL

更新日期：2003-08-01 00:00:00

abstract：:First-catch early morning urine samples from patients attending a genitourinary medicine clinic were tested by three different enzyme immunoassays. The results suggest significant differences in the sensitivities and specificities of the different assays. The direct visualization of elementary bodies in urine deposits...

journal_title：Journal of clinical microbiology

authors： Paul ID,Caul EO

更新日期：1990-02-01 00:00:00

abstract：:Two cases of primary invasive cutaneous infections caused by the zoophilic dermatophytic species Microsporum canis are presented. The first case occurred in a liver transplant recipient who was receiving immunosuppressive therapy. Multiple erythematous papules were seen on both legs, and a biopsy revealed invasive fun...

journal_title：Journal of clinical microbiology

authors： King D,Cheever LW,Hood A,Horn TD,Rinaldi MG,Merz WG

更新日期：1996-02-01 00:00:00

abstract：:Sixteen metabolically deficient dwarf-colony mutants of Escherichia coli were isolated from urine culture and represented about 2% of all E. coli isolated during the same period. In 14 cases, mutants were isolated from debilitated patients: elderly persons or patients in the terminal stages of a chronic disease. In 15...

journal_title：Journal of clinical microbiology

authors： Borderon E,Horodniceanu T

更新日期：1978-12-01 00:00:00

abstract：:Outbreaks of food-borne listeriosis have often involved strains of serotype 4b. Examination of multiple isolates from three different outbreaks revealed that ca. 11 to 29% of each epidemic population consisted of strains which were negative with the serotype-specific monoclonal antibody c74.22, lacked galactose from t...

journal_title：Journal of clinical microbiology

authors： Clark EE,Wesley I,Fiedler F,Promadej N,Kathariou S

更新日期：2000-10-01 00:00:00

abstract：:The performance of CHROMagar STEC and CHROMagar STEC O104 (CHROMagar Microbiology, Paris, France) media for the detection of Shiga toxin-producing Escherichia coli (STEC) was assessed with 329 stool specimens collected over 14 months from patients with suspected STEC infections (June 2011 to August 2012). The CHROMaga...

journal_title：Journal of clinical microbiology

authors： Gouali M,Ruckly C,Carle I,Lejay-Collin M,Weill FX

更新日期：2013-03-01 00:00:00

abstract：:We compared examination of unstained and Gram-stained smears as methods of evaluating sputum quality. Of 100 slides examined, 96 (96%) were graded identically. Each discrepancy between methods was near the cutoff for specimen acceptability. We concluded that examination of unstained smears can provide an accurate, cos...

journal_title：Journal of clinical microbiology

authors： Wetterau LM,Zeimis RT,Hollick GE

更新日期：1986-07-01 00:00:00

abstract：:We studied the prevalence and species distribution of nontuberculous mycobacteria (NTM) in relation to age in 385 patients with cystic fibrosis (CF) (mean age +/- standard deviation [range], 12.0 +/- 6.1 [1 to 24] years; sex ratio, 0.53) attending three Parisian centers. The overall prevalence of NTM in sputum was 8.1...

journal_title：Journal of clinical microbiology

authors： Pierre-Audigier C,Ferroni A,Sermet-Gaudelus I,Le Bourgeois M,Offredo C,Vu-Thien H,Fauroux B,Mariani P,Munck A,Bingen E,Guillemot D,Quesne G,Vincent V,Berche P,Gaillard JL

更新日期：2005-07-01 00:00:00

abstract：:Twenty-three nasal swab samples that tested positive for methicillin-resistant Staphylococcus aureus (MRSA) on initial testing by the BD GeneOhm MRSA assay (BD-MRSA PCR; BD GeneOhm, San Diego, CA) were culture positive only for methicillin-susceptible S. aureus (MSSA) from an enrichment broth. The 23 recovered isolate...

journal_title：Journal of clinical microbiology

authors： Stamper PD,Louie L,Wong H,Simor AE,Farley JE,Carroll KC

更新日期：2011-04-01 00:00:00

abstract：:We compared the use of bacteriophage sensitivity, seroagglutination with polyclonal antisera raised in rabbits or horses, seroagglutination with murine monoclonal antibody, and the serum agar precipitin technique for the detection of K1 capsular polysaccharide among clinical isolates of Escherichia coli obtained from ...

journal_title：Journal of clinical microbiology

authors： Cross A,Orskov I,Orskov F,Sadoff J,Gemski P

更新日期：1984-08-01 00:00:00