Rapid identification of bacteria by PCR-single-strand conformation polymorphism.

abstract：:Porphyromonas gingivalis is one of the major causative agents of adult periodontitis. One of the features of this periodontal pathogen is its ability to attach to a variety of oral bacterial surfaces and to colonize subgingival dental plaque. We have shown that Streptococcus cristatus CC5A inhibits expression of fimA,...

journal_title：Journal of clinical microbiology

authors： Wang BY,Wu J,Lamont RJ,Lin X,Xie H

更新日期：2009-12-01 00:00:00

abstract：:Botulism developed in a patient following surgical repair of an open radial fracture. Symptoms resolved after treatment with antitoxin and antibiotics, and hardware excision was deferred. Subsequent osteomyelitis necessitated hardware exchange, and wound cultures grew Clostridium argentinense. This case highlights the...

journal_title：Journal of clinical microbiology

authors： Taylor SM,Wolfe CR,Dixon TC,Ruch DS,Cox GM

更新日期：2010-02-01 00:00:00

abstract：:Actinomyces naeslundii is a saprophyte, sometimes a pathogen, of the human oral cavity. Very few extra-oral infections related to this agent have been described. We report the first instance of A. naeslundii as an etiological agent of pelvic actinomycosis in a user of an intrauterine device, an infection so far exclus...

journal_title：Journal of clinical microbiology

authors： Bonnez W,Lattimer G,Mohanraj NA,Johnson TH

更新日期：1985-02-01 00:00:00

abstract：:Formalin-fixed, purified reticulate bodies (RB) of Chlamydia trachomatis immunotype C/TW-3/OT were used as a serological test antigen in the microimmunofluorescence test. The sensitivity and specificity of the RB antigen were compared to elementary bodies (EB) used as antigens in the detection of C. trachomatis antibo...

journal_title：Journal of clinical microbiology

authors： Yong EC,Chinn JS,Caldwell HD,Kuo CC

更新日期：1979-09-01 00:00:00

abstract：:A simple procedure for the selective isolation of the protective species-specific protein antigens (SPAs) of Rickettsia typhi and Rickettsia prowazekii was developed to permit use of the SPAs in the immunodiagnosis and immunoprophylaxis of typhus infections. Although the SPAs were readily extracted from lysozyme- or d...

journal_title：Journal of clinical microbiology

authors： Dasch GA

更新日期：1981-09-01 00:00:00

abstract：:Nocardia takedensis is a recently described species isolated from soil. The first clinical isolate in Japan has recently been reported. This report describes the first clinical isolate of N. takedensis in Spain from a respiratory specimen. ...

journal_title：Journal of clinical microbiology

authors： Betrán A,Rezusta A,Lezcano MA,Villuendas MC,Revillo MJ,Boiron P,Rodríguez-Nava V

更新日期：2009-06-01 00:00:00

abstract：:In Europe, hemorrhagic fever with renal syndrome results mainly from infection with Puumala virus (PUUV) or Dobrava virus. For 31 patients from a hantavirus disease outbreak in Lower Bavaria, a district in southeast Germany, serodiagnosis was undertaken by enzyme-linked immunosorbent assay, immunofluorescence assay, a...

journal_title：Journal of clinical microbiology

authors： Schilling S,Emmerich P,Klempa B,Auste B,Schnaith E,Schmitz H,Krüger DH,Günther S,Meisel H

更新日期：2007-09-01 00:00:00

abstract：:New Delhi metallo-β-lactamase (NDM)-producing bacteria are considered potential global health threats. It is necessary to monitor NDM-1 and its variants in clinical isolates in order to understand the NDM-1 epidemic and the impact of its variants on β-lactam resistance. To reduce the lengthy time needed for cloning an...

journal_title：Journal of clinical microbiology

authors： Huang L,Hu X,Zhou M,Yang Y,Qiao J,Wang D,Yu J,Cui Z,Zhang Z,Zhang XE,Wei H

更新日期：2014-06-01 00:00:00

abstract：:Blood culture records from 1994 to 1995 from five U.S. medical centers all using the Difco ESP continuous monitoring blood culture system were reviewed retrospectively. Among a total of 7,362 isolates of bacteria and yeasts, only 0.1% of possibly significant isolates would have been missed had blood cultures been rout...

journal_title：Journal of clinical microbiology

authors： Doern GV,Brueggemann AB,Dunne WM,Jenkins SG,Halstead DC,McLaughlin JC

更新日期：1997-05-01 00:00:00

abstract：:The fungus Fusarium sacchari was isolated repeatedly from the blood of an immunosuppressed host. The infection was treated successfully with a small dose of amphotericin B. The strain was resistant to this antifungal in vitro. MICs and minimum fungicidal concentrations of six antifungals for the clinical isolate are p...

journal_title：Journal of clinical microbiology

authors： Guarro J,Nucci M,Akiti T,Gené J,Barreiro MD,Gonçalves RT

更新日期：2000-01-01 00:00:00

abstract：:CHROMagar Candida is a selective and differential chromogenic medium that has been shown to be useful for identification of Candida albicans, Candida krusei, Candida tropicalis, and perhaps Candida glabrata. Colony morphology and color have been well defined when CHROMagar Candida has been used to isolate yeast direct...

journal_title：Journal of clinical microbiology

authors： Horvath LL,Hospenthal DR,Murray CK,Dooley DP

更新日期：2003-06-01 00:00:00

abstract：:A proficiency review of antituberculous drug susceptibility testing (DST) was undertaken by the regional tuberculosis reference laboratories of the Western Pacific Region of WHO to evaluate the performance of national reference laboratories (NRLs) and to ensure that the results from the participating laboratories are ...

journal_title：Journal of clinical microbiology

authors： Bai GH,Kim SJ,Chang CL,National and Regional Tuberculosis Reference Laboratories.

更新日期：2007-11-01 00:00:00

abstract：:Serial cerebrospinal fluid samples were obtained from 14 multiple sclerosis patients over a period of several months and were tested under code for Inoue-Melnick virus. In five of the positive patients, virus was present in 28 of 32 specimens collected over a period of 2 to 5 months. Four patients, from whom a total o...

journal_title：Journal of clinical microbiology

authors： Melnick JL,Seidel E,Wang SS,Muchinik G,Rashti EJ,Jacobs LD,Freeman AI,O'Malley J

更新日期：1985-10-01 00:00:00

abstract：:During measles outbreaks, it is important to be able to rapidly distinguish between measles cases and vaccine reactions to avoid unnecessary outbreak response measures such as case isolation and contact investigations. We have developed a real-time reverse transcription-PCR (RT-PCR) method specific for genotype A meas...

journal_title：Journal of clinical microbiology

authors： Roy F,Mendoza L,Hiebert J,McNall RJ,Bankamp B,Connolly S,Lüdde A,Friedrich N,Mankertz A,Rota PA,Severini A

更新日期：2017-03-01 00:00:00

abstract：:The emergence of azole-resistant strains of Aspergillus fumigatus during treatment for aspergillosis occurs by a mutation selection process. Understanding how antifungal resistance mechanisms evolve in the host environment during infection is of great clinical importance and biological interest. Here, we used next-gen...

journal_title：Journal of clinical microbiology

authors： Hagiwara D,Takahashi H,Watanabe A,Takahashi-Nakaguchi A,Kawamoto S,Kamei K,Gonoi T

更新日期：2014-12-01 00:00:00

abstract：:Alere i RSV is a novel rapid test which applies a nicking enzyme amplification reaction to detect respiratory syncytial virus in point-of-care settings. In this study, we evaluated the Alere i RSV assay by using frozen nasopharyngeal swab samples that were collected in viral transport medium from children hospitalized...

journal_title：Journal of clinical microbiology

authors： Peters RM,Schnee SV,Tabatabai J,Schnitzler P,Pfeil J

更新日期：2017-04-01 00:00:00

abstract：:A technique is described which may be used to identify Haemophilus influenzae type b, Streptococcus pneumoniae, group A and B streptococci, and Staphylococcus aureus in smears of clinical specimens. ...

journal_title：Journal of clinical microbiology

authors： Clausen CR

更新日期：1981-06-01 00:00:00

abstract：:Vibrio metschnikovii and Vibrio gazogenes are two new Vibrio species that have been little studied. Thirteen strains of V. metschnikovii were highly related to the type strain, NCTC 8443, by DNA-DNA hybridization. Relatedness values were 83 to 90% at 60 degrees C and 75 to 84% at the more stringent 75 degrees C. Diver...

journal_title：Journal of clinical microbiology

authors： Farmer JJ 3rd,Hickman-Brenner FW,Fanning GR,Gordon CM,Brenner DJ

更新日期：1988-10-01 00:00:00

abstract：:Two multiplex PCRs were developed for the detection of necrotoxigenic Escherichia coli virulence genes. M1 contained the primers for the toxins and the aerobactin, and M2 contained the primers for the adhesins. They were validated by single PCRs performed with reference E. coli strains and by multiplex PCRs with necro...

journal_title：Journal of clinical microbiology

authors： Van Bost S,Jacquemin E,Oswald E,Mainil J

更新日期：2003-09-01 00:00:00

abstract：:Herpes simplex virus (HSV) causes acute and relapsing symptoms characterized by ulcerative lesions. Laboratory diagnosis of HSV in cutaneous or mucocutaneous lesions has historically been performed with the use of viral cell culture systems; however, these tests are laborious and suffer decreased sensitivity for advan...

journal_title：Journal of clinical microbiology

authors： Faron ML,Ledeboer NA,Patel A,Beqa SH,Yen-Lieberman B,Kohn D,Leber AL,Mayne D,Northern WI,Buchan BW

更新日期：2016-08-01 00:00:00

abstract：:Many diseases with unknown etiology may be caused by unidentified viruses. Sequence-independent amplification revealed a new astrovirus, similar to VA1, in a 4-year-old male diagnosed with celiac disease. This expands the geographic range of this virus to include Europe and may associate astrovirus infection with the ...

journal_title：Journal of clinical microbiology

authors： Smits SL,van Leeuwen M,van der Eijk AA,Fraaij PL,Escher JC,Simon JH,Osterhaus AD

更新日期：2010-09-01 00:00:00

abstract：:Family members of hepatitis C virus (HCV)-infected hemodialysis patients had a high rate (8.1%) of HCV infection and were often infected by the same genotype of HCV as the renal patients. Renal patients who had a longer history of hemodialysis or more frequent arteriovenous shunt replacement posed a higher risk to the...

journal_title：Journal of clinical microbiology

authors： Ho MS,Yang CS,Chen PJ,Mau YC

更新日期：1994-11-01 00:00:00

abstract：:We report on the development of a fully automated real-time PCR assay for the quantitative detection of hepatitis B virus (HBV) DNA in plasma with EDTA (EDTA plasma). The MagNA Pure LC instrument was used for automated DNA purification and automated preparation of PCR mixtures. Real-time PCR was performed on the Light...

journal_title：Journal of clinical microbiology

authors： Leb V,Stöcher M,Valentine-Thon E,Hölzl G,Kessler H,Stekel H,Berg J

更新日期：2004-02-01 00:00:00

abstract：:The recent emergence of the multidrug-resistant and pathogenic yeast Candida auris continues to cause public health concern worldwide. C. auris is alarming because it causes health care-associated outbreaks and can establish invasive infections with high mortality rates. Transmission between patients is facilitated by...

journal_title：Journal of clinical microbiology

authors： Sexton DJ,Kordalewska M,Bentz ML,Welsh RM,Perlin DS,Litvintseva AP

更新日期：2018-11-27 00:00:00

abstract：:Serum samples collected from 68 patients (age, mean +/- the standard deviation [SD], 56.3 +/- 12.8 years) at admission who were subsequently molecularly diagnosed as having hepatitis E and from 2,781 individuals who were assumed not to have been recently infected with hepatitis E virus (HEV; negative controls; 52.9 +/...

journal_title：Journal of clinical microbiology

authors： Takahashi M,Kusakai S,Mizuo H,Suzuki K,Fujimura K,Masuko K,Sugai Y,Aikawa T,Nishizawa T,Okamoto H

更新日期：2005-01-01 00:00:00

abstract：:We used simulated blood cultures inoculated with clinical isolates of Mycoplasma hominis to determine whether liquid media of the BacT/ALERT (Organon Teknika, Durham, N.C.) will support growth of this fastidious organism and whether its presence can generate a positive signal with the instrument. Viability of clinical...

journal_title：Journal of clinical microbiology

authors： Waites KB,Canupp KC

更新日期：2001-12-01 00:00:00

abstract：:After repeated use of fluconazole for therapy of oropharyngeal candidosis, the emergence of in vitro fluconazole-resistant Candida albicans isolates (MIC, > or = 25 micrograms/ml) together with oral candidosis unresponsive to oral dosages of up to 400 mg of fluconazole were observed in patients with human immunodefici...

journal_title：Journal of clinical microbiology

authors： Ruhnke M,Eigler A,Tennagen I,Geiseler B,Engelmann E,Trautmann M

更新日期：1994-09-01 00:00:00

abstract：:A total of 244 strains of Aerococcus, Enterococcus, and Streptococcus species were tested by the RapID STR system (Innovative Diagnostic Systems Inc., Atlanta, Ga.) for identification. Strains were identified without additional tests or with additional conventional tests suggested by the IDS compendium manual. Our dat...

journal_title：Journal of clinical microbiology

authors： You MS,Facklam RR

更新日期：1986-10-01 00:00:00

abstract：:Todd-Hewitt broth (THB) containing colistin and nalidixic acid was compared with four other media with respect to efficacy of isolation of group B streptocci. It was effective as plain THB, THB with gentamicin and nalidixic acid and blood, and THB with colistin, nalidixic acid, and blood. THB with gentamicin and nalid...

journal_title：Journal of clinical microbiology

authors： Fenton LJ,Harper MH

更新日期：1979-02-01 00:00:00

abstract：:In this study, 102 herpes simplex virus isolates were typed by cell culture selection (chicken embryo cells and guinea pig embryo cells [CE/GPE]), (E)-5-(2-bromovinyl)-2'-deoxyuridine (BVDU) sensitivity, plaque reduction neutralization, and indirect immunofluorescence staining techniques. The percentages of agreement ...

journal_title：Journal of clinical microbiology

authors： Zheng ZM,Mayo DR,Hsiung GD

更新日期：1983-03-01 00:00:00