Abstract:
:In Europe, hemorrhagic fever with renal syndrome results mainly from infection with Puumala virus (PUUV) or Dobrava virus. For 31 patients from a hantavirus disease outbreak in Lower Bavaria, a district in southeast Germany, serodiagnosis was undertaken by enzyme-linked immunosorbent assay, immunofluorescence assay, and immunoblot analysis. In a few of these cases, however, PUUV-specific typing of antibodies by these standard assays failed and a virus neutralization assay under biosafety level 3 conditions was required to verify the infection by this virus type. PUUV RNA was amplified by reverse transcription-PCR from acute-phase sera of three patients and was found to be very closely related to virus sequences obtained from bank voles (Clethrionomys glareolus) trapped in the same area. These findings link the outbreak with a novel PUUV lineage, "Bavaria," circulating in the local rodent population. The Bavaria lineage associated with the outbreak is only distantly related to other PUUV lineages from Germany.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Schilling S,Emmerich P,Klempa B,Auste B,Schnaith E,Schmitz H,Krüger DH,Günther S,Meisel Hdoi
10.1128/JCM.02573-06subject
Has Abstractpub_date
2007-09-01 00:00:00pages
3008-14issue
9eissn
0095-1137issn
1098-660Xpii
JCM.02573-06journal_volume
45pub_type
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