Evaluation of a PCR-based universal heteroduplex generator assay as a tool for rapid detection of multidrug-resistant Mycobacterium tuberculosis in Peru.

abstract：:This report describes a DNA amplification procedure for routine identification of heat-labile-toxin-producing Escherichia coli. Two oligonucleotide primers were used in a polymerase chain reaction procedure to amplify a highly conserved region of the A subunit of the heat-labile enterotoxin gene. Amplifications were d...

journal_title：Journal of clinical microbiology

authors： Victor T,du Toit R,van Zyl J,Bester AJ,van Helden PD

更新日期：1991-01-01 00:00:00

abstract：:Bordetella spp. cause respiratory tract diseases in warm-blooded animals. Only Bordetella bronchiseptica has been reported to cause bacteremia in humans, and this rare infection usually occurs with pneumonia in immunocompromised patients. We describe "Bordetella hinzii" bacteremia in an AIDS patient without a respirat...

journal_title：Journal of clinical microbiology

authors： Cookson BT,Vandamme P,Carlson LC,Larson AM,Sheffield JV,Kersters K,Spach DH

更新日期：1994-10-01 00:00:00

abstract：:We previously demonstrated that whole blood contains significantly more hepatitis C virus (HCV) RNA than plasma. To validate the whole-blood-based HCV RNA detection method, a prospective comparison of HCV RNA detection in whole blood and plasma from 50 patients with chronic liver disease was undertaken. Whole-blood an...

journal_title：Journal of clinical microbiology

authors： Stapleton JT,Klinzman D,Schmidt WN,Pfaller MA,Wu P,LaBrecque DR,Han Jq,Phillips MJ,Woolson R,Alden B

更新日期：1999-03-01 00:00:00

abstract：:Twenty-three young adult rhesus monkeys from China were evaluated for the presence of Helicobacter pylori. Gastric body and antral biopsy samples were tested for H. pylori by PCR analysis, culture, rapid urease testing, and histologic evaluation. Serologic testing to detect H. pylori immunoglobulin G (IgG) antibodies ...

journal_title：Journal of clinical microbiology

authors： Handt LK,Fox JG,Yan LL,Shen Z,Pouch WJ,Ngai D,Motzel SL,Nolan TE,Klein HJ

更新日期：1997-01-01 00:00:00

abstract：:By using a single synthetic oligonucleotide primer pair in the polymerase chain reaction, we amplified specific Shiga-like-toxin (SLT) gene segments from DNAs of 20 clinical Escherichia coli isolates, irrespective of whether they produce SLT-I, SLT-II, or heretofore uncategorized SLTs. These segments were not detectab...

journal_title：Journal of clinical microbiology

authors： Karch H,Meyer T

更新日期：1989-12-01 00:00:00

abstract：:One of the major unanswered questions regarding the presence of Listeria monocytogenes in foods is how many cells must be ingested in order to cause illness. To answer this question, studies were undertaken by using Macaca fascicularis (cynomolgus monkey) as an animal model. Healthy nonhuman primates were dosed with v...

journal_title：Journal of clinical microbiology

authors： Farber JM,Daley E,Coates F,Beausoleil N,Fournier J

更新日期：1991-11-01 00:00:00

abstract：:Mycobacterium bovis infection in wildlife and feral species is a potential source of infection for livestock and a threat to protected and endangered species. The aim of this study was to identify Spanish wild animal species infected with M. bovis through bacteriological culture and spacer oligonucleotide typing (spol...

journal_title：Journal of clinical microbiology

authors： Aranaz A,De Juan L,Montero N,Sánchez C,Galka M,Delso C,Alvarez J,Romero B,Bezos J,Vela AI,Briones V,Mateos A,Domínguez L

更新日期：2004-06-01 00:00:00

abstract：:Rapid detection of drug-resistant bacteria in clinical samples plays an instrumental role in patients' infection management and in implementing effective infection control policies. In the study described in this report, we validated a multiplex TaqMan real-time quantitative PCR (qPCR) assay for the detection of bla(K...

journal_title：Journal of clinical microbiology

authors： Hindiyeh M,Smollan G,Grossman Z,Ram D,Robinov J,Belausov N,Ben-David D,Tal I,Davidson Y,Shamiss A,Mendelson E,Keller N

更新日期：2011-07-01 00:00:00

abstract：:Explanted orthopedic implants from 54 patients with aseptic failure and 24 patients with prosthetic knee or hip infection were sonicated in polyethylene bags. The sensitivities of periprosthetic tissue and sonicate fluid cultures for the diagnosis of prosthetic joint infection were 54% and 75%, whereas the specificiti...

journal_title：Journal of clinical microbiology

authors： Trampuz A,Piper KE,Hanssen AD,Osmon DR,Cockerill FR,Steckelberg JM,Patel R

更新日期：2006-02-01 00:00:00

abstract：:Leptospira is a highly diverse genus comprising many species and serogroups in Brazil as well as all over the world. However, a study by arbitrarily primed PCR of 44 leptospiral strains isolated from humans during three different outbreaks in Brazilian urban centers reveals that 43 of 44 isolates exhibit very similar ...

journal_title：Journal of clinical microbiology

authors： Pereira MM,Matsuo MG,Bauab AR,Vasconcelos SA,Moraes ZM,Baranton G,Saint Girons I

更新日期：2000-01-01 00:00:00

abstract：:We have shown that a modification of the color-coded antigen developed by Kasatiya and Lambert (Appl. Microbiol. 28:317-318, 1974) can be used as a substitute for other nontreponemal antigens used in screening tests for syphilis. The antigen is based on the Venereal Disease Research Laboratory antigen, with EDTA, chol...

journal_title：Journal of clinical microbiology

authors： Pettit DE,Larsen SA,Harbec PS,Feeley JC,Parham CE,Cruce DD,Hambie EA,Perryman MW

更新日期：1983-11-01 00:00:00

abstract：:The Cobas-Bact (Roche Diagnostics, Basel, Switzerland) new rotor for the identification (ID) in 4 h 20 min of 33 members of the family Enterobacteriaceae to genus and species level was evaluated by testing 444 strains of which 398 belonged to common species and 46 belonged to rare species of Enterobacteriaceae. Each s...

journal_title：Journal of clinical microbiology

authors： Wenger A,Tissières LJ,Praplan FR,Kamm WR,Bille J

更新日期：1987-01-01 00:00:00

abstract：:Fungal infection of the thyroid is rare. Most reported cases have involved Aspergillus, Coccidioides, and Candida species in the setting of disseminated disease. Infection of the thyroid with Histoplasma capsulatum is rarely reported as part of disseminated disease, even in geographic areas where histoplasmosis is end...

journal_title：Journal of clinical microbiology

authors： Goldani LZ,Klock C,Diehl A,Monteiro AC,Maia AL

更新日期：2000-10-01 00:00:00

abstract：:To compare Simplexa Flu A/B & RSV PCR with cytospin-immunofluorescence and laboratory-developed TaqMan PCR methods, 445 nasopharyngeal samples were tested. Of these, 199 were positive (46 for respiratory syncytial virus [RSV], 120 for influenza A, and 33 for influenza B) and 246 were negative. The direct fluorescent-a...

journal_title：Journal of clinical microbiology

authors： Landry ML,Ferguson D

更新日期：2014-08-01 00:00:00

abstract：:In Mycobacterium smegmatis and a limited number of Mycobacterium tuberculosis strains, the involvement of alterations of the 16S rRNA gene (rrs) in resistance to kanamycin has been shown. To investigate the extent to which mutations in a specific region of the rrs gene and the kanamycin-resistant phenotype in clinical...

journal_title：Journal of clinical microbiology

authors： Suzuki Y,Katsukawa C,Tamaru A,Abe C,Makino M,Mizuguchi Y,Taniguchi H

更新日期：1998-05-01 00:00:00

abstract：:The DNA relatedness and restriction fragment length polymorphism patterns of whole-cell DNA and the general phenotypic properties of 14 isolates of the form species Candida parapsilosis representing three diverse genetic groups were compared. The data confirm the unrelatedness of the three groups at the species level....

journal_title：Journal of clinical microbiology

authors： Roy B,Meyer SA

更新日期：1998-01-01 00:00:00

abstract：:This three-center collaborative study was conducted to evaluate samples of the Microbial Profile System (MPS) antimicrobial microdilution panels [previously produced by Minnesota Mining & Manufacturing Co., (3M Co.), St. Paul, Minn. and currently produced by Flow Laboratories, Inc., Rockville, Md.). This was a three-p...

journal_title：Journal of clinical microbiology

authors： Gerlach EH,Jones RN,Barry AL

更新日期：1983-03-01 00:00:00

abstract：:We report an unusual case of culture-proven pneumococcal and meningococcal mixed meningitis in an 18-month-old girl. The patient responded well to antimicrobial therapy and recovered completely without sequelae. No underlying condition could be demonstrated except a rhinitis of unknown etiology 2 days before the onset...

journal_title：Journal of clinical microbiology

authors： Marchandin H,Ventura V,Alonso JM,Van de Perre P

更新日期：2005-03-01 00:00:00

abstract：:In a cohort of subjects from Italy, anti-hepatitis C virus (HCV) and HCV RNA [HCV(+) subgroup] prevalences were 24.6 and 79.6%, respectively. HCV types 1b and 2a/c accounted for 95% of infections. Adjusted alanine aminotransferase levels were higher in males than in females and in RNA-positive subjects than in RNA-neg...

journal_title：Journal of clinical microbiology

authors： Osella AR,Sonzogni L,Cavallini A,Foti L,Guerra V,Di Leo A,Mondelli MU,Misciagna G,Silini EM

更新日期：1999-07-01 00:00:00

abstract：:Time to detection (TTD) in automated blood culture systems is delayed for sensitive microorganisms in the presence of antimicrobial substances and has been associated with worse outcomes for sepsis patients on inadequate empirical therapy. While resin addition removes antimicrobial substances to various degrees from b...

journal_title：Journal of clinical microbiology

authors： Mitteregger D,Barousch W,Nehr M,Kundi M,Zeitlinger M,Makristathis A,Hirschl AM

更新日期：2013-05-01 00:00:00

abstract：:The capsular-like envelope of Legionella pneumophila strains Togus 1 (serotype 2) and Philadelphia 1 (serotype 1) was isolated and purified by column chromatography on Sepharose 6B. Antibody raised in rabbits to these two antigenic materials did not cross-react in gel diffusion. Upon electrophoresis followed by gel di...

journal_title：Journal of clinical microbiology

authors： Smith RA,DiGiorgio S,Darner J,Wilhelm A

更新日期：1981-04-01 00:00:00

abstract：:The current diagnosis of West Nile virus (WNV) infection is primarily based on serology, since molecular identification of WNV RNA is unreliable due to the short viremia and absence of detectable virus in cerebrospinal fluid (CSF). Recent studies have shown that WNV RNA can be detected in urine for a longer period and...

journal_title：Journal of clinical microbiology

authors： Lustig Y,Mannasse B,Koren R,Katz-Likvornik S,Hindiyeh M,Mandelboim M,Dovrat S,Sofer D,Mendelson E

更新日期：2016-09-01 00:00:00

abstract：:Detection of human papillomavirus (HPV) E6/E7 oncogene expression may be more predictive of cervical cancer risk than testing for HPV DNA. The Aptima HPV test (Gen-Probe) detects E6/E7 mRNA of 14 oncogenic types. Its clinical performance was compared with that of the Hybrid Capture 2 DNA test (HC2; Qiagen) in women re...

journal_title：Journal of clinical microbiology

authors： Ratnam S,Coutlee F,Fontaine D,Bentley J,Escott N,Ghatage P,Gadag V,Holloway G,Bartellas E,Kum N,Giede C,Lear A

更新日期：2011-02-01 00:00:00

abstract：:A variety of bacterins, vaccines, and antisera retained greater than 90% of their original level of mercurial preservative after lyophilization, and this might influence certain uses of these products. ...

journal_title：Journal of clinical microbiology

authors： Pemberton JR

更新日期：1975-12-01 00:00:00

abstract：:A dot immunobinding assay was used to detect antibodies to measles virus, mumps virus, and rubella virus antigens. Filter paper soaked with serum or whole blood was directly applied to the antigen-coated nitrocellulose sheets. The test was easy to perform, and its results agreed very well with those obtained by standa...

journal_title：Journal of clinical microbiology

authors： Condorelli F,Ziegler T

更新日期：1993-03-01 00:00:00

abstract：:A high-throughput multiplex bead suspension array was developed for the rapid subgenogrouping of EV71 strains, based on single nucleotide polymorphisms observed within the VP1 region with a high sensitivity as low as 1 PFU. Of 33 viral isolates and 55 clinical samples, all EV71 strains were successfully detected and c...

journal_title：Journal of clinical microbiology

authors： Wu Y,Tan EL,Yeo A,Chan KP,Nishimura H,Cardosa MJ,Poh CL,Quak SH,Chow VT

更新日期：2011-01-01 00:00:00

abstract：:Polyomavirus BK (BKV) causes asymptomatic latent infection in the human host that is reactivated during periods of immune suppression. Detection by conventional tube cell culture is difficult and time consuming because BKV exhibits slow growth with late (14 to 28 days) and subtle cytopathic effects. We developed a she...

journal_title：Journal of clinical microbiology

authors： Marshall WF,Telenti A,Proper J,Aksamit AJ,Smith TF

更新日期：1990-07-01 00:00:00

abstract：:This study assessed the diagnostic performance of the new COVID19SEROSpeed IgM/IgG rapid test (BioSpeedia, a spinoff of the Pasteur Institute of Paris) for the detection of antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in comparison to other commercial antibody assays through a large ...

journal_title：Journal of clinical microbiology

authors： Plebani M,Parčina M,Bechri I,Zehender G,Terkeš V,Abdel Hafith B,Antinori S,Pillet S,Gonzalo S,Hoerauf A,Lai A,Morović M,Bourlet T,Torre A,Pozzetto B,Galli M

更新日期：2021-01-21 00:00:00

abstract：:Clostridium difficile is the bacterium most commonly surmised to cause antimicrobial- and hospital-associated diarrhea in developed countries worldwide, and such infections are thought to be increasing in frequency and severity. A laboratory-based study was carried out to characterize C. difficile strains isolated fro...

journal_title：Journal of clinical microbiology

authors： Martin H,Willey B,Low DE,Staempfli HR,McGeer A,Boerlin P,Mulvey M,Weese JS

更新日期：2008-09-01 00:00:00

abstract：:Gram-negative, anaerobic bacilli are unusual organisms to be isolated in cases of acute septic arthritis. We report the isolation of Fusobacterium necrophorum from joint aspirate in a case of acute septic arthritis, which presented 3 weeks after the drainage of a dental abscess. ...

journal_title：Journal of clinical microbiology

authors： Sonsale PD,Philipson MR,Bowskill J

更新日期：2004-07-01 00:00:00