Mixed bacterial meningitis due to Streptococcus pneumoniae and Neisseria meningitidis in an 18-month-old child.

abstract：:Herpes simplex virus (HSV) causes acute and relapsing symptoms characterized by ulcerative lesions. Laboratory diagnosis of HSV in cutaneous or mucocutaneous lesions has historically been performed with the use of viral cell culture systems; however, these tests are laborious and suffer decreased sensitivity for advan...

journal_title：Journal of clinical microbiology

authors： Faron ML,Ledeboer NA,Patel A,Beqa SH,Yen-Lieberman B,Kohn D,Leber AL,Mayne D,Northern WI,Buchan BW

更新日期：2016-08-01 00:00:00

abstract：:The prevalence of Leishmania infantum-specific antibodies and asymptomatic infection was assessed in a randomized sample of 526 healthy adults from a continental area of northwestern Italy where L. infantum is not endemic and where autochthonous cases of visceral leishmaniasis (VL) were recently reported. L. infantum-...

journal_title：Journal of clinical microbiology

authors： Biglino A,Bolla C,Concialdi E,Trisciuoglio A,Romano A,Ferroglio E

更新日期：2010-01-01 00:00:00

abstract：:Antigenic sites on the nucleocapsid (N) protein of severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) were mapped by Pepscan analysis with overlapping peptides that span the N protein sequence. Two major immunodominant epitopes located in the C-terminal region (amino acids [aa] 362 to 412) and middle regi...

journal_title：Journal of clinical microbiology

authors： He Y,Zhou Y,Wu H,Kou Z,Liu S,Jiang S

更新日期：2004-11-01 00:00:00

abstract：:Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and conventional standard methods were compared for time to pathogen identification and impact on clinical outcomes in peritoneal dialysis-related peritonitis patients. The MALDI-TOF MS method identified the causative microorga...

journal_title：Journal of clinical microbiology

authors： Lin WH,Hwang JC,Tseng CC,Chang YT,Wu AB,Yan JJ,Wu JJ,Wang MC

更新日期：2016-05-01 00:00:00

abstract：:Of 522 patients with Propionibacterium acnes bacteremia (PAB), 18 (3.5%) had clinically significant PAB. Of these 18 patients, 10 (55.6%) had hospital-acquired bacteremia and 6 (33.3%) had undergone invasive procedures before development of PAB. One patient with a ventricular septal defect presented with infective end...

journal_title：Journal of clinical microbiology

authors： Park HJ,Na S,Park SY,Moon SM,Cho OH,Park KH,Chong YP,Kim SH,Lee SO,Kim YS,Woo JH,Kim MN,Choi SH

更新日期：2011-04-01 00:00:00

abstract：:A colony immunoblot method was developed for serotyping the capsular polysaccharides expressed by Staphylococcus aureus isolates. The method was rapid and specific and was performed with either polyclonal or monoclonal antibodies specific for each of the capsule types. S. aureus isolates were obtained from patients wi...

journal_title：Journal of clinical microbiology

authors： Lee JC,Liu MJ,Parsonnet J,Arbeit RD

更新日期：1990-12-01 00:00:00

abstract：:Vulvovaginal candidiasis (VVC) remains a common cause of morbidity, with three-quarters of women affected during their lifetimes. Use of antibiotics is an acknowledged trigger for VVC, which adversely affects women's physical and emotional health. Knowledge of patterns of genital Candida species-level identification i...

journal_title：Journal of clinical microbiology

authors： Pirotta MV,Garland SM

更新日期：2006-09-01 00:00:00

abstract：:Of the media examined, thioglycolate broth supplemented with 5% lysed sheep blood, Butzler antibiotic mixture, and 0.1% lauryl sulfate was the most sensitive enrichment medium for recovery of wild-type strains of Campylobacter jejuni from cecal contents of chickens and chicken livers. It allowed the retrieval of 1 CFU...

journal_title：Journal of clinical microbiology

authors： Barot MS,Bokkenheuser VD

更新日期：1984-07-01 00:00:00

abstract：:Human and porcine enterotoxigenic strains of Escherichia coli were cultivated in tryptone-yeast extract medium or brain heart infusion broth and tested for production of heat-labile enterotoxin by the GM1 ganglioside enzyme-linked immunosorbent assay (GM1-ELISA) and the Y1 adrenal cell assay. When testing for enteroto...

journal_title：Journal of clinical microbiology

authors： Gustafsson B,Möllby R

更新日期：1982-02-01 00:00:00

abstract：:We describe a multiplex reverse transcription-PCR (m-RT-PCR) assay that is able to detect and differentiate all known human parainfluenza viruses (HPIVs). Serial dilution experiments with reference strains that compared cell culture isolation and m-RT-PCR showed sensitivities ranging from 0.0004 50% tissue culture inf...

journal_title：Journal of clinical microbiology

authors： Aguilar JC,Pérez-Breña MP,García ML,Cruz N,Erdman DD,Echevarría JE

更新日期：2000-03-01 00:00:00

abstract：:Astrovirus infections were detected by enzyme immunoassay in 12 (5%) of 251 stool samples from children with gastroenteritis from Bogota, Colombia. In addition, astroviruses were detected by reverse transcription-PCR in 3 (10%) of 29 stool samples negative for other enteric pathogens collected in Caracas, Venezuela, f...

journal_title：Journal of clinical microbiology

authors： Medina SM,Gutierrez MF,Liprandi F,Ludert JE

更新日期：2000-09-01 00:00:00

abstract：:New variants of the influenza A(H1N1)pdm09 and A(H3N2) viruses were detected in Taiwan between 2012 and 2013. Some of these variants were not detected in clinical specimens using a common real-time reverse transcription-PCR (RT-PCR) assay that targeted the conserved regions of the viral matrix (M) genes. An analysis o...

journal_title：Journal of clinical microbiology

authors： Yang JR,Kuo CY,Huang HY,Wu FT,Huang YL,Cheng CY,Su YT,Chang FY,Wu HS,Liu MT

更新日期：2014-01-01 00:00:00

abstract：:Acinetobacter isolates associated with casualties from the Iraq conflict from the United States were compared with those from the United Kingdom by pulsed-field gel electrophoresis and integron analysis. Representatives of the main outbreak strain associated with casualties from both countries were indistinguishable i...

journal_title：Journal of clinical microbiology

authors： Turton JF,Kaufmann ME,Gill MJ,Pike R,Scott PT,Fishbain J,Craft D,Deye G,Riddell S,Lindler LE,Pitt TL

更新日期：2006-07-01 00:00:00

abstract：:Two national surveys were conducted to describe the incidence and prevalence of Enterobacter aerogenes in 21 Belgian hospitals in 1996 and 1997 and to characterize the genotypic diversity and the antimicrobial resistance profiles of clinical strains of E. aerogenes isolated from hospitalized patients in Belgium in 199...

journal_title：Journal of clinical microbiology

authors： De Gheldre Y,Struelens MJ,Glupczynski Y,De Mol P,Maes N,Nonhoff C,Chetoui H,Sion C,Ronveaux O,Vaneechoutte M,Groupement pour le Dépistage, l'Etude et la Prevention des Infections Hospitalières (GDEPIH-GOSPIZ).

更新日期：2001-03-01 00:00:00

abstract：:In order to increase the application potential of the direct agglutination test (DAT) for the detection of anti-Leishmania antibodies in human serum samples, we developed an antigen based on stained and freeze-dried Leishmania donovani promastigotes. We describe here the evaluation of the performance of the DAT based ...

journal_title：Journal of clinical microbiology

authors： Meredith SE,Kroon NC,Sondorp E,Seaman J,Goris MG,van Ingen CW,Oosting H,Schoone GJ,Terpstra WJ,Oskam L

更新日期：1995-07-01 00:00:00

abstract：:This report presents the case of a patient with a massive pyelonephritis and a urosepsis caused by Streptococcus pneumoniae. This case is unusual as the focus was distant from the respiratory tract, the usual primary site of infection caused by this organism. No other primary site of infection was documented. ...

journal_title：Journal of clinical microbiology

authors： Dufke S,Kunze-Kronawitter H,Schubert S

更新日期：2004-09-01 00:00:00

abstract：:Escherichia coli is the most common cause of human and canine urinary tract infection (UTI). Clonal groups, often with high levels of antimicrobial resistance, are a major component of the E. coli population that causes human UTI. While little is known about the population structure of E. coli that causes UTI in dogs,...

journal_title：Journal of clinical microbiology

authors： LeCuyer TE,Byrne BA,Daniels JB,Diaz-Campos DV,Hammac GK,Miller CB,Besser TE,Davis MA

更新日期：2018-08-27 00:00:00

abstract：:Broad-spectrum analysis for pathogens in patients with respiratory tract infections is becoming more relevant as the number of potential infectious agents is still increasing. Here we describe the new multiparameter RespiFinder assay, which is based on the multiplex ligation-dependent probe amplification (MLPA) techno...

journal_title：Journal of clinical microbiology

authors： Reijans M,Dingemans G,Klaassen CH,Meis JF,Keijdener J,Mulders B,Eadie K,van Leeuwen W,van Belkum A,Horrevorts AM,Simons G

更新日期：2008-04-01 00:00:00

abstract：:Conventional culture techniques are limited in the ability to detect multiple Streptococcus pneumoniae serotypes in nasopharyngeal (NP) secretions. We developed an immunoblot (IB) method with monoclonal antibodies (MAbs) to detect S. pneumoniae and to identify serotypes. NP specimens stored in skim milk-tryptone-gluco...

journal_title：Journal of clinical microbiology

authors： Bronsdon MA,O'Brien KL,Facklam RR,Whitney CG,Schwartz B,Carlone GM

更新日期：2004-04-01 00:00:00

abstract：:We report a case of catheter-related bloodstream infection by Tsukamurella inchonensis, identified using 16S rRNA gene sequencing, in a patient with myelofibrosis who underwent a bone marrow transplant. Tsukamurella species infections are rare. To our knowledge, this is the first case of T. inchonensis bloodstream inf...

journal_title：Journal of clinical microbiology

authors： Takebe I,Sawabe E,Ohkusu K,Tojo N,Tohda S

更新日期：2014-06-01 00:00:00

abstract：:Clinical evidence for failure with beta-lactam therapy has been lacking for patients with borderline oxacillin-resistant Staphylococcus aureus (BORSA) infections. We describe a failure of cloxacillin for a patient with endocarditis due to BORSA. The isolate also had false-negative thermonuclease and coagulase test res...

journal_title：Journal of clinical microbiology

authors： Skinner S,Murray M,Walus T,Karlowsky JA

更新日期：2009-03-01 00:00:00

abstract：:Porcine reproductive and respiratory syndrome has been devastating the swine industry since the late 1980s. The disease has been controlled, to some extent, through the use of modified live-attenuated (MLV) vaccines once available. However, such a practice periodically resulted in isolation or detection of vaccine-lik...

journal_title：Journal of clinical microbiology

authors： Key KF,Guenette DK,Yoon KJ,Halbur PG,Toth TE,Meng XJ

更新日期：2003-06-01 00:00:00

abstract：:The pandemic spread of Vibrio parahaemolyticus is an international public health issue. Because of the outbreak potential of the organism, it is critical to establish an internationally recognized molecular subtyping protocol for V. parahaemolyticus that is both rapid and robust as a means to monitor its further sprea...

journal_title：Journal of clinical microbiology

authors： Kam KM,Luey CK,Parsons MB,Cooper KL,Nair GB,Alam M,Islam MA,Cheung DT,Chu YW,Ramamurthy T,Pazhani GP,Bhattacharya SK,Watanabe H,Terajima J,Arakawa E,Ratchtrachenchai OA,Huttayananont S,Ribot EM,Gerner-Smidt P,Swamin

更新日期：2008-08-01 00:00:00

abstract：:Candida dubliniensis is often associated with C. albicans in cultures. Easy-to-perform selective isolation procedures for these closely related species do not exist. Therefore, we evaluated previously described discriminatory phenotypic markers for C. dubliniensis. A total of 150 oral rinses from human immunodeficienc...

journal_title：Journal of clinical microbiology

authors： Tintelnot K,Haase G,Seibold M,Bergmann F,Staemmler M,Franz T,Naumann D

更新日期：2000-04-01 00:00:00

abstract：:A case of tick-borne encephalitis (TBE) has not been reported for many years in Japan, although a serological survey of sera from domestic animals suggested the presence of TBE foci in Hokkaido, the northern island of Japan. Studies were conducted to prove the presence of an endemic focus of TBE virus in Japan by mean...

journal_title：Journal of clinical microbiology

authors： Takashima I,Morita K,Chiba M,Hayasaka D,Sato T,Takezawa C,Igarashi A,Kariwa H,Yoshimatsu K,Arikawa J,Hashimoto N

更新日期：1997-08-01 00:00:00

abstract：:In recent years testing responsibilities for high-consequence pathogens have been expanded from national reference laboratories into networks of local and regional laboratories in order to support enhanced disease surveillance and to test for surge capacity. This movement of testing of select agents and high-consequen...

journal_title：Journal of clinical microbiology

authors： Hietala SK,Crossley BM

更新日期：2006-01-01 00:00:00

abstract：:Several studies have identified nonsterile blood collection tubes as the reservoir for contaminating microorganisms in outbreaks of pseudobacteremia. Microorganisms are thought to be transferred from the nonsterile tubes to the blood culture tubes when blood is drawn for cultures, but the validity of this hypothesis h...

journal_title：Journal of clinical microbiology

authors： McNeil MM,Davis BJ,Anderson RL,Martone WJ,Solomon SL

更新日期：1985-07-01 00:00:00

abstract：:The recovery of Mycobacterium tuberculosis from sputa positive or negative for acid-fast bacilli that were stored for 17 +/- 7 days and inoculated in the BACTEC MGIT 960 system (MGIT) was higher than that from sputa inoculated in Lowenstein-Jensen medium. MGIT is useful for isolation of M. tuberculosis from sputa subj...

journal_title：Journal of clinical microbiology

authors： Pardini M,Varaine F,Bonnet M,Orefici G,Oggioni MR,LONG-DRUG Study Group.,Fattorini L

更新日期：2007-02-01 00:00:00

abstract：:Large numbers of Mycobacterium tuberculosis isolates that were obtained from patients' sputa on diagnosis and during follow-up after short-course chemotherapy in Madras, India, have either no copy or only a single copy of IS6110. This poses a limitation for DNA fingerprinting with an IS6110-based probe to determine th...

journal_title：Journal of clinical microbiology

authors： Sahadevan R,Narayanan S,Paramasivan CN,Prabhakar R,Narayanan PR

更新日期：1995-11-01 00:00:00

abstract：:An optimized reverse transcription (RT)-PCR protocol for the intravitam detection of rabies virus genomic RNA was tested with clinical samples obtained from 28 patients suspected of having rabies, 9 of whom were confirmed to have had rabies by postmortem examination. RT-PCR using saliva combined with an immunofluoresc...

journal_title：Journal of clinical microbiology

authors： Crepin P,Audry L,Rotivel Y,Gacoin A,Caroff C,Bourhy H

更新日期：1998-04-01 00:00:00