Sonication of explanted prosthetic components in bags for diagnosis of prosthetic joint infection is associated with risk of contamination.

Abstract:

:Explanted orthopedic implants from 54 patients with aseptic failure and 24 patients with prosthetic knee or hip infection were sonicated in polyethylene bags. The sensitivities of periprosthetic tissue and sonicate fluid cultures for the diagnosis of prosthetic joint infection were 54% and 75%, whereas the specificities were 98% and 87%, respectively. Sonication in bags improved bacterial recovery from the surface of orthopedic implants; however, it lacked specificity, due to bag leakage.

journal_name

J Clin Microbiol

authors

Trampuz A,Piper KE,Hanssen AD,Osmon DR,Cockerill FR,Steckelberg JM,Patel R

doi

10.1128/JCM.44.2.628-631.2006

subject

Has Abstract

pub_date

2006-02-01 00:00:00

pages

628-31

issue

2

eissn

0095-1137

issn

1098-660X

pii

44/2/628

journal_volume

44

pub_type

杂志文章
  • Mutanolysin enhancement of serogrouping of single colonies of streptococci.

    abstract::Single colonies of beta-hemolytic streptococci could be grouped by antibody-coated latex bead agglutination or coagglutination with staphylococci coated with antibody after incubation of the colonies with mutanolysin. This simple and quick procedure provided an enzymatic means of enhancing the sensitivity of tests suc...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.15.1.184-186.1982

    authors: Calandra GB,Henson CL

    更新日期:1982-01-01 00:00:00

  • Aerococcus-like organism, a newly recognized potential urinary tract pathogen.

    abstract::During the last 10 to 15 years, Aerococcus-like organisms have been isolated from urinary tract specimens from hospitalized patients at Bispebjerg Hospital, Copenhagen, Denmark, with signs of urinary tract infection, and a single strain has been deposited at the National Collection of Type Cultures (NCTC 12142). An ad...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.29.5.1049-1053.1991

    authors: Christensen JJ,Vibits H,Ursing J,Korner B

    更新日期:1991-05-01 00:00:00

  • New microassay for quantitation of endotoxin using Limulus amebocyte lysate combined with enzyme-linked immunosorbent assay.

    abstract::A combined method of the Limulus amebocyte lysate (LAL) test and the enzyme-linked immunosorbent assay for quantitation of endotoxin was developed based on our observation that the antigenicity of coagulogen, a major protein in LAL, was lost when LAL reacted with endotoxin as shown by immunoblotting. Determination of ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.26.8.1464-1470.1988

    authors: Zhang GH,Baek L,Koch C

    更新日期:1988-08-01 00:00:00

  • Comparison of indirect hemagglutination and indirect immunofluorescence tests with microneutralization tests for detection of type-specific Herpesvirus hominis antibody.

    abstract::Indirect hemagglutinating and immunofluorescent antibody responses to Herpesvirus hominis types 1 and 2 were compared to neutralizing antibody responses in infected humans from whom H. hominis type 1 or 2 was isolated. The indirect immunofluorescent antibody test was shown to be the most sensitive and specific for pri...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:

    authors: Johnson LD,Fuccillo DA,Stalder H,Oxman MA,Fraser CE,Madden DL

    更新日期:1979-03-01 00:00:00

  • Radial passive immune hemolysis assay for detection of heat-labile enterotoxin produced by individual colonies of Escherichia coli or Vibrio cholerae.

    abstract::A rapid screening test has been developed to detect heat-labile enterotoxin produced by individual colonies of Escherichia coli or Vibrio cholerae growing on media solidified with agar. The applicability of this method for isolating tox mutants of E. coli and V. cholerae has been demonstrated. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:

    authors: Bramucci MG,Holmes RK

    更新日期:1978-08-01 00:00:00

  • Aeration selects for mucoid phenotype of Pseudomonas aeruginosa.

    abstract::A mucoid strain of Pseudomonas aeruginosa isolated from a patient with cystic fibrosis and its nonmucoid revertant were grown in a chemically defined alginate-promoting medium under batch and continuous culture conditions. Selection for the mucoid and nonmucoid phenotype was accomplished by varying the levels of air a...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.24.6.986-990.1986

    authors: Krieg DP,Bass JA,Mattingly SJ

    更新日期:1986-12-01 00:00:00

  • Use of a signature nucleotide sequence of hepatitis C virus for detection of viral RNA in human serum and plasma.

    abstract::The nucleic acid sequence of the putative 5'-untranslated (5PUT) region of hepatitis C virus (HCV), determined for samples obtained from a variety of geographic origins, was found to be over 98% conserved among all isolates. On the basis of this signature sequence for HCV, a viral RNA assay was developed by using cDNA...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.29.11.2528-2534.1991

    authors: Cha TA,Kolberg J,Irvine B,Stempien M,Beall E,Yano M,Choo QL,Houghton M,Kuo G,Han JH

    更新日期:1991-11-01 00:00:00

  • Francisella tularensis Periprosthetic Joint Infections Diagnosed with Growth in Cultures.

    abstract::Tularemia caused by Francisella tularensis is a zoonotic infection of the Northern Hemisphere that mainly affects the skin, lymph nodes, bloodstream, and lungs. Other manifestations of tularemia are very rare, especially those with musculoskeletal involvement. Presenting in 2016, we diagnosed two cases of periprosthet...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,评审

    doi:10.1128/JCM.00339-19

    authors: Chrdle A,Trnka T,Musil D,Fucentese SF,Bode P,Keller PM,Achermann Y

    更新日期:2019-07-26 00:00:00

  • Use of Haemophilus test medium for broth microdilution antimicrobial susceptibility testing of Streptococcus pneumoniae.

    abstract::A recently described medium (Haemophilus test medium [HTM]) for antimicrobial susceptibility testing of Haemophilus influenzae was evaluated in this study for broth microdilution testing of Streptococcus pneumoniae. A total of 137 clinical isolates was tested against 11 antimicrobial agents, using Mueller-Hinton broth...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.28.3.430-434.1990

    authors: Jorgensen JH,Maher LA,Howell AW

    更新日期:1990-03-01 00:00:00

  • Practical colorimeter for direct measurement of microplates in enzyme immunoassay systems.

    abstract::A colorimeter capable of measuring results of enzyme-linked immunosorbent assay (ELISA) reactions directly in the wells of a microtiter plate is described. This colorimeter proved to be as accurate as a conventional spectrophotometer in assessing ELISA reactions, but had the advantage of not requiring transfer of the ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:

    authors: Clem TR,Yolken RH

    更新日期:1978-01-01 00:00:00

  • Multicenter Assessment of Gram Stain Error Rates.

    abstract::Gram stains remain the cornerstone of diagnostic testing in the microbiology laboratory for the guidance of empirical treatment prior to availability of culture results. Incorrectly interpreted Gram stains may adversely impact patient care, and yet there are no comprehensive studies that have evaluated the reliability...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,多中心研究

    doi:10.1128/JCM.03066-15

    authors: Samuel LP,Balada-Llasat JM,Harrington A,Cavagnolo R

    更新日期:2016-06-01 00:00:00

  • Development of multiplex PCRs for detection of common viral pathogens and agents of congenital infections.

    abstract::Potential causes of congenital infection include Toxoplasma gondii and viruses such as cytomegalovirus (CMV), enterovirus, hepatitis C virus, herpes simplex virus types 1 and 2 (HSV-1 and -2), human herpesvirus types 6, 7, and 8, lymphocytic choriomeningitis virus, parvovirus, rubella virus, and varicella-zoster virus...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.43.10.5102-5110.2005

    authors: McIver CJ,Jacques CF,Chow SS,Munro SC,Scott GM,Roberts JA,Craig ME,Rawlinson WD

    更新日期:2005-10-01 00:00:00

  • New PCR primer pairs specific for Cryptococcus neoformans serotype A or B prepared on the basis of random amplified polymorphic DNA fingerprint pattern analyses.

    abstract::Thirty-three strains of Cryptococcus neoformans were isolated from clinical specimens, including specimens from AIDS patients in Brazil, and were classified into two serotypes; we detected 31 and 2 strains of serotypes A and B, respectively. Random amplified polymorphic DNA (RAPD) fingerprint pattern analyses of these...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.37.2.315-320.1999

    authors: Aoki FH,Imai T,Tanaka R,Mikami Y,Taguchi H,Nishimura NF,Nishimura K,Miyaji M,Schreiber AZ,Branchini ML

    更新日期:1999-02-01 00:00:00

  • Intestinal colonization of symptomatic and asymptomatic schoolchildren with Blastocystis hominis.

    abstract::A study of single stool specimens was done to determine the prevalence of intestinal parasites among 1,000 primary school children. A questionnaire was completed by each child's parents. Specimens were examined by using wet-mount preparation, formaline-ether concentration, and Sheather's flotation technique. Trichrome...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.32.11.2865-2866.1994

    authors: Nimri L,Batchoun R

    更新日期:1994-11-01 00:00:00

  • Modified coagglutination procedure for the serological grouping of streptococci.

    abstract::Cowan I staphylococci coated with antisera to streptococcal groups A, B, C, D, F, and G were used as coagglutination reagents in a modified coagglutination procedure (MCAP). Streptococcal group antigens were extracted with a Streptomyces albus-lysozyme enzyme mixture for 30 min at 55 degrees C and centrifuged, and the...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:

    authors: Carlson JR,McCarthy LR

    更新日期:1979-03-01 00:00:00

  • Identification of nocardia species by restriction endonuclease analysis of an amplified portion of the 16S rRNA gene.

    abstract::Identification of clinical isolates of Nocardia to the species level is important for defining the spectrum of disease produced by each species and for predicting antimicrobial susceptibility. We evaluated the usefulness of PCR amplification of a portion of the Nocardia 16S rRNA gene and subsequent restriction endonuc...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:

    authors: Conville PS,Fischer SH,Cartwright CP,Witebsky FG

    更新日期:2000-01-01 00:00:00

  • Detection of Streptococcus pneumoniae and Haemophilus influenzae type b antigens in acute nonbacteremic pneumonia.

    abstract::Commercially available latex agglutination and coagglutination reagents were evaluated for their ability to detect bacterial antigens in the sera of 165 patients to determine their suitability for rapid diagnosis of pneumonia. These reagents were used to detect the polysaccharide capsular antigens of Haemophilus influ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.25.2.248-250.1987

    authors: Martin SJ,Hoganson DA,Thomas ET

    更新日期:1987-02-01 00:00:00

  • Recovery of Borrelia burgdorferi by filtration.

    abstract::Bacterial contamination frequently interferes with successful recovery of the Lyme spirochete from cultures of tissue from Borrelia burgdorferi-infected humans, rodents, or ticks. We used 0.20- and 0.45-microns-pore-size syringe-tip filters to recover spirochetes from cultures contaminated with other bacteria. Low con...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.31.7.1896-1898.1993

    authors: Jobe DA,Callister SM,Schell RF

    更新日期:1993-07-01 00:00:00

  • Detection and quantification of infectious hypodermal and hematopoietic necrosis virus and white spot virus in shrimp using real-time quantitative PCR and SYBR Green chemistry.

    abstract::A rapid and highly sensitive real-time PCR detection and quantification method for infectious hypodermal and hematopoietic necrosis virus (IHHNV), a single-stranded DNA virus, and white spot virus (WSV), a double-stranded DNA (dsDNA) virus infecting penaeid shrimp (Penaeus sp.), was developed using the GeneAmp 5700 se...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.39.8.2835-2845.2001

    authors: Dhar AK,Roux MM,Klimpel KR

    更新日期:2001-08-01 00:00:00

  • Bioterrorism: a laboratory who does it?

    abstract::In October 2001, the first disseminated biological warfare attack was perpetrated on American soil. Initially, a few clinical microbiology laboratories were testing specimens from acutely ill patients and also being asked to test nasal swabs from the potentially exposed. Soon after, a significant number of clinical mi...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,评审

    doi:10.1128/JCM.00359-14

    authors: Craft DW,Lee PA,Rowlinson MC

    更新日期:2014-07-01 00:00:00

  • Rapid molecular detection of extrapulmonary tuberculosis by the automated GeneXpert MTB/RIF system.

    abstract::In total, 521 nonrespiratory specimens (91 urine, 30 gastric aspirate, 245 tissue, 113 pleural fluid, 19 cerebrospinal fluid [CSF], and 23 stool specimens) submitted to the German National Reference Laboratory for Mycobacteria (NRL) from May 2009 to August 2010 were comparatively investigated with the new molecular-ba...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02268-10

    authors: Hillemann D,Rüsch-Gerdes S,Boehme C,Richter E

    更新日期:2011-04-01 00:00:00

  • Characterization of Hafnia alvei by biochemical tests, random amplified polymorphic DNA PCR, and partial sequencing of 16S rRNA gene.

    abstract::Hafnia alvei strains which possess the attachment-effacement gene (eaeA) may have clinical importance as new diarrhea-causing pathogens and should therefore be differentiated from other H. alvei strains. We characterized diarrheal H. alvei strains, which were positive in the PCR test for the eaeA gene, using biochemic...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.33.9.2372-2376.1995

    authors: Ridell J,Siitonen A,Paulin L,Lindroos O,Korkeala H,Albert MJ

    更新日期:1995-09-01 00:00:00

  • Rapid detection and identification of Aspergillus from lower respiratory tract specimens by use of a combined probe-high-resolution melting analysis.

    abstract::Diagnosis of invasive aspergillosis (IA) requires increasingly rapid molecular methods that enable sensitive detection and discrimination between species. We designed and evaluated a real-time PCR-based method that combined melting temperature (T(m)) calling analysis of a specific probe with high-resolution melting an...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00176-12

    authors: Alonso M,Escribano P,Guinea J,Recio S,Simon A,Peláez T,Bouza E,García de Viedma D

    更新日期:2012-10-01 00:00:00

  • Evaluation of the sensitivities of DNA extraction and PCR methods for detection of Giardia duodenalis in stool specimens.

    abstract::Sensitivities of DNA extraction methods and PCR methods for Giardia duodenalis were evaluated. A combination of the most sensitive methods, i.e., FTA filter paper and a PCR protocol using RH11/RH4 and GiarF/GiarR primers, showed no significant differences compared to immunofluorescence assay in terms of their sensitiv...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01823-06

    authors: Nantavisai K,Mungthin M,Tan-ariya P,Rangsin R,Naaglor T,Leelayoova S

    更新日期:2007-02-01 00:00:00

  • Normalized enzyme-linked immunosorbent assay for determining immunoglobulin G antibodies to cytomegalovirus.

    abstract::A normalized enzyme-linked immunosorbent assay for the determination of immunoglobulin G antibodies to cytomegalovirus is described. The rapid assay involves three 30-min incubations and permits the quantitation of antibody levels with a single-specimen dilution in conjunction with a reference antibody preparation. Th...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.18.1.33-39.1983

    authors: Kiefer DJ,Phelps DA,Halbert SP

    更新日期:1983-07-01 00:00:00

  • Genetic heterogeneity among strains of Treponema phagedenis-like spirochetes isolated from dairy cattle with papillomatous digital dermatitis in Japan.

    abstract::Papillomatous digital dermatitis (PDD) is an infectious foot disease of cattle that is prevalent throughout the world. Although it has been prevalent in Japan since the first case was reported in 1992, full epidemiological and bacteriological examinations have not been conducted. We collected 91 lesions of PDD from 80...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01574-08

    authors: Yano T,Yamagami R,Misumi K,Kubota C,Moe KK,Hayashi T,Yoshitani K,Ohtake O,Misawa N

    更新日期:2009-03-01 00:00:00

  • Sequence variation in the outer membrane protein-encoding gene cmeC, conferring multidrug resistance among Campylobacter jejuni and Campylobacter coli strains isolated from different hosts.

    abstract::A novel PCR primer pair was used to detect the presence of cmeC in 131 Campylobacter jejuni and C. coli strains isolated from various hosts (cattle, turkeys, humans, and pigs). DNA sequence analysis revealed a high degree of genetic variation between the two species, while extremely limited genetic variation among iso...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01208-07

    authors: Fakhr MK,Logue CM

    更新日期:2007-10-01 00:00:00

  • Staphylococcal cassette chromosome mec and Panton-Valentine leukocidin characterization of methicillin-resistant Staphylococcus aureus clones.

    abstract::Staphylococcal cassette chromosome mec (SCCmec) types and Panton-Valentine leukocidin (PVL) gene carriage were compared among suspected community-associated methicillin-resistant Staphylococcus aureus MRSA (CA-MRSA) and health care-associated MRSA (HA-MRSA) isolates. CA-MRSA isolates carried the SCCmec type IV complex...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01706-06

    authors: Moroney SM,Heller LC,Arbuckle J,Talavera M,Widen RH

    更新日期:2007-03-01 00:00:00

  • Endocarditis caused by Capnocytophaga ochracea.

    abstract::Capnocytophaga ochracea is a gram-negative, fusiform bacillus which is part of the normal human oral flora. This organism is often isolated from periodontal lesions of patients with periodontitis and is associated with sepsis in granulocytopenic patients. We report here a case of endocarditis caused by C. ochracea. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.26.5.1061-1062.1988

    authors: Buu-Hoi AY,Joundy S,Acar JF

    更新日期:1988-05-01 00:00:00

  • Identification of urovirulence traits in Escherichia coli by comparison of urinary and rectal E. coli isolates from dogs with urinary tract infection.

    abstract::Spontaneously occurring urinary tract infection (UTI) in dogs was exploited as an experiment of nature to gain insights into UTI pathogenesis in humans. Concurrent urinary and rectal Escherichia coli isolates from 37 dogs with UTI were compared with respect to phylogenetic background, O antigens, and extended virulenc...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.41.1.337-345.2003

    authors: Johnson JR,Kaster N,Kuskowski MA,Ling GV

    更新日期:2003-01-01 00:00:00