Abstract:
:Explanted orthopedic implants from 54 patients with aseptic failure and 24 patients with prosthetic knee or hip infection were sonicated in polyethylene bags. The sensitivities of periprosthetic tissue and sonicate fluid cultures for the diagnosis of prosthetic joint infection were 54% and 75%, whereas the specificities were 98% and 87%, respectively. Sonication in bags improved bacterial recovery from the surface of orthopedic implants; however, it lacked specificity, due to bag leakage.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Trampuz A,Piper KE,Hanssen AD,Osmon DR,Cockerill FR,Steckelberg JM,Patel Rdoi
10.1128/JCM.44.2.628-631.2006subject
Has Abstractpub_date
2006-02-01 00:00:00pages
628-31issue
2eissn
0095-1137issn
1098-660Xpii
44/2/628journal_volume
44pub_type
杂志文章abstract::Single colonies of beta-hemolytic streptococci could be grouped by antibody-coated latex bead agglutination or coagglutination with staphylococci coated with antibody after incubation of the colonies with mutanolysin. This simple and quick procedure provided an enzymatic means of enhancing the sensitivity of tests suc...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.15.1.184-186.1982
更新日期:1982-01-01 00:00:00
abstract::During the last 10 to 15 years, Aerococcus-like organisms have been isolated from urinary tract specimens from hospitalized patients at Bispebjerg Hospital, Copenhagen, Denmark, with signs of urinary tract infection, and a single strain has been deposited at the National Collection of Type Cultures (NCTC 12142). An ad...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.29.5.1049-1053.1991
更新日期:1991-05-01 00:00:00
abstract::A combined method of the Limulus amebocyte lysate (LAL) test and the enzyme-linked immunosorbent assay for quantitation of endotoxin was developed based on our observation that the antigenicity of coagulogen, a major protein in LAL, was lost when LAL reacted with endotoxin as shown by immunoblotting. Determination of ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.26.8.1464-1470.1988
更新日期:1988-08-01 00:00:00
abstract::Indirect hemagglutinating and immunofluorescent antibody responses to Herpesvirus hominis types 1 and 2 were compared to neutralizing antibody responses in infected humans from whom H. hominis type 1 or 2 was isolated. The indirect immunofluorescent antibody test was shown to be the most sensitive and specific for pri...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:
更新日期:1979-03-01 00:00:00
abstract::A rapid screening test has been developed to detect heat-labile enterotoxin produced by individual colonies of Escherichia coli or Vibrio cholerae growing on media solidified with agar. The applicability of this method for isolating tox mutants of E. coli and V. cholerae has been demonstrated. ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:
更新日期:1978-08-01 00:00:00
abstract::A mucoid strain of Pseudomonas aeruginosa isolated from a patient with cystic fibrosis and its nonmucoid revertant were grown in a chemically defined alginate-promoting medium under batch and continuous culture conditions. Selection for the mucoid and nonmucoid phenotype was accomplished by varying the levels of air a...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.24.6.986-990.1986
更新日期:1986-12-01 00:00:00
abstract::The nucleic acid sequence of the putative 5'-untranslated (5PUT) region of hepatitis C virus (HCV), determined for samples obtained from a variety of geographic origins, was found to be over 98% conserved among all isolates. On the basis of this signature sequence for HCV, a viral RNA assay was developed by using cDNA...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.29.11.2528-2534.1991
更新日期:1991-11-01 00:00:00
abstract::Tularemia caused by Francisella tularensis is a zoonotic infection of the Northern Hemisphere that mainly affects the skin, lymph nodes, bloodstream, and lungs. Other manifestations of tularemia are very rare, especially those with musculoskeletal involvement. Presenting in 2016, we diagnosed two cases of periprosthet...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章,评审
doi:10.1128/JCM.00339-19
更新日期:2019-07-26 00:00:00
abstract::A recently described medium (Haemophilus test medium [HTM]) for antimicrobial susceptibility testing of Haemophilus influenzae was evaluated in this study for broth microdilution testing of Streptococcus pneumoniae. A total of 137 clinical isolates was tested against 11 antimicrobial agents, using Mueller-Hinton broth...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.28.3.430-434.1990
更新日期:1990-03-01 00:00:00
abstract::A colorimeter capable of measuring results of enzyme-linked immunosorbent assay (ELISA) reactions directly in the wells of a microtiter plate is described. This colorimeter proved to be as accurate as a conventional spectrophotometer in assessing ELISA reactions, but had the advantage of not requiring transfer of the ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:
更新日期:1978-01-01 00:00:00
abstract::Gram stains remain the cornerstone of diagnostic testing in the microbiology laboratory for the guidance of empirical treatment prior to availability of culture results. Incorrectly interpreted Gram stains may adversely impact patient care, and yet there are no comprehensive studies that have evaluated the reliability...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章,多中心研究
doi:10.1128/JCM.03066-15
更新日期:2016-06-01 00:00:00
abstract::Potential causes of congenital infection include Toxoplasma gondii and viruses such as cytomegalovirus (CMV), enterovirus, hepatitis C virus, herpes simplex virus types 1 and 2 (HSV-1 and -2), human herpesvirus types 6, 7, and 8, lymphocytic choriomeningitis virus, parvovirus, rubella virus, and varicella-zoster virus...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.43.10.5102-5110.2005
更新日期:2005-10-01 00:00:00
abstract::Thirty-three strains of Cryptococcus neoformans were isolated from clinical specimens, including specimens from AIDS patients in Brazil, and were classified into two serotypes; we detected 31 and 2 strains of serotypes A and B, respectively. Random amplified polymorphic DNA (RAPD) fingerprint pattern analyses of these...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.37.2.315-320.1999
更新日期:1999-02-01 00:00:00
abstract::A study of single stool specimens was done to determine the prevalence of intestinal parasites among 1,000 primary school children. A questionnaire was completed by each child's parents. Specimens were examined by using wet-mount preparation, formaline-ether concentration, and Sheather's flotation technique. Trichrome...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.32.11.2865-2866.1994
更新日期:1994-11-01 00:00:00
abstract::Cowan I staphylococci coated with antisera to streptococcal groups A, B, C, D, F, and G were used as coagglutination reagents in a modified coagglutination procedure (MCAP). Streptococcal group antigens were extracted with a Streptomyces albus-lysozyme enzyme mixture for 30 min at 55 degrees C and centrifuged, and the...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:
更新日期:1979-03-01 00:00:00
abstract::Identification of clinical isolates of Nocardia to the species level is important for defining the spectrum of disease produced by each species and for predicting antimicrobial susceptibility. We evaluated the usefulness of PCR amplification of a portion of the Nocardia 16S rRNA gene and subsequent restriction endonuc...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::Commercially available latex agglutination and coagglutination reagents were evaluated for their ability to detect bacterial antigens in the sera of 165 patients to determine their suitability for rapid diagnosis of pneumonia. These reagents were used to detect the polysaccharide capsular antigens of Haemophilus influ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.25.2.248-250.1987
更新日期:1987-02-01 00:00:00
abstract::Bacterial contamination frequently interferes with successful recovery of the Lyme spirochete from cultures of tissue from Borrelia burgdorferi-infected humans, rodents, or ticks. We used 0.20- and 0.45-microns-pore-size syringe-tip filters to recover spirochetes from cultures contaminated with other bacteria. Low con...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.31.7.1896-1898.1993
更新日期:1993-07-01 00:00:00
abstract::A rapid and highly sensitive real-time PCR detection and quantification method for infectious hypodermal and hematopoietic necrosis virus (IHHNV), a single-stranded DNA virus, and white spot virus (WSV), a double-stranded DNA (dsDNA) virus infecting penaeid shrimp (Penaeus sp.), was developed using the GeneAmp 5700 se...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.39.8.2835-2845.2001
更新日期:2001-08-01 00:00:00
abstract::In October 2001, the first disseminated biological warfare attack was perpetrated on American soil. Initially, a few clinical microbiology laboratories were testing specimens from acutely ill patients and also being asked to test nasal swabs from the potentially exposed. Soon after, a significant number of clinical mi...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章,评审
doi:10.1128/JCM.00359-14
更新日期:2014-07-01 00:00:00
abstract::In total, 521 nonrespiratory specimens (91 urine, 30 gastric aspirate, 245 tissue, 113 pleural fluid, 19 cerebrospinal fluid [CSF], and 23 stool specimens) submitted to the German National Reference Laboratory for Mycobacteria (NRL) from May 2009 to August 2010 were comparatively investigated with the new molecular-ba...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.02268-10
更新日期:2011-04-01 00:00:00
abstract::Hafnia alvei strains which possess the attachment-effacement gene (eaeA) may have clinical importance as new diarrhea-causing pathogens and should therefore be differentiated from other H. alvei strains. We characterized diarrheal H. alvei strains, which were positive in the PCR test for the eaeA gene, using biochemic...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.33.9.2372-2376.1995
更新日期:1995-09-01 00:00:00
abstract::Diagnosis of invasive aspergillosis (IA) requires increasingly rapid molecular methods that enable sensitive detection and discrimination between species. We designed and evaluated a real-time PCR-based method that combined melting temperature (T(m)) calling analysis of a specific probe with high-resolution melting an...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00176-12
更新日期:2012-10-01 00:00:00
abstract::Sensitivities of DNA extraction methods and PCR methods for Giardia duodenalis were evaluated. A combination of the most sensitive methods, i.e., FTA filter paper and a PCR protocol using RH11/RH4 and GiarF/GiarR primers, showed no significant differences compared to immunofluorescence assay in terms of their sensitiv...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01823-06
更新日期:2007-02-01 00:00:00
abstract::A normalized enzyme-linked immunosorbent assay for the determination of immunoglobulin G antibodies to cytomegalovirus is described. The rapid assay involves three 30-min incubations and permits the quantitation of antibody levels with a single-specimen dilution in conjunction with a reference antibody preparation. Th...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.18.1.33-39.1983
更新日期:1983-07-01 00:00:00
abstract::Papillomatous digital dermatitis (PDD) is an infectious foot disease of cattle that is prevalent throughout the world. Although it has been prevalent in Japan since the first case was reported in 1992, full epidemiological and bacteriological examinations have not been conducted. We collected 91 lesions of PDD from 80...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01574-08
更新日期:2009-03-01 00:00:00
abstract::A novel PCR primer pair was used to detect the presence of cmeC in 131 Campylobacter jejuni and C. coli strains isolated from various hosts (cattle, turkeys, humans, and pigs). DNA sequence analysis revealed a high degree of genetic variation between the two species, while extremely limited genetic variation among iso...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01208-07
更新日期:2007-10-01 00:00:00
abstract::Staphylococcal cassette chromosome mec (SCCmec) types and Panton-Valentine leukocidin (PVL) gene carriage were compared among suspected community-associated methicillin-resistant Staphylococcus aureus MRSA (CA-MRSA) and health care-associated MRSA (HA-MRSA) isolates. CA-MRSA isolates carried the SCCmec type IV complex...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01706-06
更新日期:2007-03-01 00:00:00
abstract::Capnocytophaga ochracea is a gram-negative, fusiform bacillus which is part of the normal human oral flora. This organism is often isolated from periodontal lesions of patients with periodontitis and is associated with sepsis in granulocytopenic patients. We report here a case of endocarditis caused by C. ochracea. ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.26.5.1061-1062.1988
更新日期:1988-05-01 00:00:00
abstract::Spontaneously occurring urinary tract infection (UTI) in dogs was exploited as an experiment of nature to gain insights into UTI pathogenesis in humans. Concurrent urinary and rectal Escherichia coli isolates from 37 dogs with UTI were compared with respect to phylogenetic background, O antigens, and extended virulenc...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/jcm.41.1.337-345.2003
更新日期:2003-01-01 00:00:00