Abstract:
:To compare Simplexa Flu A/B & RSV PCR with cytospin-immunofluorescence and laboratory-developed TaqMan PCR methods, 445 nasopharyngeal samples were tested. Of these, 199 were positive (46 for respiratory syncytial virus [RSV], 120 for influenza A, and 33 for influenza B) and 246 were negative. The direct fluorescent-antibody assay (DFA) detected 132 (66.3%) positive samples, Simplexa direct detected 162 (81.4%), Simplexa using extracts detected 177 (88.9%), and lab-developed TaqMan PCR reference methods detected 199 (100%). The specificities were 99.6%, 100%, 100%, and 100%, respectively. The two Simplexa methods were more sensitive than the DFA (P = 0.0001) but less sensitive than the TaqMan reverse transcriptase PCR (P = 0.0001).
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Landry ML,Ferguson Ddoi
10.1128/JCM.00738-14subject
Has Abstractpub_date
2014-08-01 00:00:00pages
3057-9issue
8eissn
0095-1137issn
1098-660Xpii
JCM.00738-14journal_volume
52pub_type
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journal_title:Journal of clinical microbiology
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