Rapid Identification of Measles Virus Vaccine Genotype by Real-Time PCR.

abstract：:There is a lack of consensus among investigators who use a variety of immunoassay techniques (e.g., enzyme-linked immunosorbent assay [ELISA] and radioimmunoassay) regarding the protocols for describing and forming standard reference or calibration curves and interpolating serum antibody concentrations. This confounds...

journal_title：Journal of clinical microbiology

authors： Plikaytis BD,Holder PF,Pais LB,Maslanka SE,Gheesling LL,Carlone GM

更新日期：1994-10-01 00:00:00

abstract：:A new test principle for the simultaneous detection of total approximate titers and immunoglobulin M antibodies has been developed and applied to the detection of antibody to hepatitis B core antigen. The method is based on the combination of a competition radioimmunoassay, for the determination of total antibody tite...

journal_title：Journal of clinical microbiology

authors： Angarano G,Monno L,Santantonio TA,Pastore G

更新日期：1984-06-01 00:00:00

abstract：:A rapid method for detection of respiratory syncytial virus (RSV) in nasopharyngeal aspirates, involving a combination of reverse transcription and polymerase chain reaction amplification (RT-PCR), has been developed. The RT-PCR assay employs oligonucleotide primers specific for the region of the RSV genome which enco...

journal_title：Journal of clinical microbiology

authors： Paton AW,Paton JC,Lawrence AJ,Goldwater PN,Harris RJ

更新日期：1992-04-01 00:00:00

abstract：:Interferon gamma (IFN-γ) release assays (IGRAs) are increasingly used to test for latent tuberculosis (TB) infection. Although highly specific, IGRAs have a relatively high false-negative rate in active TB patients. A more sensitive assay is needed. IFN-γ-induced protein 10 (IP-10) is an alternative biomarker with a 1...

journal_title：Journal of clinical microbiology

authors： Blauenfeldt T,Villar-Hernández R,García-García E,Latorre I,Holm LL,Muriel-Moreno B,De Souza-Galvão ML,Millet JP,Sabriá F,Sánchez-Montalva A,Ruiz-Manzano J,Pilarte J,Jiménez MA,Centeno C,Torres C,Molina-Pinargote I,Gonzále

更新日期：2020-09-22 00:00:00

abstract：:A group of 300 clinically derived isolates of coagulase-negative staphylococci were tested in parallel with the API STAPH-IDENT system (Analytab Products) and 14 conventional biochemical tests contained in Kloos and Schleifer's simplified scheme for identification of human Staphylococcus species. STAPH-IDENT is a mini...

journal_title：Journal of clinical microbiology

authors： Almeida RJ,Jorgensen JH

更新日期：1983-08-01 00:00:00

abstract：:While Corynebacterium ulcerans can mimic classical diphtheria, extrapharyngeal infections are extremely rare. Sequencing of the diphtheria toxin (DT)-encoding tox gene of two C. ulcerans isolates from extrapharyngeal infections revealed differences from C. diphtheriae DT sequences, mainly in the translocation and rece...

journal_title：Journal of clinical microbiology

authors： Sing A,Hogardt M,Bierschenk S,Heesemann J

更新日期：2003-10-01 00:00:00

abstract：:Test strips for the detection of antibodies to human immunodeficiency virus type 1 were investigated using specimens from risk groups in Thailand (141 reactive; 445 nonreactive) in a local Thai laboratory. The diagnostic sensitivity and specificity were both 100%. Using a set of seroconversion panels, the sensitivity ...

journal_title：Journal of clinical microbiology

authors： Pagcharoenpol P,Burgess-Cassler A,Schramm W

更新日期：1996-04-01 00:00:00

abstract：:A comparative study of herpes simplex virus diagnosis by standard cell culture and a new hybrid test (enzyme-linked immunosorbent assay spin amplification technique) was done on 300 specimens. The new test was found to be equally sensitive and specific, much less expensive to perform, and to report all results in 48 h...

journal_title：Journal of clinical microbiology

authors： Michalski FJ,Shaikh M,Sahraie F,Desai S,Verano L,Vallabhaneni J

更新日期：1986-08-01 00:00:00

abstract：:The volume of blood sampled for blood culture determines its sensitivity. We measured low mean blood volumes in submitted aerobic (8.38 ± 3.88 ml) and anaerobic (7.16 ± 3.83 ml) blood culture bottles. Educational seminars were held for phlebotomy teams, and renewed measurements thereafter revealed significantly higher...

journal_title：Journal of clinical microbiology

authors： van Ingen J,Hilt N,Bosboom R

更新日期：2013-03-01 00:00:00

abstract：:The QuadFERM+ system (Analytab Products, Plainview, N.Y.), a 2-h carbohydrate degradation method for the identification of Neisseria spp., was evaluated along with a rapid DNase test for confirmation of Branhamella catarrhalis. QuadFERM+ identified 100% of 82 N. gonorrhoeae and 96% of 54 N. meningitidis strains. The t...

journal_title：Journal of clinical microbiology

authors： Janda WM,Zigler KL,Bradna JJ

更新日期：1987-02-01 00:00:00

abstract：:Parameters like zone reading, inoculum density, and plate streaking influence the precision and accuracy of disk diffusion antibiotic susceptibility testing (AST). While improved reading precision has been demonstrated using automated imaging systems, standardization of the inoculum and of plate streaking have not bee...

journal_title：Journal of clinical microbiology

authors： Hombach M,Maurer FP,Pfiffner T,Böttger EC,Furrer R

更新日期：2015-12-01 00:00:00

abstract：:Investigations of nursery outbreaks of Citrobacter diversus sepsis and meningitis have been hampered by lack of adequate epidemiologic markers for the organism. We studied outer membrane protein profiles from clinical isolates of C. diversus by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to determine whe...

journal_title：Journal of clinical microbiology

authors： Kline MW,Mason EO Jr,Kaplan SL

更新日期：1989-08-01 00:00:00

abstract：:Reference strains of serotypes 1 to 12 of Actinobacillus (Haemophilus) pleuropneumoniae were cultured in Eagle minimal essential medium with 10% Serum Plus. Culture supernatants were examined for cytotoxicity to alveolar macrophages and for the ability to hemolyze sheep erythrocytes. All strains except the reference s...

journal_title：Journal of clinical microbiology

authors： Kamp EM,van Leengoed LA

更新日期：1989-06-01 00:00:00

abstract：:A directory of test patterns and their interpretations has been prepared for identification of Enterobacteriaceae by using the 11-test API 10 S kit. The diagnostic accuracy of the directory and kit were evaluated by using records of test results for 37,476 isolates studied with the 21-test API 20 Enteric kit. Analysis...

journal_title：Journal of clinical microbiology

authors： Robertson EA,MacLowry JD

更新日期：1975-06-01 00:00:00

abstract：:Nontuberculous mycobacteria are ubiquitous in the environment but rarely infect immunocompetent patients. We describe a pediatric case of Mycobacterium simiae complex lymphadenitis in an immunocompetent child and review the natural history, clinical manifestations, diagnosis, and current management of the disease. ...

journal_title：Journal of clinical microbiology

authors： Cruz AT,Goytia VK,Starke JR

更新日期：2007-08-01 00:00:00

abstract：:Real-time PCR analysis of the total bacterial load in advanced carious lesions has shown that the total load exceeds the number of cultivable bacteria. This suggests that an unresolved complexity exists in bacteria associated with advanced caries. In this report, the profile of the microflora of carious dentine was ex...

journal_title：Journal of clinical microbiology

authors： Chhour KL,Nadkarni MA,Byun R,Martin FE,Jacques NA,Hunter N

更新日期：2005-02-01 00:00:00

abstract：:Two multiplex PCRs were developed for the detection of necrotoxigenic Escherichia coli virulence genes. M1 contained the primers for the toxins and the aerobactin, and M2 contained the primers for the adhesins. They were validated by single PCRs performed with reference E. coli strains and by multiplex PCRs with necro...

journal_title：Journal of clinical microbiology

authors： Van Bost S,Jacquemin E,Oswald E,Mainil J

更新日期：2003-09-01 00:00:00

abstract：:We report the first case of a postoperative wound infection caused by Vibrio metschnikovii on the lower right leg of a patient after saphenectomy. Compared to the healing of an uninfected site, that of the right leg was delayed, and a cure was achieved by intensified wound care. Several swabs taken from the infected s...

journal_title：Journal of clinical microbiology

authors： Linde HJ,Kobuch R,Jayasinghe S,Reischl U,Lehn N,Kaulfuss S,Beutin L

更新日期：2004-10-01 00:00:00

abstract：:Human adenovirus of strains subgenus F (AdV F) are the most common strains detected in acute gastroenteritis cases in developing countries. Subgenus F is represented by AdV serotype 40 (AdV-40) and AdV-41. Most of the reports have described the predominance of AdV-41 in acute gastroenteritis cases. To gain insight int...

journal_title：Journal of clinical microbiology

authors： Dey RS,Ghosh S,Chawla-Sarkar M,Panchalingam S,Nataro JP,Sur D,Manna B,Ramamurthy T

更新日期：2011-02-01 00:00:00

abstract：:Yarrowia lipolytica is a weakly pathogenic yeast that is rarely isolated from the blood. We observed transient recurrent catheter-related fungemia attributable to this organism in a leukemic patient. The fungemia and accompanying fever subsided spontaneously. The data suggest that it might be possible to withhold spec...

journal_title：Journal of clinical microbiology

authors： Chang CL,Park TH,Lee EY,Lim YT,Son HC

更新日期：2001-03-01 00:00:00

abstract：:Laboratory tests are essential for confirming sporadic cases and outbreaks of rubella. Detection of rubella virus is often necessary to confirm rubella cases and to identify specimens to be used to characterize wild-type rubella viruses. The sensitivities of four methods for detecting rubella virus infection using thr...

journal_title：Journal of clinical microbiology

authors： Zhu Z,Xu W,Abernathy ES,Chen MH,Zheng Q,Wang T,Zhang Z,Li C,Wang C,He W,Zhou S,Icenogle J

更新日期：2007-09-01 00:00:00

abstract：:Septic arthritis of the knee developed in a 21-month-old child. The causative organism, isolated from two separate arthrocenteses, was identified as Capnocytophaga ochracea morphologically and by biochemical reactions. Previous human infections (bacteremias) have occurred in granulocytopenic hosts with concomitant ora...

journal_title：Journal of clinical microbiology

authors： Winn RE,Chase WF,Lauderdale PW,McCleskey FK

更新日期：1984-04-01 00:00:00

abstract：:Clostridium perfringens is an important pathogen of animals and humans and is the causative agent of necrotic enteritis (NE) in poultry. This study focuses on the typing of intestinal C. perfringens isolates (n = 61) from outbreaks of NE collected from several areas of Southern Ontario, using a recently developed mult...

journal_title：Journal of clinical microbiology

authors： Chalmers G,Bruce HL,Hunter DB,Parreira VR,Kulkarni RR,Jiang YF,Prescott JF,Boerlin P

更新日期：2008-12-01 00:00:00

abstract：:A premarket trial of the Becton Dickinson Directigen respiratory syncytial virus membrane-based enzyme immunoassay compared the test with virus isolation for the detection of respiratory syncytial virus in 583 nasopharyngeal aspirates. After modification, the Directigen test showed a sensitivity of 83% and a specifici...

journal_title：Journal of clinical microbiology

authors： Kok T,Barancek K,Burrell CJ

更新日期：1990-06-01 00:00:00

abstract：:We investigated the performance of a double-antigen sandwich recombinant enzyme immunoassay (EIA; Abbott Laboratories, North Chicago, Ill.) and compared it with that of a synthetic-peptide-based EIA (Biochem Immunosystems, Montreal, Quebec, Canada) for the detection of human immunodeficiency type 1 (HIV-1) and HIV-2 a...

journal_title：Journal of clinical microbiology

authors： Galli RA,Castriciano S,Fearon M,Major C,Choi KW,Mahony J,Chernesky M

更新日期：1996-04-01 00:00:00

abstract：:The objective of this study was to evaluate the clinical characteristics and outcomes of hospitalized patients tested for Clostridium difficile and determine the correlation between pretest probability for C. difficile infection (CDI) and assay results. Patients with testing ordered for C. difficile were enrolled and ...

journal_title：Journal of clinical microbiology

authors： Kwon JH,Reske KA,Hink T,Burnham CA,Dubberke ER

更新日期：2017-02-01 00:00:00

abstract：:We compared culture results to investigate the influence of antimicrobial-loaded cement on sonicate fluid culture positivity for the diagnosis of prosthetic joint infection. Fifty-four subjects were assessed. The sensitivities of sonicate fluid culture were 77.8% (14 of 18) in subjects with an antimicrobial-loaded cem...

journal_title：Journal of clinical microbiology

authors： Park KH,Greenwood-Quaintance KE,Hanssen AD,Abdel MP,Patel R

更新日期：2016-06-01 00:00:00

abstract：:Streptococcus pneumoniae is a major cause of bacteremia, meningitis, pneumonia, sinusitis, and acute otitis media in children. Although optochin susceptibility, bile solubility, and Quellung testing are the standards for identifying and differentiating pneumococci, there are several reports of nontypeable pneumococci ...

journal_title：Journal of clinical microbiology

authors： Ing J,Mason EO,Kaplan SL,Lamberth LB,Revell PA,Luna RA,Hulten KG

更新日期：2012-04-01 00:00:00

abstract：:Enteroaggregative Escherichia coli (EAEC) is an emerging enteric pathogen that causes acute and chronic diarrhea among children, human immunodeficiency virus-infected patients, and travelers to developing regions of the world. The pathogenesis of EAEC strains involves the production of biofilm. In this study, we deter...

journal_title：Journal of clinical microbiology

authors： Mohamed JA,Huang DB,Jiang ZD,DuPont HL,Nataro JP,Belkind-Gerson J,Okhuysen PC

更新日期：2007-01-01 00:00:00

abstract：:Members of the genus Malassezia, lipophilic yeasts, are considered to be one of the exacerbating factors in atopic dermatitis (AD). We examined variation in cutaneous colonization by Malassezia species in AD patients and compared it with variation in healthy subjects. Samples were collected by applying transparent dre...

journal_title：Journal of clinical microbiology

authors： Sugita T,Suto H,Unno T,Tsuboi R,Ogawa H,Shinoda T,Nishikawa A

更新日期：2001-10-01 00:00:00