Absence of serotype-specific surface antigen and altered teichoic acid glycosylation among epidemic-associated strains of Listeria monocytogenes.

abstract：:Survival of anaerobic and aerobic bacteria in a commercially available, non-supportive, gassed (oxygen-free) transport container (Anaport) was evaluated quantitatively. Saline-suspended obligate anaerobes survived significantly better in the gassed container in aerobic control tubes (P less than 0.025, t test), and co...

journal_title：Journal of clinical microbiology

authors： Chow AW,Cunningham PJ,Guze LB

更新日期：1976-02-01 00:00:00

abstract：:PCR-single-strand conformation polymorphism (PCR-SSCP) analysis is a rapid and convenient technique for the detection of mutations and allelic variants. We have adapted this technique for the identification of bacteria by PCR with fluorescein-labeled primers chosen from the conserved regions of the 16S rRNA gene flank...

journal_title：Journal of clinical microbiology

authors： Widjojoatmodjo MN,Fluit AC,Verhoef J

更新日期：1995-10-01 00:00:00

abstract：:A TaqMan PCR was developed for quantifying early colonizer microorganisms in dental biofilms. To design species-specific primers and TaqMan probes, genomic subtractive hybridization was used. This quantitative assay in combination with subtractive hybridization may be of value in the study of microbial ecosystems cons...

journal_title：Journal of clinical microbiology

authors： Suzuki N,Nakano Y,Yoshida A,Yamashita Y,Kiyoura Y

更新日期：2004-08-01 00:00:00

abstract：:Feces and serum specimens were collected from three farms in Michigan on which approximately 50-lb (8- to 9-week-old) pigs experienced diarrhea just after placement into all-in-all-out finishing barns. The clinical signs (profuse watery diarrhea lasting about 2 weeks and no vomiting) were similar on all farms, and the...

journal_title：Journal of clinical microbiology

authors： Kim Y,Chang KO,Straw B,Saif LJ

更新日期：1999-05-01 00:00:00

abstract：:Many commercially available media for cultivation of mycobacteria have failed to support the growth of these organisms. This is especially true of media prepared with albumin-containing enrichments. Earlier, we developed a method for rapid identification of good albumin enrichments for agar-based media used to test th...

journal_title：Journal of clinical microbiology

authors： Butler WR,Warren NG,Kubica GP,Kilburn JO

更新日期：1990-05-01 00:00:00

abstract：:Human papillomavirus (HPV) plays a key role in the development of cervical and laryngeal cancers. The aim of our study was to compare the performance of a new hydrogel-based HPV genotyping biochip assay (Biochip) to a commercially available and CE-marked conventional PCR followed by reverse hybridization (GenID-PCR). ...

journal_title：Journal of clinical microbiology

authors： Schenk T,Brandstetter T,Zur Hausen A,Alt-Mörbe J,Huzly D,Rühe J

更新日期：2009-05-01 00:00:00

abstract：:A 24-year-old man sustained a crush injury to the tibia, which subsequently became infected with Saksenaea vasiformis. He was treated with debridement and a free myocutaneous flap, but amputation was necessary because of mycotic osteomyelitis. S. vasiformis was recovered and identified on the basis of its characterist...

journal_title：Journal of clinical microbiology

authors： Pierce PF,Wood MB,Roberts GD,Fitzgerald RH Jr,Robertson C,Edson RS

更新日期：1987-05-01 00:00:00

abstract：:Nosocomial bronchopneumonia is a frequent complication in patients with chronic intratracheal intubation. Despite targeted antibiotic treatment, production of abundant bronchial secretion containing pathogen bacteria often tends to be chronic, and so mortality drastically increases. This problem led to an investigatio...

journal_title：Journal of clinical microbiology

authors： Klekner A,Bagyi K,Bognar L,Gaspar A,Andrasi M,Szabo J

更新日期：2006-09-01 00:00:00

abstract：:The NucliSENS easyMAG automated system was compared to the column-based Qiagen method for Epstein-Barr virus (EBV) or cytomegalovirus (CMV) DNA extraction from whole blood before viral load determination using the corresponding R-gene amplification kits. Both extraction techniques exhibited a total agreement of 81.3% ...

journal_title：Journal of clinical microbiology

authors： Pillet S,Bourlet T,Pozzetto B

更新日期：2009-11-01 00:00:00

abstract：:Between September 1995 and August 1998, the incidence and diversity of the main human rotavirus genotypes (G1, G2, G3, and G4 and P[8], P[4], P[6], and P[9]) among Irish children were determined by using established and adapted reverse transcriptase PCR-based genotyping methods. From a total of 193 rotavirus-positive ...

journal_title：Journal of clinical microbiology

authors： O'Mahony J,Foley B,Morgan S,Morgan JG,Hill C

更新日期：1999-06-01 00:00:00

abstract：:Inactivation of penicillin and gentamicin in cultures was achieved by using monoclonal antibodies against these antibiotics. A viridans group streptococcus (penicillin MIC, less than or equal to 0.06 micrograms/ml) and Escherichia coli ATCC 35218 (gentamicin MIC, less than or equal to 1 microgram/ml) were able to grow...

journal_title：Journal of clinical microbiology

authors： Sierra-Madero JG,Caulfield MJ,Hall GS,Washington JA

更新日期：1988-09-01 00:00:00

abstract：:In order to compare methods for subtyping Neisseria meningitidis serogroup B isolates, 96 isolates obtained from various locations in the United States and northwestern Europe were subtyped by five methods: monoclonal antibody (MAb)-based serotyping and serosubtyping, DNA macrorestriction analysis by pulsed-field gel ...

journal_title：Journal of clinical microbiology

authors： Swaminathan B,Matar GM,Reeves MW,Graves LM,Ajello G,Bibb WF,Helsel LO,Morales M,Dronavalli H,el-Swify M,DeWitt W,Hunter SB

更新日期：1996-06-01 00:00:00

abstract：:Molecular typing has become indispensable in the detection of nosocomial transmission of bacterial pathogens and the identification of sources and routes of transmission in outbreak settings, but current methods are labor-intensive, are difficult to standardize, or have limited resolution. Whole-genome multilocus sequ...

journal_title：Journal of clinical microbiology

authors： Kluytmans-van den Bergh MF,Rossen JW,Bruijning-Verhagen PC,Bonten MJ,Friedrich AW,Vandenbroucke-Grauls CM,Willems RJ,Kluytmans JA

更新日期：2016-12-01 00:00:00

abstract：:Nucleic acid tests are sensitive and specific and provide a rapid diagnosis, making them invaluable for patient and outbreak management. Multiplex PCR assays have additional advantages in providing an economical and comprehensive panel for many common respiratory viruses. Previous reports have shown the utility of the...

journal_title：Journal of clinical microbiology

authors： Pabbaraju K,Wong S,Tokaryk KL,Fonseca K,Drews SJ

更新日期：2011-05-01 00:00:00

abstract：:Although numerous perioperative samples and culture media are required to diagnose prosthetic joint infection (PJI), their exact number and types have not yet been definitely determined with a high level of proof. We conducted a prospective multicenter study to determine the minimal number of samples and culture media...

journal_title：Journal of clinical microbiology

authors： Bémer P,Léger J,Tandé D,Plouzeau C,Valentin AS,Jolivet-Gougeon A,Lemarié C,Kempf M,Héry-Arnaud G,Bret L,Juvin ME,Giraudeau B,Corvec S,Burucoa C,Centre de Référence des Infections Ostéo-articulaires du Grand Ouest (CRIOGO) Stu

更新日期：2016-02-01 00:00:00

abstract：:The reliability of an rRNA-derived oligonucleotide probe for Neisseria gonorrhoeae was tested with 187 N. gonorrhoeae isolates, 81 Neisseria meningitidis isolates, and several strains of other bacterial species. The probe proved to be 100% specific and 100% sensitive. N. gonorrhoeae cells could also be reliably identi...

journal_title：Journal of clinical microbiology

authors： Rossau R,Duhamel M,Van Dyck E,Piot P,Van Heuverswyn H

更新日期：1990-05-01 00:00:00

abstract：:Restriction endonucleases are bacterial enzymes that cleave DNA at specific sites. The resulting DNA fragments may be separated electrophoretically in gel to form specific restriction patterns. In the present study, the restriction endonuclease method was successfully adapted to the analysis of the chromosomal DNA of ...

journal_title：Journal of clinical microbiology

authors： Bjorvatn B,Lund V,Kristiansen BE,Korsnes L,Spanne O,Lindqvist B

更新日期：1984-06-01 00:00:00

abstract：:The polymerase chain reaction was used to detect human herpesvirus 6 (HHV-6) DNA in peripheral blood mononuclear cells from patients with exanthem subitum. Amplified products were detected by agarose gel electrophoresis and dot blot hybridization with a cloned DNA probe. No cross-hybridization with DNAs of five other ...

journal_title：Journal of clinical microbiology

authors： Kondo K,Hayakawa Y,Mori H,Sato S,Kondo T,Takahashi K,Minamishima Y,Takahashi M,Yamanishi K

更新日期：1990-05-01 00:00:00

abstract：:Prostate tissues from patients with prostate cancer and benign prostatic hyperplasia (BPH) frequently contain histological inflammation, and a proportion of these patients show evidence of Propionibacterium acnes infection in the prostate gland. We developed a multicolor fluorescent in situ hybridization (FISH) assay ...

journal_title：Journal of clinical microbiology

authors： Alexeyev OA,Marklund I,Shannon B,Golovleva I,Olsson J,Andersson C,Eriksson I,Cohen R,Elgh F

更新日期：2007-11-01 00:00:00

abstract：:By quantitative gas-liquid chromatography of glucose-containing blood cultures at the moment of first signs of growth, a presumptive diagnosis of anaerobic bacteremia could be made in 24 out of 26 cultures yielding obligate anaerobes upon subsequent culture. With Bacteroides sp. (20 strains isolated), elevated levels ...

journal_title：Journal of clinical microbiology

authors： Wüst J

更新日期：1977-12-01 00:00:00

abstract：:Rotaviruses contain a double-stranded ribonucleic acid genome consisting of 11 segments. Gel electrophoresis separates genome segments and allows identification of strain differences. This electrophoretic typing technique was applied to rotavirus specimens from 116 children and 72 newborn babies. Between 1973 and 1979...

journal_title：Journal of clinical microbiology

authors： Rodger SM,Bishop RF,Birch C,McLean B,Holmes IH

更新日期：1981-02-01 00:00:00

abstract：:Capnocytophaga ochracea is a gram-negative, fusiform bacillus which is part of the normal human oral flora. This organism is often isolated from periodontal lesions of patients with periodontitis and is associated with sepsis in granulocytopenic patients. We report here a case of endocarditis caused by C. ochracea. ...

journal_title：Journal of clinical microbiology

authors： Buu-Hoi AY,Joundy S,Acar JF

更新日期：1988-05-01 00:00:00

abstract：:Brucellosis in Argentina is currently investigated in bank donor blood by the standard plate agglutination test (PAT). This study evaluated the buffered plate antigen test (BPA), now used to screen for bovine brucellosis, as a screen for human disease. Of 57 sera from patients with culture-confirmed brucellosis, 100% ...

journal_title：Journal of clinical microbiology

authors： Lucero NE,Bolpe JE

更新日期：1998-05-01 00:00:00

abstract：:We evaluated quantitative real-time PCR to establish the diagnosis of rotavirus gastroenteritis in a high-disease-burden population in Malawi using enzyme immunoassay as the gold standard diagnostic test. In 146 children with acute gastroenteritis and 65 asymptomatic children, we defined a cutoff point in the threshol...

journal_title：Journal of clinical microbiology

authors： Bennett A,Bar-Zeev N,Jere KC,Tate JE,Parashar UD,Nakagomi O,Heyderman RS,French N,Iturriza-Gomara M,Cunliffe NA

更新日期：2015-06-01 00:00:00

abstract：:A protocol was optimized for the isolation of Mycobacterium avium subsp. paratuberculosis (MAP) from milk and colostrum, with parameters including chemical decontamination, antibiotics, and different culture media. This study demonstrates that the efficiency of MAP recovery from milk is highly dependent upon the cultu...

journal_title：Journal of clinical microbiology

authors： Bradner L,Robbe-Austerman S,Beitz DC,Stabel JR

更新日期：2013-05-01 00:00:00

abstract：:We determined the entire nucleotide sequence of phiSa2958-carrying Panton-Valentine leukocidin (PVL) gene, which was lysogenized in a sequence type 5 staphylococcal cassette chromosome mec (SCCmec) type II strain of methicillin-resistant Staphylococcus aureus (MRSA). Based on the nucleotide sequences of PVL phages, we...

journal_title：Journal of clinical microbiology

authors： Ma XX,Ito T,Kondo Y,Cho M,Yoshizawa Y,Kaneko J,Katai A,Higashiide M,Li S,Hiramatsu K

更新日期：2008-10-01 00:00:00

abstract：:An Escherichia coli rRNA probe was compared with a combination of O serotyping, phage susceptibility, and biotype pattern for the type identification of strains of Enterobacter cloacae. Forty-five isolates of E. cloacae from 36 patients in nine hospitals were examined. By conventional typing, only 26 (57.7%) could be ...

journal_title：Journal of clinical microbiology

authors： Garaizar J,Kaufmann ME,Pitt TL

更新日期：1991-07-01 00:00:00

abstract：:The purpose of the investigation was to evaluate two commercially available identification systems: a new modification of the Staph-Zym system (Rosco, Tåstrup, Denmark) and the Staph ID 32 API system (API System, BioMérieux, Paris, France). A local standard method to be used in routine laboratories was also evaluated....

journal_title：Journal of clinical microbiology

authors： Renneberg J,Rieneck K,Gutschik E

更新日期：1995-05-01 00:00:00

abstract：:Invasive aspergillosis (IA) is a life-threatening infection that mainly occurs in immunocompromised patients. Here, we compared the novel Aspergillus-specific galactomannoprotein (GP) enzyme-linked immunosorbent assay (ELISA) (Euroimmun Medizinische Labordiagnostika AG) to the established Platelia Aspergillus galactom...

journal_title：Journal of clinical microbiology

authors： Dichtl K,Seybold U,Ormanns S,Horns H,Wagener J

更新日期：2019-06-25 00:00:00

abstract：:A group of 254 isolates of coagulase-negative staphylococci were tested in parallel for novobiocin susceptibility by using P agar and Mueller-Hinton agar. Zones of inhibition of 16 mm or less around a 5-micrograms novobiocin disk on Mueller-Hinton agar indicated novobiocin resistance, as demonstrated by Staphylococcus...

journal_title：Journal of clinical microbiology

authors： Almeida RJ,Jorgensen JH

更新日期：1982-12-01 00:00:00