Buffered plate antigen test as a screening test for diagnosis of human brucellosis.

abstract：:Levels of amikacin in serum were determined in 106 serum specimens by a latex agglutination inhibition card test and by radioimmunoassay (RIA). Linear regression analysis demonstrated a high degree of correlation between the two assays (latex = 0.95 (RIA) + 0.69; r = 0.97). Assay of three control sera containing 7.5, ...

journal_title：Journal of clinical microbiology

authors： Khabbaz RF,Standiford HC,Bernstein D,Nipper HC,Tatem BA,Smalls U,Drusano GL,Caplan E

更新日期：1985-11-01 00:00:00

abstract：:Antibodies to human papilloma virus (HPV) type 16 (HPV-16) E6 and E7 proteins in serum are markers for HPV-associated invasive cervical carcinoma. We compared two assays, a radioimmunoprecipitation assay with in vitro-translated HPV-16 E6 and E7 proteins and an enzyme-linked immunosorbent assay (ELISA) with E6 and E7 ...

journal_title：Journal of clinical microbiology

authors： Sun Y,Shah KV,Müller M,Muñoz N,Bosch XF,Viscidi RP

更新日期：1994-09-01 00:00:00

abstract：:Forty-eight clinical Acinetobacter isolates with different epidemic behavior were investigated for the presence of integrons and plasmids and for antibiotic susceptibility. Integrons were demonstrated in 50% of the strains by an integrase gene PCR. Epidemic strains of Acinetobacter baumannii were found to contain sign...

journal_title：Journal of clinical microbiology

authors： Koeleman JG,Stoof J,Van Der Bijl MW,Vandenbroucke-Grauls CM,Savelkoul PH

更新日期：2001-01-01 00:00:00

abstract：:In order to assess the microbiologic and clinical value of primary broth culture of wound specimens collected with swabs and submitted to the laboratory in transport medium, we compared the results of primary agar culture with the results of a corresponding primary broth culture for 344 aerobic specimens and 176 anaer...

journal_title：Journal of clinical microbiology

authors： Silletti RP,Ailey E,Sun S,Tang D

更新日期：1997-08-01 00:00:00

abstract：:Ehrlichia canis causes a potentially fatal rickettsial disease of dogs that requires rapid and accurate diagnosis in order to initiate appropriate therapy leading to a favorable prognosis. We recently reported the cloning of two immunoreactive E. canis proteins, P28 and P140, that were applicable for serodiagnosis of ...

journal_title：Journal of clinical microbiology

authors： McBride JW,Corstvet RE,Breitschwerdt EB,Walker DH

更新日期：2001-01-01 00:00:00

abstract：:An aerobically incubated, agar-based medium was developed for amino acid decarboxylase testing of Enterobacteriaceae family members by replica-plating methods. Results with the new medium agreed 97 to 99% with the reference broth method of Moeller, and no false-positive reactions were encountered. ...

journal_title：Journal of clinical microbiology

authors： Maccani JE

更新日期：1979-12-01 00:00:00

abstract：:A previously established multiplex PCR that identifies to the species level Acinetobacter baumannii and Acinetobacter genomic species 13TU (GS13TU) was expanded to include Acinetobacter calcoaceticus and Acinetobacter genomic species 3. ...

journal_title：Journal of clinical microbiology

authors： Higgins PG,Lehmann M,Wisplinghoff H,Seifert H

更新日期：2010-12-01 00:00:00

abstract：:An agglutination test for immunoglobulin G (IgG) Toxoplasma antibodies with acetone-fixed tachyzoites (AC antigens; AC agglutination test) was positive only with sera from patients during the acute stage of their infection. In contrast, when the test was performed with Formalin-fixed tachyzoites (HS antigens; HS agglu...

journal_title：Journal of clinical microbiology

authors： Suzuki Y,Thulliez P,Desmonts G,Remington JS

更新日期：1988-05-01 00:00:00

abstract：:Ceftolozane-tazobactam (C/T) is a novel beta-lactam-beta-lactamase inhibitor combination antibiotic approved by the U.S. Food and Drug Administration in 2014 for the treatment of complicated intra-abdominal infections (in combination with metronidazole) and complicated urinary tract infections. In this study, we evalu...

journal_title：Journal of clinical microbiology

authors： Bailey AL,Armstrong T,Dwivedi HP,Denys GA,Hindler J,Campeau S,Traczewski M,Humphries R,Burnham CA

更新日期：2018-08-27 00:00:00

abstract：:A Congo red-magnesium oxalate agar medium was developed to detect expression of virulence-associated calcium dependency and Congo red absorption in Yersinia enterocolitica. Of the 157 pathogenic serotypes tested, 119 (75.8%) were positive; 98% of nonpathogenic serotypes and strains of three other Yersinia species were...

journal_title：Journal of clinical microbiology

authors： Riley G,Toma S

更新日期：1989-01-01 00:00:00

abstract：:A total of 800 colonies suggestive of Salmonella, Shigella, or Yersinia species isolated on stool differential agar media were inoculated onto both conventional biochemical test media (triple sugar iron agar, urea agar, and phenylalanine agar) and Entero Pathogen Screen cards of the AutoMicrobic system (Vitek Systems,...

journal_title：Journal of clinical microbiology

authors： Villasante PA,Agulla A,Merino FJ,Pérez T,Ladrón de Guevara C,Velasco AC

更新日期：1987-03-01 00:00:00

abstract：:Jena virus (JV) is a bovine enteric calicivirus that causes diarrhea in calves. The virus is approximately 30 nm in diameter and has a surface morphology similar to the human Norwalk virus. The genome sequence of JV was recently described, and the virus has been assigned to the genus Norovirus of the family CALICIVIRI...

journal_title：Journal of clinical microbiology

authors： Deng Y,Batten CA,Liu BL,Lambden PR,Elschner M,Günther H,Otto P,Schnürch P,Eichhorn W,Herbst W,Clarke IN

更新日期：2003-06-01 00:00:00

abstract：:Investigations of nursery outbreaks of Citrobacter diversus sepsis and meningitis have been hampered by lack of adequate epidemiologic markers for the organism. We studied outer membrane protein profiles from clinical isolates of C. diversus by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to determine whe...

journal_title：Journal of clinical microbiology

authors： Kline MW,Mason EO Jr,Kaplan SL

更新日期：1989-08-01 00:00:00

abstract：:A rapid and highly sensitive real-time PCR detection and quantification method for infectious hypodermal and hematopoietic necrosis virus (IHHNV), a single-stranded DNA virus, and white spot virus (WSV), a double-stranded DNA (dsDNA) virus infecting penaeid shrimp (Penaeus sp.), was developed using the GeneAmp 5700 se...

journal_title：Journal of clinical microbiology

authors： Dhar AK,Roux MM,Klimpel KR

更新日期：2001-08-01 00:00:00

abstract：:We report an unusual case of culture-proven pneumococcal and meningococcal mixed meningitis in an 18-month-old girl. The patient responded well to antimicrobial therapy and recovered completely without sequelae. No underlying condition could be demonstrated except a rhinitis of unknown etiology 2 days before the onset...

journal_title：Journal of clinical microbiology

authors： Marchandin H,Ventura V,Alonso JM,Van de Perre P

更新日期：2005-03-01 00:00:00

abstract：:Direct heteroduplex analysis and a universal heteroduplex generator assay were performed to detect rifampin resistance rapidly in Turkish Mycobacterium tuberculosis isolates. Cross-resistance to rifapentine, rifabutin, and rifalazil was investigated. A relationship between specific mutations and resistance patterns, w...

journal_title：Journal of clinical microbiology

authors： Saribaş Z,Kocagöz T,Alp A,Günalp A

更新日期：2003-02-01 00:00:00

abstract：:Rapid diagnostic tests (RDTs) based on the detection of specific antibodies in serum are commonly used for the diagnosis of visceral leishmaniasis (VL). Several commercial kits are available, and some of them allow the use of whole-blood samples instead of serum. An RDT is much more user-friendly for blood samples tha...

journal_title：Journal of clinical microbiology

authors： Kumar D,Khanal B,Tiwary P,Mudavath SL,Tiwary NK,Singh R,Koirala K,Boelaert M,Rijal S,Sundar S

更新日期：2013-12-01 00:00:00

abstract：:The usefulness of randomly amplified polymorphic DNA method (RAPD) to identify each species of genus Erysipelothrix and for epidemiological analysis of this genus was studied. Eighty-one strains and 18 random primers were tested. Among the tested primers, the primers NK51 (GGTGGTGGTATC) and NK6 (CCCGCGCCCC) produced n...

journal_title：Journal of clinical microbiology

authors： Okatani AT,Hayashidani H,Takahashi T,Taniguchi T,Ogawa M,Kaneko K

更新日期：2000-12-01 00:00:00

abstract：:We have developed and validated a nested real-time PCR (NRT-PCR) for the genotyping of Chlamydia trachomatis and used it specifically for the typing of either eight genovars from D to K or three genovars of lymphogranuloma venereum (LGV). The 11 probes used in the NRT-PCR correctly identified the DNA from D to K and L...

journal_title：Journal of clinical microbiology

authors： Jalal H,Stephen H,Alexander S,Carne C,Sonnex C

更新日期：2007-08-01 00:00:00

abstract：:High-dose cefepime-tazobactam (1:1; WCK 4282), a novel antibacterial combination consisting of the β-lactamase inhibitor tazobactam and a fourth-generation cephalosporin, is under clinical development for the treatment of serious Gram-negative infections. A quality control (QC) study was performed to establish disk di...

journal_title：Journal of clinical microbiology

authors： Riedel S,Huband MD,Sader HS,Flamm RK,Jones RN

更新日期：2017-10-01 00:00:00

abstract：:We have studied 221 adults drawn from an impoverished urban population with high human immunodeficiency virus (HIV) seroprevalence (35%) to determine the prevalence of gastroduodenal pathology and its relationship to serological markers of Helicobacter pylori virulence proteins and other potential environmental and im...

journal_title：Journal of clinical microbiology

authors： Fernando N,Holton J,Zulu I,Vaira D,Mwaba P,Kelly P

更新日期：2001-04-01 00:00:00

abstract：:A simple, rapid procedure to separate Pneumocystis carinii obtained by lavage of lungs of steroid-treated rats from rat leukocytes is described. Commercially available monoclonal antibody to rat common leukocyte determinants is used to sediment the leukocytes, resulting in supernatant fluid containing P. carinii cells...

journal_title：Journal of clinical microbiology

authors： Pesanti EL,McCarron BA

更新日期：1988-08-01 00:00:00

abstract：:Decreasing the time to species identification and antibiotic susceptibility determination of strains recovered from patients with bacteremia significantly decreases morbidity and mortality. Herein, we validated a method to identify Gram-negative bacteria directly from positive blood culture medium using the Bruker MAL...

journal_title：Journal of clinical microbiology

authors： Wimmer JL,Long SW,Cernoch P,Land GA,Davis JR,Musser JM,Olsen RJ

更新日期：2012-07-01 00:00:00

abstract：:A patient with a dual Salmonella infection is described. Salmonella group B was recovered from three blood culture sets but was not detected in seven stool cultures. Salmonella group C2 was isolated from three of seven stool cultures but was not recovered from blood cultures. Specific, non-cross-reactive antibodies to...

journal_title：Journal of clinical microbiology

authors： Papasian CJ,Bartholomew WR,Neter E,Amsterdam D

更新日期：1984-09-01 00:00:00

abstract：:PCR-restriction fragment length polymorphism analysis (PRA) using the novel region of the rpoB gene was developed for rapid and precise identification of mycobacteria to the species level. A total of 50 mycobacterial reference strains and 3 related bacterial strains were used to amplify the 360-bp region of rpoB, and ...

journal_title：Journal of clinical microbiology

authors： Lee H,Park HJ,Cho SN,Bai GH,Kim SJ

更新日期：2000-08-01 00:00:00

abstract：:A group of 120 sera from blood donors was screened by complement fixation and commercially available immunofluorescence, solid-phase fluorescence immunoassay, enzyme-linked immunosorbent assay, and indirect hemagglutination tests. Twenty-four of the sera were positive by three or more of the five tests and judged to b...

journal_title：Journal of clinical microbiology

authors： Phipps PH,Grégoire L,Rossier E,Perry E

更新日期：1983-12-01 00:00:00

abstract：:New Delhi metallo-β-lactamase (NDM)-producing bacteria are considered potential global health threats. It is necessary to monitor NDM-1 and its variants in clinical isolates in order to understand the NDM-1 epidemic and the impact of its variants on β-lactam resistance. To reduce the lengthy time needed for cloning an...

journal_title：Journal of clinical microbiology

authors： Huang L,Hu X,Zhou M,Yang Y,Qiao J,Wang D,Yu J,Cui Z,Zhang Z,Zhang XE,Wei H

更新日期：2014-06-01 00:00:00

abstract：:Based on findings in small RNA-sequencing (Seq) data analysis, we developed highly sensitive and specific real-time reverse transcription (RT)-PCR assays with locked nucleic acid probes targeting the abundantly expressed leader sequences of Middle East respiratory syndrome coronavirus (MERS-CoV) and other human corona...

journal_title：Journal of clinical microbiology

authors： Chan JF,Choi GK,Tsang AK,Tee KM,Lam HY,Yip CC,To KK,Cheng VC,Yeung ML,Lau SK,Woo PC,Chan KH,Tang BS,Yuen KY

更新日期：2015-08-01 00:00:00

abstract：:Immune pressure on lentiviruses exerted by cytotoxic T lymphocytes (CTL) selects for virus CTL epitope mutations. Currently employed methods for monitoring emerging CTL epitope mutations rely on the labor-intensive and time-consuming techniques of virus population or clonal sequencing. Here we describe the development...

journal_title：Journal of clinical microbiology

authors： Peyerl FW,Barouch DH,Bazick HS,Manuel E,Letvin NL

更新日期：2005-09-01 00:00:00

abstract：:A recent case report of a child infected with enterohemorrhagic Escherichia coli (EHEC) of serotype O118:H16 in Bavaria, in association with the isolation of a bovine O118 strain on the same farm (A. Weber, H. Klie, H. Richter, P. Gallien, M. Timm, and K. W. Perlberg, Berl. Muench. Tieraerztl. Wochenschr. 110:211-213,...

journal_title：Journal of clinical microbiology

authors： Wieler LH,Busse B,Steinrück H,Beutin L,Weber A,Karch H,Baljer G

更新日期：2000-06-01 00:00:00