Measurement of amikacin in serum by a latex agglutination inhibition test.

Abstract:

:Levels of amikacin in serum were determined in 106 serum specimens by a latex agglutination inhibition card test and by radioimmunoassay (RIA). Linear regression analysis demonstrated a high degree of correlation between the two assays (latex = 0.95 (RIA) + 0.69; r = 0.97). Assay of three control sera containing 7.5, 15, and 30 micrograms of amikacin per ml on 7 separate days showed good reproducibility with a coefficient of variation of 0 to 11.7% for the latex assay compared with 7.01 to 22.2% for RIA. Recovery of amikacin in spiked sera varied between 93 and 108% for the latex assay compared with 90 and 100% for RIA. Because the procedure involves a titer, the latex agglutination inhibition card test produces results which are categorized rather than results which are continuous. However, it is a rapid and specific method for determining amikacin levels in clinical specimens and is particularly useful when processing small numbers of specimens.

journal_name

J Clin Microbiol

authors

Khabbaz RF,Standiford HC,Bernstein D,Nipper HC,Tatem BA,Smalls U,Drusano GL,Caplan E

doi

10.1128/JCM.22.5.699-701.1985

subject

Has Abstract

pub_date

1985-11-01 00:00:00

pages

699-701

issue

5

eissn

0095-1137

issn

1098-660X

journal_volume

22

pub_type

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