Isolation of Pseudomonas putrefaciens in intra-abdominal sepsis.

abstract：:Despite advances in the standardization of quantitative DNAemia tests and efforts to better understand and characterize the performance of reference materials in different assays, it remains unclear how the commutability performance of reference materials is related to intra- and interassay agreement. Building upon pr...

journal_title：Journal of clinical microbiology

authors： Tang L,Su Y,Gu Z,Caliendo AM,Pounds S,Hayden RT

更新日期：2019-01-02 00:00:00

abstract：:A disk method for detecting hippurate hydrolysis by Campylobacter spp. was evaluated and compared with the conventional tube test used at the Centers for Disease Control (CDC) (G.K. Morris and C.M. Patton, p. 302-308, in E.H. Lennette, A. Balows, W.J. Hausler, Jr., and H.J. Shadomy, ed., Manual of Clinical Microbiolog...

journal_title：Journal of clinical microbiology

authors： Cacho JB,Aguirre PM,Hernanz A,Velasco AC

更新日期：1989-02-01 00:00:00

abstract：:Mucosal and systemic immunologic recognition of cagA by Helicobacter pylori-infected individuals is associated with peptic ulcer disease; however, in the laboratory, expression of cagA is subject to artificial conditions which may not accurately reflect the conditions in host tissues. Gastric antral and body biopsy sp...

journal_title：Journal of clinical microbiology

authors： Peek RM Jr,Miller GG,Tham KT,Pérez-Pérez GI,Cover TL,Atherton JC,Dunn GD,Blaser MJ

更新日期：1995-01-01 00:00:00

abstract：:We examined mutations in the dihydropteroate synthase (DHPS) genes of Pneumocystis carinii f. sp. hominis (P. carinii) strains isolated from 24 patients with P. carinii pneumonia (PCP) in Japan. DHPS mutations were identified at amino acid positions 55 and/or 57 in isolates from 6 (25.0%) of 24 patients. The underlyin...

journal_title：Journal of clinical microbiology

authors： Takahashi T,Hosoya N,Endo T,Nakamura T,Sakashita H,Kimura K,Ohnishi K,Nakamura Y,Iwamoto A

更新日期：2000-09-01 00:00:00

abstract：:With improved measles virus (MV) control, the genetic variability of the MV-nucleoprotein hypervariable region (NP-HVR) decreases. Thus, it becomes increasingly difficult to determine the origin of a virus using only this part of the genome. During outbreaks in Europe and Africa, we found MV strains with identical NP-...

journal_title：Journal of clinical microbiology

authors： Kessler JR,Kremer JR,Shulga SV,Tikhonova NT,Santibanez S,Mankertz A,Semeiko GV,Samoilovich EO,Tamfum JJ,Pukuta E,Muller CP

更新日期：2011-02-01 00:00:00

abstract：:The majority of cervical human papillomavirus (HPV) infections in young women are transient, but whether the clearance differs among different HPV genotypes and the different factors predicting genotype-specific clearance are partly unknown. In the Finnish Family HPV Study, 131 of 252 women (mean age, 25.5 years) clea...

journal_title：Journal of clinical microbiology

authors： Louvanto K,Syrjänen KJ,Rintala MA,Grénman SE,Syrjänen SM

更新日期：2010-08-01 00:00:00

abstract：:A DNA microarray platform, based on the nucleotide sequences of the internal transcribed spacer regions (ITS1 and ITS2) of the rRNA gene, was developed to identify 32 fungal pathogens at the species level. The probe sequences were spotted onto polycarbonate slides with a mini-microarray printer, and after the hybridiz...

journal_title：Journal of clinical microbiology

authors： Sturaro LL,Gonoi T,Busso-Lopes AF,Tararam CA,Levy CE,Lyra L,Trabasso P,Schreiber AZ,Kamei K,Moretti ML

更新日期：2018-04-25 00:00:00

abstract：:This study aimed to assess the interlaboratory reproducibility at four university hospital laboratories in the southeast region of France of the Etest technique for the determination of caspofungin (CAS) and amphotericin B (AMB) MICs and to compare it to the CLSI broth microdilution reference method. Consecutive clini...

journal_title：Journal of clinical microbiology

authors： Ranque S,Lachaud L,Gari-Toussaint M,Michel-Nguyen A,Mallié M,Gaudart J,Bertout S

更新日期：2012-07-01 00:00:00

abstract：:Rapidly growing mycobacteria are rarely found in central nervous system infections. We describe a case of polymicrobial infection in a brain abscess including two rapidly growing Mycobacterium species, M. immunogenum and M. llatzerense. The Mycobacterium isolates were distinguishable by molecular methods, and whole-ge...

journal_title：Journal of clinical microbiology

authors： Greninger AL,Langelier C,Cunningham G,Keh C,Melgar M,Chiu CY,Miller S

更新日期：2015-07-01 00:00:00

abstract：:Four of eleven affected dogs died despite aggressive treatment during a 2015 focal outbreak of hemorrhagic gastroenteritis following a stay in a pet housing facility. Routine diagnostic investigations failed to identify a specific cause. Virus isolation from fresh necropsy tissues yielded a calicivirus with sequence h...

journal_title：Journal of clinical microbiology

authors： Renshaw RW,Griffing J,Weisman J,Crofton LM,Laverack MA,Poston RP,Duhamel GE,Dubovi EJ

更新日期：2018-04-25 00:00:00

abstract：:Lyme borreliosis is a tick-borne disease caused by the Borrelia burgdorferisensu lato complex. Bio-Rad Laboratories has developed a fully automated multiplex bead-based assay for the detection of IgM and IgG antibodies to B. burgdorferi The BioPlex 2200 Lyme Total assay exhibits an improved rate of seropositivity in p...

journal_title：Journal of clinical microbiology

authors： Hahm JB,Breneman JW 4th,Liu J,Rabkina S,Zheng W,Zhou S,Walker RP,Kaul R

更新日期：2020-04-23 00:00:00

abstract：:We report a case of Coccidioides thyroiditis in an HIV-infected patient with a history of recent Coccidioides pneumonia but with negative Coccidioides serology determined by enzyme immunoassay at presentation. Diagnosis of Coccidioides thyroiditis was made based on histopathologic examination and culture of thyroid ab...

journal_title：Journal of clinical microbiology

authors： Jinno S,Chang S,Jacobs MR

更新日期：2012-07-01 00:00:00

abstract：:Many bunyaviruses of the Bunyamwera and California serogroups are medically important human pathogens. The development of an effective technique to detect the viruses by using molecular biologic tools, such as PCR, improves not only clinical diagnosis but also virologic surveillance of mosquito vectors in the field. I...

journal_title：Journal of clinical microbiology

authors： Kuno G,Mitchell CJ,Chang GJ,Smith GC

更新日期：1996-05-01 00:00:00

abstract：:A space-saving media table designed to facilitate pouring large quantities of plated media in a small area is described. ...

journal_title：Journal of clinical microbiology

authors： McGonagle LA,Schmidt G,Eng S,Wirsing K

更新日期：1975-07-01 00:00:00

abstract：:The objective of this hospital-based study was to determine the relationship between colonizing and infecting strains of Candida species and Torulopsis glabrata. Surveillance cultures from high-risk patients were paired with subsequent bloodstream isolates. Organisms were typed by using restriction endonuclease digest...

journal_title：Journal of clinical microbiology

authors： Reagan DR,Pfaller MA,Hollis RJ,Wenzel RP

更新日期：1990-12-01 00:00:00

abstract：:As contagious bovine pleuropneumonia (CBPP) is spreading fast in many African countries, there is an increasing demand for rapid and sensitive diagnostic methods that can be used to confirm the initial diagnosis based on clinical symptoms or pathological findings. Two PCR-based diagnostic systems for identification of...

journal_title：Journal of clinical microbiology

authors： Persson A,Pettersson B,Bölske G,Johansson KE

更新日期：1999-12-01 00:00:00

abstract：:Between 1 and 2% of the population in the developed world experiences a nonhealing or chronic wound characterized by an apparent arrest in a stage dominated by inflammatory processes. Lately, research groups have proposed that bacteria might be involved in and contribute to the lack of healing of these wounds. To inve...

journal_title：Journal of clinical microbiology

authors： Kirketerp-Møller K,Jensen PØ,Fazli M,Madsen KG,Pedersen J,Moser C,Tolker-Nielsen T,Høiby N,Givskov M,Bjarnsholt T

更新日期：2008-08-01 00:00:00

abstract：:Etest is a new concept for MIC determinations for antimicrobial agents that is based on a predefined antibiotic gradient on a plastic strip calibrated with a continuous logarithmic MIC scale covering 15 twofold dilutions. Etest was compared with a reference agar dilution method for susceptibility testing of clinical i...

journal_title：Journal of clinical microbiology

authors： Hoffner SE,Klintz L,Olsson-Liljequist B,Bolmström A

更新日期：1994-08-01 00:00:00

abstract：:This study was undertaken to develop an accurate, yet inexpensive, method for determining whether the bowel of a neutropenic patient is colonized with bacteria resistant to the antimicrobial agents used in empiric therapy. Selective agar media were prepared in which Mueller-Hinton agar or MacConkey agar were supplemen...

journal_title：Journal of clinical microbiology

authors： Smith JA,Sherlock CH,Burdge DR

更新日期：1984-12-01 00:00:00

abstract：:The performance of a commercially available, rapid membrane enzyme immunoassay for influenza A and B virus detection was compared to that of viral culture in 4,092 respiratory specimens collected from patients presenting with respiratory symptoms during the 2002-2003 influenza season. The test's overall sensitivity wa...

journal_title：Journal of clinical microbiology

authors： Cazacu AC,Chung SE,Greer J,Demmler GJ

更新日期：2004-08-01 00:00:00

abstract：:The object of this study was to develop a test that would measure antibodies directed against group B streptococcal types Ia, Ib, Ic, II, and III. The type-specific carbohydrate antigens were purified, labeled with 125I, and used to develop a radioimmunoassay. This procedure should be particularly useful in testing hu...

journal_title：Journal of clinical microbiology

authors： Wilkinson HW,Jones WL

更新日期：1976-05-01 00:00:00

abstract：:A newly developed commercial serological test (Iatron Laboratories, Inc., Tokyo, Japan) for the rapid identification of medically important species of Candida was evaluated against the API 20C (Analytab Products, Plainview, N.Y.) and the standard Wickerham assimilation and fermentation procedures. Our results indicate...

journal_title：Journal of clinical microbiology

authors： Shinoda T,Kaufman L,Padhye AA

更新日期：1981-03-01 00:00:00

abstract：:On 24 August 2020, the Centers for Disease Control and Prevention (CDC) updated its website to highlight that asymptomatic individuals, even those with exposure to a COVID-19-positive contact, do not necessarily need to be tested unless they have medical conditions associated with increased risk of severe illness from...

journal_title：Journal of clinical microbiology

authors： Schuetz AN,Hemarajata P,Mehta N,Campbell S,Mitchell S,Palavecino E,Butler-Wu S,Miller MB

更新日期：2020-12-17 00:00:00

abstract：:Eighty-seven strains of Pseudomonas aeruginosa were typed by random amplification of polymorphic DNA (RAPD) and pulsed-field gel electrophoresis (PFGE) of macrorestriction fragments. Stains were clustered on the basis of interpretative criteria as presented previously for the PFGE analysis. Clusters of strains were al...

journal_title：Journal of clinical microbiology

authors： Renders N,Römling U,Verbrugh H,van Belkum A

更新日期：1996-12-01 00:00:00

abstract：:A commercial DNA probe kit designed to detect rRNA from legionellae was evaluated for its ability to correctly discriminate between legionellae and non-legionellae taken from culture plates. The probe kit, made by the Gen-Probe Corp. (San Diego, Calif.), was radiolabeled with 125I, and probe bacterial RNA hybridizatio...

journal_title：Journal of clinical microbiology

authors： Edelstein PH

更新日期：1986-03-01 00:00:00

abstract：:A new method for determining the MICs for Aspergillus species with slant-shaking incubation is described. The method was rapid (24 h), produced sharp visual end points, and showed high reproducibility. Additionally, MICs measured by this method correlated well with, as well as were two- to sixfold lower than, those by...

journal_title：Journal of clinical microbiology

authors： Yamada H,Kohno S,Maesaki S,Koga H,Kaku M,Hara K,Tanaka H

更新日期：1993-04-01 00:00:00

abstract：:A three-step method for the purification of Enterocytozoon bieneusi spores from stool specimens was developed. The primary process of purification of the spores from bacterial contaminants involved Percoll gradient centrifugation followed by additional separation using cesium chloride density gradient centrifugation. ...

journal_title：Journal of clinical microbiology

authors： Kucerova Z,Moura H,Leitch GJ,Sriram R,Bern C,Kawai V,Vargas D,Gilman RH,Ticona E,Vivar A,Visvesvara GS

更新日期：2004-07-01 00:00:00

abstract：:The clinical observation has been made that there might be an unacceptable number of false-positive enzyme immunoassay (EIA) test results for IgM among persons suspected of having coccidioidomycosis. Patients with a positive result for IgM by EIA are thought to have a diagnosis of acute coccidioidomycosis. However, th...

journal_title：Journal of clinical microbiology

authors： Kuberski T,Herrig J,Pappagianis D

更新日期：2010-06-01 00:00:00

abstract：:The genus Corynebacterium is a heterogeneous group of species comprising human and animal pathogens and environmental bacteria. It is defined on the basis of several phenotypic characters and the results of DNA-DNA relatedness and, more recently, 16S rRNA gene sequencing. However, the 16S rRNA gene is not polymorphic ...

journal_title：Journal of clinical microbiology

authors： Khamis A,Raoult D,La Scola B

更新日期：2004-09-01 00:00:00

abstract：:Fimbriae of Porphyromonas gingivalis are filamentous appendages on the cell surface and are thought to be one of the virulence factors. The fimA gene encoding the subunit protein of fimbriae, fimbrillin (FimA), was classified into four typeable variants (types I to IV). We previously examined the distribution of P. gi...

journal_title：Journal of clinical microbiology

authors： Nakagawa I,Amano A,Kimura RK,Nakamura T,Kawabata S,Hamada S

更新日期：2000-05-01 00:00:00