Abstract：
:Between 1 and 2% of the population in the developed world experiences a nonhealing or chronic wound characterized by an apparent arrest in a stage dominated by inflammatory processes. Lately, research groups have proposed that bacteria might be involved in and contribute to the lack of healing of these wounds. To investigate this, we collected and examined samples from chronic wounds obtained from 22 different patients, all selected because of suspicion of Pseudomonas aeruginosa colonization. These wound samples were investigated by standard culturing methods and peptide nucleic acid-based fluorescence in situ hybridization (PNA FISH) for direct identification of bacteria. By means of the culturing methods, Staphylococcus aureus was detected in the majority of the wounds, whereas P. aeruginosa was observed less frequently. In contrast, using PNA FISH, we found that a large fraction of the wounds contained P. aeruginosa. Furthermore, PNA FISH revealed the structural organization of bacteria in the samples. It appeared that P. aeruginosa aggregated as microcolonies imbedded in the matrix component alginate, which is a characteristic hallmark of the biofilm mode of growth. The present investigation suggests that bacteria present within these wounds tend to be aggregated in microcolonies imbedded in a self-produced matrix, characteristic of the biofilm mode of growth. Additionally, we must conclude that there exists no good correlation between bacteria detected by standard culturing methods and those detected by direct detection methods such as PNA FISH. This strongly supports the development of new diagnostic and treatment strategies for chronic wounds.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Kirketerp-Møller K,Jensen PØ,Fazli M,Madsen KG,Pedersen J,Moser C,Tolker-Nielsen T,Høiby N,Givskov M,Bjarnsholt Tdoi
10.1128/JCM.00501-08subject
Has Abstractpub_date
2008-08-01 00:00:00pages
2717-22issue
8eissn
0095-1137issn
1098-660Xpii
JCM.00501-08journal_volume
46pub_type
杂志文章abstract：:Nocardia spp. can lead to local or disseminated infections, especially in immunocompromised patients. Combination therapy of amikacin and imipenem is commonly used to treat severe nocardial infections. We describe a patient with imipenem-resistant Nocardia cyriacigeorgica, which, to our knowledge, has not been previou...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.02073-10
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abstract：:We evaluated the performance characteristics of a new, real-time nucleic acid sequence-based amplification (NASBA) assay that incorporates molecular beacon technology for detection of human immunodeficiency virus type 1 (HIV-1). The quantitative results were comparable to those obtained with three leading commercially...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.02187-05
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abstract：:The human herpesvirus 6 (HHV-6) early protein, p41, encoded by the U27 gene has been detected in oligodendrocytes of multiple sclerosis (MS) patients by using a monoclonal antibody (MAb to p41/38). We here report the antigenic epitope of HHV-6 p41 recognized by this MAb. First, we established that the MAb to p41/38 re...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.39.4.1449-1455.2001
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abstract：:The presence of Helicobacter DNA species has been investigated in the biliary epithelium of patients with biliary diseases. However, conflicting results have been observed that may have been due to the small number of subjects studied, difficulty in obtaining a healthy control group, absence of controlling for confoun...
journal_title：Journal of clinical microbiology
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doi：10.1128/jcm.41.12.5615-5618.2003
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abstract：:Tracking the spread of noroviruses during outbreaks of gastroenteritis is hampered by the lack of sequence diversity in those regions of the genome chosen for virus detection and characterization. Sequence analysis of regions of the genes encoding the RNA-dependent RNA polymerase and the S domain of the capsid does no...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.02240-07
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abstract：:An automated enzymatic method was developed for the measurement of D-arabinitol in human serum. The assay is based on a novel, highly specific D-arabinitol dehydrogenase from Candida tropicalis. This enzyme catalyzes the oxidation of D-arabinitol to D-ribulose and the concomitant reduction of NAD+ to NADH. The NADH pr...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.32.1.92-97.1994
更新日期：1994-01-01 00:00:00
abstract：:Selenomonas sputigena is a curved motile anaerobic gram-negative rod that is a part of the normal upper respiratory tract of man. We present a case of speticemia associated with this organism and believe it to be the first such report describing systemic disease. ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.14.6.684-685.1981
更新日期：1981-12-01 00:00:00
abstract：:Skin biopsies of 36 patients with erythema migrans and acrodermatitis chronica atrophicans (ACA) before therapy and those of 8 patients after therapy were examined for Borrelia burgdorferi DNA by PCR. Skin biopsies of 27 patients with dermatological diseases other than Lyme borreliosis and those of 10 healthy persons ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.36.9.2658-2665.1998
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journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.16.2.281-285.1982
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abstract：:Six Food and Drug Administration (FDA)-licensed human immunodeficiency virus type 1 (HIV-1) and HIV-1/2 immunoassays, including five enzyme immunoassays and one rapid test, were challenged with up to 250 serum samples collected from various global sites. The serum samples were from individuals known to be infected wit...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.39.3.1017-1020.2001
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abstract：:A colony blot enzyme-linked immunosorbent assay was developed for the rapid identification of smooth Brucella species, i.e., Brucella abortus, B. melitensis, and B. suis. Bacterial colonies from plates were blotted onto nitrocellulose disks, lysed by immersion in chloroform, and reacted with BRU 38, a rat monoclonal a...
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doi：10.1128/JCM.25.11.2090-2093.1987
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journal_title：Journal of clinical microbiology
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journal_title：Journal of clinical microbiology
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journal_title：Journal of clinical microbiology
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doi：10.1128/JCM.39.2.740-742.2001
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abstract：:Human immunodeficiency virus type 1 serotype C was found in 545 of 712 Ethiopian patients by peptide enzyme immunoassay. Serotyping failed in 146 samples due to the absence of V3 antibodies or multiple reactivities. In 6 of 34 samples, discordant results were obtained by serotyping and genotyping, possibly due to dive...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.35.9.2419-2421.1997
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journal_title：Journal of clinical microbiology
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doi：10.1128/JCM.29.2.346-354.1991
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journal_title：Journal of clinical microbiology
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journal_title：Journal of clinical microbiology
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doi：10.1128/JCM.15.3.535-537.1982
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journal_title：Journal of clinical microbiology
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doi：10.1128/JCM.32.7.1639-1643.1994
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journal_title：Journal of clinical microbiology
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journal_title：Journal of clinical microbiology
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doi：10.1128/JCM.33.10.2792-2795.1995
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journal_title：Journal of clinical microbiology
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journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.34.10.2506-2510.1996
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journal_title：Journal of clinical microbiology
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更新日期：2010-11-01 00:00:00
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journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.35.6.1450-1453.1997
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abstract：:Following intranasal administration of live influenza A virus vaccine or parenteral inoculation of inactivated influenza virus vaccine, immunoglobulin antibody to the influenza virus hemagglutinin was detected in nasal wash specimens from adult volunteers. Several observations supported the suggestion that this immuno...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.25.3.559-562.1987
更新日期：1987-03-01 00:00:00
abstract：:A real-time quantitative PCR assay has been developed to measure human herpesvirus 6 (HHV-6) DNA in biological specimens. The assay sensitivity was 10 copies of DNA per well, with a linear dynamic range of 10 to 10(7) copies of HHV-6 DNA. Intra- and interassay variations were, respectively, 0.88 and 0.8% for samples c...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
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更新日期：2002-07-01 00:00:00
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journal_title：Journal of clinical microbiology
pub_type： 杂志文章
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更新日期：2020-11-18 00:00:00
