Rotavirus gastroenteritis and central nervous system (CNS) infection: characterization of the VP7 and VP4 genes of rotavirus strains isolated from paired fecal and cerebrospinal fluid samples from a child with CNS disease.

abstract：:This study shows that an unbiased amplification method applied to equine arteritis virus RNA significantly improves the sensitivity of the real-time reverse transcription-quantitative PCR (RT-qPCR) recommended by the World Organization for Animal Health. Twelve viral RNAs amplified using this method were hybridized on...

journal_title：Journal of clinical microbiology

authors： Hans A,Gaudaire D,Manuguerra JC,Leon A,Gessain A,Laugier C,Berthet N,Zientara S

更新日期：2015-01-01 00:00:00

abstract：:This is the first report in Israel of a cluster of cases of diarrhea associated with the isolation of Escherichia coli O157 in stool samples. E. coli O157:NM (nonmotile), producing verotoxins 1 and 2 was isolated from the stool samples of three infants less than 1 year of age with diarrhea and from a fourth asymptomat...

journal_title：Journal of clinical microbiology

authors： Lerman Y,Cohen D,Gluck A,Ohad E,Sechter I

更新日期：1992-02-01 00:00:00

abstract：:The ability of MicroScan rapid panels (Baxter MicroScan, West Sacramento, Calif.) to detect oxacillin resistance in 92 clinical isolates of Staphylococcus aureus and 103 coagulase-negative staphylococci was evaluated by comparing results with those of MicroScan 24-h MIC panels and, to resolve discrepancies, oxacillin ...

journal_title：Journal of clinical microbiology

authors： Woods GL,LaTemple D,Cruz C

更新日期：1994-04-01 00:00:00

abstract：:In a prospective, multicenter study of 342 blood samples from 187 patients with systemic inflammatory response syndrome, sepsis, or neutropenic fever, a new commercial PCR test (SepsiTest; Molzym) was evaluated for rapid diagnosis of bacteremia. The test comprises a universal PCR from the 16S rRNA gene, with subsequen...

journal_title：Journal of clinical microbiology

authors： Wellinghausen N,Kochem AJ,Disqué C,Mühl H,Gebert S,Winter J,Matten J,Sakka SG

更新日期：2009-09-01 00:00:00

abstract：:Human metapneumovirus (HMPV) was the unique viral pathogen detected by a real-time reverse transcriptase PCR (RT-PCR) assay in 6 (6.4%) of 94 consecutive French children hospitalized for acute bronchiolitis from September 2001 to June 2002. This virus was identified as the third etiological cause of bronchiolitis, aft...

journal_title：Journal of clinical microbiology

authors： Bouscambert-Duchamp M,Lina B,Trompette A,Moret H,Motte J,Andréoletti L

更新日期：2005-03-01 00:00:00

abstract：:For recovery of brucellae from clinical specimens, a lysis-concentration procedure was superior to the procedure of Castañeda (M. R. Castañeda, Proc. Soc. Exp. Biol. Med. 64:114-115, 1947). ...

journal_title：Journal of clinical microbiology

authors： Etemadi H,Raissadat A,Pickett MJ,Zafari Y,Vahedifar P

更新日期：1984-09-01 00:00:00

abstract：:The coelomycete Colletotrichum gloeosporioides was isolated in pure culture from subcutaneous nodules of the left forearm and elbow of a farmer after traumatic injury. To our knowledge, we report the first case involving this fungus as an etiological agent of subcutaneous infection. The in vitro inhibitory activities ...

journal_title：Journal of clinical microbiology

authors： Guarro J,Svidzinski TE,Zaror L,Forjaz MH,Gené J,Fischman O

更新日期：1998-10-01 00:00:00

abstract：:Pulsed-field gel electrophoretic (PFGE) analysis of Helicobacter pylori isolates is not commonly employed because of the inability to compare the typing with other typing systems. We adapted the PFGE analysis for H. pylori by using EcoRI and slightly modified our laboratory methods to improve the typing of isolates (t...

journal_title：Journal of clinical microbiology

authors： Hosaka Y,Irinoda K,Nakano R,Tanabe S,Koizumi W,Saigenji K,Inoue M

更新日期：2005-02-01 00:00:00

abstract：:The presence of Helicobacter DNA species has been investigated in the biliary epithelium of patients with biliary diseases. However, conflicting results have been observed that may have been due to the small number of subjects studied, difficulty in obtaining a healthy control group, absence of controlling for confoun...

journal_title：Journal of clinical microbiology

authors： Silva CP,Pereira-Lima JC,Oliveira AG,Guerra JB,Marques DL,Sarmanho L,Cabral MM,Queiroz DM

更新日期：2003-12-01 00:00:00

abstract：:In a recent genotypic survey of beta-lactam-resistant pneumococci recovered in different areas of United States during 1997, eight clonal types that each represented 3 to 40 isolates accounted for 134 of 144 isolates (G. Gherardi, C. Whitney, R. Facklam, and B. Beall, J. Infect. Dis. 181:216-229, 2000). We determined ...

journal_title：Journal of clinical microbiology

authors： Beall B,Gherardi G,Facklam RR,Hollingshead SK

更新日期：2000-10-01 00:00:00

abstract：:Pulsed-field gel electrophoresis (PFGE) typing was applied to the epidemiological investigation of 20 Serratia marcescens isolates collected from urine specimens of 17 patients and three urinals over a 2-month period. Twenty-five epidemiologically unrelated strains were also tested to determine the discriminatory powe...

journal_title：Journal of clinical microbiology

authors： Shi ZY,Liu PY,Lau YJ,Lin YH,HU BS

更新日期：1997-01-01 00:00:00

abstract：:A reverse transcriptase PCR (RT-PCR) was used for rapid determination of the hemagglutinin (HA) cleavage site sequence, a marker for the virulence potential of avian influenza viruses. When applied to specimens from chickens experimentally infected with either a virulent or an avirulent virus, RT-PCR uniformly detecte...

journal_title：Journal of clinical microbiology

authors： Horimoto T,Kawaoka Y

更新日期：1995-03-01 00:00:00

abstract：:Scedosporium prolificans is a soil saprophyte that is associated with a large variety of infectious processes and with respiratory colonization in immunocompetent and immunocompromised patients. We report the first described case of S. prolificans keratouveitis associated with the intraocular long-term retention of a ...

journal_title：Journal of clinical microbiology

authors： Arthur S,Steed LL,Apple DJ,Peng Q,Howard G,Escobar-Gomez M

更新日期：2001-12-01 00:00:00

abstract：:A Pasteurella multocida species-specific oligonucleotide probe, pmhyb449, targeting 16S rRNA was designed and evaluated by whole-cell hybridization against 22 selected reference strains in animal tissues. It differentiated P. multocida from other bacterial species of the families Pasteurellaceae and Enterobacteriaceae...

journal_title：Journal of clinical microbiology

authors： Mbuthia PG,Christensen H,Boye M,Petersen KM,Bisgaard M,Nyaga PN,Olsen JE

更新日期：2001-07-01 00:00:00

abstract：:Several species within the Burkholderia cepacia complex (BCC) have emerged as significant opportunistic pathogens of patients with cystic fibrosis (CF). BCC infection is typically associated with a poor clinical prognosis and decreased survival. These factors, combined with the existence of highly transmissible epidem...

journal_title：Journal of clinical microbiology

authors： Brown AR,Govan JR

更新日期：2007-06-01 00:00:00

abstract：:Whole-house surveillance for healthcare-associated infection is no longer the recommended practice because of the large personnel time investment required. We developed a computer-based tracking system using microbiologic data as an aid in detecting potential outbreaks of healthcare-associated infections on a hospital...

journal_title：Journal of clinical microbiology

authors： Hacek DM,Cordell RL,Noskin GA,Peterson LR

更新日期：2004-03-01 00:00:00

abstract：:A previously described microbroth kinetic system (J. Meletiadis, J. F. Meis, J. W. Mouton, and P. E. Verweij, J. Clin. Microbiol. 39:478-484, 2001) based on continuous monitoring of changes in the optical density of fungal growth was used to describe turbidimetric growth curves of different filamentous fungi in the pr...

journal_title：Journal of clinical microbiology

authors： Meletiadis J,te Dorsthorst DT,Verweij PE

更新日期：2003-10-01 00:00:00

abstract：:The source of human immunodeficiency virus type 1 (HIV-1) RNA in cerebrospinal fluid (CSF) during HIV-1 infection is uncertain. The sequence heterogeneity of HIV-1 RNA in simultaneous CSF and plasma samples was characterized for five patients at the baseline and during the first week of antiretroviral therapy by two c...

journal_title：Journal of clinical microbiology

authors： Tang YW,Huong JT,Lloyd RM Jr,Spearman P,Haas DW

更新日期：2000-12-01 00:00:00

abstract：:The accuracy of Vitek MS mass spectrometric identifications was assessed for 206 clinically significant isolates of aerobic Gram-positive bacilli representing 20 genera and 38 species. The Vitek MS identifications were correct for 85% of the isolates (56.3% to the species level, 28.6% limited to the genus level), with...

journal_title：Journal of clinical microbiology

authors： Navas M,Pincus DH,Wilkey K,Sercia L,LaSalvia M,Wilson D,Procop GW,Richter SS

更新日期：2014-04-01 00:00:00

abstract：:Meningococcal disease caused by N. meningitidis serogroup B (MenB) has been endemic in Brazil since 1997. In this study, we determined the prevalence of serosubtypes of MenB isolated in 10 Brazilian states and the Federal District during 1997 and 1998 and investigated the extent of PorA VR sequence variation among the...

journal_title：Journal of clinical microbiology

authors： Sacchi CT,Lemos AP,Popovic T,De Morais JC,Whitney AM,Melles CE,Brondi LM,Monteiro LM,Paiva MV,Solari CA,Mayer LW

更新日期：2001-08-01 00:00:00

abstract：:Family members of hepatitis C virus (HCV)-infected hemodialysis patients had a high rate (8.1%) of HCV infection and were often infected by the same genotype of HCV as the renal patients. Renal patients who had a longer history of hemodialysis or more frequent arteriovenous shunt replacement posed a higher risk to the...

journal_title：Journal of clinical microbiology

authors： Ho MS,Yang CS,Chen PJ,Mau YC

更新日期：1994-11-01 00:00:00

abstract：:The presence of endogenous amplification inhibitors in urine may produce false-negative results for the detection of Chlamydia trachomatis nucleic acids by tests such as PCR, ligase chain reaction (LCR), and transcription-mediated amplification (TMA). Consecutive urine specimens from 101 pregnant women and 287 nonpreg...

journal_title：Journal of clinical microbiology

authors： Mahony J,Chong S,Jang D,Luinstra K,Faught M,Dalby D,Sellors J,Chernesky M

更新日期：1998-11-01 00:00:00

abstract：:Blood was cultured in two vacuum bottles containing Columbia broth. Filtered air was admitted to one bottle (aerobic); the unvented bottle was considered anaerobic. Cultures were incubated at 35 C until growth occurred or for at least 7 days. Of 744 organisms isolated, 50% were isolated from both bottles, 30% from the...

journal_title：Journal of clinical microbiology

authors： Blazevic DJ,Stemper JE,Matsen JM

更新日期：1975-02-01 00:00:00

abstract：:Malignant catarrhal fever (MCF) was diagnosed by clinical signs and lesions in five out of six white-tailed deer (Odocoileus virginianus) in a North American zoo. The clinical signs and histopathological lesions in these deer were typical of MCF. Antibody to an epitope conserved among the MCF viruses was detected in t...

journal_title：Journal of clinical microbiology

authors： Li H,Dyer N,Keller J,Crawford TB

更新日期：2000-04-01 00:00:00

abstract：:Chronic periodontitis is a common infectious disease in the adult population. The etiology is clearly bacterial, and a small number of bacterial species have been consistently associated with periodontitis, including Bacteroides forsythus and Porphyromonas gingivalis. Comparatively little attention has been paid to th...

journal_title：Journal of clinical microbiology

authors： Leys EJ,Lyons SR,Moeschberger ML,Rumpf RW,Griffen AL

更新日期：2002-03-01 00:00:00

abstract：:PCR detecting the protein D (hpd) and fuculose kinase (fucK) genes showed high sensitivity and specificity for identifying Haemophilus influenzae and differentiating it from H. haemolyticus. Phylogenetic analysis using the 16S rRNA gene demonstrated two distinct groups for H. influenzae and H. haemolyticus. ...

journal_title：Journal of clinical microbiology

authors： Theodore MJ,Anderson RD,Wang X,Katz LS,Vuong JT,Bell ME,Juni BA,Lowther SA,Lynfield R,MacNeil JR,Mayer LW

更新日期：2012-04-01 00:00:00

abstract：:Whole-genome sequencing (WGS) is now routinely performed in clinical microbiology laboratories to assess isolate relatedness. With appropriately developed analytics, the same data can be used for prediction of antimicrobial susceptibility. We assessed WGS data for identification using open-source tools and antibiotic ...

journal_title：Journal of clinical microbiology

authors： Ferreira I,Beisken S,Lueftinger L,Weinmaier T,Klein M,Bacher J,Patel R,von Haeseler A,Posch AE

更新日期：2020-06-24 00:00:00

abstract：:A total of 46 Borrelia burgdorferi sensu lato isolates that were isolated from patients with Lyme borreliosis and infected animals or were extracted from ticks of the genus Ixodes were analyzed. Large restriction fragment patterns obtained after cleavage of genomic DNAs with MluI were analyzed by pulsed-field gel elec...

journal_title：Journal of clinical microbiology

authors： Belfaiza J,Postic D,Bellenger E,Baranton G,Girons IS

更新日期：1993-11-01 00:00:00

abstract：:The risk factors for relapse of methicillin-resistant Staphylococcus aureus (MRSA) bacteremia after vancomycin treatment are unknown. Diversilab typing was used to classify recurrent bacteremia as relapse or reinfection. Bacteremia for >7 days and staphylococcal cassette chromosome mec element (SCCmec) type II were in...

journal_title：Journal of clinical microbiology

authors： Welsh KJ,Skrobarcek KA,Abbott AN,Lewis CT,Kruzel MC,Lewis EM,Gardiner JM,Mohr JF,Armitige LY,Wanger A

更新日期：2011-10-01 00:00:00

abstract：:Specific antibodies against human immunodeficiency virus (HIV), usually used for diagnosis, almost invariably become detectable within 3 months of exposure. We report on a patient whose HIV infection was identified early by a combined antigen/antibody test, but seroconversion did not occur for 7 months, until the impl...

journal_title：Journal of clinical microbiology

authors： Chin BS,Lee SH,Kim GJ,Kee MK,Suh SD,Kim SS

更新日期：2007-05-01 00:00:00