Identification of aerobic Gram-positive bacilli by use of Vitek MS.

abstract：:The cellular fatty acid compositions and ubiquinone contents of 182 Legionella strains representing 23 species were determined by capillary gas-liquid chromatography and reverse-phase high-performance liquid chromatography, respectively. Except for the type strain of Legionella erythra (ATCC 35303T), all Legionella sp...

journal_title：Journal of clinical microbiology

authors： Lambert MA,Moss CW

更新日期：1989-03-01 00:00:00

abstract：:Sequence analysis of an 850-bp fragment internal to the aspecific drug efflux gene ade B revealed 11 sequence types (STs) among a collection of 50 multidrug-resistant Acinetobacter baumannii (MDRAB) strains, including members of pan-European clones I, II, and III. The delineation of STs conformed with the intraspecifi...

journal_title：Journal of clinical microbiology

authors： Huys G,Cnockaert M,Nemec A,Swings J

更新日期：2005-10-01 00:00:00

abstract：:Actinomyces viscosus is a gram-positive, non-acid-fact, facultative, catalase-positive, filamentous, or diphtheroidal microorganism. It was isolated from six canine infections during a period of 1.5 years. The organism was cultured from exudate and flaky granules aspirated from infectious granulomas and empyemas. All ...

journal_title：Journal of clinical microbiology

authors： Davenport AA,Carter GR,Patterson MJ

更新日期：1975-01-01 00:00:00

abstract：:This study evaluated the quantitative results from and quality of the inoculation patterns of urine specimens produced by two automated instruments, the Copan WASP and the BD InoqulA. Five hundred twenty-six urine samples submitted in 10-ml canisters containing boric acid were processed within 30 min on an InoqulA ins...

journal_title：Journal of clinical microbiology

authors： Iversen J,Stendal G,Gerdes CM,Meyer CH,Andersen CØ,Frimodt-Møller N

更新日期：2016-02-01 00:00:00

abstract：:We compared examination of unstained and Gram-stained smears as methods of evaluating sputum quality. Of 100 slides examined, 96 (96%) were graded identically. Each discrepancy between methods was near the cutoff for specimen acceptability. We concluded that examination of unstained smears can provide an accurate, cos...

journal_title：Journal of clinical microbiology

authors： Wetterau LM,Zeimis RT,Hollick GE

更新日期：1986-07-01 00:00:00

abstract：:Characterization of 102 Haemophilus strains by the Minitek differentiation system (BBL Microbiology Systems, Cockeysville, Md.) was compared with that by the conventional method. The results obtained by the two methods were in good agreement (P less than 0.001; test of independence). Therefore, this system was found t...

journal_title：Journal of clinical microbiology

authors： Kawakami Y,Okimura Y,Kanai M

更新日期：1981-11-01 00:00:00

abstract：:A modified CAMP test was used to identify 973 Vibrio cholerae isolates by phenotype. Eltor and non-O1 strains were CAMP positive; classical strains were CAMP negative. Sausage-shaped zones of hemolysis of eltor strains were easily distinguished from narrower bands of non-O1 isolates. For O1 isolates, there was 100% ag...

journal_title：Journal of clinical microbiology

authors： Lesmana M,Subekti D,Tjaniadi P,Pazzaglia G

更新日期：1994-01-01 00:00:00

abstract：:The epidemiology of 278 strains of Salmonella typhimurium isolated from 1973 to 1981 from animals in New York State was studied by using four "fingerprinting" techniques, bacteriophage type (B.R. Callow, J. Hyg. 57:346-359, 1959), biotype (J. P. Duguid, E. S. Anderson, G. A. Alfredsson, R. Barker, and D. C. Old, J. Me...

journal_title：Journal of clinical microbiology

authors： McDonough PL,Timoney JF,Jacobson RH,Khakhria R

更新日期：1989-04-01 00:00:00

abstract：:Microscopic examination of a permanently stained fecal preparation revealed the unusual inclusion of a Giardia lamblia cyst within the cytoplasm of an Entamoeba coli trophozoite. ...

journal_title：Journal of clinical microbiology

authors： Logan AL,Turner WE,Price J Jr

更新日期：1980-10-01 00:00:00

abstract：:We evaluated the use of mannitol salt agar with oxacillin for use as a primary screening medium for the simultaneous detection and identification of methicillin-resistant Staphylococcus aureus in clinical surveillance specimens. Oxacillin agar dilution susceptibility tests with mannitol salt agar and Mueller-Hinton ag...

journal_title：Journal of clinical microbiology

authors： Lally RT,Ederer MN,Woolfrey BF

更新日期：1985-10-01 00:00:00

abstract：:We evaluated the Xpert MRSA/SA SSTI real-time PCR assay (Cepheid, Sunnyvale, CA) directly on perioperative bone and joint samples. The sensitivity and specificity for detection of methicillin-susceptible Staphylococcus aureus (MSSA), methicillin-resistant Staphylococcus aureus (MRSA), and methicillin-resistant coagula...

journal_title：Journal of clinical microbiology

authors： Dubouix-Bourandy A,de Ladoucette A,Pietri V,Mehdi N,Benzaquen D,Guinand R,Gandois JM

更新日期：2011-12-01 00:00:00

abstract：:Rapid identification and differentiation of orthopoxviruses by PCR were achieved with primers based on genome sequences encoding the hemagglutinin (HA) protein, an infected-cell membrane antigen that distinguishes orthopoxviruses from other poxvirus genera. The initial identification step used a primer pair of consens...

journal_title：Journal of clinical microbiology

authors： Ropp SL,Jin Q,Knight JC,Massung RF,Esposito JJ

更新日期：1995-08-01 00:00:00

abstract：:HIV-testing algorithms for preexposure prophylaxis (PrEP) should be optimized to minimize the risk of drug resistance, the time off PrEP required to evaluate false-positive screening results, and costs and to expedite the start of therapy for those confirmed to be infected. HIV rapid tests (RTs) for anti-HIV antibodie...

journal_title：Journal of clinical microbiology

authors： Guanira JV,Leigler T,Kallas E,Schechter M,Sharma U,Glidden D,Grant RM,iPrEx Study Team.

更新日期：2015-01-01 00:00:00

abstract：:Helicobacter pylori was grown in low numbers from the saliva of one of nine patients who were positive for gastric H. pylori. The saliva-derived isolate from this patient was identical to the antral biopsy-derived isolate from the same patient and differed from isolates cultured from the antral biopsies of all other p...

journal_title：Journal of clinical microbiology

authors： Ferguson DA Jr,Li C,Patel NR,Mayberry WR,Chi DS,Thomas E

更新日期：1993-10-01 00:00:00

abstract：:Several G8P[6] and G8P[8] rotavirus strains were isolated from hospitalized patients in the Democratic Republic of Congo in 2003. To investigate their overall genomic relatedness and to determine to which genogroup they belonged, the complete genomes of strains DRC88 (G8P[8]) and DRC86 (G8P[6]) were determined. Genomi...

journal_title：Journal of clinical microbiology

authors： Matthijnssens J,Rahman M,Yang X,Delbeke T,Arijs I,Kabue JP,Muyembe JJ,Van Ranst M

更新日期：2006-05-01 00:00:00

abstract：:The identification of Pasteurella and related bacteria remains a challenge. Here, a 449- to 473-bp fragment (sodA(int)) internal to the sodA gene, encoding the manganese-dependent superoxide dismutase, was amplified and sequenced with a single pair of degenerate primers from the type strains of Pasteurella (18 strains...

journal_title：Journal of clinical microbiology

authors： Gautier AL,Dubois D,Escande F,Avril JL,Trieu-Cuot P,Gaillot O

更新日期：2005-05-01 00:00:00

abstract：:We compared 75 nontypeable (NT) Haemophilus influenzae isolates by pulsed-field gel electrophoresis (PFGE), enterobacterial repetitive intergenic consensus (ERIC)-PCR, and automated ribotyping. PFGE was the most discriminatory of the techniques. ERIC-PCR provides a useful screen but should not replace other techniques...

journal_title：Journal of clinical microbiology

authors： Pettigrew MM,Foxman B,Ecevit Z,Marrs CF,Gilsdorf J

更新日期：2002-02-01 00:00:00

abstract：:A survey in 2000 to detect methicillin-resistant Staphylococcus aureus (MRSA) colonization in Vancouver downtown east side injection drug users (IDUs) revealed an MRSA nasal colonization incidence of 7.4%. This is a follow-up study to determine the current prevalence of MRSA colonization and to further characterize th...

journal_title：Journal of clinical microbiology

authors： Al-Rawahi GN,Schreader AG,Porter SD,Roscoe DL,Gustafson R,Bryce EA

更新日期：2008-02-01 00:00:00

abstract：:A serotyping scheme for Enterobacter cloacae based on heat-stable somatic antigens is described. A total of 28 antisera were prepared in rabbits, and titers of agglutinins were high (greater than 640). Some cross-reactions were observed, and 11 sera required absorption before routine use. Of 300 clinical isolates from...

journal_title：Journal of clinical microbiology

authors： Gaston MA,Bucher C,Pitt TL

更新日期：1983-11-01 00:00:00

abstract：:An in situ DNA hybridization kit for cytomegalovirus (CMV) was evaluated for the detection of CMV in centrifugation culture. Of 61 clinical specimens, 17 (27.8%) were positive for CMV by monoclonal antibody staining following centrifugation. Of the 17 positive specimens, 15 were detected by DNA hybridization (24.5%). ...

journal_title：Journal of clinical microbiology

authors： Gleaves CA,Hursh DA,Rice DH,Meyers JD

更新日期：1989-01-01 00:00:00

abstract：:Total immunoglobulin E (IgE) and specific IgE were assayed by a radioimmunosorbent test and a reverse enzyme immunoassay in patients with hookworm infections before and after treatment. A total of 77 subjects (30 patients with hookworm infections and 47 subjects as controls) were studied. Both specific and total IgE l...

journal_title：Journal of clinical microbiology

authors： Ganguly NK,Mahajan RC,Sehgal R,Shetty P,Dilawari JB

更新日期：1988-04-01 00:00:00

abstract：:During a 6-month study we critically evaluated the accuracy of the AutoMicrobic system Gram-Negative Identification Card (Vitek Systems, Inc., Hazelwood, Mo.) in identifying glucose-nonfermenting gram-negative bacilli by testing 419 selected isolates in parallel with a conventional reference method. Of 356 isolates in...

journal_title：Journal of clinical microbiology

authors： Plorde JJ,Gates JA,Carlson LG,Tenover FC

更新日期：1986-02-01 00:00:00

abstract：:In this study, disk diffusion testing with ceftizoxime and cefuroxime was evaluated for use in predicting the susceptibility of Streptococcus pneumoniae to ceftriaxone and cefotaxime. Of the 194 isolates included in this study, 138 were susceptible, 34 were intermediate, and 22 were resistant to cefotaxime by MIC test...

journal_title：Journal of clinical microbiology

authors： Williams-Bouyer N,Hernandez A,Reisner BS

更新日期：1999-11-01 00:00:00

abstract：:The causative agent of Johne's disease is Mycobacterium avium subsp. paratuberculosis. This is a chronic, debilitating gastrointestinal disorder that affects ruminants and is responsible for significant economic loss. The specimen processing method that combines C(18)-carboxypropylbetaine (CB-18) treatment and lytic e...

journal_title：Journal of clinical microbiology

authors： Thornton CG,MacLellan KM,Stabel JR,Carothers C,Whitlock RH,Passen S

更新日期：2002-05-01 00:00:00

abstract：:A multiplex method using the SNaPshot technique was developed to screen for six common mycobacterial species: Mycobacteria tuberculosis, M. avium, M. intracellulare, M. chelonae, M. kansasii, and M. gordonae. A total of 468 mycobacterial clinical isolates were subjected to analysis for the presence of the six mycobact...

journal_title：Journal of clinical microbiology

authors： Wang H,Yue J,Han M,Yang J,Zhao Y

更新日期：2010-01-01 00:00:00

abstract：:Meningococcal disease is normally suspected on clinical grounds and is confirmed by isolation of Neisseria meningitidis bacteria from blood or cerebrospinal fluid or, more recently, by serology or PCR of cerebrospinal fluid. Achieving confirmation of a clinical diagnosis of meningococcal disease has become more diffic...

journal_title：Journal of clinical microbiology

authors： Newcombe J,Cartwright K,Palmer WH,McFadden J

更新日期：1996-07-01 00:00:00

abstract：:Family members of hepatitis C virus (HCV)-infected hemodialysis patients had a high rate (8.1%) of HCV infection and were often infected by the same genotype of HCV as the renal patients. Renal patients who had a longer history of hemodialysis or more frequent arteriovenous shunt replacement posed a higher risk to the...

journal_title：Journal of clinical microbiology

authors： Ho MS,Yang CS,Chen PJ,Mau YC

更新日期：1994-11-01 00:00:00

abstract：:Spontaneous chylothorax is rare in adults. We present an unusual case that was complicated by Prevotella bivia empyema. Full recovery was achieved with chest tube drainage and prompt treatment with intravenous clindamycin. ...

journal_title：Journal of clinical microbiology

authors： Di Marco Berardino A,Inchingolo R,Smargiassi A,Re A,Torelli R,Fiori B,d'Inzeo T,Corbo GM,Valente S,Sanguinetti M,Spanu T

更新日期：2014-04-01 00:00:00

abstract：:We have been characterizing monoclonal antibodies against Norovirus (Norwalk-like virus). In the course of our study, two monoclonal antibodies generated against Norovirus genogroup II capsid protein were found to react not only to genogroup II but also to genogroup I recombinant capsid proteins. In addition, we showe...

journal_title：Journal of clinical microbiology

authors： Yoda T,Suzuki Y,Terano Y,Yamazaki K,Sakon N,Kuzuguchi T,Oda H,Tsukamoto T

更新日期：2003-06-01 00:00:00

abstract：:The herpes simplex virus type 1 (HSV-1) genome is a linear double-stranded DNA of 152 kpb. It is divided into long and short regions of unique sequences termed U(L) and U(S), respectively, and these are flanked by regions of inverted internal and terminal repeats. Microsatellites are short tandem repeats of 1- to 6-nu...

journal_title：Journal of clinical microbiology

authors： Deback C,Boutolleau D,Depienne C,Luyt CE,Bonnafous P,Gautheret-Dejean A,Garrigue I,Agut H

更新日期：2009-03-01 00:00:00