Cellular fatty acid compositions and isoprenoid quinone contents of 23 Legionella species.

Abstract:

:The cellular fatty acid compositions and ubiquinone contents of 182 Legionella strains representing 23 species were determined by capillary gas-liquid chromatography and reverse-phase high-performance liquid chromatography, respectively. Except for the type strain of Legionella erythra (ATCC 35303T), all Legionella species contained large (40 to 90%) amounts of branched-chain fatty acids and only trace to small (less than 0.5 to 5%) amounts of ester-linked hydroxy acids. The 23 species were placed in three major fatty acid groups on the basis of differences in the relative amounts of 14-methylpentadecanoic (Ci16:0), hexadecanoic (C16:1), and 12-methyltetradecanoic (Ca15:0) acids. All Legionella species contained ubiquinones with 9 to 14 isoprene units in the side chains and were divided into five different ubiquinone groups. The species were further differentiated into 16 groups on the basis of qualitative and quantitative differences in their fatty acid compositions and ubiquinone contents. Both of these chemical characteristics can be used to distinguish Legionella species from other gram-negative bacteria and rapidly and accurately identify suspected isolates before serologic and other tests are done.

journal_name

J Clin Microbiol

authors

Lambert MA,Moss CW

doi

10.1128/JCM.27.3.465-473.1989

subject

Has Abstract

pub_date

1989-03-01 00:00:00

pages

465-73

issue

3

eissn

0095-1137

issn

1098-660X

journal_volume

27

pub_type

杂志文章
  • Effectiveness of cephalosporins in the sputum of patients with nosocomial bronchopneumonia.

    abstract::Nosocomial bronchopneumonia is a frequent complication in patients with chronic intratracheal intubation. Despite targeted antibiotic treatment, production of abundant bronchial secretion containing pathogen bacteria often tends to be chronic, and so mortality drastically increases. This problem led to an investigatio...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00893-06

    authors: Klekner A,Bagyi K,Bognar L,Gaspar A,Andrasi M,Szabo J

    更新日期:2006-09-01 00:00:00

  • Occurrence of carbapenem-resistant Acinetobacter baumannii clones at multiple hospitals in London and Southeast England.

    abstract::From late 2003 to the end of 2005, the Health Protection Agency's national reference laboratories received approximately 1,600 referrals of Acinetobacter spp., including 419 and 58 examples, respectively, of two carbapenem-resistant Acinetobacter baumannii lineages, designated OXA-23 clones 1 and 2. Representatives of...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00699-06

    authors: Coelho JM,Turton JF,Kaufmann ME,Glover J,Woodford N,Warner M,Palepou MF,Pike R,Pitt TL,Patel BC,Livermore DM

    更新日期:2006-10-01 00:00:00

  • Bartonella henselae and Bartonella elizabethae as potential canine pathogens.

    abstract::Bartonella henselae or Bartonella elizabethae DNA from EDTA-anticoagulated blood samples obtained from four dogs was amplified and sequenced. The results showed that B. elizabethae should be added to the list of Bartonella species (i.e., B. vinsonii subsp. berkhoffii, B. henselae, and B. clarridgeiae) that are current...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.40.12.4670-4674.2002

    authors: Mexas AM,Hancock SI,Breitschwerdt EB

    更新日期:2002-12-01 00:00:00

  • Single-nucleotide polymorphisms in Rv2629 are specific for Mycobacterium tuberculosis genotypes Beijing and Ghana but not associated with rifampin resistance.

    abstract::Sequence analysis of 58 multidrug-resistant Mycobacterium tuberculosis complex strains from Germany and 55 susceptible strains from a reference collection comprising major phylogenetic lineages confirmed that variations in Rv2629, 191A/C and 965C/T, are specific for genotypes Beijing and Ghana, respectively, but not i...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01237-08

    authors: Homolka S,Köser C,Archer J,Rüsch-Gerdes S,Niemann S

    更新日期:2009-01-01 00:00:00

  • Description of Mycobacterium conceptionense sp. nov., a Mycobacterium fortuitum group organism isolated from a posttraumatic osteitis inflammation.

    abstract::A nonpigmented rapidly growing mycobacterium was isolated from wound liquid outflow, bone tissue biopsy, and excised skin tissue from a 31-year-old woman who suffered an accidental open right tibia fracture and prolonged stay in a river. The three isolates grew in 3 days at 24 to 37 degrees C. 16S rRNA sequence analys...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.44.4.1268-1273.2006

    authors: Adékambi T,Stein A,Carvajal J,Raoult D,Drancourt M

    更新日期:2006-04-01 00:00:00

  • 16S/23S rRNA intergenic spacer regions for phylogenetic analysis, identification, and subtyping of Bartonella species.

    abstract::Species of the genus Bartonella are currently recognized in growing numbers and are involved in an increasing variety of human diseases, mainly trench fever, Carrion's disease, bacillary angiomatosis, endocarditis, cat scratch disease, neuroretinitis, and asymptomatic bacteremia. Such a wide spectrum of infections mak...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.39.8.2768-2778.2001

    authors: Houpikian P,Raoult D

    更新日期:2001-08-01 00:00:00

  • Imported Klebsiella pneumoniae carbapenemase-producing K. pneumoniae clones in a Greek hospital: impact of infection control measures for restraining their dissemination.

    abstract::The recent emergence of carbapenemase-producing Enterobacteriaceae strains represents a major threat for hospitalized patients. We document the dissemination and control of carbapenemase-producing Klebsiella pneumoniae clones in a Greek hospital. During a 3-year study period (January 2009 to December 2011), carbapenem...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00459-12

    authors: Poulou A,Voulgari E,Vrioni G,Xidopoulos G,Pliagkos A,Chatzipantazi V,Markou F,Tsakris A

    更新日期:2012-08-01 00:00:00

  • Performance of clinical laboratories in South African parasitology proficiency testing surveys between 2004 and 2010.

    abstract::Performance in proficiency testing (PT) schemes is an objective measure of a laboratory's best performance. We examined the performance of participants in two parasitology PT schemes in South Africa from 2004 through 2010. The average rates of acceptable scores over the period were 58% and 66% for the stool and blood ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01308-12

    authors: Poonsamy B,Dini L,Frean J

    更新日期:2012-10-01 00:00:00

  • Performance characteristics of the TRUGENE HIV-1 Genotyping Kit and the Opengene DNA Sequencing System.

    abstract::The TRUGENE HIV-1 Genotyping Kit and OpenGene DNA Sequencing System are designed to sequence the protease (PR)- and reverse transcriptase (RT)-coding regions of human immunodeficiency virus type 1 (HIV-1) pol. Studies were undertaken to determine the accuracy of this assay system in detecting resistance-associated mut...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.41.4.1594-1599.2003

    authors: Kuritzkes DR,Grant RM,Feorino P,Griswold M,Hoover M,Young R,Day S,Lloyd RM Jr,Reid C,Morgan GF,Winslow DL

    更新日期:2003-04-01 00:00:00

  • 16S ribosomal DNA typing for identification of pathogens in patients with bacterial keratitis.

    abstract::The identification of pathogens in patients with bacterial keratitis remains problematic because standard diagnostic tests are negative for 40 to 60% of patients. A cross-sectional study was undertaken to determine if PCR and sequence analysis of 16S ribosomal DNA (rDNA) could be used to detect bacterial pathogens in ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.36.12.3492-3496.1998

    authors: Knox CM,Cevellos V,Dean D

    更新日期:1998-12-01 00:00:00

  • Molecular epidemiology of plasmid spread among extended broad-spectrum beta-lactamase-producing Klebsiella pneumoniae isolates in a pediatric hospital.

    abstract::Over a 12-month period, 43 children in eight different wards of our hospital (Hôpital Robert Debré) were infected or colonized with Klebsiella pneumoniae strains producing extended broad-spectrum beta-lactamases. The epidemiology of the outbreak was studied by a molecular approach including the determination of the be...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.31.2.179-184.1993

    authors: Bingen EH,Desjardins P,Arlet G,Bourgeois F,Mariani-Kurkdjian P,Lambert-Zechovsky NY,Denamur E,Philippon A,Elion J

    更新日期:1993-02-01 00:00:00

  • Novel wide-range quantitative nested real-time PCR assay for Mycobacterium tuberculosis DNA: development and methodology.

    abstract::Previously, we designed an internally controlled quantitative nested real-time (QNRT) PCR assay for Mycobacterium tuberculosis DNA in order to rapidly diagnose tuberculous meningitis. This technique combined the high sensitivity of nested PCR with the accurate quantification of real-time PCR. In this study, we attempt...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01200-07

    authors: Takahashi T,Tamura M,Asami Y,Kitamura E,Saito K,Suzuki T,Takahashi SN,Matsumoto K,Sawada S,Yokoyama E,Takasu T

    更新日期:2008-05-01 00:00:00

  • Sodium polyanethole sulfonate as an inhibitor of activation of complement function in blood culture systems.

    abstract::Sodium polyanethole sulfonate (SPS; trade name, Liquoid) is a constituent in culture media used to grow bacteria from blood samples from patients suspected of bacteremia. SPS prevents the killing of bacteria by innate cellular and humoral factors. We analyzed the effect of SPS on the three complement activation pathwa...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01985-09

    authors: Palarasah Y,Skjoedt MO,Vitved L,Andersen TE,Skjoedt K,Koch C

    更新日期:2010-03-01 00:00:00

  • Evaluation and validation of a PulseNet standardized pulsed-field gel electrophoresis protocol for subtyping Vibrio parahaemolyticus: an international multicenter collaborative study.

    abstract::The pandemic spread of Vibrio parahaemolyticus is an international public health issue. Because of the outbreak potential of the organism, it is critical to establish an internationally recognized molecular subtyping protocol for V. parahaemolyticus that is both rapid and robust as a means to monitor its further sprea...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,多中心研究

    doi:10.1128/JCM.00424-08

    authors: Kam KM,Luey CK,Parsons MB,Cooper KL,Nair GB,Alam M,Islam MA,Cheung DT,Chu YW,Ramamurthy T,Pazhani GP,Bhattacharya SK,Watanabe H,Terajima J,Arakawa E,Ratchtrachenchai OA,Huttayananont S,Ribot EM,Gerner-Smidt P,Swamin

    更新日期:2008-08-01 00:00:00

  • Computer-assisted surveillance for detecting clonal outbreaks of nosocomial infection.

    abstract::Whole-house surveillance for healthcare-associated infection is no longer the recommended practice because of the large personnel time investment required. We developed a computer-based tracking system using microbiologic data as an aid in detecting potential outbreaks of healthcare-associated infections on a hospital...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.42.3.1170-1175.2004

    authors: Hacek DM,Cordell RL,Noskin GA,Peterson LR

    更新日期:2004-03-01 00:00:00

  • Prospective comparison of whole-blood- and plasma-based hepatitis C virus RNA detection systems: improved detection using whole blood as the source of viral RNA.

    abstract::We previously demonstrated that whole blood contains significantly more hepatitis C virus (HCV) RNA than plasma. To validate the whole-blood-based HCV RNA detection method, a prospective comparison of HCV RNA detection in whole blood and plasma from 50 patients with chronic liver disease was undertaken. Whole-blood an...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.37.3.484-489.1999

    authors: Stapleton JT,Klinzman D,Schmidt WN,Pfaller MA,Wu P,LaBrecque DR,Han Jq,Phillips MJ,Woolson R,Alden B

    更新日期:1999-03-01 00:00:00

  • Detection of pathogenic protozoa in the diagnostic laboratory: result reproducibility, specimen pooling, and competency assessment.

    abstract::Stool microscopy as performed in clinical parasitology laboratories is a complex procedure with subjective interpretation. Quality assurance (QA) programs often emphasize proficiency testing as an assessment tool. We describe a result reproducibility assessment tool, which can form part of a broader QA program, and wh...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01666-07

    authors: Libman MD,Gyorkos TW,Kokoskin E,Maclean JD

    更新日期:2008-07-01 00:00:00

  • Comparison of preservation methods for enterotoxigenic Escherichia coli producing heat-labile enterotoxin.

    abstract::Ten strains of enterotoxigenic Escherichia coli producing heat-labile enterotoxin (LT) were preserved under 12 different conditions. After 1 month, 9 months, and 3 years of preservation, the cultures were recovered and examined for LT production. Preservation of the cultures on Dorset Egg Medium at 4 degrees C and pre...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.29.10.2326-2328.1991

    authors: Yoh M,Narita I,Honda T,Miwatani T,Nishibuchi M

    更新日期:1991-10-01 00:00:00

  • Measurement of immunoglobulin M (IgM), IgG, and IgA antibodies against Yersinia enterocolitica by enzyme-linked immunosorbent assay: persistence of serum antibodies during disease.

    abstract::An enzyme-linked immunosorbent assay for the detection and quantitation of human immunoglobulin M (IgM), IgG, and IgA antibodies against Yersinia enterocolitica is described. Formalinized or heat-treated bacteria were adsorbed onto specially designed microcuvettes, and antibodies were allowed to attach to the antigen-...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:

    authors: Granfors K

    更新日期:1979-03-01 00:00:00

  • Determination of the range of antibacterial activity by use of viable counts.

    abstract::The activity of three aminoglycosides and six beta-lactam antibiotics on strains of Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa, Staphylococcus aureus, and enterococci was studied. The minimal inhibitory concentrations (MICs), the minimal bactericidal concentrations (MBCs), and t...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.16.1.70-76.1982

    authors: Lorian V,Atkinson BA

    更新日期:1982-07-01 00:00:00

  • API QuadFERM+ with rapid DNase for identification of Neisseria spp. and Branhamella catarrhalis.

    abstract::The QuadFERM+ system (Analytab Products, Plainview, N.Y.), a 2-h carbohydrate degradation method for the identification of Neisseria spp., was evaluated along with a rapid DNase test for confirmation of Branhamella catarrhalis. QuadFERM+ identified 100% of 82 N. gonorrhoeae and 96% of 54 N. meningitidis strains. The t...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.25.2.203-206.1987

    authors: Janda WM,Zigler KL,Bradna JJ

    更新日期:1987-02-01 00:00:00

  • Multicenter Evaluation of the Etest Gradient Diffusion Method for Ceftolozane-Tazobactam Susceptibility Testing of Enterobacteriaceae and Pseudomonas aeruginosa.

    abstract::Ceftolozane-tazobactam (C/T) is a novel beta-lactam-beta-lactamase inhibitor combination antibiotic approved by the U.S. Food and Drug Administration in 2014 for the treatment of complicated intra-abdominal infections (in combination with metronidazole) and complicated urinary tract infections. In this study, we evalu...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,多中心研究

    doi:10.1128/JCM.00717-18

    authors: Bailey AL,Armstrong T,Dwivedi HP,Denys GA,Hindler J,Campeau S,Traczewski M,Humphries R,Burnham CA

    更新日期:2018-08-27 00:00:00

  • Sensitivities of nasal and rectal swabs for detection of methicillin-resistant Staphylococcus aureus colonization in an active surveillance program.

    abstract::All medical and high-risk surgical patients were screened for methicillin-resistant Staphylococcus aureus colonization over 3.5 years. The sensitivities of nasal and rectal swabs were 68% and 62%, respectively. Naris and open-skin-site swabs detected 467 (74%) of 627 adult carriers identified. Rectal swabs detected an...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00848-08

    authors: Currie A,Davis L,Odrobina E,Waldman S,White D,Tomassi J,Katz KC

    更新日期:2008-09-01 00:00:00

  • First isolation of Legionella gormanii from human disease.

    abstract::Legionella gormanii, previously isolated only from the environment, was grown from the bronchial brush specimen of a patient with pneumonia. The organism was characterized by serologic, biochemical, and DNA hybridization studies. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.26.2.380-381.1988

    authors: Griffith ME,Lindquist DS,Benson RF,Thacker WL,Brenner DJ,Wilkinson HW

    更新日期:1988-02-01 00:00:00

  • Enzyme-linked immunosorbent assay compared with neutralization tests for evaluation of live mumps vaccines.

    abstract::Mumps-specific antibody levels before and after vaccination with live mumps vaccines were determined by enzyme-linked immunosorbent assay (ELISA) and neutralization tests. A correlation was found between neutralization titers and optical density in ELISA. However, postvaccination sera from some vaccinees who failed to...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.19.1.21-25.1984

    authors: Sakata H,Hishiyama M,Sugiura A

    更新日期:1984-01-01 00:00:00

  • Large-scale comparative genomics meta-analysis of Campylobacter jejuni isolates reveals low level of genome plasticity.

    abstract::We have used comparative genomic hybridization (CGH) on a full-genome Campylobacter jejuni microarray to examine genome-wide gene conservation patterns among 51 strains isolated from food and clinical sources. These data have been integrated with data from three previous C. jejuni CGH studies to perform a meta-analysi...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,meta分析

    doi:10.1128/JCM.42.10.4566-4576.2004

    authors: Taboada EN,Acedillo RR,Carrillo CD,Findlay WA,Medeiros DT,Mykytczuk OL,Roberts MJ,Valencia CA,Farber JM,Nash JH

    更新日期:2004-10-01 00:00:00

  • Single primer pair for amplifying segments of distinct Shiga-like-toxin genes by polymerase chain reaction.

    abstract::By using a single synthetic oligonucleotide primer pair in the polymerase chain reaction, we amplified specific Shiga-like-toxin (SLT) gene segments from DNAs of 20 clinical Escherichia coli isolates, irrespective of whether they produce SLT-I, SLT-II, or heretofore uncategorized SLTs. These segments were not detectab...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.27.12.2751-2757.1989

    authors: Karch H,Meyer T

    更新日期:1989-12-01 00:00:00

  • Typing of Legionella pneumophila strains by polymerase chain reaction-mediated DNA fingerprinting.

    abstract::Well-defined Legionella pneumophila strains were analyzed by amplification of variable genomic regions with arbitrary and repeat sequence primers. Clinical and environmental outbreak-related isolates showed closely related amplicon patterns. Eleven strains of unrelated origins displayed 10 distinct patterns. Fingerpri...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.31.8.2198-2200.1993

    authors: van Belkum A,Struelens M,Quint W

    更新日期:1993-08-01 00:00:00

  • Development of a treatment algorithm for streptococci and enterococci from positive blood cultures identified with the Verigene Gram-positive blood culture assay.

    abstract::Seventy-eight blood cultures with a Gram stain result of Gram-positive cocci in pairs and/or chains were evaluated with the Nanosphere Verigene Gram-positive blood culture (BC-GP) assay. The overall concordance of the assay with culture was 89.7% (70/78 cultures), allowing for the development of a targeted treatment a...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01587-13

    authors: Alby K,Daniels LM,Weber DJ,Miller MB

    更新日期:2013-11-01 00:00:00

  • Accuracy of Xpert Ultra in Diagnosis of Pulmonary Tuberculosis among Children in Uganda: a Substudy from the SHINE Trial.

    abstract::Childhood tuberculosis (TB) presents significant diagnostic challenges associated with paucibacillary disease and requires a more sensitive test. We evaluated the diagnostic accuracy of Xpert MTB/RIF Ultra (Ultra) compared to other microbiological tests using respiratory samples from Ugandan children in the SHINE tria...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00410-20

    authors: Ssengooba W,Iragena JD,Nakiyingi L,Mujumbi S,Wobudeya E,Mboizi R,Boulware D,Meya DB,Choo L,Crook AM,Lebeau K,Joloba M,Demers AM,Cresswell FV,Gibb DM

    更新日期:2020-08-24 00:00:00