Measurement of immunoglobulin M (IgM), IgG, and IgA antibodies against Yersinia enterocolitica by enzyme-linked immunosorbent assay: persistence of serum antibodies during disease.

abstract：:The epidemiology of 278 strains of Salmonella typhimurium isolated from 1973 to 1981 from animals in New York State was studied by using four "fingerprinting" techniques, bacteriophage type (B.R. Callow, J. Hyg. 57:346-359, 1959), biotype (J. P. Duguid, E. S. Anderson, G. A. Alfredsson, R. Barker, and D. C. Old, J. Me...

journal_title：Journal of clinical microbiology

authors： McDonough PL,Timoney JF,Jacobson RH,Khakhria R

更新日期：1989-04-01 00:00:00

abstract：:Histoplasmosis is an important systemic fungal infection, particularly among immunocompromised individuals, who may develop a progressive disseminated form which is often fatal if it is untreated. In such patients, the detection of antibody responses for both diagnosis and follow-up may be of limited use, whereas the ...

journal_title：Journal of clinical microbiology

authors： Gómez BL,Figueroa JI,Hamilton AJ,Diez S,Rojas M,Tobón A,Restrepo A,Hay RJ

更新日期：1999-03-01 00:00:00

abstract：:The degree of DNA banding pattern polymorphism exhibited by vancomycin-resistant Enterococcus faecium (VREM) strains isolated on a renal unit over an 11-month period was investigated. Thirty VREM strains from different patients were analyzed by pulsed-field gel electrophoresis (PFGE; with extended run and optimal puls...

journal_title：Journal of clinical microbiology

authors： Morrison D,Woodford N,Barrett SP,Sisson P,Cookson BD

更新日期：1999-04-01 00:00:00

abstract：:Plasmid-mediated colistin resistance (PMCR) is a global public health concern, given its ease of transmissibility. The purpose of this study was to evaluate two methods for the detection of PMCR from bacterial colonies: (i) the NG-Test MCR-1 lateral flow immunoassay (LFA; NG Biotech, Guipry, France) and (ii) the EDTA-...

journal_title：Journal of clinical microbiology

authors： Fenwick AJ,Bergman Y,Lewis S,Yee R,Uhlemann AC,Cole N,Kohner P,Ordak C,Green DA,Schuetz AN,Humphries R,Simner PJ

更新日期：2020-03-25 00:00:00

abstract：:Test strips for the detection of antibodies to human immunodeficiency virus type 1 were investigated using specimens from risk groups in Thailand (141 reactive; 445 nonreactive) in a local Thai laboratory. The diagnostic sensitivity and specificity were both 100%. Using a set of seroconversion panels, the sensitivity ...

journal_title：Journal of clinical microbiology

authors： Pagcharoenpol P,Burgess-Cassler A,Schramm W

更新日期：1996-04-01 00:00:00

abstract：:We have developed a novel high-throughput PCR-ligase detection reaction-capillary electrophoresis (PCR-LDR-CE) assay for the multiplexed identification of 20 blood-borne pathogens (Staphylococcus epidermidis, Staphylococcus aureus, Bacillus cereus, Enterococcus faecalis, Enterococcus faecium, Listeria monocytogenes, S...

journal_title：Journal of clinical microbiology

authors： Pingle MR,Granger K,Feinberg P,Shatsky R,Sterling B,Rundell M,Spitzer E,Larone D,Golightly L,Barany F

更新日期：2007-06-01 00:00:00

abstract：:Rhesus and cynomolgus monkey kidney tissue cultures and two continuous lines, Madin-Darby canine kidney (MDCK) and LLC-MK2, were compared in titrations and isolations of influenza and parainfluenza viruses. Tube cultures were inoculated with laboratory virus strains or stored patient specimens and observed for hemadso...

journal_title：Journal of clinical microbiology

authors： Frank AL,Couch RB,Griffis CA,Baxter BD

更新日期：1979-07-01 00:00:00

abstract：:Anaerobes are an important but uncommon cause of bloodstream infections (BSIs). For pediatric patients, routine inclusion of an anaerobic blood culture alongside the aerobic remains controversial. We implemented automatic anaerobic blood culture alongside aerobic blood cultures in a pediatric emergency department (ED)...

journal_title：Journal of clinical microbiology

authors： Dien Bard J,Chang TP,Yee R,Manshadi K,Lichtenfeld N,Choi HJ,Festekjian A

更新日期：2020-08-24 00:00:00

abstract：:Identification of members of the Mycobacterium tuberculosis complex and the M. avium-M. intracellulare complex (MAC) directly from primary BACTEC cultures was evaluated by using acridinium-ester-labeled DNA probes (AccuProbe; GenProbe, Inc., San Diego, Calif.). In preliminary experiments, blood present in samples was ...

journal_title：Journal of clinical microbiology

authors： Evans KD,Nakasone AS,Sutherland PA,de la Maza LM,Peterson EM

更新日期：1992-09-01 00:00:00

abstract：:An echovirus 3 (Echo3) strain (strain LR31G7) was isolated from a sewage treatment plant in Greece in 2005. Full-genome molecular, phylogenetic, and SimPlot analyses were conducted in order to reveal the evolutionary pathways of the isolate. Nucleotide and phylogenetic analyses of part of the VP1 genomic region reveal...

journal_title：Journal of clinical microbiology

authors： Kyriakopoulou Z,Dedepsidis E,Pliaka V,Tsakogiannis D,Pratti A,Levidiotou-Stefanou S,Markoulatos P

更新日期：2010-05-01 00:00:00

abstract：:Genetic determinants of the invasive phenotype of Shigella spp. and enteroinvasive Escherichia coli (EIEC), two common agents of bacillary dysentery, are encoded on large (180- to 210 kilobase), nonconjugative plasmids. Several plasmid-encoded antigens have been implicated as important bacterial ligands that mediate t...

journal_title：Journal of clinical microbiology

authors： Venkatesan M,Buysse JM,Vandendries E,Kopecko DJ

更新日期：1988-02-01 00:00:00

abstract：:Although Escherichia fergusonii has been identified for decades, it has rarely been recovered from clinical specimens and its clinical significance remains unclear. We describe a case of E. fergusonii bacteremia in a diabetic patient with pancreatic cancer. The isolate was confirmed by three commercial identification ...

journal_title：Journal of clinical microbiology

authors： Lai CC,Cheng A,Huang YT,Chung KP,Lee MR,Liao CH,Hsueh PR

更新日期：2011-11-01 00:00:00

abstract：:We used an enzyme-linked immunosorbent assay (ELISA) of immunoglobulin G avidity to determine the dengue immune status of 105 pairs of serum samples from patients infected with dengue virus. This study shows that a simple avidity test, for which only one acute-phase serum sample is required, is potentially more useful...

journal_title：Journal of clinical microbiology

authors： Matheus S,Deparis X,Labeau B,Lelarge J,Morvan J,Dussart P

更新日期：2005-11-01 00:00:00

abstract：:We evaluated the performance of three commercially available monoclonal antibodies for confirmation of the presence of Chlamydia pneumoniae in cell culture by examining their abilities to stain inclusions of eight strains of C. pneumoniae. The antibodies tested were two unconjugated C. pneumoniae-specific monoclonal r...

journal_title：Journal of clinical microbiology

authors： Montalban GS,Roblin PM,Hammerschlag MR

更新日期：1994-05-01 00:00:00

abstract：:During an outbreak of diarrhea in a general hospital in 1992, 166 Clostridium difficile isolates from 102 patients were typed by restriction enzyme analysis (REA), arbitrarily primed PCR (AP-PCR), and protein profile analysis (PP) techniques. A total of 18 types and 5 subtypes were identified by REA, 32 types were ide...

journal_title：Journal of clinical microbiology

authors： Rafferty ME,Baltch AL,Smith RP,Bopp LH,Rheal C,Tenover FC,Killgore GE,Lyerly DM,Wilkins TD,Schoonmaker DJ,Hannett GE,Shayegani M

更新日期：1998-10-01 00:00:00

abstract：:Multilocus sequence typing was successfully completed on 494 isolates of Streptococcus uberis from clinical mastitis cases in a study of 52 commercial dairy herds over a 12-month period. In total, 195 sequence types (STs) were identified. S. uberis mastitis cases that occurred in different cows within the same herd an...

journal_title：Journal of clinical microbiology

authors： Davies PL,Leigh JA,Bradley AJ,Archer SC,Emes RD,Green MJ

更新日期：2016-01-01 00:00:00

abstract：:Hepatitis G virus (HGV) was recently identified as a new member of the Flaviviridae, but its clinical significance is still unclear. Since no immunoassay for the diagnosis of HGV is available, we developed a sensitive reverse transcription-PCR (RT-PCR) assay to facilitate the detection of the viral genome by mass scre...

journal_title：Journal of clinical microbiology

authors： Schlueter V,Schmolke S,Stark K,Hess G,Ofenloch-Haehnle B,Engel AM

更新日期：1996-11-01 00:00:00

abstract：:Serum samples collected from 68 patients (age, mean +/- the standard deviation [SD], 56.3 +/- 12.8 years) at admission who were subsequently molecularly diagnosed as having hepatitis E and from 2,781 individuals who were assumed not to have been recently infected with hepatitis E virus (HEV; negative controls; 52.9 +/...

journal_title：Journal of clinical microbiology

authors： Takahashi M,Kusakai S,Mizuo H,Suzuki K,Fujimura K,Masuko K,Sugai Y,Aikawa T,Nishizawa T,Okamoto H

更新日期：2005-01-01 00:00:00

abstract：:Duck hepatitis B virus (DHBV) belongs to the Hepadnaviridae family, which includes human Hepatitis B virus (HBV) and Woodchuck hepatitis virus. It is widely distributed in wild and domestic ducks due to congenital transmission. HBV is a worldwide health problem, with carriers at risk of developing cirrhosis and liver ...

journal_title：Journal of clinical microbiology

authors： Wang CY,Giambrone JJ,Smith BF

更新日期：2002-07-01 00:00:00

abstract：:In vitro studies established that neither cloth nor disposable diapers demonstrably contributed to the growth of Escherichia coli, Proteus vulgaris, Staphylococcus aureus, or Candida albicans when urine was present as a growth medium. In a clinical study of 166 children, the microbial skin flora of children with atopi...

journal_title：Journal of clinical microbiology

authors： Keswick BH,Seymour JL,Milligan MC

更新日期：1987-02-01 00:00:00

abstract：:Gas-liquid chromatographic (GLC) profiles of cellular fatty acids and metabolic products were useful in identifying strains of Peptococcus saccharolyticus, Peptococcus asaccharolyticus, Peptostreptococcus anaerobius, Peptostreptococcus micros, and Streptococcus intermedius. The GLC results supported the recent taxonom...

journal_title：Journal of clinical microbiology

authors： Lambert MA,Armfield AY

更新日期：1979-10-01 00:00:00

abstract：:We report on a novel strategy for the detection of mRNA targets derived from Cryptosporidium parvum oocysts by the use of oligonucleotide-gold nanoparticles. Gold nanoparticles are functionalized with oligonucleotides which are complementary to unique sequences present on the heat shock protein 70 (HSP70) DNA/RNA targ...

journal_title：Journal of clinical microbiology

authors： Javier DJ,Castellanos-Gonzalez A,Weigum SE,White AC Jr,Richards-Kortum R

更新日期：2009-12-01 00:00:00

abstract：:Strains of Candida albicans obtained from human immunodeficiency virus (HIV)-positive individuals prior to their first episode of oral thrush were already in a high-frequency mode of switching and were far more resistant to a number of antifungal drugs than commensal isolates from healthy individuals. Switching in the...

journal_title：Journal of clinical microbiology

authors： Vargas K,Messer SA,Pfaller M,Lockhart SR,Stapleton JT,Hellstein J,Soll DR

更新日期：2000-10-01 00:00:00

abstract：:Escherichia coli isolates that cause detachment of cell monolayers during in vitro adherence assays (cell-detaching E. coli [CDEC]) were recently reported as a potential new group of enteropathogenic bacteria. In the present study, 269 E. coli isolates from feces of children 1 to 5 years of age were identified as CDEC...

journal_title：Journal of clinical microbiology

authors： Marques LR,Abe CM,Griffin PM,Gomes TA

更新日期：1995-10-01 00:00:00

abstract：:Levels of amikacin in serum were determined in 106 serum specimens by a latex agglutination inhibition card test and by radioimmunoassay (RIA). Linear regression analysis demonstrated a high degree of correlation between the two assays (latex = 0.95 (RIA) + 0.69; r = 0.97). Assay of three control sera containing 7.5, ...

journal_title：Journal of clinical microbiology

authors： Khabbaz RF,Standiford HC,Bernstein D,Nipper HC,Tatem BA,Smalls U,Drusano GL,Caplan E

更新日期：1985-11-01 00:00:00

abstract：:Lower levels of performance of human papillomavirus (HPV) typing assays in studies using formalin-fixed paraffin-embedded (FFPE) tissue compared to those using exfoliated cervical cells have been reported. The interpretation of current studies is limited by bias in inclusion criteria, sample matching, and methods of c...

journal_title：Journal of clinical microbiology

authors： Dal Bello B,Spinillo A,Alberizzi P,Cesari S,Gardella B,Silini EM

更新日期：2009-07-01 00:00:00

abstract：:Vibrio metschnikovii and Vibrio gazogenes are two new Vibrio species that have been little studied. Thirteen strains of V. metschnikovii were highly related to the type strain, NCTC 8443, by DNA-DNA hybridization. Relatedness values were 83 to 90% at 60 degrees C and 75 to 84% at the more stringent 75 degrees C. Diver...

journal_title：Journal of clinical microbiology

authors： Farmer JJ 3rd,Hickman-Brenner FW,Fanning GR,Gordon CM,Brenner DJ

更新日期：1988-10-01 00:00:00

abstract：:We recently tested serum from a human immunodeficiency virus-infected patient for the presence of cryptococcal antigen using the Meridian latex agglutination (LA) test (Cryptococcal Antigen Latex Agglutination System). Two pronase-treated serum specimens from the patient had LA titers of 80 and 160, but the patient ha...

journal_title：Journal of clinical microbiology

authors： Whittier S,Hopfer RL,Gilligan P

更新日期：1994-09-01 00:00:00

abstract：:Candida samples were taken over a period of 2 years from 54 human immunodeficiency virus (HIV)-positive asymptomatic subjects to evaluate changes in yeast carriage, intensity of carriage, and genotype over time. Overall, we found that HIV-positive patients with CD4(+)-cell counts of between 200 and 400/microl had sign...

journal_title：Journal of clinical microbiology

authors： Vargas KG,Joly S

更新日期：2002-02-01 00:00:00

abstract：:We explored three genes of attachment (fbe and atlE) and adhesion (ica) in 27 and 10 Staphylococcus epidermidis strains involved in pacemaker-related infections (PMI) and intravascular-catheter-related infections (IVCI), respectively, and in 25 saprophytic strains. The detection rates of fbe and atlE were identical in...

journal_title：Journal of clinical microbiology

authors： Klug D,Wallet F,Kacet S,Courcol RJ

更新日期：2003-07-01 00:00:00