Carriage frequency, intensity of carriage, and strains of oral yeast species vary in the progression to oral candidiasis in human immunodeficiency virus-positive individuals.

abstract：:A new compound, indoxyl-beta-D-glucuronide, was assessed as a substrate for the rapid detection of Escherichia coli in urine. Incorporation of this compound into MacConkey agar allowed the direct differentiation of E. coli as deep blue colonies distinct from lactose and nonlactose fermenters. The sensitivity was 88 to...

journal_title：Journal of clinical microbiology

authors： Delisle GJ,Ley A

更新日期：1989-04-01 00:00:00

abstract：:In a cohort of subjects from Italy, anti-hepatitis C virus (HCV) and HCV RNA [HCV(+) subgroup] prevalences were 24.6 and 79.6%, respectively. HCV types 1b and 2a/c accounted for 95% of infections. Adjusted alanine aminotransferase levels were higher in males than in females and in RNA-positive subjects than in RNA-neg...

journal_title：Journal of clinical microbiology

authors： Osella AR,Sonzogni L,Cavallini A,Foti L,Guerra V,Di Leo A,Mondelli MU,Misciagna G,Silini EM

更新日期：1999-07-01 00:00:00

abstract：:We retrospectively compared the utility of a fungal isolation device (Isolator) versus conventional techniques for recovering fungal organisms from blood cultures obtained from neutropenic cancer patients. Positive cultures were deemed true pathogens, possible pathogens, or contaminants according to laboratory and cli...

journal_title：Journal of clinical microbiology

authors： Creger RJ,Weeman KE,Jacobs MR,Morrissey A,Parker P,Fox RM,Lazarus HM

更新日期：1998-01-01 00:00:00

abstract：:Campylobacter jejuni from sporadic cases and outbreaks of gastroenteritis were serotyped on the basis of heat-extracted soluble thermostable antigens identified with the use of the passive hemagglutination technique. A total of 168 isolates were separated into 45 different types. The largest proportion of the isolates...

journal_title：Journal of clinical microbiology

authors： McMyne PM,Penner JL,Mathias RG,Black WA,Hennessy JN

更新日期：1982-08-01 00:00:00

abstract：:Since the discovery of the Norwalk virus (NV) by immune electron microscopy (IEM) in 1972, serologic studies with this virus have relied on particle-positive fecal material from infected volunteers as the source of antigen because it has not been possible to propagate this virus in cell culture. However, the recent cl...

journal_title：Journal of clinical microbiology

authors： Green KY,Lew JF,Jiang X,Kapikian AZ,Estes MK

更新日期：1993-08-01 00:00:00

abstract：:A clustered regularly interspaced short palindromic repeat (CRISPR) typing method has recently been developed and used for typing and subtyping of Salmonella spp., but it is complicated and labor intensive because it has to analyze all spacers in two CRISPR loci. Here, we developed a more convenient and efficient meth...

journal_title：Journal of clinical microbiology

authors： Li H,Li P,Xie J,Yi S,Yang C,Wang J,Sun J,Liu N,Wang X,Wu Z,Wang L,Hao R,Wang Y,Jia L,Li K,Qiu S,Song H

更新日期：2014-08-01 00:00:00

abstract：:We analyzed the characteristics of subjects from the same area who were infected with hepatitis C virus genotypes 1 through 4 and subtypes 1a and 1b. Our data are consistent with a rapid evolution in the epidemiology of HCV genotypes and argue against different pathogenic potentials for genotypes 1b and 2. ...

journal_title：Journal of clinical microbiology

authors： Ravaggi A,Rossini A,Mazza C,Puoti M,Marin MG,Cariani E

更新日期：1996-11-01 00:00:00

abstract：:A multiplex PCR assay for the identification of human diarrheagenic Escherichia coli was developed. The targets selected for each category were eae for enteropathogenic E. coli, stx for Shiga toxin-producing E. coli, elt and est for enterotoxigenic E. coli, ipaH for enteroinvasive E. coli, and aggR for enteroaggregati...

journal_title：Journal of clinical microbiology

authors： Toma C,Lu Y,Higa N,Nakasone N,Chinen I,Baschkier A,Rivas M,Iwanaga M

更新日期：2003-06-01 00:00:00

abstract：:Hepatitis B surface antigen was concentrated and purified from plasma by two simple steps of purification. In the first step the antigen was purified 24-fold by polyethylene glycol precipitation. An additional 10-fold purification was achieved by batchwise adsorption to hydroxylapatite and subsequent elution with 0.02...

journal_title：Journal of clinical microbiology

authors： Vnek J,Prince AM

更新日期：1976-06-01 00:00:00

abstract：:Quantitative PCR on nasopharyngeal aspirate (NPA) can achieve high sensitivity and specificity in diagnosing Pneumocystis pneumonia (PCP) compared to microscopic examination of bronchoscopic specimens in a population with low HIV prevalence. Since NPA is a minimally invasive procedure, it is ideal as a screening test ...

journal_title：Journal of clinical microbiology

authors： To KK,Wong SC,Xu T,Poon RW,Mok KY,Chan JF,Cheng VC,Chan KH,Hung IF,Yuen KY

更新日期：2013-05-01 00:00:00

abstract：:Serological tests to detect Trypanosoma cruzi antibodies have been used for screening blood donors, for epidemic studies, and for diagnosis of probably infected persons. Among different tests, the enzyme-linked immunosorbent assay (ELISA) with total, semipurified, or synthetic antigens has been widely used, mainly due...

journal_title：Journal of clinical microbiology

authors： Ferreira AW,Belem ZR,Lemos EA,Reed SG,Campos-Neto A

更新日期：2001-12-01 00:00:00

abstract：:Coronavirus disease (COVID) serological tests are essential to determine the overall seroprevalence of a population and to facilitate exposure estimates within that population. We performed a head-to-head assessment of enzyme immunoassays (EIAs) and point-of-care lateral flow assays (POCTs) to detect severe acute resp...

journal_title：Journal of clinical microbiology

authors： Charlton CL,Kanji JN,Johal K,Bailey A,Plitt SS,MacDonald C,Kunst A,Buss E,Burnes LE,Fonseca K,Berenger BM,Schnabl K,Hu J,Stokes W,Zelyas N,Tipples G

更新日期：2020-09-22 00:00:00

abstract：:The detection of immunoglobulin M (IgM) antibodies to cytomegalovirus-induced late antigens by an indirect immunofluorescence assay was improved by the use of monoclonal antibodies to human IgM. Nonspecific background fluorescence was absent, facilitating the reading of the slides and the detection of a specific fluor...

journal_title：Journal of clinical microbiology

authors： Zerbini M,Musiani M,Gentilomi G,La Placa M

更新日期：1986-07-01 00:00:00

abstract：:We compared the Mycoplasma Duo kit (Sanofi Diagnostics Pasteur) with PCR for detection of Ureaplasma spp. in endotracheal aspirates from 60 premature neonates. The overall agreement between the two tests was 96%. The Mycoplasma Duo assay is a useful alternative to culture and PCR for detection of neonatal Ureaplasma i...

journal_title：Journal of clinical microbiology

authors： Cheah FC,Anderson TP,Darlow BA,Murdoch DR

更新日期：2005-01-01 00:00:00

abstract：:A colony blot enzyme-linked immunosorbent assay was developed for the rapid identification of smooth Brucella species, i.e., Brucella abortus, B. melitensis, and B. suis. Bacterial colonies from plates were blotted onto nitrocellulose disks, lysed by immersion in chloroform, and reacted with BRU 38, a rat monoclonal a...

journal_title：Journal of clinical microbiology

authors： Roop RM 2nd,Preston-Moore D,Bagchi T,Schurig GG

更新日期：1987-11-01 00:00:00

abstract：:A new oligochromatographic assay, Speed-Oligo Novel Influenza A H1N1, was designed and optimized for the specific detection of the 2009 influenza A H1N1 virus. The assay is based on a PCR method coupled to detection of PCR products by means of a dipstick device. The target sequence is a 103-bp fragment within the hema...

journal_title：Journal of clinical microbiology

authors： Pérez-Ruiz M,Navarro-Marí JM,Bautista-Marín MF,Pedrosa-Corral I,Sanbonmatsu-Gámez S,Camacho AG,Rojas J,Ruiz-Ortiz J,Rodríguez-Granger J,Carrillo JA

更新日期：2010-05-01 00:00:00

abstract：:During 1994, a cross-contamination problem leading to false-positive cultures of Mycobacterium tuberculosis was revealed in a mycobacteriology laboratory processing 30,000 to 35,000 samples per year. Molecular strain typing based on restriction fragment length polymorphism confirmed the contaminations. Out of 1,439 po...

journal_title：Journal of clinical microbiology

authors： Bauer J,Thomsen VO,Poulsen S,Andersen AB

更新日期：1997-04-01 00:00:00

abstract：:The rapid identification of blood culture isolates and antimicrobial susceptibility test (AST) results play critical roles for the optimal treatment of patients with bloodstream infections. Whereas others have looked at the time to detection in automated culture systems, we examined the overall time from specimen coll...

journal_title：Journal of clinical microbiology

authors： Tabak YP,Vankeepuram L,Ye G,Jeffers K,Gupta V,Murray PR

更新日期：2018-11-27 00:00:00

abstract：:Gram stains remain the cornerstone of diagnostic testing in the microbiology laboratory for the guidance of empirical treatment prior to availability of culture results. Incorrectly interpreted Gram stains may adversely impact patient care, and yet there are no comprehensive studies that have evaluated the reliability...

journal_title：Journal of clinical microbiology

authors： Samuel LP,Balada-Llasat JM,Harrington A,Cavagnolo R

更新日期：2016-06-01 00:00:00

abstract：:The fungus Fusarium sacchari was isolated repeatedly from the blood of an immunosuppressed host. The infection was treated successfully with a small dose of amphotericin B. The strain was resistant to this antifungal in vitro. MICs and minimum fungicidal concentrations of six antifungals for the clinical isolate are p...

journal_title：Journal of clinical microbiology

authors： Guarro J,Nucci M,Akiti T,Gené J,Barreiro MD,Gonçalves RT

更新日期：2000-01-01 00:00:00

abstract：:The ability of Staphylococcus aureus to clot plasma through conformational activation of prothrombin by staphylocoagulase is used to distinguish S. aureus from coagulase-negative staphylococci. We show that while the direct thrombin inhibitor dabigatran inhibits staphylocoagulase activity, the clinical use of dabigatr...

journal_title：Journal of clinical microbiology

authors： Vanassche T,Verhaegen J,Peetermans WE,Hoylaerts MF,Verhamme P

更新日期：2010-11-01 00:00:00

abstract：:Fifteen strains of an anaerobic, catalase-negative, gram-positive diphtheroid-shaped bacterium recovered from human sources were characterized by phenotypic and molecular chemical and molecular genetic methods. The unidentified bacterium showed some resemblance to Actinomyces species and related taxa, but biochemical ...

journal_title：Journal of clinical microbiology

authors： Hall V,Collins MD,Lawson PA,Hutson RA,Falsen E,Inganas E,Duerden B

更新日期：2003-02-01 00:00:00

abstract：:The actinomycete Rhodococcus equi is an important pathogen of horses and an emerging opportunistic pathogen of humans. Identification of R. equi by classical bacteriological techniques is sometimes difficult, and misclassification of an isolate is not uncommon. We report here on a specific PCR assay for the rapid and ...

journal_title：Journal of clinical microbiology

authors： Ladrón N,Fernández M,Agüero J,González Zörn B,Vázquez-Boland JA,Navas J

更新日期：2003-07-01 00:00:00

abstract：:An in situ DNA hybridization kit for cytomegalovirus (CMV) was evaluated for the detection of CMV in centrifugation culture. Of 61 clinical specimens, 17 (27.8%) were positive for CMV by monoclonal antibody staining following centrifugation. Of the 17 positive specimens, 15 were detected by DNA hybridization (24.5%). ...

journal_title：Journal of clinical microbiology

authors： Gleaves CA,Hursh DA,Rice DH,Meyers JD

更新日期：1989-01-01 00:00:00

abstract：:To determine that susceptibility of AMPLICOR Chlamydia trachomatis PCR to inhibitory factors possibly present in cervical specimens, we obtained cervical specimens from 200 gynecology patients attending our outpatient clinic. The prevalence of C. trachomatis infection was 4.1%, as determined by cell culture. All AMPLI...

journal_title：Journal of clinical microbiology

authors： Verkooyen RP,Luijendijk A,Huisman WM,Goessens WH,Kluytmans JA,van Rijsoort-Vos JH,Verbrugh HA

更新日期：1996-12-01 00:00:00

abstract：:Automated treponemal immunoassays are used for syphilis screening with the reverse-sequence algorithm; discordant results (e.g., enzyme immunoassay [EIA] reactive and reactive plasma reagin [RPR] nonreactive) are resolved with a second treponemal test. We conducted a study to determine automated immunoassay signal str...

journal_title：Journal of clinical microbiology

authors： Fakile YF,Jost H,Hoover KW,Gustafson KJ,Novak-Weekley SM,Schapiro JM,Tran A,Chow JM,Park IU

更新日期：2017-12-26 00:00:00

abstract：:A semisolid medium (designated Serratia differentiation medium) containing L-arabinose, ornithine, and selective inhibitor was used to differentiate three clinically encountered Serratia species. The inhibitor, Irgasan DP-300, was incorporated to eliminate false-positive reactions from most remaining Enterobacteriacea...

journal_title：Journal of clinical microbiology

authors： Gibson S,Friedman H

更新日期：1978-03-01 00:00:00

abstract：:Interfering substances have been reported to inhibit PCR assays for the direct detection of Mycobacterium tuberculosis in clinical specimens. Using an internal control, we determined that 52% of respiratory specimens interfered with our PCR assay. On the basis of these findings, we tried to circumvent the problem by s...

journal_title：Journal of clinical microbiology

authors： Forbes BA,Hicks KE

更新日期：1996-09-01 00:00:00

abstract：:The molecular epidemiology of tuberculosis (TB) in Finland was studied by spoligotyping 380 Mycobacterium tuberculosis isolates. The isolates were obtained during a 1-year study period from July 2000 to June 2001 and represented 90% of new M. tuberculosis findings by culture in the whole country during the study perio...

journal_title：Journal of clinical microbiology

authors： Puustinen K,Marjamäki M,Rastogi N,Sola C,Filliol I,Ruutu P,Holmström P,Viljanen MK,Soini H

更新日期：2003-04-01 00:00:00

abstract：:In vitro susceptibility patterns of newer beta-lactamase-inhibiting antibiotics ampicillin-sulbactam (A/S) and amoxicillin-clavulanic acid (A/C) for 100 consecutive isolates of Acinetobacter baumannii obtained from various clinical samples were studied. The A/C MIC for 86% of the strains was more than 16/8 microgram/m...

journal_title：Journal of clinical microbiology

authors： Pandey A,Kapil A,Sood S,Goel V,Das B,Seth P

更新日期：1998-11-01 00:00:00