Development and preliminary evaluation of a rapid oligochromatographic assay for specific detection of new human influenza A H1N1 virus.

abstract：:Patients with chronic granulomatous disease have a marked defect in neutrophil oxidative metabolism and microbicidal activity. Asymptomatic mothers of males with the disease can usually be identified as heterozygous carriers by intermediate leukocyte function. Most mothers of females with the disease, however, have no...

journal_title：Journal of clinical microbiology

authors： Mills EL,Rholl KS,Quie PG

更新日期：1980-07-01 00:00:00

abstract：:Mycobacterium avium subsp. paratuberculosis is the causative agent of Johne's disease (JD) in cattle and may be associated with Crohn's disease (CD) in humans. It is the slowest growing of the cultivable mycobacteria, and culture from clinical, veterinary, food, or environmental specimens can take 4 months or even lon...

journal_title：Journal of clinical microbiology

authors： Imirzalioglu C,Dahmen H,Hain T,Billion A,Kuenne C,Chakraborty T,Domann E

更新日期：2011-05-01 00:00:00

abstract：:Multilocus variable-number tandem-repeat analysis (MLVA) for seven genomic loci was developed for Enterococcus faecalis. MLVA and pulsed-field gel electrophoresis (PFGE) resulted in 37 and 31 genotypes among 83 strains, respectively. Both typing schemes were highly concordant (90.4%). MLVA is an excellent alternative ...

journal_title：Journal of clinical microbiology

authors： Titze-de-Almeida R,Willems RJ,Top J,Rodrigues IP,Ferreira RF 2nd,Boelens H,Brandileone MC,Zanella RC,Felipe MS,van Belkum A

更新日期：2004-10-01 00:00:00

abstract：:Statistical analysis of a limiting dilution assay (LDA) showed that the occurrence of infectious human immunodeficiency virus type 1 (HIV-1)-harboring cells in serially diluted samples of peripheral blood mononuclear cells (PBMCs) of HIV-1-seropositive patients fits the model describing a single-hit Poisson distributi...

journal_title：Journal of clinical microbiology

authors： Lu W,Manolikakis G,Andrieu JM

更新日期：1992-10-01 00:00:00

abstract：:Two hundred seventy nasopharyngeal aspirates were tested in duplicate with the Ortho Diagnostics, Inc. (Raritan, N.J.), respiratory syncytial virus antigen enzyme-linked immunosorbent assay. The test was sensitive (80 to 82%) and specific (96%) when compared with cell culture. The enzyme-linked immunosorbent assay det...

journal_title：Journal of clinical microbiology

authors： Bromberg K,Tannis G,Daidone B,Clarke L,Sierra MF

更新日期：1985-12-01 00:00:00

abstract：:Seventy-six nontuberculous mycobacterial isolates obtained from patients living in Greece were analyzed with the GenoType Mycobacterium CM (for common mycobacteria) and AS (for additional species) assays. GenoType correctly identified all but one of the mycobacterial species. For this species, additional probes should...

journal_title：Journal of clinical microbiology

authors： Gitti Z,Neonakis I,Fanti G,Kontos F,Maraki S,Tselentis Y

更新日期：2006-06-01 00:00:00

abstract：:The IS200-like insertion sequence (IS) is a 708-bp element recently found in Yersinia pestis. Its nucleotide sequence is 85% identical to that of IS200 recovered in most Salmonella enterica isolates. It is also present in multiple copies in Y. pseudotuberculosis. In contrast, this IS is found in some (biotype 1B strai...

journal_title：Journal of clinical microbiology

authors： Odaert M,Berche P,Simonet M

更新日期：1996-09-01 00:00:00

abstract：:Chlamydia trachomatis was recoved from 20% (36/180) of women attending a venereal disease clinic. All infected women had chlamydial antibodies in their serum and cervical secretions. However, the background rates of chlamydial antibody in chlamydia-negative women were very high. Measurement of antibodies in serum (com...

journal_title：Journal of clinical microbiology

authors： Schachter J,Cles L,Ray R,Hines PA

更新日期：1979-11-01 00:00:00

abstract：:Molecular typing has become indispensable in the detection of nosocomial transmission of bacterial pathogens and the identification of sources and routes of transmission in outbreak settings, but current methods are labor-intensive, are difficult to standardize, or have limited resolution. Whole-genome multilocus sequ...

journal_title：Journal of clinical microbiology

authors： Kluytmans-van den Bergh MF,Rossen JW,Bruijning-Verhagen PC,Bonten MJ,Friedrich AW,Vandenbroucke-Grauls CM,Willems RJ,Kluytmans JA

更新日期：2016-12-01 00:00:00

abstract：:Community-acquired bloodstream infections cause substantial morbidity and mortality worldwide, but microbiology capacity and surveillance limitations have challenged good descriptions of pathogen distribution in many regions, including Southeast Asia. Active surveillance for bloodstream infections has been conducted i...

journal_title：Journal of clinical microbiology

authors： Jorakate P,Higdon M,Kaewpan A,Makprasert S,Yuenprakhon S,Tawisaid K,Dejsirilert S,Whistler T,Baggett HC

更新日期：2015-03-01 00:00:00

abstract：:A quantitative nucleic acid sequence-based amplification (QT-NASBA) assay for the detection of Plasmodium parasites has been developed. Primers and probes were selected on the basis of the sequence of the small-subunit rRNA gene. Quantification was achieved by coamplification of the RNA in the sample with one modified...

journal_title：Journal of clinical microbiology

authors： Schoone GJ,Oskam L,Kroon NC,Schallig HD,Omar SA

更新日期：2000-11-01 00:00:00

abstract：:Members of the genus Malassezia, lipophilic yeasts, are considered to be one of the exacerbating factors in atopic dermatitis (AD). We examined variation in cutaneous colonization by Malassezia species in AD patients and compared it with variation in healthy subjects. Samples were collected by applying transparent dre...

journal_title：Journal of clinical microbiology

authors： Sugita T,Suto H,Unno T,Tsuboi R,Ogawa H,Shinoda T,Nishikawa A

更新日期：2001-10-01 00:00:00

abstract：:A total of 1,298 nonfermentative gram-negative rods were used to evaluate the performance of the individual biochemical tests in the N/F System and to determine whether the observed results included sufficient key reactions for rapid identification. The system included an oxidase test, two screening tubes providing 5 ...

journal_title：Journal of clinical microbiology

authors： Barnishan J,Ayers LW

更新日期：1979-02-01 00:00:00

abstract：:Quantitative HIV RNA viral load (QVL) assays (Roche Diagnostics) were sensitive and specific when used to diagnose HIV infection in (i) HIV-exposed infants (sensitivity of 100% [63.1 to 100%] and specificity of 100% [97.9 to 100%]) and (ii) suspected acute HIV infection patients with a negative/indeterminate Western b...

journal_title：Journal of clinical microbiology

authors： Lee BE,Plitt SS,Jayaraman GC,Chui L,Singh AE,Preiksaitis JK

更新日期：2012-02-01 00:00:00

abstract：:A combination of immunomagnetic separation (IMS) and a polymerase chain reaction (PCR) procedure was used for direct isolation and identification of Shigella dysenteriae type 1 and Shigella flexneri from feces. Immunomagnetic particles were coated with monoclonal antibody MASFB, which is specific for a common epitope ...

journal_title：Journal of clinical microbiology

authors： Islam D,Lindberg AA

更新日期：1992-11-01 00:00:00

abstract：:The current article summarizes recent changes in nomenclature for fungi of medical importance published in the years 2018 to 2019, including new species and revised names for existing ones. Many of the revised names have been widely adopted without further discussion. However, those that concern common pathogens of hu...

journal_title：Journal of clinical microbiology

authors： Borman AM,Johnson EM

更新日期：2021-01-21 00:00:00

abstract：:We investigated the presence of Chlamydia pneumoniae in 81 normal and pathological specimens obtained from postmortem brain tissues of patients with multiple sclerosis and with other neurological or nonneurological diseases. The assays used included PCR amplification of all DNA samples in the initial study. Culture an...

journal_title：Journal of clinical microbiology

authors： Hammerschlag MR,Ke Z,Lu F,Roblin P,Boman J,Kalman B

更新日期：2000-11-01 00:00:00

abstract：:Aspirates have been cultured from 10 dental abscesses of endodontic origin, all of which had penetrated beyond the bony alveolus to produce fluctuant swelling. Sampling was by syringe aspiration. Strict anaerobic techniques, including the use of an anaerobic chamber, were used for serial dilution and plating. Randomly...

journal_title：Journal of clinical microbiology

authors： Williams BL,McCann GF,Schoenknecht FD

更新日期：1983-10-01 00:00:00

abstract：:A set of 104 isolates from human clinical samples from the United States, morphologically compatible with Bipolaris, were morphologically and molecularly identified through the sequence analysis of the internal transcribed space (ITS) region of the nuclear ribosomal DNA (rDNA). The predominant species was Bipolaris sp...

journal_title：Journal of clinical microbiology

authors： da Cunha KC,Sutton DA,Fothergill AW,Cano J,Gené J,Madrid H,De Hoog S,Crous PW,Guarro J

更新日期：2012-12-01 00:00:00

abstract：:The clinical significance of viridans streptococci isolated from the blood cultures of 86 patients was determined. Isolates that were significant or suggestive of infection represented only 21% of the cases. Among 54 isolates for which the species was known, Streptococcus sanguis II was the most common. However, a sig...

journal_title：Journal of clinical microbiology

authors： Swenson FJ,Rubin SJ

更新日期：1982-04-01 00:00:00

abstract：:In a multicenter study, the consistency of international units expressed by five commercially available rubella virus immunoglobulin G kits was evaluated. The linearity and within-run and between-run precision were determined for each kit. All kits demonstrated good linearity and had within-run and between-run precisi...

journal_title：Journal of clinical microbiology

authors： Dimech W,Bettoli A,Eckert D,Francis B,Hamblin J,Kerr T,Ryan C,Skurrie I

更新日期：1992-03-01 00:00:00

abstract：:This report describes the development and evaluation of an enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to Entamoeba histolytica. Highly sensitive and reproducible results were obtained in antigen-coated plates prepared by air-drying at 37 degrees C. Comparison of the ELISA with indirect f...

journal_title：Journal of clinical microbiology

authors： Yang J,Kennedy MT

更新日期：1979-12-01 00:00:00

abstract：:Twelve Salmonella typhimurium strains resistant to broad-spectrum cephalosporins were isolated from cases of gastroenteritis during 1996 to 1998 in Russia, Hungary, and Greece. Resistance was due to the production of CTX-M-type extended-spectrum beta-lactamases encoded by similar 12-kb plasmids. By pulsed-field gel el...

journal_title：Journal of clinical microbiology

authors： Tassios PT,Gazouli M,Tzelepi E,Milch H,Kozlova N,Sidorenko S,Legakis NJ,Tzouvelekis LS

更新日期：1999-11-01 00:00:00

abstract：:Aeromonas caviae was recovered as the sole potential enteric pathogen from the stools of 14 of 17 symptomatic children (10 younger than 1 year of age) while Aeromonas hydrophila, Aeromonas sobria, and Plesiomonas shigelloides were isolated once each. The infants from whom A. caviae was isolated all presented with a wa...

journal_title：Journal of clinical microbiology

authors： Namdari H,Bottone EJ

更新日期：1990-05-01 00:00:00

abstract：:Hepatitis C virus (HCV) genotype 4 (HCV-4) infection is considered to be difficult to treat and has become increasingly prevalent in European countries, including The Netherlands. Using a molecular epidemiological approach, the present study investigates the genetic diversity and evolutionary origin of HCV-4 in Amster...

journal_title：Journal of clinical microbiology

authors： de Bruijne J,Schinkel J,Prins M,Koekkoek SM,Aronson SJ,van Ballegooijen MW,Reesink HW,Molenkamp R,van de Laar TJ

更新日期：2009-12-01 00:00:00

abstract：:One hundred ninety-three Streptococcus agalactiae isolates of neonatal origin and 146 isolates from adult women were analyzed for macrolide resistance and investigated for clonality. Among erythromycin-resistant isolates, serotype V turned out to be the most frequent. Comparative pulsed-field gel electrophoresis analy...

journal_title：Journal of clinical microbiology

authors： von Both U,Ruess M,Mueller U,Fluegge K,Sander A,Berner R

更新日期：2003-05-01 00:00:00

abstract：:Indirect and double-antibody sandwich enzyme-linked immunosorbent assay techniques were compared for serotyping 42 herpes simplex virus isolates. Both procedures gave similar results, which were in complete agreement with those obtained by inhibition of the indirect hemagglutination test. The indirect technique proved...

journal_title：Journal of clinical microbiology

authors： Gerna G,Battaglia M,Revello MG,Gerna MT

更新日期：1983-05-01 00:00:00

abstract：:The DNA relatedness and restriction fragment length polymorphism patterns of whole-cell DNA and the general phenotypic properties of 14 isolates of the form species Candida parapsilosis representing three diverse genetic groups were compared. The data confirm the unrelatedness of the three groups at the species level....

journal_title：Journal of clinical microbiology

authors： Roy B,Meyer SA

更新日期：1998-01-01 00:00:00

abstract：:The coagulation of Limulus amebocyte lysate (LAL) can be activated through two pathways, one initiated by endotoxin and the other by beta-glucans. The two pathways join at the step of activation of the proclotting enzyme. We report here that the endotoxin-activated pathway can be differentially inhibited by two method...

journal_title：Journal of clinical microbiology

authors： Zhang GH,Baek L,Buchardt O,Koch C

更新日期：1994-06-01 00:00:00

abstract：:Based on 16S rRNA gene PCR, no significant difference was observed in rates of detection of Helicobacter species in intestinal biopsy specimens from 160 Chinese inflammatory bowel disease (IBD) patients (10%) and 80 controls (6.3%). By using a [(13)C]urea breath test, the H. pylori infection rate in 208 Chinese IBD pa...

journal_title：Journal of clinical microbiology

authors： Zhang S,Zhong B,Chao K,Xiao Y,Cui Y,Gao X,Chen B,He Y,Hu P,Chen M,Mitchell HM

更新日期：2011-05-01 00:00:00