Isolation of Helicobacter pylori from saliva.

abstract：:We report an uncommon clinical presentation of a unique case of fatal invasive fungal cerebral vasculitis due to Arthrographis kalrae in a nonimmunocompromised host. The identity of the fungus was determined by morphological characteristics and by analysis of internal transcribed spacer 1 sequences and was confirmed b...

journal_title：Journal of clinical microbiology

authors： Pichon N,Ajzenberg D,Desnos-Ollivier M,Clavel M,Gantier JC,Labrousse F

更新日期：2008-09-01 00:00:00

abstract：:We report an outbreak of Saccharomyces cerevisiae subtype boulardii fungemia among three intensive care unit roommates of patients receiving lyophilized preparations of this fungus. The fungemia was probably due to central venous catheter contamination and resolved after fluconazole treatment. The need for stringent a...

journal_title：Journal of clinical microbiology

authors： Cassone M,Serra P,Mondello F,Girolamo A,Scafetti S,Pistella E,Venditti M

更新日期：2003-11-01 00:00:00

abstract：:The frequency with which clue cells could be detected in Gram-stained vaginal smears and/or cervical Papanicolaou (Pap) smears was compared with the frequency of Corynebacterium vaginale (Haemophilus vaginalis) isolation in a group of 236 female patients, of whom 221 had vaginitis. Vaginal clue cells were found most o...

journal_title：Journal of clinical microbiology

authors： Smith RF,Rodgers HA,Hines PA,Ray RM

更新日期：1977-03-01 00:00:00

abstract：:Capnocytophaga canimorsus is a dog oral commensal that causes rare but severe infections in humans. C. canimorsus was recently shown to be endowed with a capsular polysaccharide implicated in resistance to the innate immune system of the host. Here, we developed the first C. canimorsus capsular serotyping scheme. We d...

journal_title：Journal of clinical microbiology

authors： Hess E,Renzi F,Koudad D,Dol M,Cornelis GR

更新日期：2017-06-01 00:00:00

abstract：:A rapid method for the detection of acetylneuraminyl hydrolase, EC 3.2.1.18 (sialidase or neuraminidase), was developed by using 2'-(4-methylumbelliferyl)alpha-D-N-acetylneuraminic acid as substrate in a filter paper spot test. The method was compared to conventional assays that use 2'-(4-methylumbelliferyl)alpha-D-N-...

journal_title：Journal of clinical microbiology

authors： Moncla BJ,Braham P

更新日期：1989-01-01 00:00:00

abstract：:The genetic relatedness of Streptococcus milleri group isolates from the airways of cystic fibrosis patients was determined by using pulsed-field gel electrophoresis. This study reveals no evidence for patient-to-patient transmission in our patient population; however, within individual patients, complex inter- and in...

journal_title：Journal of clinical microbiology

authors： Sibley CD,Sibley KA,Leong TA,Grinwis ME,Parkins MD,Rabin HR,Surette MG

更新日期：2010-07-01 00:00:00

abstract：:Two previously demonstrated sensitive and specific enzyme immunoassays (EIAs) for rotavirus, one using polyclonal and monoclonal antisera (TestPack Rotavirus [TPK]; Abbott Laboratories) and the other using only monoclonal anti-rotavirus antibodies (Rotaclone [RTC]; Cambridge BioScience Corporation), were evaluated as ...

journal_title：Journal of clinical microbiology

authors： Dennehy PH,Gauntlett DR,Spangenberger SE

更新日期：1990-06-01 00:00:00

abstract：:Typical and atypical Campylobacter strains were isolated from the colons of cattle and swine by techniques that enabled the selective pressures of antibiotics to be avoided. Some cephalothin-susceptible strains and a strain with an indeterminate hippurate reaction were classified as Campylobacter coli by DNA homology ...

journal_title：Journal of clinical microbiology

authors： Ng LK,Taylor DE,Stiles ME

更新日期：1988-03-01 00:00:00

abstract：:Bordetella pertussis was diagnosed in a human immunodeficiency virus-infected patient by a newly developed method in which bacterial DNA is amplified directly from sputum Gram-stained slides. The validation of the method is described along with an additional new PCR-based assay that can distinguish between B. pertussi...

journal_title：Journal of clinical microbiology

authors： Vielemeyer O,Crouch JY,Edberg SC,Howe JG

更新日期：2004-02-01 00:00:00

abstract：:In contrast to cervical cancer, integration of human papillomavirus (HPV) DNA into the host genome has been considered a rare event in cancer precursor lesions (cervical intraepithelial neoplasia [CIN]). With our new real-time PCR method, we demonstrated that integrated HPV type 16 (HPV16) is already present in CIN le...

journal_title：Journal of clinical microbiology

authors： Peitsaro P,Johansson B,Syrjänen S

更新日期：2002-03-01 00:00:00

abstract：:Testing for susceptibility to pyrazinamide (PZA) and analysis of the pncA gene sequences of 423 Mycobacterium tuberculosis complex isolates have revealed a unique silent nucleotide substitution that enables the rapid identification of "M. canettii" (proposed name). Moreover, the lack of a defined mutation within the p...

journal_title：Journal of clinical microbiology

authors： Somoskovi A,Dormandy J,Parsons LM,Kaswa M,Goh KS,Rastogi N,Salfinger M

更新日期：2007-02-01 00:00:00

abstract：:Freshly isolated clinical strains of Klebsiella were tested for the ability to fix dinitrogen by the acetylene reduction assay. Ability to detect this trait was markedly affected by cultural conditions. When the test was run at 37 degrees C in the presence of yeast extract (50 mg/liter), only 1.6% of the organisms wer...

journal_title：Journal of clinical microbiology

authors： Chambers CA,Silver WS

更新日期：1977-11-01 00:00:00

abstract：:Invasive collection methods are often required to obtain samples for the microbiological evaluation of children with presumptive pulmonary tuberculosis (PTB). Nucleic acid amplification testing of easier-to-collect stool samples could be a noninvasive method of diagnosing PTB. We conducted a systematic review and meta...

journal_title：Journal of clinical microbiology

authors： MacLean E,Sulis G,Denkinger CM,Johnston JC,Pai M,Ahmad Khan F

更新日期：2019-05-24 00:00:00

abstract：:Clinically important species of Candida and related organisms were compared for extent of nucleotide divergence in the 5' end of the large-subunit (26S) ribosomal DNA (rDNA) gene. This rDNA region is sufficiently variable to allow reliable separation of all known clinically significant yeast species. Of the 204 descri...

journal_title：Journal of clinical microbiology

authors： Kurtzman CP,Robnett CJ

更新日期：1997-05-01 00:00:00

abstract：:An isolate of Proteus mirabilis recovered from blood cultures of a diabetic patient was shown to be resistant to imipenem, meropenem, and ertapenem by disk diffusion susceptibility testing. Amplification of whole-cell and/or plasmid DNA recovered from the isolate with primers specific for the bla(KPC) carbapenemase ge...

journal_title：Journal of clinical microbiology

authors： Tibbetts R,Frye JG,Marschall J,Warren D,Dunne W

更新日期：2008-09-01 00:00:00

abstract：:Investigations of nursery outbreaks of Citrobacter diversus sepsis and meningitis have been hampered by lack of adequate epidemiologic markers for the organism. We studied outer membrane protein profiles from clinical isolates of C. diversus by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to determine whe...

journal_title：Journal of clinical microbiology

authors： Kline MW,Mason EO Jr,Kaplan SL

更新日期：1989-08-01 00:00:00

abstract：:Bartonella henselae uveitis was diagnosed in a 40-year-old woman with underlying HLA-B27 uveitis on the basis of immunodetection and molecular detection of the organism, a Marseilles genogroup and a CAL-1 genotype strain, in the vitreous fluid. This case illustrates that B. henselae should be included in the different...

journal_title：Journal of clinical microbiology

authors： Drancourt M,Bodaghi B,Lepidi H,Le Hoang P,Raoult D

更新日期：2004-04-01 00:00:00

abstract：:Group B streptococci (GBS) cause severe invasive disease in both neonates and adults. Understanding the epidemiology of GBS provides information that can include determining disease prevalence rates in defined populations and geographic regions, documenting the success of GBS screening programs, and understanding anti...

journal_title：Journal of clinical microbiology

authors： Alhhazmi A,Hurteau D,Tyrrell GJ

更新日期：2016-07-01 00:00:00

abstract：:The results of single radial immunodiffusion assays of influenza virus hemagglutinin were found to be greatly altered by small antigenic differences between test and reference strains. When such differences were present, the precise specificity of the antiserum used had a critical effect on the measured hemagglutinin ...

journal_title：Journal of clinical microbiology

authors： Rodda SJ,Gallichio HA,Hampson AW

更新日期：1981-11-01 00:00:00

abstract：:Noroviruses are the leading cause of outbreaks of gastroenteritis in the world. At present, norovirus genogroup II, genotype 4 (GII/4), strains are the most prevalent in many countries. In this study we investigated 55 outbreaks and 35 sporadic cases of norovirus-associated gastroenteritis in food handlers in food-cat...

journal_title：Journal of clinical microbiology

authors： Ozawa K,Oka T,Takeda N,Hansman GS

更新日期：2007-12-01 00:00:00

abstract：:The CO2, O2, and pH levels of commercially available blood culture bottles with tryptic soy broth medium from five different manufacturers were compared. Ranges of 1.3 to 6.9% for CO2, 1.1 to 6.0% for O2, and pH 6.94 to 7.26 were found. Different venting procedures revealed that blood culture bottles from which the ru...

journal_title：Journal of clinical microbiology

authors： Welch WD,Porschen RK,Zarifi SZ

更新日期：1984-11-01 00:00:00

abstract：:Two bacteriologic systems for detecting bacteria in blood were compared; the automated radiometric BACTEC and the conventional method used in our laboratory for many years. BACTEC consisted of two bottles with 30 ml and the conventional method with 50 ml of media for aerobes and anaerobes. The BACTEC bottles were inoc...

journal_title：Journal of clinical microbiology

authors： Thiemke WA,Wicher K

更新日期：1975-03-01 00:00:00

abstract：:The effectiveness of antiretroviral therapy may be limited by the development of human immunodeficiency virus type 1 (HIV-1) resistance. Monitoring for resistance will perhaps allow changes in therapy prior to deterioration in the patient's clinical or immunologic status. Our objective was to develop a rapid, specific...

journal_title：Journal of clinical microbiology

authors： Frenkel LM,Wagner LE 2nd,Atwood SM,Cummins TJ,Dewhurst S

更新日期：1995-02-01 00:00:00

abstract：:To evaluate the risk of a nosocomial spread of Pneumocystis carinii f. sp. hominis (P. carinii hominis), air filter samples from rooms of P. carinii pneumonia (PCP) patients, adjacent corridors, and other hospital environments have been investigated for the presence of P. carinii hominis. Amplified DNA from air filter...

journal_title：Journal of clinical microbiology

authors： Olsson M,Lidman C,Latouche S,Björkman A,Roux P,Linder E,Wahlgren M

更新日期：1998-06-01 00:00:00

abstract：:An accurate, rapid, and inexpensive method was developed for detecting and enumerating bacteria adherent to Foley urinary catheters based on malachite green staining of acridine orange-prestained specimens. This method has proven to be quick and reliable and will find application in quantitative studies of biomaterial...

journal_title：Journal of clinical microbiology

authors： Ladd TI,Schmiel D,Nickel JC,Costerton JW

更新日期：1985-06-01 00:00:00

abstract：:The fluorescent amplified-fragment length polymorphism (FAFLP) assay potentially amplifies a unique set of genome fragments from each bacterial clone. It uses stringently hybridizing primers which carry a fluorescent label. Precise fragment sizing is achieved by the inclusion of an internal size standard in every lane...

journal_title：Journal of clinical microbiology

authors： Arnold C,Metherell L,Willshaw G,Maggs A,Stanley J

更新日期：1999-05-01 00:00:00

abstract：:A 115-kDa exoantigen produced by Cryptococcus neoformans recognized by the previously described murine monoclonal antibody 7C9 has been purified from culture filtrate by a combination of membrane ultrafiltration, isoelectric focusing, and preparative gel electrophoresis. It is produced in late-log-phase cultures and i...

journal_title：Journal of clinical microbiology

authors： Hamilton AJ,Goodley J

更新日期：1993-02-01 00:00:00

abstract：:The development and application of a double-label immunofluorescence method which has the potential to screen for single or dual infections from any site, in single shell vial cultures, is described. In this study, a total of 1,141 ocular specimens were inoculated in shell vials, centrifuged at 15,000 X g for 1 h, inc...

journal_title：Journal of clinical microbiology

authors： Walpita P,Darougar S

更新日期：1989-07-01 00:00:00

abstract：:We compared the results of Gram staining and culture of cerebrospinal fluid to results obtained with a rapid PCR assay for the diagnosis of meningococcal meningitis in 281 cases of suspected bacterial meningitis. PCR had a sensitivity of 97% compared to a sensitivity of 55% for culture, and the PCR specificity was 99....

journal_title：Journal of clinical microbiology

authors： Richardson DC,Louie L,Louie M,Simor AE

更新日期：2003-08-01 00:00:00

abstract：:In the SIGNAL (Oxoid Ltd., Basingstoke, United Kingdom) blood culture system, gas produced by bacterial metabolism displaces medium from the culture bottle into an upper reservoir via a hollow needle. Displacement of media may provide a visual indication of the presence of both aerobic and anaerobic organisms in a sin...

journal_title：Journal of clinical microbiology

authors： Trombley C,Anderson JD

更新日期：1987-11-01 00:00:00