The Streptococcus milleri population of a cystic fibrosis clinic reveals patient specificity and intraspecies diversity.

abstract：:Actinobacillus actinomycetemcomitans is an important pathogen in the etiology of severe periodontitis. For epidemiological studies on the prevalence of certain pathogenic clones and transmission of this bacterium, adequate typing methods are necessary. The purpose of this study was to compare six different typing meth...

journal_title：Journal of clinical microbiology

authors： van Steenbergen TJ,Bosch-Tijhof CJ,van Winkelhoff AJ,Gmür R,de Graaff J

更新日期：1994-11-01 00:00:00

abstract：:The objective of this study was to measure the performance of the Affirm Ambient Temperature Transport System (ATTS) over time and to estimate the length of time the system can preserve a vaginal specimen containing the three common organisms causing vaginitis: Trichomonas vaginalis, Candida species, and Gardnerella v...

journal_title：Journal of clinical microbiology

authors： Brown HL,Fuller DA,Davis TE,Schwebke JR,Hillier SL

更新日期：2001-09-01 00:00:00

abstract：:Shortly after adopting a 6-week-old cat, a veterinarian was bitten on the left index finger. Within 3 weeks, he developed headache, fever, and left axillary lymphadenopathy. Initial blood cultures from the cat and veterinarian were sterile. Repeat cultures from the cat grew Bartonella-like organisms with lophotrichous...

journal_title：Journal of clinical microbiology

authors： Kordick DL,Hilyard EJ,Hadfield TL,Wilson KH,Steigerwalt AG,Brenner DJ,Breitschwerdt EB

更新日期：1997-07-01 00:00:00

abstract：:Potomac horse fever, a recently recognized disease of equines, characterized by high fever, leukopenia, and a profuse diarrhea, was studied for its etiology. An Ehrlichia organism was isolated in equine macrophage-fibroblast cell cultures and mouse macrophage cell cultures from the mononuclear cells of blood of infect...

journal_title：Journal of clinical microbiology

authors： Dutta SK,Myrup AC,Rice RM,Robl MG,Hammond RC

更新日期：1985-08-01 00:00:00

abstract：:Owing to the difficulties in isolating the virus and the lack of routine surveillance, the clinical significance of human parainfluenza virus 4 (HPIV-4) is less well defined than that of the other human parainfluenza viruses. We describe the first outbreak of HPIV-4 infection in a developmental disabilities unit, invo...

journal_title：Journal of clinical microbiology

authors： Lau SK,To WK,Tse PW,Chan AK,Woo PC,Tsoi HW,Leung AF,Li KS,Chan PK,Lim WW,Yung RW,Chan KH,Yuen KY

更新日期：2005-09-01 00:00:00

abstract：:Sera (920) were tested to evaluate the use of heated versus unheated sera in the hemagglutination treponemal test for syphilis. The heated and unheated samples were tested on the same day with the hemagglutination treponemal test for syphilis kit according to the manufacturer's protocol. Agreement of results between t...

journal_title：Journal of clinical microbiology

authors： Hambie EA,Larsen SA,Perryman MW,Pettit DE,Feeley JC

更新日期：1982-02-01 00:00:00

abstract：:The Centers for Disease Control and Prevention recommended confirming positive screening tests for Chlamydia trachomatis when positive predictive values are <90%. It is accepted that less sensitive tests (i.e., culture and immunoassays) should not be used to confirm the results of more sensitive nucleic acid amplifica...

journal_title：Journal of clinical microbiology

authors： Schachter J,Hook EW,Martin DH,Willis D,Fine P,Fuller D,Jordan J,Janda WM,Chernesky M

更新日期：2005-03-01 00:00:00

abstract：:Chronic hepatitis B virus (HBV) infection via perinatal transmission is common in the Asia-Pacific region, but related quasispecies (QS) characteristics are not yet defined. To investigate the homologous, full-length HBV QS after perinatal infection and their clinical implications, five pairs of mother-daughter patien...

journal_title：Journal of clinical microbiology

authors： Liu F,Yu DM,Huang SY,Yu JL,Zhang DH,Gong QM,Zhang XX

更新日期：2014-05-01 00:00:00

abstract：:The one-step single-tube betaine-free reverse transcription (RT)-loop-mediated isothermal amplification assay was developed for rapid diagnosis of hepatitis E virus. This assay amplified the target gene in less than 45 min (even as short as 20 min) under isothermal conditions at 63 degrees C, and the sensitivity of th...

journal_title：Journal of clinical microbiology

authors： Lan X,Yang B,Li BY,Yin XP,Li XR,Liu JX

更新日期：2009-07-01 00:00:00

abstract：:A prospective study was performed comparing the fluorescent antibody to membrane antigen (FAMA) test and the enzyme-linked immunosorbent assay (ELISA) for identifying susceptibility and seroconversion to varicella-zoster virus (VZV) infection. A total of 75 sera were collected from index cases and from sibling and par...

journal_title：Journal of clinical microbiology

authors： Iltis JP,Castellano GA,Gerber P,Le C,Vujcic LK,Quinnan GV Jr

更新日期：1982-11-01 00:00:00

abstract：:Despite its theoretical advantages, direct antimicrobial susceptibility testing (DST) of urine specimens remains controversial largely because of concerns regarding its accuracy, particularly with mixed cultures. To evaluate the performance of DST in the setting of acute urinary tract infection (UTI), we performed DST...

journal_title：Journal of clinical microbiology

authors： Johnson JR,Tiu FS,Stamm WE

更新日期：1995-09-01 00:00:00

abstract：:Strains of Mycobacterium avium, Mycobacterium intracellulare, Mycobacterium scrofulaceum, Mycobacterium xenopi, and Mycobacterium gordonae were identified by high-performance liquid chromatography (HPLC) analysis of mycolic acids as bromophenacyl esters. HPLC criteria were used to develop a flow chart identification s...

journal_title：Journal of clinical microbiology

authors： Butler WR,Thibert L,Kilburn JO

更新日期：1992-10-01 00:00:00

abstract：:Quantitative lactate determinations were performed on cerebrospinal fluids to assess their value in the rapid diagnosis of bacterial and mycotic meningitis and to evaluate their value in assessing the prognosis in these patients. Cerebrospinal fluid lactate concentrations were elevated in all patients with untreated b...

journal_title：Journal of clinical microbiology

authors： Komorowski RA,Farmer SG,Hanson GA,Hause LL

更新日期：1978-07-01 00:00:00

abstract：:A direct comparison of two automated blood culture systems was conducted to compare their ability to detect Candida growth. The systems evaluated were the BACTEC 9240 (Bactec) and BacT/ALERT 3D (BacT). The aerobic, anaerobic, and mycology media for each system were evaluated: Bactec Plus Aerobic/F, Plus Anaerobic/F, a...

journal_title：Journal of clinical microbiology

authors： Horvath LL,George BJ,Murray CK,Harrison LS,Hospenthal DR

更新日期：2004-01-01 00:00:00

abstract：:An immunofluorescent-antibody test was developed for rapid detection of Pseudomonas aeruginosa in blood cultures. The test uses a murine monoclonal antibody specific for all strains of P. aeruginosa. In initial tests, bright uniform immunofluorescence signals were seen when each of the 17 international serotypes, as w...

journal_title：Journal of clinical microbiology

authors： Counts GW,Schwartz RW,Ulness BK,Hamilton DJ,Rosok MJ,Cunningham MD,Tam MR,Darveau RP

更新日期：1988-06-01 00:00:00

abstract：:Salmonella enterica serovar Senftenberg is a common nontyphoidal Salmonella serotype which causes human Salmonella infections worldwide. In this study, 182 S. Senftenberg isolates, including 17 atypical non-hydrogen sulfide (H2S)-producing isolates, were detected in China from 2005 to 2011. The microbiological and gen...

journal_title：Journal of clinical microbiology

authors： Yi S,Xie J,Liu N,Li P,Xu X,Li H,Sun J,Wang J,Liang B,Yang C,Wang X,Hao R,Wang L,Wu Z,Zhang J,Wang Y,Huang L,Sun Y,Klena JD,Meng J,Qiu S,Song H

更新日期：2014-07-01 00:00:00

abstract：:About 2.5 years after the first isolation of the VanA phenotype of vancomycin-resistant Enterococcus faecium (VREM) in Poland, the first VREM strains with the VanB phenotype have emerged independently in two different Warsaw hospitals. In one of these the VREM strain was selected during the long-term antimicrobial tre...

journal_title：Journal of clinical microbiology

authors： Kawalec M,Gniadkowski M,Zielińska U,Kłos W,Hryniewicz W

更新日期：2001-02-01 00:00:00

abstract：:We have isolated one sorbitol-nonfermenting (SNF) Escherichia coli O157:H7 isolate and five sorbitol-fermenting (SF) E. coli O157:H(-) isolates that do not contain Shiga toxin (Stx) genes (stx). Isolates originated from patients with diarrhea (n = 4) and hemolytic-uremic syndrome (HUS) (n = 2). All isolates harbored a...

journal_title：Journal of clinical microbiology

authors： Schmidt H,Scheef J,Huppertz HI,Frosch M,Karch H

更新日期：1999-11-01 00:00:00

abstract：:Noroviruses are the leading cause of outbreaks of gastroenteritis in the world. At present, norovirus genogroup II, genotype 4 (GII/4), strains are the most prevalent in many countries. In this study we investigated 55 outbreaks and 35 sporadic cases of norovirus-associated gastroenteritis in food handlers in food-cat...

journal_title：Journal of clinical microbiology

authors： Ozawa K,Oka T,Takeda N,Hansman GS

更新日期：2007-12-01 00:00:00

abstract：:We evaluated a commercially available second-generation anti-H. pylori immunoglobulin G enzyme immunoassay (EIA) (Cobas Core Anti-Helicobacter pylori EIA; Roche S. A., Basel, Switzerland) for serodiagnosis of H. pylori infection. The results of the assay were assessed in relation to the results of bacterial culture, u...

journal_title：Journal of clinical microbiology

authors： Goossens H,Glupczynski Y,Burette A,Van den Borre C,Butzler JP

更新日期：1992-01-01 00:00:00

abstract：:Mueller-Hinton (MH) agar media from various commercial sources, either supplemented or not supplemented with 5% sheep blood, were studied to determine their effect on disk diffusion susceptibility testing results obtained with 90 strains of group D streptococci and four cephalosporins. The cephalosporins investigated ...

journal_title：Journal of clinical microbiology

authors： Sahm DF,Baker CN,Jones RN,Thornsberry C

更新日期：1983-10-01 00:00:00

abstract：:Whole chromosomal DNA probes were used to identify clinical isolates of Mycobacterium tuberculosis, Mycobacterium avium complex, and Mycobacterium gordonae. The probe for M. tuberculosis was prepared from Mycobacterium bovis BCG, which has been shown to be closely related to M. tuberculosis. A probe for the M. avium c...

journal_title：Journal of clinical microbiology

authors： Roberts MC,McMillan C,Coyle MB

更新日期：1987-07-01 00:00:00

abstract：:A direct and an indirect enzyme immunoassay (EIA) were evaluated against a standard of electron microscopy after direct ultracentrifugation of the specimen for their performances in detecting rotaviruses. The indirect EIA had variable background activity which influenced test specificity. The indirect EIA control (tes...

journal_title：Journal of clinical microbiology

authors： Hammond GW,Ahluwalia GS,Barker FG,Horsman G,Hazelton PR

更新日期：1982-07-01 00:00:00

abstract：:Fast and accurate identification and typing of pathogens are essential for effective surveillance and outbreak detection. The current routine procedure is based on a variety of techniques, making the procedure laborious, time-consuming, and expensive. With whole-genome sequencing (WGS) becoming cheaper, it has huge po...

journal_title：Journal of clinical microbiology

authors： Joensen KG,Scheutz F,Lund O,Hasman H,Kaas RS,Nielsen EM,Aarestrup FM

更新日期：2014-05-01 00:00:00

abstract：:A total of 101 isolates of penicillinase-producing and non-penicillinase-producing Neisseria gonorrhoeae with known nutritional requirements, plasmid content, and serovars, were examined for lectin agglutination patterns. These isolates were from outbreaks in Georgia, California, Hawaii, and Pennsylvania. Cell suspens...

journal_title：Journal of clinical microbiology

authors： Schalla WO,Whittington WL,Rice RJ,Larsen SA

更新日期：1985-09-01 00:00:00

abstract：:Serratia plymuthica was isolated from the blood and from a central venous catheter tip in a 54-year-old man with alcoholic cirrhosis and clinical signs of sepsis. This was the seventh reported isolate of S. plymuthica from a clinical specimen and the first in which the organism was clearly a significant pathogen. ...

journal_title：Journal of clinical microbiology

authors： Horowitz HW,Nadelman RB,Van Horn KG,Weekes SE,Goyburu L,Wormser GP

更新日期：1987-08-01 00:00:00

abstract：:A toxic shock syndrome toxin 1 (TSST-1) antibody-binding protein produced by an ovine-associated strain of Staphylococcus aureus was examined. The protein showed total identity to TSST-1 by immunodiffusion analysis. Western blots (immunoblots) of proteins separated by isoelectric focusing revealed that the TSST-1 anti...

journal_title：Journal of clinical microbiology

authors： Ho G,Campbell WH,Carlson E

更新日期：1989-01-01 00:00:00

abstract：:The prevalence of the Epstein-Barr virus (EBV) Taiwan variant was investigated in the throat washing (TW) samples from patients with head and neck tumors, persons with nonmalignant diseases, and healthy adults in Taiwan. By using the EBV (BNLF-1 gene)-specific primers and PCR, the EBV latent membrane protein gene BNLF...

journal_title：Journal of clinical microbiology

authors： Jeng KC,Hsu CY,Liu MT,Chung TT,Liu ST

更新日期：1994-01-01 00:00:00

abstract：:Vaginal swab specimens may be preferable to cervical swab or urine specimens for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae because of the ease of specimen collection and transport. The purpose of this study was to evaluate whether vaginal swab specimens are equivalent to cervical swab specimens ...

journal_title：Journal of clinical microbiology

authors： Cosentino LA,Landers DV,Hillier SL

更新日期：2003-08-01 00:00:00

abstract：:Recent studies have shown the public health importance of identifying individuals with acute human immunodeficiency virus infection (AHI); however, the cost of nucleic acid amplification testing (NAAT) makes individual testing of at-risk individuals prohibitively expensive in many settings. Pooled NAAT (or group testi...

journal_title：Journal of clinical microbiology

authors： Westreich DJ,Hudgens MG,Fiscus SA,Pilcher CD

更新日期：2008-05-01 00:00:00