Detection of sialidase (neuraminidase) activity in Actinomyces species by using 2'-(4-methylumbelliferyl)alpha-D-N-acetylneuraminic acid in a filter paper spot test.

abstract：:Polyomavirus BK (BKV) establishes latent infection in various human tissues, including the kidney. Reactivation following renal transplantation (RT) may cause BKV-associated nephropathy, leading to graft loss. BKV reactivation is often associated with extensive rearrangements in the BKV noncoding regulatory region (NC...

journal_title：Journal of clinical microbiology

authors： Perets TT,Silberstein I,Rubinov J,Sarid R,Mendelson E,Shulman LM

更新日期：2009-05-01 00:00:00

abstract：:The Lyme disease-associated spirochete, Borrelia burgdorferi, is maintained in enzootic cycles involving Ixodes ticks and small mammals. Previous studies demonstrated that B. burgdorferi expresses outer surface protein A (OspA) but not OspC when residing in the midgut of unfed ticks. However, after ticks feed on blood...

journal_title：Journal of clinical microbiology

authors： Schwan TG,Piesman J

更新日期：2000-01-01 00:00:00

abstract：:Nucleic acid amplification tests, such as PCR, are the method of choice for respiratory virus testing, due to their superior diagnostic accuracy and fast turnaround time. The Panther Fusion (Fusion; Hologic) system has an array of highly sensitive in vitro diagnostic (IVD) real-time PCR assays for respiratory viruses,...

journal_title：Journal of clinical microbiology

authors： Stellrecht KA,Cimino JL,Maceira VP

更新日期：2020-05-26 00:00:00

abstract：:A novel PCR detection assay that amplifies the Helicobacter pylori-specific vacuolating cytotoxin gene (vacA) and thus enables rapid diagnosis of infection is described. Additionally, a real-time probe hybridization melting point analysis assay to detect all three mutations in the 23S rRNA gene associated with clarith...

journal_title：Journal of clinical microbiology

authors： Chisholm SA,Owen RJ,Teare EL,Saverymuttu S

更新日期：2001-04-01 00:00:00

abstract：:Myxozoan spores were detected in fecal samples from three patients presenting with abdominal pain and/or diarrhea. The spores were identical to those of Myxobolus plectroplites, a previously described pathogen from the freshwater fish Plectroplites ambiguus. All patients had recently eaten fish caught from local water...

journal_title：Journal of clinical microbiology

authors： Boreham RE,Hendrick S,O'Donoghue PJ,Stenzel DJ

更新日期：1998-12-01 00:00:00

abstract：:Broad-spectrum analysis for pathogens in patients with respiratory tract infections is becoming more relevant as the number of potential infectious agents is still increasing. Here we describe the new multiparameter RespiFinder assay, which is based on the multiplex ligation-dependent probe amplification (MLPA) techno...

journal_title：Journal of clinical microbiology

authors： Reijans M,Dingemans G,Klaassen CH,Meis JF,Keijdener J,Mulders B,Eadie K,van Leeuwen W,van Belkum A,Horrevorts AM,Simons G

更新日期：2008-04-01 00:00:00

abstract：:The diagnosis of brucellosis in cattle and small ruminants requires the use of more than one serological test. The complement fixation test (CFT), the rose bengal test (RBT), and the serum agglutination test (SAT) are among the most useful tests for routine diagnosis. The microagglutination test (MAT) was developed as...

journal_title：Journal of clinical microbiology

authors： Baum M,Zamir O,Bergman-Rios R,Katz E,Beider Z,Cohen A,Banai M

更新日期：1995-08-01 00:00:00

abstract：:We describe a simple method for the rapid detection of bovine viral diarrhea virus (BVDV) that uses a one-tube reverse transcription PCR (RT-PCR) and total RNA extracted directly from a variety of bovine specimens, including whole blood and tissues. Reagents for both RT and PCR were combined in a one-tube, single-buff...

journal_title：Journal of clinical microbiology

authors： Hamel AL,Wasylyshen MD,Nayar GP

更新日期：1995-02-01 00:00:00

abstract：:A commercial DNA probe kit designed to detect rRNA from legionellae was evaluated for its ability to correctly discriminate between legionellae and non-legionellae taken from culture plates. The probe kit, made by the Gen-Probe Corp. (San Diego, Calif.), was radiolabeled with 125I, and probe bacterial RNA hybridizatio...

journal_title：Journal of clinical microbiology

authors： Edelstein PH

更新日期：1986-03-01 00:00:00

abstract：:A case of tick-borne encephalitis (TBE) has not been reported for many years in Japan, although a serological survey of sera from domestic animals suggested the presence of TBE foci in Hokkaido, the northern island of Japan. Studies were conducted to prove the presence of an endemic focus of TBE virus in Japan by mean...

journal_title：Journal of clinical microbiology

authors： Takashima I,Morita K,Chiba M,Hayasaka D,Sato T,Takezawa C,Igarashi A,Kariwa H,Yoshimatsu K,Arikawa J,Hashimoto N

更新日期：1997-08-01 00:00:00

abstract：:The development of diagnostic susceptibility tests for CEM-101, a new fluoroketolide, was addressed by structured studies to determine the optimal disk diffusion test concentration and effects of various testing conditions or supplements and to establish the quality control (QC) ranges for reference broth microdilutio...

journal_title：Journal of clinical microbiology

authors： Jones RN,Ross JE,Rhomberg PR,Quality Control Working Group.

更新日期：2010-04-01 00:00:00

abstract：:Strains of Mycobacterium avium, Mycobacterium intracellulare, Mycobacterium scrofulaceum, Mycobacterium xenopi, and Mycobacterium gordonae were identified by high-performance liquid chromatography (HPLC) analysis of mycolic acids as bromophenacyl esters. HPLC criteria were used to develop a flow chart identification s...

journal_title：Journal of clinical microbiology

authors： Butler WR,Thibert L,Kilburn JO

更新日期：1992-10-01 00:00:00

abstract：:The binding of human immunoglobulin G by Fc receptors in herpes simplex virus (HSV)-infected cells can cause false-positive interpretations in the immunofluorescence test for HSV antibody. When the infected cell smears were treated with 10% glacial acetic acid for 5 min and rinsed in phosphate-buffered saline before t...

journal_title：Journal of clinical microbiology

authors： Gallo D

更新日期：1986-10-01 00:00:00

abstract：:The food-borne pathogen Listeria monocytogenes is genetically heterogeneous. Although some clonal groups have been implicated in multiple outbreaks, there is currently no consensus on how "epidemic clones" should be defined. The objectives of this work were to compare the patterns of sequence diversity on two sets of ...

journal_title：Journal of clinical microbiology

authors： Cantinelli T,Chenal-Francisque V,Diancourt L,Frezal L,Leclercq A,Wirth T,Lecuit M,Brisse S

更新日期：2013-11-01 00:00:00

abstract：:A total of 145 clinical strains of Candida species were tested by the Clinical and Laboratory Standards Institute M27-A3 methodology to determine if replacing water with dimethyl sulfoxide as the solvent for fluconazole and flucytosine impacted the in vitro potency. No significant differences in MIC values were observ...

journal_title：Journal of clinical microbiology

authors： Fothergill AW,Sanders C,Wiederhold NP

更新日期：2013-06-01 00:00:00

abstract：:An agglutination test for immunoglobulin G (IgG) Toxoplasma antibodies with acetone-fixed tachyzoites (AC antigens; AC agglutination test) was positive only with sera from patients during the acute stage of their infection. In contrast, when the test was performed with Formalin-fixed tachyzoites (HS antigens; HS agglu...

journal_title：Journal of clinical microbiology

authors： Suzuki Y,Thulliez P,Desmonts G,Remington JS

更新日期：1988-05-01 00:00:00

abstract：:We evaluated two commercial F1 antigen capture-based immunochromatographic dipsticks, Yersinia Pestis (F1) Smart II and Plague BioThreat Alert test strips, in detecting plague bacilli by using whole-blood samples from mice experimentally infected with Yersinia pestis CO92. To assess the specificities of these dipstick...

journal_title：Journal of clinical microbiology

authors： Sha J,Endsley JJ,Kirtley ML,Foltz SM,Huante MB,Erova TE,Kozlova EV,Popov VL,Yeager LA,Zudina IV,Motin VL,Peterson JW,DeBord KL,Chopra AK

更新日期：2011-05-01 00:00:00

abstract：:Species of the genus Bartonella are currently recognized in growing numbers and are involved in an increasing variety of human diseases, mainly trench fever, Carrion's disease, bacillary angiomatosis, endocarditis, cat scratch disease, neuroretinitis, and asymptomatic bacteremia. Such a wide spectrum of infections mak...

journal_title：Journal of clinical microbiology

authors： Houpikian P,Raoult D

更新日期：2001-08-01 00:00:00

abstract：:The detection of amplified products resulting from polymerase chain reactions (PCRs) remains a complicated process. To simplify the detection procedures, we developed a colorimetric microtiter plate hybridization assay for the specific detection of 5'-biotinylated PCR fragments of Mycobacterium leprae DNA. For this as...

journal_title：Journal of clinical microbiology

authors： van der Vliet GM,Hermans CJ,Klatser PR

更新日期：1993-03-01 00:00:00

abstract：:Nosocomial vancomycin-resistant Enterococcus (VRE) infections have been described in only small numbers of pediatric patients. In none of these studies were multivariate analyses performed to assess which factors were independent risk factors in these patients. In the present cohort study of patients admitted to our h...

journal_title：Journal of clinical microbiology

authors： Singh-Naz N,Sleemi A,Pikis A,Patel KM,Campos JM

更新日期：1999-02-01 00:00:00

abstract：:This study involved the rapid, direct identification of Staphylococcus aureus, Streptococcus pneumoniae, Escherichia coli, Haemophilus influenzae, Enterococcus sp., and Streptococcus agalactiae from positive blood culture bottles (BACTEC, Johnston Laboratories, Inc.) by using the AccuProbe (Gen-Probe, San Diego, Calif...

journal_title：Journal of clinical microbiology

authors： Davis TE,Fuller DD

更新日期：1991-10-01 00:00:00

abstract：:We evaluated the diagnostic value of antibody to Blastomyces dermatitidis A precipitin arcs for identifying B. dermatitidis cultures by the exoantigen technique with 88 isolates from the United States and Canada, 12 from Africa, and 1 each from India and Israel. In addition, we studied 190 mycelial-form isolates of ot...

journal_title：Journal of clinical microbiology

authors： Kaufman L,Standard PG,Weeks RJ,Padhye AA

更新日期：1983-07-01 00:00:00

abstract：:A microwave-accelerated direct immunofluorescence staining method which requires only 20 min from specimen receipt to interpretation is as effective as conventional methods for detecting respiratory syncytial virus and influenza A virus antigens in clinical specimens. The time required compares favorably with that for...

journal_title：Journal of clinical microbiology

authors： Hite SA,Huang YT

更新日期：1996-07-01 00:00:00

abstract：:A procedure for detecting pneumococcal capsular polysaccharides in immune complexes is described. Separation of antigen from immune complexes is achieved by electrophoresis at 56 degrees C. ...

journal_title：Journal of clinical microbiology

authors： Coonrod JD,Leach RP

更新日期：1978-08-01 00:00:00

abstract：:Trichosporon species are relevant etiologic agents of hospital-acquired infections. High mortality rates are attributed to Trichosporon deep-seated infections in immunocompromised individuals, making fast and accurate species identification relevant for hastening the discovery of best-targeted therapy. Recently, Trich...

journal_title：Journal of clinical microbiology

authors： de Almeida JN Jr,Favero Gimenes VM,Francisco EC,Machado Siqueira LP,Gonçalves de Almeida RK,Guitard J,Hennequin C,Colombo AL,Benard G,Rossi F

更新日期：2017-08-01 00:00:00

abstract：:It has been shown that some, but not all, Escherichia coli strains isolated from urine adhere, in vitro, to the surface of uroepithelial or vaginal cells. In the present study, 212 strains, isolated from urine of 212 infected patients, were tested for adhesion by using an in vitro human cell line assay. A variable deg...

journal_title：Journal of clinical microbiology

authors： Chabanon G,Hartley CL,Richmond MH

更新日期：1979-10-01 00:00:00

abstract：:In this study, the optimal combination of three commercial glycoprotein G-2 (gG-2)-based herpes simplex virus type 2 (HSV-2) type-specific enzyme-linked immunosorbent assays (Euroimmun anti-HSV-2 immunoglobulin G [IgG] ELISA [Eu2], Gull HSV-2-specific IgG ELISA [Gu2], and Radim HSV-2 IgG ELISA [Ra2]) and one gG-2-base...

journal_title：Journal of clinical microbiology

authors： Eing BR,Lippelt L,Lorentzen EU,Hafezi W,Schlumberger W,Steinhagen K,Kühn JE

更新日期：2002-02-01 00:00:00

abstract：:A new principle that uses anti-immunoglobulin M-coated polystyrene microtiter plates for the detection of immunoglobulin M antibodies against Toxoplasma gondii by an enzyme-linked immunosorbent assay was developed. ...

journal_title：Journal of clinical microbiology

authors： Duermeyer W,Wielaard F,van Gruijthuijsen H,Swinkels J

更新日期：1980-12-01 00:00:00

abstract：:Seven commercial rotavirus antigen assays were compared with in-house PCR methods for detecting rotavirus in stool specimens. The assay sensitivities were 80% to 100%, while the specificities were 54.3% for one commercial immunochromatographic (ICT) method and 99.4% to 100% for other assays. Thus, except for one comme...

journal_title：Journal of clinical microbiology

authors： Ye S,Lambert SB,Grimwood K,Roczo-Farkas S,Nimmo GR,Sloots TP,Kirkwood CD,Whiley DM

更新日期：2015-01-01 00:00:00

abstract：:Aberrant filamentous forms of Escherichia coli were observed on direct Gram stain of blood cultures from a patient being treated with the beta-lactam antibiotic cephalexin. After the institution of an alternative antibiotic regimen which included a different cell wall-active agent, E. coli of normal morphology was det...

journal_title：Journal of clinical microbiology

authors： Magnussen CR,Hruska JF

更新日期：1980-11-01 00:00:00