RespiFinder: a new multiparameter test to differentially identify fifteen respiratory viruses.

abstract：:Lipoglycans from mycoplasmas possess the ability to induce the elaboration of procoagulant activity. This represents a third endotoxic property for these polymers, in addition to the pyrogenicity and clotting of Limulus lysate reported previously. Lipoglycans from three species of Acholeplasma (A. axanthum, A. granula...

journal_title：Journal of clinical microbiology

authors： Miragliotta G,Barone G,Monno RA,Fumarola D,Smith PF

更新日期：1987-07-01 00:00:00

abstract：:An outbreak of folliculitis caused by Pseudomonas aeruginosa serotype O:7 occurred among the guests of a hotel in St. John's, Newfoundland, Canada, and the source of the infection was traced to the hotel whirlpool. Of 36 persons who used the whirlpool, 26 (72%) developed folliculitis within 1 to 5 days after exposure;...

journal_title：Journal of clinical microbiology

authors： Ratnam S,Hogan K,March SB,Butler RW

更新日期：1986-03-01 00:00:00

abstract：:Prosthetic joint infection (PJI) is a rare but refractory complication of arthroplasty. Accurate identification of pathogens is a key step for successful treatment of PJI, which remains a challenge for clinicians and laboratory workers. We designed a combined culture method with sonication of implants and incubation i...

journal_title：Journal of clinical microbiology

authors： Shen H,Tang J,Wang Q,Jiang Y,Zhang X

更新日期：2015-03-01 00:00:00

abstract：:An immunofluorescent-antibody test was developed for rapid detection of Pseudomonas aeruginosa in blood cultures. The test uses a murine monoclonal antibody specific for all strains of P. aeruginosa. In initial tests, bright uniform immunofluorescence signals were seen when each of the 17 international serotypes, as w...

journal_title：Journal of clinical microbiology

authors： Counts GW,Schwartz RW,Ulness BK,Hamilton DJ,Rosok MJ,Cunningham MD,Tam MR,Darveau RP

更新日期：1988-06-01 00:00:00

abstract：:Salmonella enteritidis, serotype Horsham, identified rarely worldwide, was isolated from the stools of six American Indians with gastroenteritis. The patients were from separate tribes located in three geographically distinct villages. Although significant contact was demonstrated between ill individuals within two tr...

journal_title：Journal of clinical microbiology

authors： Castle SP,Nims LJ,Lapham SC

更新日期：1983-07-01 00:00:00

abstract：:A trunk of human cytidylate-phosphate-deoxyguanylate-binding protein/CXXC finger protein 1 (CFP1), immobilized onto an aminohexyl-Sepharose column, can be used as a preanalytical tool for the selective enrichment of bacterial DNA from mixed solutions with high amounts of human background DNA for nucleic acid amplifica...

journal_title：Journal of clinical microbiology

authors： Sachse S,Straube E,Lehmann M,Bauer M,Russwurm S,Schmidt KH

更新日期：2009-04-01 00:00:00

abstract：:Four independent molecular methods were used to characterize the Salmonella enterica subsp. enterica serovar choleraesuis live vaccine strains SC-54 and Suisaloral and to differentiate them from S. choleraesuis field isolates. Plasmid analysis revealed the presence of seven plasmid profiles. A virulence plasmid of 52-...

journal_title：Journal of clinical microbiology

authors： Weide-Botjes M,Liebisch B,Schwarz S,Watts JL

更新日期：1996-10-01 00:00:00

abstract：:Human infections with Salmonella enterica subspecies enterica serovar Senftenberg are often associated with exposure to poultry flocks, farm environments, or contaminated food. The recent emergence of multidrug-resistant isolates has raised public health concerns. In this study, comparative genomics and phenotypic ana...

journal_title：Journal of clinical microbiology

authors： Abd El Ghany M,Shi X,Li Y,Ansari HR,Hill-Cawthorne GA,Ho YS,Naeem R,Pickard D,Klena JD,Xu X,Pain A,Hu Q

更新日期：2016-08-01 00:00:00

abstract：:HIV-1 drug resistance (HIVDR) assays are important tools in clinical management of HIV-infected patients on antiretroviral therapy (ART) and surveillance of drug-resistant variants at population levels. The high cost associated with commercial assays hinders their use in resource-limited settings. We adopted and valid...

journal_title：Journal of clinical microbiology

authors： Inzaule S,Yang C,Kasembeli A,Nafisa L,Okonji J,Oyaro B,Lando R,Mills LA,Laserson K,Thomas T,Nkengasong J,Zeh C

更新日期：2013-02-01 00:00:00

abstract：:We report the molecular epidemiology of 27 clinical multidrug-resistant Staphylococcus epidermidis (MDRSE) isolates collected between 2003 and 2007 in an Australian teaching hospital. The dominant genotype (sequence type 2 [ST2]) accounted for 85% of the isolates tested and was indistinguishable from an MDRSE genotype...

journal_title：Journal of clinical microbiology

authors： Widerström M,McCullough CA,Coombs GW,Monsen T,Christiansen KJ

更新日期：2012-06-01 00:00:00

abstract：:A previous study suggested that delayed sample preparation can cause a false-positive plasma cytomegalovirus (CMV) PCR. We evaluated 78 samples submitted for CMV testing, and we report that a 24-h delay in plasma separation did not affect viral quantitation by real-time PCR. Laboratories should evaluate specimen handl...

journal_title：Journal of clinical microbiology

authors： Nesbitt SE,Cook L,Jerome KR

更新日期：2004-03-01 00:00:00

abstract：:During February and March 1984, 207 fecal samples from infants and children with gastroenteritis were tested for rotavirus with four techniques: two enzyme immunoassays (Rotazyme; Abbott Laboratories, North Chicago, Ill., and Enzygnost-Rotavirus; Calbiochem-Behring, La Jolla, Calif.) and two latex agglutination tests ...

journal_title：Journal of clinical microbiology

authors： Sambourg M,Goudeau A,Courant C,Pinon G,Denis F

更新日期：1985-04-01 00:00:00

abstract：:A multiplex PCR (mPCR) protocol was developed for simultaneous detection of the gyrB gene in Streptococcus pneumoniae, Streptococcus mitis, and Streptococcus oralis, and the specificity was evaluated using 141 coccus strains. Genomic DNAs purified from S. pneumoniae, S. mitis, and S. oralis strains were efficiently de...

journal_title：Journal of clinical microbiology

authors： Kim W,Park HK,Hwang WJ,Shin HS

更新日期：2013-03-01 00:00:00

abstract：:The recent emergence of the multidrug-resistant and pathogenic yeast Candida auris continues to cause public health concern worldwide. C. auris is alarming because it causes health care-associated outbreaks and can establish invasive infections with high mortality rates. Transmission between patients is facilitated by...

journal_title：Journal of clinical microbiology

authors： Sexton DJ,Kordalewska M,Bentz ML,Welsh RM,Perlin DS,Litvintseva AP

更新日期：2018-11-27 00:00:00

abstract：:We studied the use of dried spots of bodily fluids (plasma, whole blood, and mother's milk) on filter paper as a means of sample collection and storage for human immunodeficiency virus type 1 (HIV-1) viral load testing under stringent field conditions. Plasma placed directly in lysis buffer, which is customarily used ...

journal_title：Journal of clinical microbiology

authors： Ayele W,Schuurman R,Messele T,Dorigo-Zetsma W,Mengistu Y,Goudsmit J,Paxton WA,de Baar MP,Pollakis G

更新日期：2007-03-01 00:00:00

abstract：:We investigated a method to reduce oxygen tension and produce anaerobic conditions conveniently by coculture with a nonfermentative organism. The biochemical reactions of 94 strains representing 41 different species were determined using prereduced media and gas chromatography according to standard methods. The standa...

journal_title：Journal of clinical microbiology

authors： Hussain Z,Bürger H,Lannigan R,Groves DJ

更新日期：1984-02-01 00:00:00

abstract：:We describe two cases of eumycetoma in the legs. The infections could not be adequately diagnosed by classical mycology, but the causative agents were successfully identified as Madurella mycetomatis by species-specific PCR and DNA sequencing. ...

journal_title：Journal of clinical microbiology

authors： Ahmed AO,Desplaces N,Leonard P,Goldstein F,De Hoog S,Verbrugh H,van Belkum A

更新日期：2003-12-01 00:00:00

abstract：:Common causes of chronic diarrhea among travelers worldwide include protozoan parasites. The majority of parasitic infections are caused by Giardia duodenalis, Entamoeba histolytica, Cryptosporidium parvum, and Cryptosporidium hominis Similarly, these species cause the majority of parasitic diarrhea acquired in the Un...

journal_title：Journal of clinical microbiology

authors： Madison-Antenucci S,Relich RF,Doyle L,Espina N,Fuller D,Karchmer T,Lainesse A,Mortensen JE,Pancholi P,Veros W,Harrington SM

更新日期：2016-11-01 00:00:00

abstract：:Broth microdilution and disk diffusion susceptibility testing were performed on 73 strains of Pasteurella species isolated from human infections and on five American Type Culture Collection strains of Pasteurella species. Both methods appear reliable for testing susceptibilities of Pasteurella species. ...

journal_title：Journal of clinical microbiology

authors： Citron DM,Warren YA,Fernandez HT,Goldstein MA,Tyrrell KL,Goldstein EJ

更新日期：2005-05-01 00:00:00

abstract：:Several methods have been previously suggested for the presumptive identification of Gardnerella vaginalis in clinical laboratories, but none is entirely satisfactory. We previously found that sodium polyanetholesulfonate (SPS) inhibits G. vaginalis in blood culture media. We compared susceptibility to an SPS-containi...

journal_title：Journal of clinical microbiology

authors： Reimer LG,Reller LB

更新日期：1985-02-01 00:00:00

abstract：:A new latex agglutination test (Pastorex Staph-Plus, Sanofi Diagnostics Pasteur), consisting of a mixture of latex particles coated with fibrinogen and immunoglobulin G for the detection of clumping factor and protein A and latex particles sensitized with monoclonal antibodies directed to Staphylococcus aureus serotyp...

journal_title：Journal of clinical microbiology

authors： Fournier JM,Bouvet A,Mathieu D,Nato F,Boutonnier A,Gerbal R,Brunengo P,Saulnier C,Sagot N,Slizewicz B

更新日期：1993-05-01 00:00:00

abstract：:Actinomyces viscosus is a gram-positive, non-acid-fact, facultative, catalase-positive, filamentous, or diphtheroidal microorganism. It was isolated from six canine infections during a period of 1.5 years. The organism was cultured from exudate and flaky granules aspirated from infectious granulomas and empyemas. All ...

journal_title：Journal of clinical microbiology

authors： Davenport AA,Carter GR,Patterson MJ

更新日期：1975-01-01 00:00:00

abstract：:The PathoTec Rapid I-D System for identifying Enterobacteriaceae was evaluated with 471 cultures. In 4,910 individual test comparisons, 95.5% of the results agreed, with results of only two test strips, those for esculin hydrolysis and urease production, agreeing with conventional tests in less than 94% of the trials....

journal_title：Journal of clinical microbiology

authors： Smith PB,Rhoden DL,Tomfohrde KM

更新日期：1975-04-01 00:00:00

abstract：:D-Arabinitol (DA) is a useful diagnostic marker for candidiasis in patients with neutropenia and other high-risk groups, but its use in unselected patients with a broad range of underlying diseases and conditions has not been studied. We used an automated enzymatic fluorometric assay to measure serum DA/creatinine rat...

journal_title：Journal of clinical microbiology

authors： Yeo SF,Huie S,Sofair AN,Campbell S,Durante A,Wong B

更新日期：2006-11-01 00:00:00

abstract：:Six separate human immunodeficiency virus-positive patients with cryptococcal meningitis were each found to have been infected with a unique strain of Cryptococcus neoformans on the basis of genomic DNA finger-printing analysis with the microsatellite sequence-containing oligonucleotide probe (GGAT)4 and by random amp...

journal_title：Journal of clinical microbiology

authors： Sullivan D,Haynes K,Moran G,Shanley D,Coleman D

更新日期：1996-07-01 00:00:00

abstract：:Traditional ribotyping detects genomic restriction fragment length polymorphisms by probing chromosomal DNA with rRNA. Although it is a powerful method for determining the molecular epidemiology of bacterial pathogens, technical difficulties limit its application. As an alternative, polymorphisms were sought in the 16...

journal_title：Journal of clinical microbiology

authors： Kostman JR,Edlind TD,LiPuma JJ,Stull TL

更新日期：1992-08-01 00:00:00

abstract：:We compared the results obtained with six commercial MIC test systems (Etest, MicroScan, Phoenix, Sensititre, Vitek Legacy, and Vitek 2 systems) and three reference methods (agar dilution, disk diffusion, and vancomycin [VA] agar screen [VScr]) with the results obtained by the Clinical and Laboratory Standards Institu...

journal_title：Journal of clinical microbiology

authors： Swenson JM,Anderson KF,Lonsway DR,Thompson A,McAllister SK,Limbago BM,Carey RB,Tenover FC,Patel JB

更新日期：2009-07-01 00:00:00

abstract：:We have previously demonstrated homology between a 181-bp fragment of IS6110 and DNA from mycobacteria other than tuberculosis-causing mycobacteria (MOTT). Genomic DNA from 14 strains of MOTT was digested with PvuII and was hybridized with a probe derived from the 181-bp fragment and the INS1/INS2 international standa...

journal_title：Journal of clinical microbiology

authors： McHugh TD,Newport LE,Gillespie SH

更新日期：1997-07-01 00:00:00

abstract：:Antifungal susceptibility testing of pathogenic molds is being developed. A simple screening semisolid agar antifungal susceptibility (SAAS) test accurately measures susceptibilities of yeasts. The performance of the SAAS screening test for filamentous fungi was assessed by comparing MICs of four antifungals (amphoter...

journal_title：Journal of clinical microbiology

authors： Kuzucu C,Rapino B,McDermott L,Hadley S

更新日期：2004-03-01 00:00:00

abstract：:Formalin-ethyl ether sedimentation, Formalin-ethyl acetate sedimentation, and zinc sulfate flotation techniques were compared using over 250 clinical parasitology specimens. Fifty positive specimens were identified, and a variety of parasites, including amoebae, flagellates, cestodes, nematodes, and trematodes, were e...

journal_title：Journal of clinical microbiology

authors： Truant AL,Elliott SH,Kelly MT,Smith JH

更新日期：1981-05-01 00:00:00