Cytomegalovirus quantitation by real-time PCR is unaffected by delayed separation of plasma from whole blood.

abstract：:The conventional phage typing scheme proposed by S. Basu and S. Mukerjee (Experientia 24:299-300, 1968) has been used routinely for identification of the strains at the Vibrio Phage Reference Laboratory since 1968. However, because of limitations of this scheme, a new phage typing scheme using five newly isolated phag...

journal_title：Journal of clinical microbiology

authors： Chattopadhyay DJ,Sarkar BL,Ansari MQ,Chakrabarti BK,Roy MK,Ghosh AN,Pal SC

更新日期：1993-06-01 00:00:00

abstract：:Cultures of Sporothrix schenckii were serologically tested by the exoantigen immunodiffusion technique of Kaufman and Standard (L. Kaufman and P.G. Standard, J. Clin. Microbiol. 8:42-45, 1978). This rapid and sensitive technique permitted the identification of 10 isolates of S. schenckii within 3 days. The production ...

journal_title：Journal of clinical microbiology

authors： Polonelli L,Morace G

更新日期：1982-03-01 00:00:00

abstract：:The usefulness of Candida ID 2 (CAID2) reformulated medium (bioMérieux, France) has been compared with that of the former Candida ID (CAID; bioMérieux), Albicans ID 2 (ALB2; bioMérieux), and CHROMagar Candida (CAC; Chromagar, France) chromogenic media for the isolation and presumptive identification of clinically rele...

journal_title：Journal of clinical microbiology

authors： Eraso E,Moragues MD,Villar-Vidal M,Sahand IH,González-Gómez N,Pontón J,Quindós G

更新日期：2006-09-01 00:00:00

abstract：:Nosocomial vancomycin-resistant Enterococcus (VRE) infections have been described in only small numbers of pediatric patients. In none of these studies were multivariate analyses performed to assess which factors were independent risk factors in these patients. In the present cohort study of patients admitted to our h...

journal_title：Journal of clinical microbiology

authors： Singh-Naz N,Sleemi A,Pikis A,Patel KM,Campos JM

更新日期：1999-02-01 00:00:00

abstract：:Lipooligosaccharide configurations were predicted in nontypeable Haemophilus influenzae isolates based on the presence of seven oligosaccharide extension-initiating genes (or alleles). Predicted configurations with 2 to 3 oligosaccharide extensions were more prevalent among middle ear than throat strains. In addition,...

journal_title：Journal of clinical microbiology

authors： McCrea KW,Xie J,Daniel D,Ulrich-Lewis JT,Zhang L

更新日期：2014-07-01 00:00:00

abstract：:A total of 151 Escherichia coli strains resistant to cefotaxime and ceftazidime were isolated during a prospective surveillance study. These strains were characterized by clinical, microbiological, and molecular analyses and were distributed into four clusters of 103, 11, 6, and 5 isolates, along with 25 unrelated str...

journal_title：Journal of clinical microbiology

authors： Oteo J,Navarro C,Cercenado E,Delgado-Iribarren A,Wilhelmi I,Orden B,García C,Miguelañez S,Pérez-Vázquez M,García-Cobos S,Aracil B,Bautista V,Campos J

更新日期：2006-07-01 00:00:00

abstract：:In total, 521 nonrespiratory specimens (91 urine, 30 gastric aspirate, 245 tissue, 113 pleural fluid, 19 cerebrospinal fluid [CSF], and 23 stool specimens) submitted to the German National Reference Laboratory for Mycobacteria (NRL) from May 2009 to August 2010 were comparatively investigated with the new molecular-ba...

journal_title：Journal of clinical microbiology

authors： Hillemann D,Rüsch-Gerdes S,Boehme C,Richter E

更新日期：2011-04-01 00:00:00

abstract：:Immune pressure on lentiviruses exerted by cytotoxic T lymphocytes (CTL) selects for virus CTL epitope mutations. Currently employed methods for monitoring emerging CTL epitope mutations rely on the labor-intensive and time-consuming techniques of virus population or clonal sequencing. Here we describe the development...

journal_title：Journal of clinical microbiology

authors： Peyerl FW,Barouch DH,Bazick HS,Manuel E,Letvin NL

更新日期：2005-09-01 00:00:00

abstract：:The Binax NOW assay (Binax, Inc., Portland, Maine) and the BD Directigen EZ assay (Becton Dickinson and Company, Sparks, Md.), two new rapid immunoassays for detection of respiratory syncytial virus (RSV), as well as the BD Directigen RSV assay (DRSV) (Becton Dickinson and Company) and direct immunofluorescence staini...

journal_title：Journal of clinical microbiology

authors： Ohm-Smith MJ,Nassos PS,Haller BL

更新日期：2004-07-01 00:00:00

abstract：:In a prospective study of 16 patients with Legionnaires disease confirmed by cultural isolation of Legionella pneumophila from the respiratory tract, 38% (6 of 16) had positive blood cultures. Daily subcultures were made onto buffered charcoal-yeast extract plates from 6B aerobic and 7C anaerobic BACTEC blood culture ...

journal_title：Journal of clinical microbiology

authors： Rihs JD,Yu VL,Zuravleff JJ,Goetz A,Muder RR

更新日期：1985-09-01 00:00:00

abstract：:A microagglutination test for Francisella tularensis, with 0.025-ml amounts of diluted sera and 0.025-ml amounts of safranin-O-stained antigen in U-bottom microtitration plates, was compared with a tube agglutination test by using 137 sera. There was 86.3% agreement (+/- 1 dilution variation) between the microagglutin...

journal_title：Journal of clinical microbiology

authors： Brown SL,McKinney FT,Klein GC,Jones WL

更新日期：1980-02-01 00:00:00

abstract：:Campylobacter species are the leading agents of bacterial gastroenteritis in developed countries. In this study 320 specimens of feces from patients with symptoms of acute gastroenteritis were cultured for Campylobacter species by direct plating on modified charcoal cefoperazone deoxycholate agar and by enrichment in ...

journal_title：Journal of clinical microbiology

authors： Maher M,Finnegan C,Collins E,Ward B,Carroll C,Cormican M

更新日期：2003-07-01 00:00:00

abstract：:In summary, it is clear that in vitro susceptibility testing can predict outcome in selected clinical situations. The clearest data are from the fluconazole-treated AIDS patients with oropharyngeal candidiasis. In this setting, the homogeneity of the underlying immune defect, combined with the ease of identification a...

journal_title：Journal of clinical microbiology

authors： Ghannoum MA,Rex JH,Galgiani JN

更新日期：1996-03-01 00:00:00

abstract：:Multidrug-resistant tuberculous meningitis is fatal without rapid diagnosis and use of second-line therapy. It is more common in human immunodeficiency virus (HIV)-positive patients. Beijing genotype strains of Mycobacterium tuberculosis are associated with drug resistance, particularly multidrug resistance, and their...

journal_title：Journal of clinical microbiology

authors： Caws M,Thwaites G,Stepniewska K,Nguyen TN,Nguyen TH,Nguyen TP,Mai NT,Phan MD,Tran HL,Tran TH,van Soolingen D,Kremer K,Nguyen VV,Nguyen TC,Farrar J

更新日期：2006-11-01 00:00:00

abstract：:Current nucleic acid amplification methods to detect Mycobacterium tuberculosis are complex, labor-intensive, and technically challenging. We developed and performed the first analysis of the Cepheid Gene Xpert System's MTB/RIF assay, an integrated hands-free sputum-processing and real-time PCR system with rapid on-de...

journal_title：Journal of clinical microbiology

authors： Helb D,Jones M,Story E,Boehme C,Wallace E,Ho K,Kop J,Owens MR,Rodgers R,Banada P,Safi H,Blakemore R,Lan NT,Jones-López EC,Levi M,Burday M,Ayakaka I,Mugerwa RD,McMillan B,Winn-Deen E,Christel L,Dailey P,Perkins

更新日期：2010-01-01 00:00:00

abstract：:The incidence of antimicrobial-resistant Neisseria gonorrhoeae continues to rise in Canada; however, antimicrobial resistance data are lacking for approximately 70% of gonorrhea infections that are diagnosed directly from clinical specimens by nucleic acid amplification tests (NAATs). We developed a molecular assay fo...

journal_title：Journal of clinical microbiology

authors： Peterson SW,Martin I,Demczuk W,Bharat A,Hoang L,Wylie J,Allen V,Lefebvre B,Tyrrell G,Horsman G,Haldane D,Garceau R,Wong T,Mulvey MR

更新日期：2015-07-01 00:00:00

abstract：:A procedure was developed for characterization of Chlamydia trachomatis strains by using restriction endonuclease analysis of amplified genes of the major outer membrane protein (MOMP). Reference strains of the 15 serovars (A through K and L1 through L3) and clinical isolates were tested. The nucleotide sequences of t...

journal_title：Journal of clinical microbiology

authors： Rodriguez P,Vekris A,de Barbeyrac B,Dutilh B,Bonnet J,Bebear C

更新日期：1991-06-01 00:00:00

abstract：:The name "Campylobacter hyointestinalis" sp. nov. is proposed for a Campylobacter species that was isolated from the intestines of pigs with proliferative enteritis. "C. hyointestinalis" is also found in the feces of cattle and has been isolated from the intestine of a hamster. "C. hyointestinalis" is distinguished fr...

journal_title：Journal of clinical microbiology

authors： Gebhart CJ,Edmonds P,Ward GE,Kurtz HJ,Brenner DJ

更新日期：1985-05-01 00:00:00

abstract：:Vaginal discharge specimens from 105 women were examined by wet mount, culture, and a new direct immunofluorescence assay to detect Trichomonas vaginalis. The organism was detected by culture in 31 patients, by direct immunofluorescence in 26 patients, and by wet mount in 21 patients. ...

journal_title：Journal of clinical microbiology

authors： Smith RF

更新日期：1986-12-01 00:00:00

abstract：:We investigated the presence of Chlamydia pneumoniae in 81 normal and pathological specimens obtained from postmortem brain tissues of patients with multiple sclerosis and with other neurological or nonneurological diseases. The assays used included PCR amplification of all DNA samples in the initial study. Culture an...

journal_title：Journal of clinical microbiology

authors： Hammerschlag MR,Ke Z,Lu F,Roblin P,Boman J,Kalman B

更新日期：2000-11-01 00:00:00

abstract：:Brucella melitensis is the cause of brucellosis in sheep and goats, which often results in abortion. Few cases of B. melitensis infection in goats have occurred in the United States over the last 25 years. However, vigilance must be maintained, as it is for the bovine milk industry, to ensure that brucellosis is not i...

journal_title：Journal of clinical microbiology

authors： Funk ND,Tabatabai LB,Elzer PH,Hagius SD,Martin BM,Hoffman LJ

更新日期：2005-02-01 00:00:00

abstract：:We surveyed 75 clinical laboratories to determine if National Committee for Clinical Laboratory Standards (NCCLS) were being used for the susceptibility testing of Haemophilus influenzae. Of the 66 laboratories that performed susceptibility testing, all claimed to follow current NCCLS guidelines. However, upon further...

journal_title：Journal of clinical microbiology

authors： Scriver SR,Hoban DJ,McGeer A,Moore TC,Walmsley SL,Low DE

更新日期：1994-08-01 00:00:00

abstract：:In order to investigate the seroprevalence of human herpesvirus 8 (HHV-8) infection in central and southern Italy, sera from human immunodeficiency virus (HIV)-seronegative subjects, with and without Kaposi's sarcoma (KS), were analyzed by immunofluorescence assay, using BC-3, a cell line latently infected with HHV-8....

journal_title：Journal of clinical microbiology

authors： Cattani P,Capuano M,Cerimele F,La Parola IL,Santangelo R,Masini C,Cerimele D,Fadda G

更新日期：1999-04-01 00:00:00

abstract：:Trichosporon species are relevant etiologic agents of hospital-acquired infections. High mortality rates are attributed to Trichosporon deep-seated infections in immunocompromised individuals, making fast and accurate species identification relevant for hastening the discovery of best-targeted therapy. Recently, Trich...

journal_title：Journal of clinical microbiology

authors： de Almeida JN Jr,Favero Gimenes VM,Francisco EC,Machado Siqueira LP,Gonçalves de Almeida RK,Guitard J,Hennequin C,Colombo AL,Benard G,Rossi F

更新日期：2017-08-01 00:00:00

abstract：:Adhesin-encoding operons (pap, sfa/foc, and afa) have been shown to be prevalent in Escherichia coli strains associated with urinary tract infections. A quick and sensitive assay to identify these operons was developed by using the polymerase chain reaction (PCR). Three pairs of 25-mer primers were defined from the se...

journal_title：Journal of clinical microbiology

authors： Le Bouguenec C,Archambaud M,Labigne A

更新日期：1992-05-01 00:00:00

abstract：:A total of 215 typical strains of Candida albicans were studied for their ability to assimilate 11 carbon compounds. All isolates assimilated lactic acid, ribitol, succinic acid, methyl alpha-D-glucopyranoside. None of the isolates assimilated cellobiose and salicin; 1.9% of the isolates assimilated L-arabinose. Citri...

journal_title：Journal of clinical microbiology

authors： Syverson RE

更新日期：1981-01-01 00:00:00

abstract：:In this study, disk diffusion testing with ceftizoxime and cefuroxime was evaluated for use in predicting the susceptibility of Streptococcus pneumoniae to ceftriaxone and cefotaxime. Of the 194 isolates included in this study, 138 were susceptible, 34 were intermediate, and 22 were resistant to cefotaxime by MIC test...

journal_title：Journal of clinical microbiology

authors： Williams-Bouyer N,Hernandez A,Reisner BS

更新日期：1999-11-01 00:00:00

abstract：:Sixty-six Corynebacterium diphtheriae strains (62 of the gravis biotype and 4 of the mitis biotype) isolated during the Georgian diphtheria epidemic of 1993 to 1998 and 13 non-Georgian C. diphtheriae strains (10 Russian and 3 reference isolates) were characterized by (i) biotyping, (ii) toxigenicity testing with the E...

journal_title：Journal of clinical microbiology

authors： Sulakvelidze A,Kekelidze M,Gomelauri T,Deng Y,Khetsuriani N,Kobaidze K,De Zoysa A,Efstratiou A,Morris JG Jr,Imnadze P

更新日期：1999-10-01 00:00:00

abstract：:We determined the types of cholera toxin (CT) produced by a collection of 185 Vibrio cholerae O1 strains isolated in Bangladesh over the past 45 years. All of the El Tor strains of V. cholerae O1 isolated since 2001 produced CT of the classical biotype, while those isolated before 2001 produced CT of the El Tor biotyp...

journal_title：Journal of clinical microbiology

authors： Nair GB,Qadri F,Holmgren J,Svennerholm AM,Safa A,Bhuiyan NA,Ahmad QS,Faruque SM,Faruque AS,Takeda Y,Sack DA

更新日期：2006-11-01 00:00:00

abstract：:Three forms of DNA-based typing procedures for Aspergillus fumigatus isolates have been developed over the last five years. The procedures are random amplification of polymorphic DNA (RAPD), restriction fragment length polymorphism (RFLP) detection, and Southern hybridizations with various repetitive sequence-based pr...

journal_title：Journal of clinical microbiology

authors： Anderson MJ,Gull K,Denning DW

更新日期：1996-01-01 00:00:00