Human herpesvirus 8 seroprevalence and evaluation of nonsexual transmission routes by detection of DNA in clinical specimens from human immunodeficiency virus-seronegative patients from central and southern Italy, with and without Kaposi's sarcoma.

abstract：:Monitoring antiretroviral therapy requires that human immunodeficiency virus type 1 (HIV-1) viremia assays are applicable to all distinct variants. This study evaluates the performance of three commercial viral load assays-Versant HIV-1 RNA bDNA v3.0, Cobas AmpliPrep/Cobas TaqMan HIV-1, and NucliSens HIV-1 EasyQ v1.2-...

journal_title：Journal of clinical microbiology

authors： Holguín A,López M,Molinero M,Soriano V

更新日期：2008-09-01 00:00:00

abstract：:We report in this work a highly sensitive and nonradioactive PCR method for the detection of the four species of parasite causing human malaria. Plasmodium-specific primers corresponding to the small-subunit rRNA genes of the malaria parasite were used, and a 291-bp fragment was amplified. Our results showed a high sp...

journal_title：Journal of clinical microbiology

authors： Ciceron L,Jaureguiberry G,Gay F,Danis M

更新日期：1999-01-01 00:00:00

abstract：:Rapid detection of mycobacterial disease is essential. Using multiple specimen types and concentrations of mycobacteria, we compared two commercial auramine O stains. The more rapid stain permitted consistent acid-fast bacillus quantitation and exhibited less debris staining, and the staining procedure required less t...

journal_title：Journal of clinical microbiology

authors： Hendry C,Dionne K,Hedgepeth A,Carroll K,Parrish N

更新日期：2009-04-01 00:00:00

abstract：:The etiologic role of oral treponemes in human periodontitis is still under debate. Although seen by dark-field microscopy in large numbers, their possible role is still unclear since they comprise some 60 different phylotypes, most of which are still uncultured. To determine their status as mere commensals or opportu...

journal_title：Journal of clinical microbiology

authors： Moter A,Riep B,Haban V,Heuner K,Siebert G,Berning M,Wyss C,Ehmke B,Flemmig TF,Göbel UB

更新日期：2006-09-01 00:00:00

abstract：:A newly developed enzyme immunoassay (EIA) was used to detect the presence of pneumococcal teichoic and lipoteichoic acids in cerebrospinal fluid (CSF) from patients with Streptococcus pneumoniae meningitis who were being treated with antibiotics. All initial CSF samples, which on culture grew S. pneumoniae, were posi...

journal_title：Journal of clinical microbiology

authors： Stuertz K,Merx I,Eiffert H,Schmutzhard E,Mäder M,Nau R

更新日期：1998-08-01 00:00:00

abstract：:Knowledge of polyomavirus BK (BKV) genomic diversity has greatly expanded. The implications of BKV DNA sequence variation for the performance of molecular diagnostic assays is not well studied. We analyzed 184 publically available VP-1 sequences encompassing the BKV genomic region targeted by an in-house quantitative ...

journal_title：Journal of clinical microbiology

authors： Randhawa P,Kant J,Shapiro R,Tan H,Basu A,Luo C

更新日期：2011-12-01 00:00:00

abstract：:Genetic relationships within ureaplasma serovars were investigated by pulsed-field gel electrophoresis (PFGE). One hundred thirteen Ureaplasma parvum isolates and 78 Ureaplasma urealyticum isolates were different from their ATCC serovar type strains and different within the same serovars. The organisms were geographic...

journal_title：Journal of clinical microbiology

authors： Xiao L,Crabb DM,Moser SA,Duffy LB,Glass JI,Paralanov V,Waites KB

更新日期：2011-09-01 00:00:00

abstract：:We used simulated blood cultures inoculated with clinical isolates of Mycoplasma hominis to determine whether liquid media of the BacT/ALERT (Organon Teknika, Durham, N.C.) will support growth of this fastidious organism and whether its presence can generate a positive signal with the instrument. Viability of clinical...

journal_title：Journal of clinical microbiology

authors： Waites KB,Canupp KC

更新日期：2001-12-01 00:00:00

abstract：:Antiserum to a purified type R lipopolysaccharide antigen isolated from Neisseria gonorrhoeae was used in a slide agglutination test and compared with conventional carbohydrate utilization and fluorescent antibody tests to confirm the identity of laboratory cultures classified as typical or "atypical" N. gonorrhoeae. ...

journal_title：Journal of clinical microbiology

authors： Arko RJ,Wong KH,Peacock WL

更新日期：1979-04-01 00:00:00

abstract：:Imferon, an iron-dextran complex, enhances the growth of Neisseria gonorrhoeae and N. meningitidis. The use of Imferon as a replacement for ferric nitrate, in a defined supplement for GC agar significantly increased the average colony sizes of both gonococci and meningococci. In comparison with Thayer-Martin medium, I...

journal_title：Journal of clinical microbiology

authors： Payne SM,Finkelstein RA

更新日期：1977-09-01 00:00:00

abstract：:Sequence analysis of 58 multidrug-resistant Mycobacterium tuberculosis complex strains from Germany and 55 susceptible strains from a reference collection comprising major phylogenetic lineages confirmed that variations in Rv2629, 191A/C and 965C/T, are specific for genotypes Beijing and Ghana, respectively, but not i...

journal_title：Journal of clinical microbiology

authors： Homolka S,Köser C,Archer J,Rüsch-Gerdes S,Niemann S

更新日期：2009-01-01 00:00:00

abstract：:During a previous survey, five extended-spectrum beta-lactamase (ESBL)-producing enterobacteria (ESBLE) (two Enterobacter aerogenes isolates expressing TEM-24 b, two Escherichia coli isolates expressing TEM-21 or TEM-24 b, and one Klebsiella pneumoniae isolate expressing SHV-4/TEM-15) responsible for urinary tract inf...

journal_title：Journal of clinical microbiology

authors： Arpin C,Dubois V,Maugein J,Jullin J,Dutilh B,Brochet JP,Larribet G,Fischer I,Quentin C

更新日期：2005-10-01 00:00:00

abstract：:The nucleotide sequences of the partial rpoB gene were determined from 38 Legionella species, including 15 serogroups of Legionella pneumophila. These sequences were then used to infer the phylogenetic relationships among the Legionella species in order to establish a molecular differentiation method appropriate for t...

journal_title：Journal of clinical microbiology

authors： Ko KS,Lee HK,Park MY,Lee KH,Yun YJ,Woo SY,Miyamoto H,Kook YH

更新日期：2002-07-01 00:00:00

abstract：:A total of 101 isolates of penicillinase-producing and non-penicillinase-producing Neisseria gonorrhoeae with known nutritional requirements, plasmid content, and serovars, were examined for lectin agglutination patterns. These isolates were from outbreaks in Georgia, California, Hawaii, and Pennsylvania. Cell suspens...

journal_title：Journal of clinical microbiology

authors： Schalla WO,Whittington WL,Rice RJ,Larsen SA

更新日期：1985-09-01 00:00:00

abstract：:Standard broth microdilution (with and without bovine serum albumin [BSA] supplementation), tube dilution, and agar dilution susceptibility tests were compared for determining ramoplanin MICs. With a data base of 246 clinical isolates of gram-positive bacteria from 33 U.S. sites, it was shown that (i) agar and tube di...

journal_title：Journal of clinical microbiology

authors： Kenny MT,Brackman MA

更新日期：1994-05-01 00:00:00

abstract：:Reference isolates of Mycobacterium neoaurum, Mycobacterium aurum, and the nonvalidated species "Mycobacterium lacticola" were the focus of two recent molecular taxonomic studies. On the basis of this grouping, we identified 46 clinical pigmented, rapidly growing mycobacterial isolates. By 16S rRNA gene sequencing, on...

journal_title：Journal of clinical microbiology

authors： Brown-Elliott BA,Wallace RJ Jr,Petti CA,Mann LB,McGlasson M,Chihara S,Smith GL,Painter P,Hail D,Wilson R,Simmon KE

更新日期：2010-12-01 00:00:00

abstract：:The use of IS6110 as a marker for molecular epidemiological studies is limited when a Mycobacterium tuberculosis isolate has five or fewer copies of IS6110. Restriction fragment length polymorphism analysis with a highly polymorphic GC-rich repetitive sequence located in the plasmid pTBN12 (PGRS RFLP) and spoligotypin...

journal_title：Journal of clinical microbiology

authors： Flores L,Jarlsberg LG,Kim EY,Osmond D,Grinsdale J,Kawamura M,Desmond E,Hopewell PC,Kato-Maeda M

更新日期：2010-02-01 00:00:00

abstract：:A protocol was optimized for the isolation of Mycobacterium avium subsp. paratuberculosis (MAP) from milk and colostrum, with parameters including chemical decontamination, antibiotics, and different culture media. This study demonstrates that the efficiency of MAP recovery from milk is highly dependent upon the cultu...

journal_title：Journal of clinical microbiology

authors： Bradner L,Robbe-Austerman S,Beitz DC,Stabel JR

更新日期：2013-05-01 00:00:00

abstract：:Seventeen Minnesota and Wisconsin dogs with granulocytic ehrlichosis were studied. The diagnoses were made by finding ehrlichia morulae in peripheral blood neutrophils. Eight dogs were studied retrospectively, and nine dogs were studied prospectively. The medical records of all dogs were reviewed. Eighty-eight percent...

journal_title：Journal of clinical microbiology

authors： Greig B,Asanovich KM,Armstrong PJ,Dumler JS

更新日期：1996-01-01 00:00:00

abstract：:During a 15-month period, two methods for detection of herpes simplex virus (HSV) in 699 clinical specimens were compared: (i) 24-well-plate centrifugation (24WPC) with MRC-5 cells and staining with type-specific monoclonal antibodies (Syva Co., Palo Alto, Calif.) after incubation for 16 to 18 h and (ii) conventional ...

journal_title：Journal of clinical microbiology

authors： Woods GL,Mills RD

更新日期：1988-03-01 00:00:00

abstract：:A bacitracin (0.04 units, BBL)-SXT (trimethoprim, 1.25 mg, plus sulfamethoxazole, 23.75 mg, BBL) susceptibility test was 94% accurate in presumptively identifying streptococci as either group A, B or not group A and B. ...

journal_title：Journal of clinical microbiology

authors： Gunn BA

更新日期：1976-08-01 00:00:00

abstract：:Of 100 strains of enterococci, 83% reduced litmus milk within 4 h. None of the 100 non-enterococcal streptococci tested were positive. ...

journal_title：Journal of clinical microbiology

authors： Schierl EA,Blazevic DJ

更新日期：1981-08-01 00:00:00

abstract：:We examined several factors for their effects on the serological reactivity of Legionella antigens used for direct or indirect fluorescent-antibody tests. These factors included media, methods of killing, strain differences, and the nature of the reactivity with diverse human sera. The maximum serological reactivities...

journal_title：Journal of clinical microbiology

authors： Benson RF,Malcolm GB,Pine L,Harrell WK

更新日期：1983-05-01 00:00:00

abstract：:Rapid tests for glucose, maltose, lactose, sucrose, and starch fermentation were performed on 112 strains of anaerobic bacteria. The tests were incubated under aerobic conditions, and results were read within 4 h. An overall correlation of 89% was achieved between the rapid tests and the Virginia Polytechnic Institut...

journal_title：Journal of clinical microbiology

authors： Schreckenberger PC,Blazevic DJ

更新日期：1976-03-01 00:00:00

abstract：:For recovery of brucellae from clinical specimens, a lysis-concentration procedure was superior to the procedure of Castañeda (M. R. Castañeda, Proc. Soc. Exp. Biol. Med. 64:114-115, 1947). ...

journal_title：Journal of clinical microbiology

authors： Etemadi H,Raissadat A,Pickett MJ,Zafari Y,Vahedifar P

更新日期：1984-09-01 00:00:00

abstract：:To facilitate the identification and serotyping of Salmonella species, we established a wide variety of murine monoclonal antibodies (MAbs) that were reactive with the lipopolysaccharides (LPSs) of Salmonella serogroups B to E. An effective approach for generating LPS-reactive hybridomas was used; this required immuni...

journal_title：Journal of clinical microbiology

authors： Luk JM,Lindberg AA

更新日期：1991-11-01 00:00:00

abstract：:Specimens from skin lesions were examined simultaneously for herpes simplex virus (HSV) and varicella-zoster virus (VZV) by direct specimen testing and shell vial culture in single-test systems. For direct testing, cells in a single specimen well were stained with a combination direct-indirect immunofluorescence stain...

journal_title：Journal of clinical microbiology

authors： Brumback BG,Farthing PG,Castellino SN

更新日期：1993-12-01 00:00:00

abstract：:The source of human immunodeficiency virus type 1 (HIV-1) RNA in cerebrospinal fluid (CSF) during HIV-1 infection is uncertain. The sequence heterogeneity of HIV-1 RNA in simultaneous CSF and plasma samples was characterized for five patients at the baseline and during the first week of antiretroviral therapy by two c...

journal_title：Journal of clinical microbiology

authors： Tang YW,Huong JT,Lloyd RM Jr,Spearman P,Haas DW

更新日期：2000-12-01 00:00:00

abstract：:Enteroviruses have been reported in encephalitis cases. However, clinical and epidemiological characteristics of enteroviruses in encephalitis are not fully established. We prospectively investigated 204 children with encephalitis over a period of 2 years (2009 to 2010) for enterovirus. Enterovirus was detected in 45 ...

journal_title：Journal of clinical microbiology

authors： Kumar A,Shukla D,Kumar R,Idris MZ,Misra UK,Dhole TN

更新日期：2012-11-01 00:00:00

abstract：:The extent of genetic and antigenic variation found in a population of human parainfluenza virus type 1 (HPIV-1) during a single local epidemic was investigated. Fifteen HPIV-1 strains isolated from children in 1991 were analyzed. Nucleotide sequence variation in the hemagglutinin-neuraminidase protein (HN) gene demon...

journal_title：Journal of clinical microbiology

authors： Henrickson KJ,Savatski LL

更新日期：1996-03-01 00:00:00