Geographic, clinical, serologic, and molecular evidence of granulocytic ehrlichiosis, a likely zoonotic disease, in Minnesota and Wisconsin dogs.

abstract：:The frequency with which clue cells could be detected in Gram-stained vaginal smears and/or cervical Papanicolaou (Pap) smears was compared with the frequency of Corynebacterium vaginale (Haemophilus vaginalis) isolation in a group of 236 female patients, of whom 221 had vaginitis. Vaginal clue cells were found most o...

journal_title：Journal of clinical microbiology

authors： Smith RF,Rodgers HA,Hines PA,Ray RM

更新日期：1977-03-01 00:00:00

abstract：:Mupirocin is a topical antimicrobial used to eradicate methicillin-resistant Staphylococcus aureus (MRSA) colonization, usually in the absence of susceptibility testing. We hypothesized that high-level (HL) mupirocin resistance was associated with multidrug resistance (MDR). To this end, unique patient isolates identi...

journal_title：Journal of clinical microbiology

authors： Cadilla A,David MZ,Daum RS,Boyle-Vavra S

更新日期：2011-01-01 00:00:00

abstract：:We prospectively evaluated a new PCR-enzyme-linked immunosorbent assay kit (Onychodiag; BioAdvance, France) for the diagnosis of dermatophytic onychomycosis by testing nail samples from 438 patients with suspected onychomycosis and from 108 healthy controls in three independent laboratories. In two laboratories, sampl...

journal_title：Journal of clinical microbiology

authors： Savin C,Huck S,Rolland C,Benderdouche M,Faure O,Noacco G,Menotti J,Candolfi E,Pelloux H,Grillot R,Coupe S,Derouin F

更新日期：2007-04-01 00:00:00

abstract：:Genetic polymorphisms in the malaria parasite Plasmodium falciparum mediate alterations in sensitivity to important antimalarial drugs. Surveillance for these polymorphisms is helpful in assessing the prevalence of drug resistance and designing strategies for malaria control. Multiple methods are available for the ass...

journal_title：Journal of clinical microbiology

authors： LeClair NP,Conrad MD,Baliraine FN,Nsanzabana C,Nsobya SL,Rosenthal PJ

更新日期：2013-08-01 00:00:00

abstract：:Although TT virus (TTV) was isolated from a cryptogenic posttransfusion hepatitis patient, its pathogenic role remains unclear. It has been reported that the majority of the healthy population is infected with TTV. To elucidate the differences between TTV infection in patients with liver diseases and TTV infection in ...

journal_title：Journal of clinical microbiology

authors： Kato T,Mizokami M,Mukaide M,Orito E,Ohno T,Nakano T,Tanaka Y,Kato H,Sugauchi F,Ueda R,Hirashima N,Shimamatsu K,Kage M,Kojiro M

更新日期：2000-01-01 00:00:00

abstract：:Hafnia alvei strains which possess the attachment-effacement gene (eaeA) may have clinical importance as new diarrhea-causing pathogens and should therefore be differentiated from other H. alvei strains. We characterized diarrheal H. alvei strains, which were positive in the PCR test for the eaeA gene, using biochemic...

journal_title：Journal of clinical microbiology

authors： Ridell J,Siitonen A,Paulin L,Lindroos O,Korkeala H,Albert MJ

更新日期：1995-09-01 00:00:00

abstract：:An inexpensive, rapid, and simple colorimetric test for detection of bacteriuria is described. This test does not require bacterial growth and has the marked advantage of being able to quantify bacteria, even when the organisms are present in the urine of bacteriuric patients who are being treated with antibiotics. Th...

journal_title：Journal of clinical microbiology

authors： Wallis C,Melnick JL,Longoria CJ

更新日期：1981-09-01 00:00:00

abstract：:A new, dried antigen-coated latex preparation for the demonstration of infectious mononucleosis (IM) heterophile antibody (Dryspot IM kit; Oxoid, Ltd., Basingstoke, Hampshire, United Kingdom) was compared with the IM kit (a liquid latex reagent from the same source), an immunoassay (ImmunoCard Mono; Meridian Diagnosti...

journal_title：Journal of clinical microbiology

authors： Rogers R,Windust A,Gregory J

更新日期：1999-01-01 00:00:00

abstract：:Although it has been recommended that photochromogenicity of mycobacteria should be tested after incubation at 25 to 30 degrees C for 2 weeks (Wayne et al., Int. J. Syst. Bacteriol. 24:412-419, 1974), our results indicate that photochromogenicity should be determined at 37 degrees C in the dark and under continuous ex...

journal_title：Journal of clinical microbiology

authors： Tsukamura M

更新日期：1981-08-01 00:00:00

abstract：:Rickettsial proteins rOmp A and rOmp B exist in both Rickettsia australis and Rickettsia honei but differ in molecular weight and antigenicity; in addition, they produce distinct immunogenic responses and appear to be to conformationally dependent antigens. Species-specific monoclonal antibodies for other spotted feve...

journal_title：Journal of clinical microbiology

authors： Stenos J,Ross B,Feng HM,Crocquet-Valdes P,Walker D

更新日期：1997-01-01 00:00:00

abstract：:Fimbriae of Porphyromonas gingivalis are filamentous appendages on the cell surface and are thought to be one of the virulence factors. The fimA gene encoding the subunit protein of fimbriae, fimbrillin (FimA), was classified into four typeable variants (types I to IV). We previously examined the distribution of P. gi...

journal_title：Journal of clinical microbiology

authors： Nakagawa I,Amano A,Kimura RK,Nakamura T,Kawabata S,Hamada S

更新日期：2000-05-01 00:00:00

abstract：:We report a nonfatal case of streptococcal toxic shock syndrome (STSS) caused by a Streptococcus pyogenes emm118 strain encoding a novel variant of streptococcal mitogenic exotoxin Z (SMEZ-34). This variant was responsible for the major mitogenic activity in the cell culture supernatant. Patient sera showed seroconver...

journal_title：Journal of clinical microbiology

authors： Yang L,Thomas M,Woodhouse A,Martin D,Fraser JD,Proft T

更新日期：2005-07-01 00:00:00

abstract：:Cryptosporidium parvum and Cryptosporidium hominis isolates from human immunodeficiency virus-infected patients, cattle, and wild ruminants were characterized by PCR and DNA sequencing analysis of the 60-kDa glycoprotein gene. Seven alleles were identified, three corresponding to C. hominis and four corresponding to C...

journal_title：Journal of clinical microbiology

authors： Alves M,Xiao L,Sulaiman I,Lal AA,Matos O,Antunes F

更新日期：2003-06-01 00:00:00

abstract：:Rapid pathogen identification can alter antibiotic prescribing practices if interpreted correctly. Microbiology reporting can be difficult to understand, and new technology has made it more challenging. Nebraska Medicine recently implemented the BioFire FilmArray blood culture identification panel (BCID) coupled with ...

journal_title：Journal of clinical microbiology

authors： Donner LM,Campbell WS,Lyden E,Van Schooneveld TC

更新日期：2017-05-01 00:00:00

abstract：:A simplified procedure for serotyping Campylobacter jejuni and Campylobacter coli on the basis of thermostable antigens was developed and tested for its applicability as a routine typing method. The assay involves the sensitization of erythrocytes with an antigenic extract and performance of a slide agglutination assa...

journal_title：Journal of clinical microbiology

authors： Mills SD,Congi RV,Hennessy JN,Penner JL

更新日期：1991-10-01 00:00:00

abstract：:Herpes infections are among the most common sexually transmitted diseases and are the most common cause of genital ulcer disease in the United States. This study addresses the changing distribution of herpes simplex virus type 1 (HSV-1) and HSV-2 in patients presenting for evaluation of herpetic infections. Viral cult...

journal_title：Journal of clinical microbiology

authors： Ribes JA,Steele AD,Seabolt JP,Baker DJ

更新日期：2001-09-01 00:00:00

abstract：:Of 382 strains tested, 261 strains of the species Bacteroides fragilis, B. thetaiotaomicron, B. ovatus, and B. distasonis produced phenylacetic acid; the remaining strains, belonging exclusively to the species B. vulgatus, failed to do so. This differentiation characteristic may be useful in routine clinical bacteriol...

journal_title：Journal of clinical microbiology

authors： van Assche PF

更新日期：1978-11-01 00:00:00

abstract：:Fluorophore-assisted carbohydrate electrophoresis (FACE) is a straightforward, sensitive method for determining the presence and relative abundance of individual oligomannosyl residues in Candida mannoprotein, the major antigenic determinant located on the outer surface of the yeast cell wall. The single terminal alde...

journal_title：Journal of clinical microbiology

authors： Goins TL,Cutler JE

更新日期：2000-08-01 00:00:00

abstract：:The duration of mumps virus RNA detection was studied during a mumps outbreak in a highly vaccinated university population. Seven of the eight reverse transcription-PCR-positive specimens were collected during the first 3 days of parotitis, suggesting that viral shedding is minimal after the first 3 days of symptoms. ...

journal_title：Journal of clinical microbiology

authors： Bitsko RH,Cortese MM,Dayan GH,Rota PA,Lowe L,Iversen SC,Bellini WJ

更新日期：2008-03-01 00:00:00

abstract：:A procedure was developed for characterization of Chlamydia trachomatis strains by using restriction endonuclease analysis of amplified genes of the major outer membrane protein (MOMP). Reference strains of the 15 serovars (A through K and L1 through L3) and clinical isolates were tested. The nucleotide sequences of t...

journal_title：Journal of clinical microbiology

authors： Rodriguez P,Vekris A,de Barbeyrac B,Dutilh B,Bonnet J,Bebear C

更新日期：1991-06-01 00:00:00

abstract：:A novel PCR primer pair was used to detect the presence of cmeC in 131 Campylobacter jejuni and C. coli strains isolated from various hosts (cattle, turkeys, humans, and pigs). DNA sequence analysis revealed a high degree of genetic variation between the two species, while extremely limited genetic variation among iso...

journal_title：Journal of clinical microbiology

authors： Fakhr MK,Logue CM

更新日期：2007-10-01 00:00:00

abstract：:The OmpK36 porin plays a role in carbapenem resistance and may contribute to bacterial virulence in Klebsiella pneumoniae. This study aimed to investigate the characteristics of different groups of K. pneumoniae separated by ompK36 typing. Among 226 nonduplicate K. pneumoniae bloodstream isolates collected at a Taiwan...

journal_title：Journal of clinical microbiology

authors： Yan JJ,Zheng PX,Wang MC,Tsai SH,Wang LR,Wu JJ

更新日期：2015-10-01 00:00:00

abstract：:Testing for susceptibility to pyrazinamide (PZA) and analysis of the pncA gene sequences of 423 Mycobacterium tuberculosis complex isolates have revealed a unique silent nucleotide substitution that enables the rapid identification of "M. canettii" (proposed name). Moreover, the lack of a defined mutation within the p...

journal_title：Journal of clinical microbiology

authors： Somoskovi A,Dormandy J,Parsons LM,Kaswa M,Goh KS,Rastogi N,Salfinger M

更新日期：2007-02-01 00:00:00

abstract：:We describe a method for the simultaneous identification of up to three Mycoplasma species by the use of contrast-labeled fluorescent antibodies against two species and peroxidase-labeled antibody against a third species. The procedure enabled the rapid identification of colonies of three artificially mixed avian Myco...

journal_title：Journal of clinical microbiology

authors： Bencina D,Bradbury JM

更新日期：1992-02-01 00:00:00

abstract：:We retrospectively compared the utility of a fungal isolation device (Isolator) versus conventional techniques for recovering fungal organisms from blood cultures obtained from neutropenic cancer patients. Positive cultures were deemed true pathogens, possible pathogens, or contaminants according to laboratory and cli...

journal_title：Journal of clinical microbiology

authors： Creger RJ,Weeman KE,Jacobs MR,Morrissey A,Parker P,Fox RM,Lazarus HM

更新日期：1998-01-01 00:00:00

abstract：:The development of a rhinovirus (RV)-RNA-specific reverse transcription (RT)-PCR assay is complicated by the close homology between the RV and enterovirus (EV) genomes in the highly conserved 5'-noncoding region, which is chosen for primer design in most RT-PCR assays. We have developed a sensitive, rapid, and RV-spec...

journal_title：Journal of clinical microbiology

authors： Steininger C,Aberle SW,Popow-Kraupp T

更新日期：2001-01-01 00:00:00

abstract：:The utility of the GeneXpert MTB/RIF (Xpert) assay for detection of Mycobacterium tuberculosis in sputum samples has been extensively studied. However, the performance of the Xpert assay as applied to other readily accessible body fluids such as exhaled breath condensate (EBC), saliva, urine, and blood has not been es...

journal_title：Journal of clinical microbiology

authors： Shenai S,Amisano D,Ronacher K,Kriel M,Banada PP,Song T,Lee M,Joh JS,Winter J,Thayer R,Via LE,Kim S,Barry CE 3rd,Walzl G,Alland D

更新日期：2013-12-01 00:00:00

abstract：:Survival of anaerobic and aerobic bacteria in a commercially available, non-supportive, gassed (oxygen-free) transport container (Anaport) was evaluated quantitatively. Saline-suspended obligate anaerobes survived significantly better in the gassed container in aerobic control tubes (P less than 0.025, t test), and co...

journal_title：Journal of clinical microbiology

authors： Chow AW,Cunningham PJ,Guze LB

更新日期：1976-02-01 00:00:00

abstract：:Pasteurella multocida is a small nonmotile gram-negative coccobacillus that is found in the nasopharynx and gastrointestinal tract of many wild and domesticated animals. In humans it most commonly causes cellulitis and localized superficial skin abscesses following an animal bite or scratch. The respiratory tract is t...

journal_title：Journal of clinical microbiology

authors： Chen HI,Hulten K,Clarridge JE 3rd

更新日期：2002-09-01 00:00:00

abstract：:Multilocus sequence typing was successfully completed on 494 isolates of Streptococcus uberis from clinical mastitis cases in a study of 52 commercial dairy herds over a 12-month period. In total, 195 sequence types (STs) were identified. S. uberis mastitis cases that occurred in different cows within the same herd an...

journal_title：Journal of clinical microbiology

authors： Davies PL,Leigh JA,Bradley AJ,Archer SC,Emes RD,Green MJ

更新日期：2016-01-01 00:00:00