Characterization of Hafnia alvei by biochemical tests, random amplified polymorphic DNA PCR, and partial sequencing of 16S rRNA gene.

abstract：:Rotaviruses contain a double-stranded ribonucleic acid genome consisting of 11 segments. Gel electrophoresis separates genome segments and allows identification of strain differences. This electrophoretic typing technique was applied to rotavirus specimens from 116 children and 72 newborn babies. Between 1973 and 1979...

journal_title：Journal of clinical microbiology

authors： Rodger SM,Bishop RF,Birch C,McLean B,Holmes IH

更新日期：1981-02-01 00:00:00

abstract：:A cytopathic astrovirus was isolated from pigs with acute diarrhea in an established cell line that was derived from porcine embryonic kidneys with the aid of trypsin. The virus showed a distinct cytopathic effect characterized by an enlargement of cells and the appearance of fine granules in the cytoplasm. Porcine as...

journal_title：Journal of clinical microbiology

authors： Shimizu M,Shirai J,Narita M,Yamane T

更新日期：1990-02-01 00:00:00

abstract：:The presence of Helicobacter DNA species has been investigated in the biliary epithelium of patients with biliary diseases. However, conflicting results have been observed that may have been due to the small number of subjects studied, difficulty in obtaining a healthy control group, absence of controlling for confoun...

journal_title：Journal of clinical microbiology

authors： Silva CP,Pereira-Lima JC,Oliveira AG,Guerra JB,Marques DL,Sarmanho L,Cabral MM,Queiroz DM

更新日期：2003-12-01 00:00:00

abstract：:A prospective study of Campylobacter jejuni infection was performed during a 6-month period in a cohort of 198 Chilean infants. Surveillance was based on biweekly home visits by a team of trained nurses. C. jejuni colonization was studied by culturing stool samples from diarrheal episodes and paired asymptomatic contr...

journal_title：Journal of clinical microbiology

authors： Figueroa G,Galeno H,Troncoso M,Toledo S,Soto V

更新日期：1989-05-01 00:00:00

abstract：:The number of reports concerning Escherichia coli clinical isolates that produce glutathione S-transferases responsible for fosfomycin resistance (FR-GSTs) has been increasing. We have developed a disk-based potentiation test in which FR-GST producers expand the growth inhibition zone around a Kirby-Bauer disk contain...

journal_title：Journal of clinical microbiology

authors： Nakamura G,Wachino J,Sato N,Kimura K,Yamada K,Jin W,Shibayama K,Yagi T,Kawamura K,Arakawa Y

更新日期：2014-09-01 00:00:00

abstract：:Streptococcus pneumoniae is both a commensal and a major pathogen that causes invasive disease in people of all ages. The introduction of serotype-specific pneumococcal vaccines has reduced the burden of disease but has also led to replacement with new strains; thus, serotyping remains important for vaccine-related di...

journal_title：Journal of clinical microbiology

authors： Pholwat S,Sakai F,Turner P,Vidal JE,Houpt ER

更新日期：2016-07-01 00:00:00

abstract：:The results of single radial immunodiffusion assays of influenza virus hemagglutinin were found to be greatly altered by small antigenic differences between test and reference strains. When such differences were present, the precise specificity of the antiserum used had a critical effect on the measured hemagglutinin ...

journal_title：Journal of clinical microbiology

authors： Rodda SJ,Gallichio HA,Hampson AW

更新日期：1981-11-01 00:00:00

abstract：:Salmonella enterica subsp. enterica serovar Typhimurium var. Copenhagen isolates from a heifer-raising operation and from 11 dairy herds that had their calves contracted to the heifer-raising operation were examined for their phenotypic and genotypic characteristics. Results of the study showed that the heifer-raising...

journal_title：Journal of clinical microbiology

authors： Hegde NV,Cook ML,Wolfgang DR,Love BC,Maddox CC,Jayarao BM

更新日期：2005-08-01 00:00:00

abstract：:Of 45 influenza virus strains (43 type A and 2 type B) detected in conventional tube cell cultures (average time, 4 days), 25 (56%) were detected by immunofluorescence in the shell vial assay 24 h postinoculation. The specific fluorescence produced should allow this procedure to be readily adapted by laboratories with...

journal_title：Journal of clinical microbiology

authors： Espy MJ,Smith TF,Harmon MW,Kendal AP

更新日期：1986-10-01 00:00:00

abstract：:Whole HIV-1 genome sequences are pivotal for large-scale studies of inter- and intrahost evolution, including the acquisition of drug resistance mutations. The ability to rapidly and cost-effectively generate large numbers of HIV-1 genome sequences from different populations and geographical locations and determine th...

journal_title：Journal of clinical microbiology

authors： Gall A,Ferns B,Morris C,Watson S,Cotten M,Robinson M,Berry N,Pillay D,Kellam P

更新日期：2012-12-01 00:00:00

abstract：:The sequencing of the VP1 hypervariable region of the human enterovirus (HEV) genome has become the reference test for typing field isolates. This study describes a new strategy for typing HEV at the serotype level that uses a reverse transcription-PCR assay targeting the central part of the VP2 capsid protein. Two pa...

journal_title：Journal of clinical microbiology

authors： Nasri D,Bouslama L,Omar S,Saoudin H,Bourlet T,Aouni M,Pozzetto B,Pillet S

更新日期：2007-08-01 00:00:00

abstract：:Classical microbiological diagnosis of human brucellosis is time-consuming, hazardous, and subject to variable interpretation. Intact-cell matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was evaluated for the routine identification of Brucella spp. Analysis of mass peak patt...

journal_title：Journal of clinical microbiology

authors： Karger A,Melzer F,Timke M,Bettin B,Kostrzewa M,Nöckler K,Hohmann A,Tomaso H,Neubauer H,Al Dahouk S

更新日期：2013-09-01 00:00:00

abstract：:The increasing incidence of invasive aspergillosis, a life-threatening infection in immunocompromised patients, emphasizes the need to improve the diagnostic tools for this disease. We established a LightCycler-based real-time PCR assay to detect and quantify rapidly, specifically, and sensitively Aspergillus fumigatu...

journal_title：Journal of clinical microbiology

authors： Spiess B,Buchheidt D,Baust C,Skladny H,Seifarth W,Zeilfelder U,Leib-Mösch C,Mörz H,Hehlmann R

更新日期：2003-05-01 00:00:00

abstract：:Pseudobacteremias in blood cultures performed on the BACTEC radiometric blood culture system have been reported. We report three cases of cross contamination with Mycobacterium avium that occurred when mycobacteria were cultured with the BACTEC 460 TB system. Malfunction of the needle sterilization heating block was d...

journal_title：Journal of clinical microbiology

authors： Vannier AM,Tarrand JJ,Murray PR

更新日期：1988-09-01 00:00:00

abstract：:The aim of this study was to describe the frequency of positive Aspergillus tests in COVID-19 patients and investigate the association between COVID-19 and a positive Aspergillus test result. We compared the proportion of positive Aspergillus tests in COVID-19 patients admitted to the ICU for > 24 hours with two contr...

journal_title：Journal of clinical microbiology

authors： Yusuf E,Vonk A,van den Akker JPC,Bode L,Sips GJ,Rijnders BJA,de Steenwinkel J,Verkaik NJ,Vogel M,van der Eerden M,van Westreenen M

更新日期：2020-12-04 00:00:00

abstract：:A bacterium with a spiral shape and bipolar, single, sheathed flagella was isolated from the livers of mice with active, chronic hepatitis. The bacteria also colonized the cecal and colonic mucosae of mice. The bacterium grew at 37 degrees C under microaerophilic and anaerobic conditions, rapidly hydrolyzed urea, was ...

journal_title：Journal of clinical microbiology

authors： Fox JG,Dewhirst FE,Tully JG,Paster BJ,Yan L,Taylor NS,Collins MJ Jr,Gorelick PL,Ward JM

更新日期：1994-05-01 00:00:00

abstract：:Collecting whole blood on filter paper simplifies the processing, transport, and storage of specimens used for the diagnosis of human immunodeficiency virus type 1 (HIV-1) and other tests. Specimens may be collected in tropical or rural areas with minimal facilities for handling specimens. To compare simulated tropica...

journal_title：Journal of clinical microbiology

authors： Mitchell C,Jennings C,Brambilla D,Aldrovandi G,Amedee AM,Beck I,Bremer JW,Coombs R,Decker D,Fiscus S,Fitzgibbon J,Luzuriaga K,Moye J,Palumbo P,Reichelderfer P,Somasundaran M,Stevens W,Frenkel L,Dried Blood Spot Workin

更新日期：2008-09-01 00:00:00

abstract：:Malaria-specific rapid diagnostic tests (RDTs) targeting aldolase show highly variable sensitivities. We assessed diversity in Plasmodium falciparum and P. vivax aldolases by sequencing the coding genes from parasites of various origins. The results show that aldolases are highly conserved, indicating that antigenic d...

journal_title：Journal of clinical microbiology

authors： Lee N,Baker J,Bell D,McCarthy J,Cheng Q

更新日期：2006-12-01 00:00:00

abstract：:We report the first case of disseminated infection with both Actinomyces graevenitzii and Mycobacterium tuberculosis and review the medical literature. Concomitant actinomycosis and tuberculosis is very rare. The potential of the facultatively aerobic, newly described A. graevenitzii for disseminated invasive infectio...

journal_title：Journal of clinical microbiology

authors： Tietz A,Aldridge KE,Figueroa JE

更新日期：2005-06-01 00:00:00

abstract：:To diagnose catheter-related sepsis without removing the catheter, we evaluated the differential positivity times of cultures of blood drawn simultaneously from central venous catheter and peripheral sites. In a 450-bed cancer reference center, simultaneous central- and peripheral-blood cultures were prospectively per...

journal_title：Journal of clinical microbiology

authors： Blot F,Schmidt E,Nitenberg G,Tancrède C,Leclercq B,Laplanche A,Andremont A

更新日期：1998-01-01 00:00:00

abstract：:Hybridomas producing monoclonal antibodies to VP7, a major neutralizing protein of serotype 9 rotavirus (strain W161), were prepared. One monoclonal antibody, W161-6A1, was shown to neutralize only serotype 9 rotavirus strains and reacted specifically with serotype 9 rotaviruses in an enzyme-linked immunosorbent assay...

journal_title：Journal of clinical microbiology

authors： Midthun K,Valdesuso J,Kapikian AZ,Hoshino Y,Green KY

更新日期：1989-09-01 00:00:00

abstract：:In five separate fecal collections spanning three years, group A rotaviruses were detected by enzyme-linked immunosorbent assay in 35 (25%) of 142 specimens obtained from nondiarrheic, hospitalized neonates in Blantyre, Malawi. Molecular characterization of each strain identified, for the first time in neonates, a sho...

journal_title：Journal of clinical microbiology

authors： Cunliffe NA,Rogerson S,Dove W,Thindwa BD,Greensill J,Kirkwood CD,Broadhead RL,Hart CA

更新日期：2002-04-01 00:00:00

abstract：:Patients with infection from bacteremic Bartonella spp., tested using Bartonella Alphaproteobacteria growth medium (BAPGM), were retrospectively categorized into one of two groups that included those whose blood was collected once (group 1; n = 55) or three times (group 2; n = 36) within a 1-week period. Overall, 19 p...

journal_title：Journal of clinical microbiology

authors： Pultorak EL,Maggi RG,Mascarelli PE,Breitschwerdt EB

更新日期：2013-06-01 00:00:00

abstract：:When Shiga toxin-producing Escherichia coli (STEC) strains emerged as agents of human disease, two types of toxin were identified: Shiga toxin type 1 (Stx1) (almost identical to Shiga toxin produced by Shigella dysenteriae type 1) and the immunologically distinct type 2 (Stx2). Subsequently, numerous STEC strains have...

journal_title：Journal of clinical microbiology

authors： Scheutz F,Teel LD,Beutin L,Piérard D,Buvens G,Karch H,Mellmann A,Caprioli A,Tozzoli R,Morabito S,Strockbine NA,Melton-Celsa AR,Sanchez M,Persson S,O'Brien AD

更新日期：2012-09-01 00:00:00

abstract：:This study evaluated the performance of the Rapidec Carba NP test, which was introduced recently into the market for the detection of carbapenemase production in a broad spectrum of β-lactamase-producing Enterobacteriaceae clinical isolates. In total, 252 clinical Enterobacteriaceae isolates that had been genetically ...

journal_title：Journal of clinical microbiology

authors： Hombach M,von Gunten B,Castelberg C,Bloemberg GV

更新日期：2015-12-01 00:00:00

abstract：:Widespread use of conjugate pneumococcal polysaccharide-protein vaccines may alter the spectrum of pneumococci producing invasive disease. Novel sensitive diagnostic methods would be valuable for monitoring the epidemiology of pneumococcal disease within populations and vaccine recipients. Ideally, these methods shoul...

journal_title：Journal of clinical microbiology

authors： Leeming JP,Cartwright K,Morris R,Martin SA,Smith MD,South-West Pneumococcus Study Group.

更新日期：2005-10-01 00:00:00

abstract：:Clostridium difficile is a major cause of antibiotic-associated gastrointestinal illness. Recently, an increased incidence of hospital-acquired infections with severe outcomes has been reported in North America and Europe. Current molecular-typing methods for detection of outbreaks and nosocomial transmission are labo...

journal_title：Journal of clinical microbiology

authors： Marsh JW,O'Leary MM,Shutt KA,Pasculle AW,Johnson S,Gerding DN,Muto CA,Harrison LH

更新日期：2006-07-01 00:00:00

abstract：:Human adenovirus of strains subgenus F (AdV F) are the most common strains detected in acute gastroenteritis cases in developing countries. Subgenus F is represented by AdV serotype 40 (AdV-40) and AdV-41. Most of the reports have described the predominance of AdV-41 in acute gastroenteritis cases. To gain insight int...

journal_title：Journal of clinical microbiology

authors： Dey RS,Ghosh S,Chawla-Sarkar M,Panchalingam S,Nataro JP,Sur D,Manna B,Ramamurthy T

更新日期：2011-02-01 00:00:00

abstract：:The relationship of coagulase-negative staphylococcal slime production and adherence with the development and outcome of bloodstream infections in two Finnish hospitals was evaluated. Analysis of 64 strains from 62 adult septicemias disclosed 34 (53%) adherent slime producers. In comparison, only 142 (29%) of 489 sing...

journal_title：Journal of clinical microbiology

authors： Kotilainen P

更新日期：1990-12-01 00:00:00

abstract：:Clostridium difficile is the leading cause of antibiotic-associated diarrhea and pseudomembranous colitis, which have significant morbidity and mortality. Accurate and timely diagnosis is critical. Repeat enzyme immunoassay testing for C. difficile toxin has been recommended because of <100% sensitivity. All C. diffic...

journal_title：Journal of clinical microbiology

authors： Cardona DM,Rand KH

更新日期：2008-11-01 00:00:00