Protein characterization of Australian spotted fever group rickettsiae and monoclonal antibody typing of Rickettsia honei.

abstract：:This report describes a DNA amplification procedure for routine identification of heat-labile-toxin-producing Escherichia coli. Two oligonucleotide primers were used in a polymerase chain reaction procedure to amplify a highly conserved region of the A subunit of the heat-labile enterotoxin gene. Amplifications were d...

journal_title：Journal of clinical microbiology

authors： Victor T,du Toit R,van Zyl J,Bester AJ,van Helden PD

更新日期：1991-01-01 00:00:00

abstract：:We report a case of cerebral phaeohyphomycosis in a 36-year-old male caused by the neurotropic fungus Ramichloridium obovoideum (Matushima) de Hoog 1977 (Ramichloridium mackenziei Campbell et Al-Hedaithy 1993). This man resided in the Middle East, where the fungus appears to be endemic and, possibly, geographically re...

journal_title：Journal of clinical microbiology

authors： Sutton DA,Slifkin M,Yakulis R,Rinaldi MG

更新日期：1998-03-01 00:00:00

abstract：:A comparison of three different commercial media was made to assess their recovery of anaerobic organisms from the blood stream. The three media used were the 50-ml brain heart infusion broth with added CO2 (Pfizer), the 50-ml Thiol broth with added CO2 (Difco), and the 50-ml prereduced, supplemented peptone broth in ...

journal_title：Journal of clinical microbiology

authors： Mangels JI,Lindberg LH,Vosti KL

更新日期：1977-05-01 00:00:00

abstract：:Legionella is the causative agent for Legionnaires' disease (LD) and is responsible for several large outbreaks in the world. More than 90% of LD cases are caused by Legionella pneumophila, and studies on the origin and transmission routes of this pathogen rely on adequate molecular characterization of isolates. Curre...

journal_title：Journal of clinical microbiology

authors： Bosch T,Euser SM,Landman F,Bruin JP,IJzerman EP,den Boer JW,Schouls LM

更新日期：2015-10-01 00:00:00

abstract：:Enzyme-linked immunosorbent assays were developed for the quantitation of anti-reovirus immunoglobulin G (IgG), IgA, and IgM in serum. The assays were specific, sensitive, and reproducible and measured antibodies to the reovirus group antigen. They should permit diagnosis of recent reovirus infection and longitudinal ...

journal_title：Journal of clinical microbiology

authors： Richardson SC,Bishop RF,Smith AL

更新日期：1988-09-01 00:00:00

abstract：:Genetic diversity among Borrelia burgdorferi isolates recovered from the skin of Lyme disease patients was assessed by ribosomal DNA (rDNA) spacer restriction fragment length polymorphism analysis, genomic restriction site polymorphism analysis, and plasmid content analysis. There was a significant association between...

journal_title：Journal of clinical microbiology

authors： Iyer R,Liveris D,Adams A,Nowakowski J,McKenna D,Bittker S,Cooper D,Wormser GP,Schwartz I

更新日期：2001-08-01 00:00:00

abstract：:To improve the detection of Mycoplasma pulmonis contamination of isolates of cilia-associated respiratory (CAR) bacillus, we developed a nested PCR method using primers for 16S rRNA gene sequences. Of 140 samples of 16 different CAR bacillus isolates, 73 (52%) were inhibitory in the first PCR, as indicated by the abse...

journal_title：Journal of clinical microbiology

authors： Schoeb TR,Dybvig K,Keisling KF,Davidson MK,Davis JK

更新日期：1997-07-01 00:00:00

abstract：:Astrovirus infections were detected by enzyme immunoassay in 12 (5%) of 251 stool samples from children with gastroenteritis from Bogota, Colombia. In addition, astroviruses were detected by reverse transcription-PCR in 3 (10%) of 29 stool samples negative for other enteric pathogens collected in Caracas, Venezuela, f...

journal_title：Journal of clinical microbiology

authors： Medina SM,Gutierrez MF,Liprandi F,Ludert JE

更新日期：2000-09-01 00:00:00

abstract：:Two colorimetric methods that use Alamar Blue or 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) for assaying the in vitro activities of antifungal agents have been described. We report that both tests performed similarly when the antifungal activity of amphotericin B against Candida albicans wa...

journal_title：Journal of clinical microbiology

authors： Jahn B,Stüben A,Bhakdi S

更新日期：1996-08-01 00:00:00

abstract：:The automated and computerized AutoMicrobic system (AMS; Vitek Systems, Inc., subsidiary of McDonnell Douglas, Hazelwood, Mo.) was evaluated as a means of identifying the Enterobacteriaceae. The Micro-ID system (General Diagnostics, Morris Plains, N.J.) and, when necessary, conventional tubed media were used for compa...

journal_title：Journal of clinical microbiology

authors： Burdash NM,Teti G,West ME,Bannister ER,Manos JP

更新日期：1981-02-01 00:00:00

abstract：:We assessed neutralizing antibody titers to adenovirus serotype 5 (Ad5) and six rare adenovirus serotypes, serotypes 11, 35, 50, 26, 48, and 49, in pediatric populations in sub-Saharan Africa. We observed a clear age dependence of Ad5-specific neutralizing antibody titers. These data will help to guide the development...

journal_title：Journal of clinical microbiology

authors： Thorner AR,Vogels R,Kaspers J,Weverling GJ,Holterman L,Lemckert AA,Dilraj A,McNally LM,Jeena PM,Jepsen S,Abbink P,Nanda A,Swanson PE,Bates AT,O'Brien KL,Havenga MJ,Goudsmit J,Barouch DH

更新日期：2006-10-01 00:00:00

abstract：:Microsporidia (phylum Microsproa) have recently become recognized as common opportunistic protozoans in the United States and worldwide, particularly affecting immunodeficient patients. Microsporidian organisms within the genus Encephalitozoon are the cause of nephrologic, ophthalmic, pneumologic, gastroenteric, and s...

journal_title：Journal of clinical microbiology

authors： Enriquez FJ,Ditrich O,Palting JD,Smith K

更新日期：1997-03-01 00:00:00

abstract：:A group of 300 clinically derived isolates of coagulase-negative staphylococci were tested in parallel with the API STAPH-IDENT system (Analytab Products) and 14 conventional biochemical tests contained in Kloos and Schleifer's simplified scheme for identification of human Staphylococcus species. STAPH-IDENT is a mini...

journal_title：Journal of clinical microbiology

authors： Almeida RJ,Jorgensen JH

更新日期：1983-08-01 00:00:00

abstract：:The relationship of coagulase-negative staphylococcal slime production and adherence with the development and outcome of bloodstream infections in two Finnish hospitals was evaluated. Analysis of 64 strains from 62 adult septicemias disclosed 34 (53%) adherent slime producers. In comparison, only 142 (29%) of 489 sing...

journal_title：Journal of clinical microbiology

authors： Kotilainen P

更新日期：1990-12-01 00:00:00

abstract：:Salmonella enterica subsp. enterica serovar Agona was not frequently encountered in Finland until an increase in rates of isolation among animal and feed was seen in 1994. A small outbreak among cattle farms in the regions of Oulu and Vaasa in northwestern Finland in 1994-1995 included eight farms. After the outbreak,...

journal_title：Journal of clinical microbiology

authors： Lindqvist N,Siitonen A,Pelkonen S

更新日期：2002-10-01 00:00:00

abstract：:A mathematical analysis of serological data has provided the basis for a new serotyping routine. This paper describes the process whereby the results of agglutination reactions between the standard O antigens of Escherichia coli and rabbit antisera raised against them were used to produce a much simpler, faster, and c...

journal_title：Journal of clinical microbiology

authors： Thompson CJ

更新日期：1987-05-01 00:00:00

abstract：:Viral load testing for cytomegalovirus (CMV) has become the standard for the diagnosis of infection and monitoring of therapy at many transplant centers. However, no viral load test has been approved by the FDA. Therefore, many laboratories rely on laboratory-developed assays. This study evaluated the performance char...

journal_title：Journal of clinical microbiology

authors： Caliendo AM,Ingersoll J,Fox-Canale AM,Pargman S,Bythwood T,Hayden MK,Bremer JW,Lurain NS

更新日期：2007-06-01 00:00:00

abstract：:Six isolates of Mycobacterium avium of genotype dnaJ(+) IS901(-) IS1311(+) IS1245(+) and serotypes 6 (n = 1), 6/9, (n = 2), and 9 (n = 3) were obtained within a 5-month period from a human immunodeficiency virus-negative patient treated for tuberculosis. The isolates were identified with PvuII restriction fragment len...

journal_title：Journal of clinical microbiology

authors： Dvorska L,Bartos M,Ostadal O,Kaustova J,Matlova L,Pavlik I

更新日期：2002-10-01 00:00:00

abstract：:Lactococcus garvieae is a Gram-positive coccus that has morphological and biochemical similarities to enterococci. L. garvieae strains rare human pathogens, with only a few cases reported in the literature, mainly as a cause of infective endocarditis. L. garvieae is well known as a fish pathogen, and in some of the re...

journal_title：Journal of clinical microbiology

authors： Navas ME,Hall G,El Bejjani D

更新日期：2013-06-01 00:00:00

abstract：:A modified oxidase reagent, 1% tetramethyl-p-phenylenediamine in dimethyl sulfoxide, proved superior to the routinely used 1% aqueous tetramethyl-p-phenylenediamine dihydrochloride in detecting weakly oxidase-positive gram-negative bacteria after 24 h of growth on agar media (40 of 40 positive versus 22 of 40 positive...

journal_title：Journal of clinical microbiology

authors： Tarrand JJ,Gröschel DH

更新日期：1982-10-01 00:00:00

abstract：:Multidrug-resistant opportunistic pathogens have become endemic to the veterinary hospital environment. Escherichia coli isolates resistant to 12 antibiotics were isolated from two dogs that were housed in the intensive care unit at The University of Georgia Veterinary Teaching Hospital within 48 h of each other. Revi...

journal_title：Journal of clinical microbiology

authors： Sanchez S,McCrackin Stevenson MA,Hudson CR,Maier M,Buffington T,Dam Q,Maurer JJ

更新日期：2002-10-01 00:00:00

abstract：:Sensitivity of the enzyme-linked immunosorbent assay for detecting serum antibodies to rotavirus was greatly enhanced when rotavirus particles were fragmented by chaotropic agents (NaSCN or guanidine hydrochloride) before adsorption of the antigens to the solid phase. For detecting fecal antibodies, the addition of fe...

journal_title：Journal of clinical microbiology

authors： Inouye S,Matsuno S,Yamaguchi H

更新日期：1984-02-01 00:00:00

abstract：:A DNA macroarray containing 465 intragenic amplicons was designed to identify Staphylococcus aureus at the species level and to type S. aureus isolates. The genes selected included those encoding (i) S. aureus-specific proteins, (ii) staphylococcal and enterococcal proteins mediating antibiotic resistance and factors ...

journal_title：Journal of clinical microbiology

authors： Trad S,Allignet J,Frangeul L,Davi M,Vergassola M,Couve E,Morvan A,Kechrid A,Buchrieser C,Glaser P,El-Solh N

更新日期：2004-05-01 00:00:00

abstract：:An enzyme immunoassay (EIA) for serum immunoglobulin M (IgM) antibodies to rubella virus based on enzyme labeling of viral antigen was developed. The sensitivity of the EIA for the detection of recent rubella virus infection was evaluated by using 115 rubella-IgM-antibody-positive serum specimens, which were confirmed...

journal_title：Journal of clinical microbiology

authors： Seppänen H

更新日期：1990-04-01 00:00:00

abstract：:The need to evaluate antiviral treatment response and the emergence of resistance have made the human immunodeficiency virus (HIV) viral load assay a major feature of the diagnostic monitoring of HIV-infected individuals. The objective of this study was to evaluate the utility of the recently In Vitro Diagnostic Medic...

journal_title：Journal of clinical microbiology

authors： Oliver AR,Pereira SF,Clark DA

更新日期：2007-11-01 00:00:00

abstract：:Among 234 isolates comprising 26 different Candida species colonizing the oropharynx of 181 (54.3% of 399 surveyed) HIV-infected outpatients, 27 (11.7%) were fluconazole resistant. Antibacterial treatment was associated with increased rates of yeast colonization, while antiretroviral therapy and pneumococcal vaccinati...

journal_title：Journal of clinical microbiology

authors： Yang YL,Hung CC,Wang AH,Tseng FC,Leaw SN,Tseng YT,Su CL,Chen HT,Lauderdale TL,Lo HJ

更新日期：2010-07-01 00:00:00

abstract：:In an evaluation of the API 20NE for the identification of Burkholderia spp., 792/800 (99%) Burkholderia pseudomallei and 17/19 (89%) B. cepacia isolates were correctly identified but 10 B. mallei and 98 B. thailandensis isolates were not correctly identified. A latex agglutination test was positive for 796/800 (99.5%...

journal_title：Journal of clinical microbiology

authors： Amornchai P,Chierakul W,Wuthiekanun V,Mahakhunkijcharoen Y,Phetsouvanh R,Currie BJ,Newton PN,van Vinh Chau N,Wongratanacheewin S,Day NP,Peacock SJ

更新日期：2007-11-01 00:00:00

abstract：:A novel PCR detection assay that amplifies the Helicobacter pylori-specific vacuolating cytotoxin gene (vacA) and thus enables rapid diagnosis of infection is described. Additionally, a real-time probe hybridization melting point analysis assay to detect all three mutations in the 23S rRNA gene associated with clarith...

journal_title：Journal of clinical microbiology

authors： Chisholm SA,Owen RJ,Teare EL,Saverymuttu S

更新日期：2001-04-01 00:00:00

abstract：:Recovery of Mycobacterium tuberculosis from sputa treated with cetyl-pyridinium chloride (CPC) and stored for 20 +/- 9 days was significantly higher than that from sputa that were untreated and processed by the N-acetyl-L-cisteine-NaOH method. Addition of CPC is useful for isolation of M. tuberculosis from sputa subje...

journal_title：Journal of clinical microbiology

authors： Pardini M,Varaine F,Iona E,Arzumanian E,Checchi F,Oggioni MR,Orefici G,Fattorini L

更新日期：2005-01-01 00:00:00

abstract：:We describe a multiplex allele-specific (MAS)-PCR assay to detect simultaneously mutations in the first and third bases of the embB gene codon 306ATG. These mutations are known to confer ethambutol (EMB) resistance in the majority of clinical Mycobacterium tuberculosis isolates worldwide. The mutated bases are reveale...

journal_title：Journal of clinical microbiology

authors： Mokrousov I,Narvskaya O,Limeschenko E,Otten T,Vyshnevskiy B

更新日期：2002-05-01 00:00:00