Enzyme-linked immunosorbent assays for measurement of reovirus immunoglobulin G, A, and M levels in serum.


:Enzyme-linked immunosorbent assays were developed for the quantitation of anti-reovirus immunoglobulin G (IgG), IgA, and IgM in serum. The assays were specific, sensitive, and reproducible and measured antibodies to the reovirus group antigen. They should permit diagnosis of recent reovirus infection and longitudinal study of the development of reovirus group antibodies.


J Clin Microbiol


Richardson SC,Bishop RF,Smith AL




Has Abstract


1988-09-01 00:00:00












  • Microbiological assessment of 24- and 48-h changes and management of semiclosed circuits from ventilators in a neonatal intensive care unit.

    abstract::The contamination of semiclosed disposable circuits of Healthdyne and Bourns ventilators was studied in a newborn intensive care unit over a 2-year period. A total of 379 fluid samples was obtained from inspiratory and expiratory tubing condensates and traps and from thermal humidifier columns fed with prefilled conta...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Malecka-Griggs B

    更新日期:1986-02-01 00:00:00

  • Complement fixation antibody test for human nocardiosis.

    abstract::Complement-fixing antibody was detected in 13 of 16 patients with histological and/or culture evidence of infection with Nocardia species. The antigen used was a filtrate of soluble substances secreted into liquid growth medium by cultures of N. asteroides. Apparent false positives reactions were found in three of thr...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Shainhouse JZ,Pier AC,Stevens DA

    更新日期:1978-11-01 00:00:00

  • Prevalence of rotavirus, adenovirus, norovirus, and astrovirus infections and coinfections among hospitalized children in northern France.

    abstract::From January to December 2007, 973 stool specimens were prospectively collected from children hospitalized for gastroenteritis signs or from neonates and premature cases who were born in two French hospital settings in the north of France. They were tested by rapid enzyme immunoassay (EIA) analyses for rotavirus and a...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Tran A,Talmud D,Lejeune B,Jovenin N,Renois F,Payan C,Leveque N,Andreoletti L

    更新日期:2010-05-01 00:00:00

  • Antigenic characteristics of Borrelia burgdorferi isolates from ixodid ticks in California.

    abstract::Twenty (1.4%) of 1,421 adult Ixodes pacificus ticks and 2 (20%) of 10 adult Ixodes neotomae ticks collected in five counties of northern California were found to contain spirochetes by direct immunofluorescence examination of their tissues with a polyvalent conjugate. Borreliae isolated from the tissues of nine of the...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Lane RS,Pascocello JA

    更新日期:1989-10-01 00:00:00

  • Seroepidemiological evidence of avian influenza A virus transmission to pigs in southern China.

    abstract::Recently, three novel avian-origin swine influenza viruses (SIVs) were first isolated from pigs in Guangdong Province, southern China, yet little is known about the seroprevalence of avian influenza viruses among pigs in southern China. Here, we report for the first time the seroprevalence of avian H3, H4, and H6 infl...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Su S,Qi W,Chen J,Zhu W,Huang Z,Xie J,Zhang G

    更新日期:2013-02-01 00:00:00

  • Detection of Epstein-Barr virus DNA in cerebrospinal fluid for diagnosis of AIDS-related central nervous system lymphoma.

    abstract::The diagnostic utility of Epstein-Barr virus (EBV) DNA detection in cerebrospinal fluid for the diagnosis of central nervous system lymphoma was evaluated with two different PCR assays to test a collection of cerebrospinal fluid samples from 24 AIDS patients with central nervous system disorders. A PCR assay amplifyin...

    journal_title:Journal of clinical microbiology

    pub_type: 临床试验,杂志文章


    authors: Arribas JR,Clifford DB,Fichtenbaum CJ,Roberts RL,Powderly WG,Storch GA

    更新日期:1995-06-01 00:00:00

  • Genetic diversity of intimin genes of attaching and effacing Escherichia coli strains.

    abstract::In this study, we determined the sequences of four intimin variant genes detected in attaching and effacing Escherichia coli isolates of human origin. Three of them were novel and were designated eae-eta (eta), eae-iota (iota), and eae-kappa (kappa). The fourth was identical to the recently described eae-zeta (zeta), ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Zhang WL,Köhler B,Oswald E,Beutin L,Karch H,Morabito S,Caprioli A,Suerbaum S,Schmidt H

    更新日期:2002-12-01 00:00:00

  • Asymptomatic respiratory tract infection with Chlamydia pneumoniae TWAR.

    abstract::Chlamydia pneumoniae is a newly recognized organism associated with respiratory tract infections. Asymptomatic infection with C. pneumoniae, although it has been suggested to occur, has not been previously documented. We describe two asymptomatic individuals infected with this organism; these infections demonstrate th...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Hyman CL,Augenbraun MH,Roblin PM,Schachter J,Hammerschlag MR

    更新日期:1991-09-01 00:00:00

  • When Should Asymptomatic Persons Be Tested for COVID-19?

    abstract::On 24 August 2020, the Centers for Disease Control and Prevention (CDC) updated its website to highlight that asymptomatic individuals, even those with exposure to a COVID-19-positive contact, do not necessarily need to be tested unless they have medical conditions associated with increased risk of severe illness from...

    journal_title:Journal of clinical microbiology

    pub_type: 社论


    authors: Schuetz AN,Hemarajata P,Mehta N,Campbell S,Mitchell S,Palavecino E,Butler-Wu S,Miller MB

    更新日期:2020-12-17 00:00:00

  • Cervical and vaginal flora specimens are highly concordant with respect to bacterial vaginosis-associated organisms and commensal Lactobacillus species in women of reproductive age.

    abstract::Matched vaginal and cervical specimens from 96 subjects were analyzed by quantitative PCR for the presence and concentration of bacterial vaginosis-associated microbes and commensal Lactobacillus spp. Detection of these microbes was 92% concordant, indicating that microbial floras at these body sites are generally sim...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Smith WL,Hedges SR,Mordechai E,Adelson ME,Trama JP,Gygax SE,Kaunitz AM,Hilbert DW

    更新日期:2014-08-01 00:00:00

  • Cobas-Bact system for identification of members of the family Enterobacteriaceae in 4 h 20 min.

    abstract::The Cobas-Bact (Roche Diagnostics, Basel, Switzerland) new rotor for the identification (ID) in 4 h 20 min of 33 members of the family Enterobacteriaceae to genus and species level was evaluated by testing 444 strains of which 398 belonged to common species and 46 belonged to rare species of Enterobacteriaceae. Each s...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Wenger A,Tissières LJ,Praplan FR,Kamm WR,Bille J

    更新日期:1987-01-01 00:00:00

  • Diagnosis of disseminated candidiasis by measurement of urine D-arabinitol/L-arabinitol ratio.

    abstract::Relative amounts of D-arabinitol (fungal origin) and L-arabinitol (part of normal human metabolism) in urine were determined by gas chromatography and mass spectrometry from 61 hospitalized patients with hematological malignancies. Seventeen neutropenic patients with acute leukemia (with 53 samples) had disseminated y...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Lehtonen L,Anttila VJ,Ruutu T,Salonen J,Nikoskelainen J,Eerola E,Ruutu P

    更新日期:1996-09-01 00:00:00

  • Enzyme immunoassay for identification of heat-killed mycobacteria belonging to the Mycobacterium tuberculosis and Mycobacterium avium complexes and derived from early cultures.

    abstract::A simple enzyme-linked immunosorbent assay was developed for the identification of cultured mycobacteria belonging to the Mycobacterium tuberculosis complex, the Mycobacterium avium complex, and Mycobacterium kansasii. Six monoclonal antibodies were used: two (F23-49 and F24-2) were specific for the M. tuberculosis co...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Schöningh R,Verstijnen CP,Kuijper S,Kolk AH

    更新日期:1990-04-01 00:00:00

  • Emergence of a clinical daptomycin-resistant Staphylococcus aureus isolate during treatment of methicillin-resistant Staphylococcus aureus bacteremia and osteomyelitis.

    abstract::The emergence of a clinically daptomycin-resistant Staphylococcus aureus isolate occurred during treatment of methicillin-resistant S. aureus bacteremia and probable vertebral osteomyelitis. The breakthrough isolate was indistinguishable from pretreatment daptomycin-susceptible isolates by pulsed-field gel electrophor...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Marty FM,Yeh WW,Wennersten CB,Venkataraman L,Albano E,Alyea EP,Gold HS,Baden LR,Pillai SK

    更新日期:2006-02-01 00:00:00

  • Comparison of the semisolid agar antifungal susceptibility test with the NCCLS M38-P broth microdilution test for screening of filamentous fungi.

    abstract::Antifungal susceptibility testing of pathogenic molds is being developed. A simple screening semisolid agar antifungal susceptibility (SAAS) test accurately measures susceptibilities of yeasts. The performance of the SAAS screening test for filamentous fungi was assessed by comparing MICs of four antifungals (amphoter...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Kuzucu C,Rapino B,McDermott L,Hadley S

    更新日期:2004-03-01 00:00:00

  • Molecular Assay for Detection of Ciprofloxacin Resistance in Neisseria gonorrhoeae Isolates from Cultures and Clinical Nucleic Acid Amplification Test Specimens.

    abstract::We developed a real-time PCR assay to detect single nucleotide polymorphisms associated with ciprofloxacin resistance in specimens submitted for nucleic acid amplification testing (NAAT). All three single nucleotide polymorphism (SNP) targets produced high sensitivity and specificity values. The presence of ≥2 SNPs wa...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Peterson SW,Martin I,Demczuk W,Bharat A,Hoang L,Wylie J,Allen V,Lefebvre B,Tyrrell G,Horsman G,Haldane D,Garceau R,Wong T,Mulvey MR

    更新日期:2015-11-01 00:00:00

  • Clinical evaluation of multiple inflammation biomarkers for diagnosis and prognosis for patients with systemic inflammatory response syndrome.

    abstract::A multiplexed biomarker bundle consisting of nine different inflammation markers was evaluated regarding their diagnostic and prognostic performances in 159 adult systemic inflammatory response syndrome (SIRS) patients enrolled at the emergency department. Fibronectin, interleukin-8 (IL-8), biotin, and neutrophil gela...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Reichsoellner M,Raggam RB,Wagner J,Krause R,Hoenigl M

    更新日期:2014-11-01 00:00:00

  • Complete genome analysis of coxsackievirus A2, A4, A5, and A10 strains isolated from hand, foot, and mouth disease patients in China revealing frequent recombination of human enterovirus A.

    abstract::Coxsackievirus (CV) strains CVA2, CVA4, CVA5, and CVA10 were isolated from patients with hand, foot, and mouth disease during a 2009 outbreak in China. Full genome sequences for four representative strains, CVA2/SD/CHN/09 (A2SD09), CVA4/SZ/CHN/09 (A4SZ09), CVA5/SD/CHN/09 (A5SD09), and CVA10/SD/CHN/09 (A10SD09), were d...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Hu YF,Yang F,Du J,Dong J,Zhang T,Wu ZQ,Xue Y,Jin Q

    更新日期:2011-07-01 00:00:00

  • The Borrelia burgdorferi 37-kilodalton immunoblot band (P37) used in serodiagnosis of early lyme disease is the flaA gene product.

    abstract::The 37-kDa protein (P37) of Borrelia burgdorferi is an antigen that elicits an early immunoglobulin M (IgM) antibody response in Lyme disease patients. The P37 gene was cloned from a B. burgdorferi genomic library by screening with antibody from a Lyme disease patient who had developed a prominent humoral response to ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Gilmore RD Jr,Murphree RL,James AM,Sullivan SA,Johnson BJ

    更新日期:1999-03-01 00:00:00

  • Outbreak of Candida parapsilosis endophthalmitis after cataract extraction and intraocular lens implantation.

    abstract::Between November 1983 and January 1984, 13 cases of Candida parapsilosis endophthalmitis occurred in Florida, Georgia, and Tennessee in patients who had had an intraocular lens implantation (IOLI) or cataract extraction with an IOLI. This outbreak followed the introduction in July 1983 of a new brand of balanced salt ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: McCray E,Rampell N,Solomon SL,Bond WW,Martone WJ,O'Day D

    更新日期:1986-10-01 00:00:00

  • Rapid detection of rpoB gene mutations in rifampin-resistant Mycobacterium tuberculosis isolates in shanghai by using the amplification refractory mutation system.

    abstract::Rapid detection of drug resistance in Mycobacterium tuberculosis is essential for efficient treatment and control of this pathogen. The amplification refractory mutation system (ARMS) was used to detect mutations in the rifampin resistance-determining region of the rpoB gene. A total of 39 rifampin-resistant M. tuberc...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Fan XY,Hu ZY,Xu FH,Yan ZQ,Guo SQ,Li ZM

    更新日期:2003-03-01 00:00:00

  • Multicenter evaluation of a commercial PCR-enzyme-linked immunosorbent assay diagnostic kit (Onychodiag) for diagnosis of dermatophytic onychomycosis.

    abstract::We prospectively evaluated a new PCR-enzyme-linked immunosorbent assay kit (Onychodiag; BioAdvance, France) for the diagnosis of dermatophytic onychomycosis by testing nail samples from 438 patients with suspected onychomycosis and from 108 healthy controls in three independent laboratories. In two laboratories, sampl...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,多中心研究


    authors: Savin C,Huck S,Rolland C,Benderdouche M,Faure O,Noacco G,Menotti J,Candolfi E,Pelloux H,Grillot R,Coupe S,Derouin F

    更新日期:2007-04-01 00:00:00

  • Clonal groups of Salmonella typhimurium in New York State.

    abstract::The epidemiology of 278 strains of Salmonella typhimurium isolated from 1973 to 1981 from animals in New York State was studied by using four "fingerprinting" techniques, bacteriophage type (B.R. Callow, J. Hyg. 57:346-359, 1959), biotype (J. P. Duguid, E. S. Anderson, G. A. Alfredsson, R. Barker, and D. C. Old, J. Me...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: McDonough PL,Timoney JF,Jacobson RH,Khakhria R

    更新日期:1989-04-01 00:00:00

  • Specificity, sensitivity, and reproducibility among the fluorescent treponemal antibody-absorption test, the microhemagglutination assay for Treponema pallidum antibodies, and the hemagglutination treponemal test for syphilis.

    abstract::Using 920 sera, we compared the specificity and reproducibility of the hemagglutination treponemal test for syphilis with those of the fluorescent treponemal antibody-absorption test and the microhemagglutination assay for Treponema pallidum antibodies; we found all three tests to be comparable. However, the hemagglut...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Larsen SA,Hambie EA,Pettit DE,Perryman MW,Kraus SJ

    更新日期:1981-10-01 00:00:00

  • Characteristics and biotypes of Pasteurella multocida isolated from humans.

    abstract::Fifty-two isolates of Pasteurella (48 strains of Pasteurella multocida and 4 strains of atypical Pasteurella) were identified by conventional and commercial test systems. All strains fermented glucose, sucrose, and fructose in purple broth base (Difco Laboratories) with bromocresol purple as indicator, although the at...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Oberhofer TR

    更新日期:1981-03-01 00:00:00

  • Accuracy of Chlamydia trachomatis antigen detection methods in a low-prevalence population in a primary care setting.

    abstract::We compared a direct fluorescent-antibody stain (DFA) and an enzyme immunoassay (EIA) with a standard cell culture technique for the detection of Chlamydia trachomatis infection in women in an urban family practice setting. We also evaluated a DFA sample in a commercial laboratory to determine the interlaboratory reli...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Gann PH,Herrmann JE,Candib L,Hudson RW

    更新日期:1990-07-01 00:00:00

  • Bartonella henselae and Bartonella elizabethae as potential canine pathogens.

    abstract::Bartonella henselae or Bartonella elizabethae DNA from EDTA-anticoagulated blood samples obtained from four dogs was amplified and sequenced. The results showed that B. elizabethae should be added to the list of Bartonella species (i.e., B. vinsonii subsp. berkhoffii, B. henselae, and B. clarridgeiae) that are current...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Mexas AM,Hancock SI,Breitschwerdt EB

    更新日期:2002-12-01 00:00:00

  • Development and application of real-time PCR assay for quantification of Mycobacterium ulcerans DNA.

    abstract::Buruli ulcer, an infection caused by Mycobacterium ulcerans, is, after tuberculosis and leprosy, the third most common mycobacterial disease. The mode of transmission of M. ulcerans is not exactly known, but since Buruli ulcer often occurs in focalized swampy areas, it is assumed that there is a reservoir of the patho...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Rondini S,Mensah-Quainoo E,Troll H,Bodmer T,Pluschke G

    更新日期:2003-09-01 00:00:00

  • Molecular epidemiology of oral treponemes in patients with periodontitis and in periodontitis-resistant subjects.

    abstract::The etiologic role of oral treponemes in human periodontitis is still under debate. Although seen by dark-field microscopy in large numbers, their possible role is still unclear since they comprise some 60 different phylotypes, most of which are still uncultured. To determine their status as mere commensals or opportu...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Moter A,Riep B,Haban V,Heuner K,Siebert G,Berning M,Wyss C,Ehmke B,Flemmig TF,Göbel UB

    更新日期:2006-09-01 00:00:00

  • Evaluation of a novel PCR-based assay for detection and identification of Chlamydia trachomatis serovars in cervical specimens.

    abstract::The aims of this study were to compare a novel PCR-based Chlamydia trachomatis detection and genotyping (Ct-DT) assay with the FDA-approved, commercially available C. trachomatis detection Hybrid Capture 2 (HC2) assay and to investigate the C. trachomatis serovar distribution among young women in a rural Costa Rican s...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Quint K,Porras C,Safaeian M,González P,Hildesheim A,Quint W,van Doorn LJ,Silva S,Melchers W,Schiffman M,Rodríguez AC,Wacholder S,Freer E,Cortes B,Herrero R,Costa Rican Vaccine Trial Group.

    更新日期:2007-12-01 00:00:00