Cetyl-pyridinium chloride is useful for isolation of Mycobacterium tuberculosis from sputa subjected to long-term storage.

abstract：:Human papillomavirus (HPV) is causally associated with anal cancer, as HPV DNA is detected in up to 90% of anal intraepithelial neoplasias and anal cancers. With the gradual increase of anal cancer rates, there is a growing need to establish reliable and clinically relevant methods to detect anal cancer precursors. In...

journal_title：Journal of clinical microbiology

authors： Low HC,Silver MI,Brown BJ,Leng CY,Blas MM,Gravitt PE,Woo YL

更新日期：2015-02-01 00:00:00

abstract：:A group of 254 isolates of coagulase-negative staphylococci were tested in parallel for novobiocin susceptibility by using P agar and Mueller-Hinton agar. Zones of inhibition of 16 mm or less around a 5-micrograms novobiocin disk on Mueller-Hinton agar indicated novobiocin resistance, as demonstrated by Staphylococcus...

journal_title：Journal of clinical microbiology

authors： Almeida RJ,Jorgensen JH

更新日期：1982-12-01 00:00:00

abstract：:Both Staphylococcus epidermidis and Staphylococcus aureus are important causes of infections associated with catheters and other medical devices. It has recently been shown that not only S. epidermidis but also S. aureus can produce slime and carries the ica operon responsible for slime production. In the operon, coex...

journal_title：Journal of clinical microbiology

authors： Arciola CR,Baldassarri L,Montanaro L

更新日期：2001-06-01 00:00:00

abstract：:Buruli ulcer, an infection caused by Mycobacterium ulcerans, is, after tuberculosis and leprosy, the third most common mycobacterial disease. The mode of transmission of M. ulcerans is not exactly known, but since Buruli ulcer often occurs in focalized swampy areas, it is assumed that there is a reservoir of the patho...

journal_title：Journal of clinical microbiology

authors： Rondini S,Mensah-Quainoo E,Troll H,Bodmer T,Pluschke G

更新日期：2003-09-01 00:00:00

abstract：:We describe the first case of Epstein-Barr virus (EBV)-associated thymic carcinoid tumor found by in situ hybridization (ISH) on paraffin-embedded sections. ISH revealed that both tumor cells and infiltrated lymphocytes were EBV positive, while a few EBV-infected lymphocytes were detected in 2 of 11 thymuses and 1 of ...

journal_title：Journal of clinical microbiology

authors： Takeuchi H,Fujita H,Iwasaki F,Takeuchi T,Imadome K,Okumiya T,Matsui T,Takahashi M

更新日期：2004-06-01 00:00:00

abstract：:Hepatitis C virus (HCV) infection typically leads to antibody response within weeks after primary infection. Here, we describe the case of a child infected with HCV by mother-to-child transmission who remained persistently seronegative despite the presence of high levels of circulating HCV RNA. ...

journal_title：Journal of clinical microbiology

authors： Larouche A,Gaëtan G,El-Bilali N,Quesnel-Vallières M,Martin SR,Alvarez F,Shoukry NH,Soudeyns H

更新日期：2012-07-01 00:00:00

abstract：:The binding of human immunoglobulin G by Fc receptors in herpes simplex virus (HSV)-infected cells can cause false-positive interpretations in the immunofluorescence test for HSV antibody. When the infected cell smears were treated with 10% glacial acetic acid for 5 min and rinsed in phosphate-buffered saline before t...

journal_title：Journal of clinical microbiology

authors： Gallo D

更新日期：1986-10-01 00:00:00

abstract：:Transfusion-transmissible infections among 808 blood donors in Ghana were investigated in 1999. Antibody seroprevalences of 3.8, 0.7, 8.4, and 13.5%, respectively, for human immunodeficiency virus, human T-cell lymphotrophic virus type 1, hepatitis C virus (HCV), and Treponema pallidum were obtained. The seroprevalenc...

journal_title：Journal of clinical microbiology

authors： Ampofo W,Nii-Trebi N,Ansah J,Abe K,Naito H,Aidoo S,Nuvor V,Brandful J,Yamamoto N,Ofori-Adjei D,Ishikawa K

更新日期：2002-09-01 00:00:00

abstract：:Human papillomavirus type 16 (HPV 16) plays a cardinal role in the pathogenesis of cervical cancer. HPV 16 has intratypic variants which show different geographical distributions and different oncogenic potentials. To analyze the presence of sequence variations of HPV 16 variants in northeast China, 71 cervical carcin...

journal_title：Journal of clinical microbiology

authors： Shang Q,Wang Y,Fang Y,Wei L,Chen S,Sun Y,Li B,Zhang F,Gu H

更新日期：2011-07-01 00:00:00

abstract：:Glutaraldehyde-fixed sheep erythrocytes (GA-SRBC) were used in the indirect hemagglutination test for the detection of Pasteurella antibody. GA-SRBC were stable for at least 6 months. Heat extract or potassium thiocyanate extract antigens of Pasteurella strains could be adsorbed onto GA-SRBC or tanned GA-SRBC, respect...

journal_title：Journal of clinical microbiology

authors： Sawada T,Rimler RB,Rhoades KR

更新日期：1982-05-01 00:00:00

abstract：:The sequences of mink astroviruses collected from 11 farms in Denmark and Sweden were analyzed and found to be homologous with one another but different from those of other astroviruses. A species-specific reverse transcriptase-PCR for mink astrovirus was established and shown to be suitable for the analysis of clinic...

journal_title：Journal of clinical microbiology

authors： Mittelholzer C,Englund L,Hedlund KO,Dietz HH,Svensson L

更新日期：2003-11-01 00:00:00

abstract：:Group G Streptococcus has been implicated as a causative agent of pharyngitis in outbreak situations, but its role in endemic disease remains elusive. We found an unexpected inverse association of Streptococcus dysgalactiae subsp. equisimilis colonization and sore throat in a study of 2,194 children of 3 to 15 years o...

journal_title：Journal of clinical microbiology

authors： Tartof SY,Farrimond F,de Matos JA,Reis JN,Ramos RT,Andrade AN,dos Reis MG,Riley LW

更新日期：2011-01-01 00:00:00

abstract：:The applicability of whole-cell hybridization for the identification of pathogenic bacteria in blood from septic patients was examined. Oligonucleotide probes, fluorescently labeled with fluorescein isothiocyanate, directed against the variable regions of the 16S rRNAs of the following bacterial species and/or genera ...

journal_title：Journal of clinical microbiology

authors： Jansen GJ,Mooibroek M,Idema J,Harmsen HJ,Welling GW,Degener JE

更新日期：2000-02-01 00:00:00

abstract：:Classical microbiological diagnosis of human brucellosis is time-consuming, hazardous, and subject to variable interpretation. Intact-cell matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was evaluated for the routine identification of Brucella spp. Analysis of mass peak patt...

journal_title：Journal of clinical microbiology

authors： Karger A,Melzer F,Timke M,Bettin B,Kostrzewa M,Nöckler K,Hohmann A,Tomaso H,Neubauer H,Al Dahouk S

更新日期：2013-09-01 00:00:00

abstract：:HIV-1 non-B subtypes/circulating recombinant forms (CRFs) are increasing worldwide. Since subtype identification can be clinically relevant, we assessed the added value in HIV-1 subtyping using updated molecular phylogeny (Mphy) and the performance of routinely used automated tools. Updated Mphy (2015 updated referenc...

journal_title：Journal of clinical microbiology

authors： Fabeni L,Berno G,Fokam J,Bertoli A,Alteri C,Gori C,Forbici F,Takou D,Vergori A,Zaccarelli M,Maffongelli G,Borghi V,Latini A,Pennica A,Mastroianni CM,Montella F,Mussini C,Andreoni M,Antinori A,Perno CF,Santoro MM,

更新日期：2017-09-01 00:00:00

abstract：:Pyridine-2-azo-p-dimethylanaline cephalosporin (PADAC), a chromogenic reagent which is purple and changes to yellow upon cleavage of its beta-lactam ring, was evaluated in comparison with other chromogenic cephalosporins. PADAC exhibited little antimicrobial activity against gram-negative bacteria, but did have good a...

journal_title：Journal of clinical microbiology

authors： Jones RN,Wilson HW,Novick WJ Jr

更新日期：1982-04-01 00:00:00

abstract：:We studied the plasmid profiles of 136 Shigella isolates in Bangladesh to determine whether plasmid profiles could be used for differentiation of strains for epidemiological studies. Many different plasmid patterns were observed within each species, indicating that many genetically different strains of Shigella are re...

journal_title：Journal of clinical microbiology

authors： Tacket CO,Shahid N,Huq MI,Alim AR,Cohen ML

更新日期：1984-08-01 00:00:00

abstract：:A DNA microarray for detection of Campylobacter spp. was recently developed and applied to detect Campylobacter spp. directly from chicken feces. Sixty-five pooled chicken cloacal swab samples from 650 individual broiler chickens were included in the study. The results of Campylobacter sp. detection obtained with DNA ...

journal_title：Journal of clinical microbiology

authors： Keramas G,Bang DD,Lund M,Madsen M,Bunkenborg H,Telleman P,Christensen CB

更新日期：2004-09-01 00:00:00

abstract：:A duplex real-time SYBR Green LightCycler PCR (LC-PCR) assay with DNA extraction using the QIAamp DNA Stool Mini kit was evaluated with regard to detection of 8 of 17 species of food- or waterborne pathogens in five stool specimens in 2 h or less. The protocol used the same LC-PCR with 20 pairs of specific primers. Th...

journal_title：Journal of clinical microbiology

authors： Fukushima H,Tsunomori Y,Seki R

更新日期：2003-11-01 00:00:00

abstract：:Characterization of 102 Haemophilus strains by the Minitek differentiation system (BBL Microbiology Systems, Cockeysville, Md.) was compared with that by the conventional method. The results obtained by the two methods were in good agreement (P less than 0.001; test of independence). Therefore, this system was found t...

journal_title：Journal of clinical microbiology

authors： Kawakami Y,Okimura Y,Kanai M

更新日期：1981-11-01 00:00:00

abstract：:A passive hemagglutination (PHA) assay for antibody to Pseudomonas aeruginosa exotoxin is described which utilizes chromic chloride-treated ovine erythrocytes coated with purified toxin. PHA antitoxin titers correlated well with those obtained by a cytotoxicity neutralization assay (r = 0.91, P less than 0.001), where...

journal_title：Journal of clinical microbiology

authors： Pollack M,Taylor NS

更新日期：1977-07-01 00:00:00

abstract：:A monoclonal antibody (MAb), designated CHA 437, was developed against herpes simplex virus (HSV). This MAb (isotype, immunoglobulin G2b K) reacted with HSV type 1 and HSV type 2. It showed no cross-reactivity with varicella-zoster virus, cytomegalovirus, or Epstein-Barr virus. Direct detection of HSV antigen in clini...

journal_title：Journal of clinical microbiology

authors： Pouletty P,Chomel JJ,Thouvenot D,Catalan F,Rabillon V,Kadouche J

更新日期：1987-05-01 00:00:00

abstract：:From 1974 to 1994, 2,033 specimens from children were submitted for cultures for anaerobic bacteria. Eighty-three Veillonella spp. were recovered from 83 children (4%). Most Veillonella species were recovered from abscesses, aspiration pneumonias, burns, bites, and sinuses. The infections were polymicrobial in 79 (95%...

journal_title：Journal of clinical microbiology

authors： Brook I

更新日期：1996-05-01 00:00:00

abstract：:We developed a sensitive and specific sodB-based quantitative PCR assay to detect Ehrlichia spp. The assay's limit of detection was 5 copies/reaction, and it did not amplify nonspecific DNA. Compared with a 16S rRNA gene PCR target, the sodB target may offer an improved molecular diagnostic assay to detect Ehrlichia s...

journal_title：Journal of clinical microbiology

authors： Qurollo BA,Riggins D,Comyn A,Zewde MT,Breitschwerdt EB

更新日期：2014-11-01 00:00:00

abstract：:Over a decade ago the first cryptococcal lateral flow assay (LFA) became available. This affordable point-of-care test has undeniably led to tremendous improvements in early detection of cryptococcosis (1,2). However, from several studies it must be concluded that the reliability of recently introduced LFA's falls sho...

journal_title：Journal of clinical microbiology

authors： Shi D,Haas PJ,Boekhout T,Hahn RC,Hagen F

更新日期：2021-01-20 00:00:00

abstract：:Human adenovirus type 4 is one of the major serotypes isolated from patients with adenoviral conjunctivitis. In 2001 we encountered nosocomial infections with epidemic conjunctivitis in the ophthalmology ward of one hospital in Sapporo, which is in the northern part of Japan. Adenoviruses were isolated from the patien...

journal_title：Journal of clinical microbiology

authors： Ariga T,Shimada Y,Ohgami K,Tagawa Y,Ishiko H,Aoki K,Ohno S

更新日期：2004-08-01 00:00:00

abstract：:Strains of Mycobacterium avium, Mycobacterium intracellulare, Mycobacterium scrofulaceum, Mycobacterium xenopi, and Mycobacterium gordonae were identified by high-performance liquid chromatography (HPLC) analysis of mycolic acids as bromophenacyl esters. HPLC criteria were used to develop a flow chart identification s...

journal_title：Journal of clinical microbiology

authors： Butler WR,Thibert L,Kilburn JO

更新日期：1992-10-01 00:00:00

abstract：:This is the first report in Israel of a cluster of cases of diarrhea associated with the isolation of Escherichia coli O157 in stool samples. E. coli O157:NM (nonmotile), producing verotoxins 1 and 2 was isolated from the stool samples of three infants less than 1 year of age with diarrhea and from a fourth asymptomat...

journal_title：Journal of clinical microbiology

authors： Lerman Y,Cohen D,Gluck A,Ohad E,Sechter I

更新日期：1992-02-01 00:00:00

abstract：:This study investigated a rare area of endemicity with a high prevalence of both hepatitis B virus (HBV) and hepatitis C virus (HCV) infections, in Putian County, China. Among 1,050 subjects, the overall prevalence of HBsAg seropositivity was 15.8%, and that of anti-HCV seropositivity was 28.9%. Intrafamilial viral tr...

journal_title：Journal of clinical microbiology

authors： Lu WP,Lin GX,Shi S,Dong JH

更新日期：2012-06-01 00:00:00

abstract：:Opisthorchis viverrini is an important food-borne trematode in Southeast Asia. The infection causes significant morbidity in terms of hepatobiliary diseases and cholangiocarcinoma. The aim of this study was to improve the sensitivity of the PCR-based diagnosis of O. viverrini infection. A new fecal DNA extraction prot...

journal_title：Journal of clinical microbiology

authors： Duenngai K,Sithithaworn P,Rudrappa UK,Iddya K,Laha T,Stensvold CR,Strandgaard H,Johansen MV

更新日期：2008-01-01 00:00:00