Human papillomavirus type 16 variant analysis of E6, E7, and L1 [corrected] genes and long control region in [corrected] cervical carcinomas in patients in northeast China.

abstract：:Vibrio cholerae serotype O1 organisms that do not produce cholera toxin and, in fact, lack the genetic material encoding the enterotoxin have recently been detected in coastal regions of the United States. Although these organisms have been assumed to be nonpathogenic, they have been considered a potential reservoir o...

journal_title：Journal of clinical microbiology

authors： Johnston JM,McFarland LM,Bradford HB,Caraway CT

更新日期：1983-05-01 00:00:00

abstract：:The current article summarizes recent changes in nomenclature for fungi of medical importance published in the years 2018 to 2019, including new species and revised names for existing ones. Many of the revised names have been widely adopted without further discussion. However, those that concern common pathogens of hu...

journal_title：Journal of clinical microbiology

authors： Borman AM,Johnson EM

更新日期：2021-01-21 00:00:00

abstract：:We report a novel IS711 chromosomal location that is specific for the Brucella genotype ST27 previously associated with Pacific marine mammals and human zoonotic infection in New Zealand and Peru. Our data support the previous observation that this peculiar genotype is distinct from those commonly isolated from the At...

journal_title：Journal of clinical microbiology

authors： Cloeckaert A,Bernardet N,Koylass MS,Whatmore AM,Zygmunt MS

更新日期：2011-11-01 00:00:00

abstract：:For testing bacterial susceptibility to PD131628, a 5-micrograms disk and the following tentative interpretive criteria may be used: > or = 19 mm for susceptible (MIC, < or = 1.0 micrograms/ml), 16 to 18 mm for intermediate (MIC, 2.0 micrograms/ml), and < or = 15 mm for resistant (MIC, > or = 4.0 micrograms/ml). For s...

journal_title：Journal of clinical microbiology

authors： Barry AL,Fuchs PC,Allen SD,Tenover FC,Jorgensen JH,Reller LB

更新日期：1995-01-01 00:00:00

abstract：:A rapid and sensitive PCR assay for the detection of Candida albicans DNA in serum was established. DNA from human serum samples was purified using the QIAamp blood kit, which proved to be a fast and simple method for isolating minute amounts of Candida DNA from clinical specimens for diagnosis of invasive candidiasis...

journal_title：Journal of clinical microbiology

authors： Wahyuningsih R,Freisleben HJ,Sonntag HG,Schnitzler P

更新日期：2000-08-01 00:00:00

abstract：:Nasopharyngeal swabs (NPSs) are being widely used as specimens for multiplex real-time reverse transcription (RT)-PCR for respiratory virus detection. However, it remains unclear whether NPS specimens are optimal for all viruses targeted by multiplex RT-PCR. In addition, the procedure to obtain NPS specimens causes co...

journal_title：Journal of clinical microbiology

authors： Kim YG,Yun SG,Kim MY,Park K,Cho CH,Yoon SY,Nam MH,Lee CK,Cho YJ,Lim CS

更新日期：2016-12-28 00:00:00

abstract：:Nocardia isolations increased from 0.7 to 11.7/1,000 acid-fast bacillus and mycological cultures (P less than 0.000001). Only three isolations from one patient represented infection. Pseudoepidemic strain identity was confirmed by DNA fingerprinting; the isolate causing infection was distinct. The end of the pseudoepi...

journal_title：Journal of clinical microbiology

authors： Patterson JE,Chapin-Robertson K,Waycott S,Farrel P,McGeer A,McNeil MM,Edberg SC

更新日期：1992-05-01 00:00:00

abstract：:The continuous spread of community-acquired methicillin-resistant Staphylococcus aureus (caMRSA) and the introduction of these highly virulent isolates into hospitals represent increasing threats. The timely recognition of caMRSA strains is crucial for infection control purposes. Thus, we developed a PCR-based assay f...

journal_title：Journal of clinical microbiology

authors： Strommenger B,Braulke C,Pasemann B,Schmidt C,Witte W

更新日期：2008-02-01 00:00:00

abstract：:A protocol for multilocus sequence typing (MLST) of methicillin-resistant Staphylococcus aureus (MRSA) was adapted to real-time LightCycler System PCR for efficient and rapid amplification of seven housekeeping genes in the same PCR run and real-time detection of the products. The method was evaluated on a representat...

journal_title：Journal of clinical microbiology

authors： Berglund C,Mölling P,Sjöberg L,Söderquist B

更新日期：2005-09-01 00:00:00

abstract：:An evaluation was undertaken to determine the optimal method for testing the susceptibilities of 100 clinical isolates and two reference strains of Enterococcus spp. to vancomycin in vitro. Six testing methods were studied by using the following media and incubation times: agar screen with the Synergy Quad Plate (Reme...

journal_title：Journal of clinical microbiology

authors： Kohner PC,Patel R,Uhl JR,Garin KM,Hopkins MK,Wegener LT,Cockerill FR 3rd

更新日期：1997-12-01 00:00:00

abstract：:Helicobacter pylori virulence determinants have not previously been studied in detail in Latin Americans with H. pylori infections. We characterized the vacA (vacuolating cytotoxin gene A) and cagA (cytotoxin-associated gene A) types of more than 400 single-colony isolates from 20 patients in Mexico City. For 17 patie...

journal_title：Journal of clinical microbiology

authors： Morales-Espinosa R,Castillo-Rojas G,Gonzalez-Valencia G,Ponce de León S,Cravioto A,Atherton JC,López-Vidal Y

更新日期：1999-09-01 00:00:00

abstract：:Buruli ulcer, an infection caused by Mycobacterium ulcerans, is, after tuberculosis and leprosy, the third most common mycobacterial disease. The mode of transmission of M. ulcerans is not exactly known, but since Buruli ulcer often occurs in focalized swampy areas, it is assumed that there is a reservoir of the patho...

journal_title：Journal of clinical microbiology

authors： Rondini S,Mensah-Quainoo E,Troll H,Bodmer T,Pluschke G

更新日期：2003-09-01 00:00:00

abstract：:We report a case of cerebral phaeohyphomycosis in a 36-year-old male caused by the neurotropic fungus Ramichloridium obovoideum (Matushima) de Hoog 1977 (Ramichloridium mackenziei Campbell et Al-Hedaithy 1993). This man resided in the Middle East, where the fungus appears to be endemic and, possibly, geographically re...

journal_title：Journal of clinical microbiology

authors： Sutton DA,Slifkin M,Yakulis R,Rinaldi MG

更新日期：1998-03-01 00:00:00

abstract：:We retrospectively compared the utility of a fungal isolation device (Isolator) versus conventional techniques for recovering fungal organisms from blood cultures obtained from neutropenic cancer patients. Positive cultures were deemed true pathogens, possible pathogens, or contaminants according to laboratory and cli...

journal_title：Journal of clinical microbiology

authors： Creger RJ,Weeman KE,Jacobs MR,Morrissey A,Parker P,Fox RM,Lazarus HM

更新日期：1998-01-01 00:00:00

abstract：:Random amplified polymorphic DNA (RAPD) was used to better characterize the genotypic relatedness among medically important Candida species. By using short oligomer primers (10-mers) with arbitrarily chosen sequences in the polymerase chain reaction, distinctive and reproducible sets of polymerase chain reaction produ...

journal_title：Journal of clinical microbiology

authors： Lehmann PF,Lin D,Lasker BA

更新日期：1992-12-01 00:00:00

abstract：:The use of monoclonal antibody (MAb) p63-27, which is reactive with the major immediate-early human cytomegalovirus (HCMV) protein pp72, was explored for the rapid diagnosis of HCMV viruria. The rapid assay detected all but 1 of 19 specimens identified by standard virus isolation methods from 1,676 newborn urine speci...

journal_title：Journal of clinical microbiology

authors： Boppana SB,Smith RJ,Stagno S,Britt WJ

更新日期：1992-03-01 00:00:00

abstract：:We designed a highly sensitive and specific polymerase chain reaction assay for the detection of Epstein-Barr virus (EBV)-related sequences in B-cell DNA of EBV-seropositive healthy individuals. By using this assay, we were able to amplify at least 10 copies of a plasmid containing the BamHI-W region, which is repeate...

journal_title：Journal of clinical microbiology

authors： Wagner HJ,Bein G,Bitsch A,Kirchner H

更新日期：1992-11-01 00:00:00

abstract：:Nocardia cyriacigeorgica is a recently characterized species within the genus of Nocardia. We report a brain abscess, following a primary pulmonary colonization, due to this species in a human immunodeficiency virus-infected patient. This case confirms that isolation of Nocardia in sputum is associated with a high ris...

journal_title：Journal of clinical microbiology

authors： Barnaud G,Deschamps C,Manceron V,Mortier E,Laurent F,Bert F,Boiron P,Vinceneux P,Branger C

更新日期：2005-09-01 00:00:00

abstract：:A simple method, using commercial disks to detect Escherichia coli mutator strains, is proposed. The breakpoint for detecting strains with a mutation frequency >/=5 x 10(-7) was established at >/=70 and >/=20 colonies in the inhibition zone of fosfomycin and rifampin disks, respectively, after seeding 100 microl of an...

journal_title：Journal of clinical microbiology

authors： Galán JC,Tato M,Baquero MR,Turrientes C,Baquero F,Martinez JL

更新日期：2004-09-01 00:00:00

abstract：:Imferon, an iron-dextran complex, enhances the growth of Neisseria gonorrhoeae and N. meningitidis. The use of Imferon as a replacement for ferric nitrate, in a defined supplement for GC agar significantly increased the average colony sizes of both gonococci and meningococci. In comparison with Thayer-Martin medium, I...

journal_title：Journal of clinical microbiology

authors： Payne SM,Finkelstein RA

更新日期：1977-09-01 00:00:00

abstract：:Indirect hemagglutinating and immunofluorescent antibody responses to Herpesvirus hominis types 1 and 2 were compared to neutralizing antibody responses in infected humans from whom H. hominis type 1 or 2 was isolated. The indirect immunofluorescent antibody test was shown to be the most sensitive and specific for pri...

journal_title：Journal of clinical microbiology

authors： Johnson LD,Fuccillo DA,Stalder H,Oxman MA,Fraser CE,Madden DL

更新日期：1979-03-01 00:00:00

abstract：:A microhematocrit concentration method (MH) for immediate diagnosis of Chagas' disease during the acute stage or in congenital cases was standardized. Parasitemia as low as 1,000 parasites per ml was detected, after centrifugation of six 50-microliters capillary tubes, by 10-min microscopic observation of each buffy c...

journal_title：Journal of clinical microbiology

authors： Feilij H,Muller L,Gonzalez Cappa SM

更新日期：1983-08-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of measles immunoglobulin G antibody (MEASELISA). This assay was found to be comparable to the measles hemagglutination inhibition (HAI) test. Approximately 500 sera from three centers were tested by MEASELISA and the HAI test. MEASELISA demo...

journal_title：Journal of clinical microbiology

authors： Boteler WL,Luipersbeck PM,Fuccillo DA,O'Beirne AJ

更新日期：1983-05-01 00:00:00

abstract：:The AutoMicrobic system (AMS; Vitek Systems, Inc., Hazelwood, Mo.) was studied for its ability to produce accurate and precise MIC interpretations for Pseudomonas aeruginosa susceptibility to gentamicin, tobramycin, and amikacin. MICs were determined in parallel on 200 selected P. aeruginosa isolates by using the AMS ...

journal_title：Journal of clinical microbiology

authors： Woolfrey BF,Lally RT,Ederer MN,Quall CO

更新日期：1984-04-01 00:00:00

abstract：:Anthopsis deltoidea was found to be the cause of an olecranon bursitis in a 79-year-old golfer. Serial histological sections of the olecranon bursa showed faintly stained, brown-walled, septate, hyphal elements in the centers of the necrotic debris. The combination of bursectomy and flucytosine treatment cured the inf...

journal_title：Journal of clinical microbiology

authors： Kwon-Chung KJ,Droller DD

更新日期：1984-08-01 00:00:00

abstract：:Hepatitis E virus (HEV) is a major cause of hepatitis. We evaluated five HEV antibody diagnostic assays by using outbreak specimens. The Abbott immunoglobulin G (IgG), Genelabs IgG, and Walter Reed Army Institute of Research (WRAIR) IgM assays were about 90% sensitive; the Abbott IgG and WRAIR total Ig and IgM assays ...

journal_title：Journal of clinical microbiology

authors： Myint KS,Endy TP,Gibbons RV,Laras K,Mammen MP Jr,Sedyaningsih ER,Seriwatana J,Glass JS,Narupiti S,Corwin AL

更新日期：2006-04-01 00:00:00

abstract：:A serotyping scheme for Vibrio vulnificus predicated on the detection of lipopolysaccharide (LPS) antigens is proposed. The serovar O typing scheme used to type V. vulnificus employs polyclonal antisera raised in rabbits immunized with heat-killed whole-cell vaccines. Polyclonal typing sera produced in this manner cro...

journal_title：Journal of clinical microbiology

authors： Martin SJ,Siebeling RJ

更新日期：1991-08-01 00:00:00

abstract：:Carbohydrate antigen detection, nucleic acid probe detection, and bacterial culture are commonly used to confirm group A streptococcus (GAS) pharyngitis. Compared to standard throat swab specimens, the sensitivities of these tests with mouth specimens are poor. When testing for GAS pharyngitis, the throat remains the ...

journal_title：Journal of clinical microbiology

authors： Fox JW,Marcon MJ,Bonsu BK

更新日期：2006-07-01 00:00:00

abstract：:In Mycobacterium smegmatis and a limited number of Mycobacterium tuberculosis strains, the involvement of alterations of the 16S rRNA gene (rrs) in resistance to kanamycin has been shown. To investigate the extent to which mutations in a specific region of the rrs gene and the kanamycin-resistant phenotype in clinical...

journal_title：Journal of clinical microbiology

authors： Suzuki Y,Katsukawa C,Tamaru A,Abe C,Makino M,Mizuguchi Y,Taniguchi H

更新日期：1998-05-01 00:00:00

abstract：:Sapporo-like viruses (SLVs) are associated with acute gastroenteritis in humans. Due to a limited supply of available reagents for diagnosis, little is known about the incidence and pathogenicity of these viruses. We have developed a first-generation generic reverse transcriptase (RT) PCR assay based on a single prime...

journal_title：Journal of clinical microbiology

authors： Vinjé J,Deijl H,van der Heide R,Lewis D,Hedlund KO,Svensson L,Koopmans MP

更新日期：2000-02-01 00:00:00