Pseudoepidemic of Nocardia asteroides associated with a mycobacterial culture system.

abstract：:Random urine samples from hospitalized patients (n = 550) and seeded sterile filtered urine samples (n = 730) were used to test a membrane filtration technique, Qualture (Future Medical Technologies International, Inc., West Palm Beach, Fla.), for the detection and identification of uropathogens. Results for each samp...

journal_title：Journal of clinical microbiology

authors： Friedman MP,Danielski JM,Day TE,Dunne JC,Evangelista AT,Freeman TR

更新日期：1991-11-01 00:00:00

abstract：:We developed a liquid bead microarray (LBMA) assay for genotyping genital human papillomaviruses (HPVs) based on the MY09-MY11-HMB01 PCR system and the reverse line blot (RLB) assay probe sequences. Using individual HPV plasmids, we were able to detect as few as 50 copies per reaction. In two separate retrospective st...

journal_title：Journal of clinical microbiology

authors： Feng Q,Cherne S,Winer RL,Balasubramanian A,Lee SK,Hawes SE,Kiviat NB,Koutsky LA

更新日期：2009-03-01 00:00:00

abstract：:A total of 1,835 Yersinia spp. were isolated from 925 (60.5%) of 1,530 wild mice and from 139 (79.9%) of 174 moles living in mountainous areas of eastern Shimane Prefecture, Japan. The Yersinia spp. included 1,106 Yersinia enterocolitica, 26 Y. enterocolitica-like, 176 Yersinia mollaretii, 149 Yersinia frederiksenii, ...

journal_title：Journal of clinical microbiology

authors： Fukushima H,Gomyoda M,Kaneko S

更新日期：1990-11-01 00:00:00

abstract：:A biphasic blood culture bottle (BiPB: GIBCO Laboratories, North Andover, Mass.) with an architectural design that physically separates the agar slant from the broth was compared with a conventional vented monophasic bottle (MPB-A) for use in the routine culture of blood. Both bottles contained tryptic soy broth. Tryp...

journal_title：Journal of clinical microbiology

authors： Weckbach LS,Staneck JL

更新日期：1986-04-01 00:00:00

abstract：:We sought evidence of toxoplasma parasitemia among 37 people with active or dormant Toxoplasma gondii infection or no evidence of infection. DNA was extracted from erythrocyte-free portions of blood samples, and the T. gondii B1 gene was amplified by the polymerase chain reaction. Evidence of T. gondii parasitemia was...

journal_title：Journal of clinical microbiology

authors： Filice GA,Hitt JA,Mitchell CD,Blackstad M,Sorensen SW

更新日期：1993-09-01 00:00:00

abstract：:Clostridioides (Clostridium) difficile infection (CDI) is the most common causative pathogen of health care-associated gastrointestinal infections; however, due to the overlap of clinical symptoms with those of other causes of acute gastroenteritis, the selection of the most appropriate laboratory test is difficult. F...

journal_title：Journal of clinical microbiology

authors： Krutova M,Briksi A,Tkadlec J,Zajac M,Matejkova J,Nyc O,Drevinek P

更新日期：2019-09-24 00:00:00

abstract：:A combined method of the Limulus amebocyte lysate (LAL) test and the enzyme-linked immunosorbent assay for quantitation of endotoxin was developed based on our observation that the antigenicity of coagulogen, a major protein in LAL, was lost when LAL reacted with endotoxin as shown by immunoblotting. Determination of ...

journal_title：Journal of clinical microbiology

authors： Zhang GH,Baek L,Koch C

更新日期：1988-08-01 00:00:00

abstract：:A study to compare the polymerase chain reaction (PCR) test with the cell culture method in diagnosing urogenital Chlamydia trachomatis infections was performed. From 497 patients (212 women, 285 men) attending an outpatient clinic for sexually transmitted diseases, a total of 814 samples (female patients, cervix and ...

journal_title：Journal of clinical microbiology

authors： Vogels WH,van Voorst Vader PC,Schröder FP

更新日期：1993-05-01 00:00:00

abstract：:Recent reports of natural paratuberculosis (or Johne's disease) in rabbits, foxes, and stoats has focused debate on the presence and importance of wildlife reservoirs in the epidemiology of this disease. This paper describes an extensive study investigating 18 nonruminant wildlife species for evidence of paratuberculo...

journal_title：Journal of clinical microbiology

authors： Beard PM,Daniels MJ,Henderson D,Pirie A,Rudge K,Buxton D,Rhind S,Greig A,Hutchings MR,McKendrick I,Stevenson K,Sharp JM

更新日期：2001-04-01 00:00:00

abstract：:A screening assay for phospholipase C using a chromogenic substrate incorporated into agar medium is described. The assay directly visualizes phospholipase C activity of mycoplasma lysates and membranes on agar plates, or the activity may be measured by spectrophotometry. The results from the assay confirm the presenc...

journal_title：Journal of clinical microbiology

authors： De Silva NS,Quinn PA

更新日期：1987-04-01 00:00:00

abstract：:Candida auris is an emerging worldwide fungal pathogen. Over the past 20 years, 61 patient isolates of C. auris (4 blood and 57 ear) have been obtained from 13 hospitals in Korea. Here, we reanalyzed those molecularly identified isolates using two matrix-assisted laser desorption ionization-time of flight mass spectro...

journal_title：Journal of clinical microbiology

authors： Kwon YJ,Shin JH,Byun SA,Choi MJ,Won EJ,Lee D,Lee SY,Chun S,Lee JH,Choi HJ,Kee SJ,Kim SH,Shin MG

更新日期：2019-03-28 00:00:00

abstract：:An evaluation was undertaken to determine the optimal method for testing the susceptibilities of 100 clinical isolates and two reference strains of Enterococcus spp. to vancomycin in vitro. Six testing methods were studied by using the following media and incubation times: agar screen with the Synergy Quad Plate (Reme...

journal_title：Journal of clinical microbiology

authors： Kohner PC,Patel R,Uhl JR,Garin KM,Hopkins MK,Wegener LT,Cockerill FR 3rd

更新日期：1997-12-01 00:00:00

abstract：:Routine anaerobic subcultures of macroscopically negative blood culture bottles, performed within 1 day of receipt of the culture and after 5 days of incubation, were evaluated. Anaerobes were recovered from 207 (12.3%) of the total 1,688 positive cultures and, of these, 154 were only detected macroscopically, 11 only...

journal_title：Journal of clinical microbiology

authors： Murray PR,Sondag JE

更新日期：1978-10-01 00:00:00

abstract：:Global HIV treatment programs need sensitive and affordable tests to monitor HIV drug resistance. We compared mutant detection by the oligonucleotide ligation assay (OLA), an economical and simple test, to massively parallel sequencing. Nonnucleoside reverse transcriptase inhibitor (K103N, V106M, Y181C, and G190A) and...

journal_title：Journal of clinical microbiology

authors： Beck IA,Deng W,Payant R,Hall R,Bumgarner RE,Mullins JI,Frenkel LM

更新日期：2014-07-01 00:00:00

abstract：:Genetic relationships within ureaplasma serovars were investigated by pulsed-field gel electrophoresis (PFGE). One hundred thirteen Ureaplasma parvum isolates and 78 Ureaplasma urealyticum isolates were different from their ATCC serovar type strains and different within the same serovars. The organisms were geographic...

journal_title：Journal of clinical microbiology

authors： Xiao L,Crabb DM,Moser SA,Duffy LB,Glass JI,Paralanov V,Waites KB

更新日期：2011-09-01 00:00:00

abstract：:A chemically defined medium that simulates female genital tract secretions was developed for the growth of the vaginal microflora. Qualitative and quantitative studies of the growth of predominant components of the vaginal microflora indicated that all vaginal isolates tested were able to grow in this defined medium. ...

journal_title：Journal of clinical microbiology

authors： Geshnizgani AM,Onderdonk AB

更新日期：1992-05-01 00:00:00

abstract：:Salmonella enteritidis, serotype Horsham, identified rarely worldwide, was isolated from the stools of six American Indians with gastroenteritis. The patients were from separate tribes located in three geographically distinct villages. Although significant contact was demonstrated between ill individuals within two tr...

journal_title：Journal of clinical microbiology

authors： Castle SP,Nims LJ,Lapham SC

更新日期：1983-07-01 00:00:00

abstract：:Adolescent children were surveyed for colonization with Mycoplasma hominis and Ureaplasma urealyticum by culturing urine specimens. Rates were compared between three study groups: (i) 397 children attending parochial schools, (ii) 293 children attending an adolescent clinic specializing in adjustment problems, and (ii...

journal_title：Journal of clinical microbiology

authors： Foy H,Kenny G,Bor E,Hammar S,Hickman R

更新日期：1975-09-01 00:00:00

abstract：:The diagnosis of Clostridium difficile infection continues to be a challenge for many clinical microbiology laboratories. A new lateral flow assay, the C.Diff Quik Chek Complete assay, which tests for the presence of both glutamate dehydrogenase (GDH) and C. difficile toxins A and B, was evaluated for its ability to d...

journal_title：Journal of clinical microbiology

authors： Sharp SE,Ruden LO,Pohl JC,Hatcher PA,Jayne LM,Ivie WM

更新日期：2010-06-01 00:00:00

abstract：:Fecal specimens from patients with acute diarrhea were collected from 10 prefectures in Japan over a 6-month period (November 1992 to April 1993), and the specimens that were negative for human group A rotaviruses were screened for the presence of human group C rotaviruses (CHRVs) by the reverse passive hemagglutinati...

journal_title：Journal of clinical microbiology

authors： Kuzuya M,Fujii R,Hamano M,Yamada M,Shinozaki K,Sasagawa A,Hasegawa S,Kawamoto H,Matsumoto K,Kawamoto A,Itagaki A,Funatsumaru S,Urasawa S

更新日期：1998-01-01 00:00:00

abstract：:Analysis of hospital-acquired methicillin-resistant Staphylococcus aureus strains isolated from a tertiary public hospital in Singapore revealed that multisusceptible strains had gradually started to replace the endemic multiresistant strain (ST239-MRSA-III) since 2002. Molecular typing showed that this was a predomin...

journal_title：Journal of clinical microbiology

authors： Hsu LY,Koh TH,Singh K,Kang ML,Kurup A,Tan BH

更新日期：2005-06-01 00:00:00

abstract：:We describe a multiplex reverse transcription-PCR (m-RT-PCR) assay that is able to detect and differentiate all known human parainfluenza viruses (HPIVs). Serial dilution experiments with reference strains that compared cell culture isolation and m-RT-PCR showed sensitivities ranging from 0.0004 50% tissue culture inf...

journal_title：Journal of clinical microbiology

authors： Aguilar JC,Pérez-Breña MP,García ML,Cruz N,Erdman DD,Echevarría JE

更新日期：2000-03-01 00:00:00

abstract：:Species of coagulase-negative staphylococci isolated from urine specimens submitted from both inpatients and outpatients to the clinical microbiology laboratory of a teaching hospital were identified with a biotyping system, with species then correlated by clinical features and antimicrobial susceptibility. Of 145 iso...

journal_title：Journal of clinical microbiology

authors： Nicolle LE,Hoban SA,Harding GK

更新日期：1983-02-01 00:00:00

abstract：:Rabies virus found worldwide and prevalent throughout the United States continues to be a public health concern. Direct-fluorescent antibody (DFA) detection remains the gold standard for rabies virus diagnostics. Assessing the utility of a high-throughput molecular platform such as the QIAsymphony SP/AS, in conjunctio...

journal_title：Journal of clinical microbiology

authors： Dupuis M,Brunt S,Appler K,Davis A,Rudd R

更新日期：2015-09-01 00:00:00

abstract：:Helcococcus kunzii, a nonvirulent member of the human skin flora, has recently been implicated in causing infections in immunosuppressed patients. We report a case of breast abscess associated with H. kunzii in an immunocompetant patient and discuss the criteria used in its identification and our observations of susce...

journal_title：Journal of clinical microbiology

authors： Chagla AH,Borczyk AA,Facklam RR,Lovgren M

更新日期：1998-08-01 00:00:00

abstract：:Bovine tuberculosis (bTB) caused by Mycobacterium bovis is a transmissible disease notifiable to the World Organization for Animal Health and to the European Union, with ongoing efforts of surveillance and eradication in every EU member state. In Germany, a country which has been declared officially free from bovine t...

journal_title：Journal of clinical microbiology

authors： Kohl TA,Utpatel C,Niemann S,Moser I

更新日期：2018-08-27 00:00:00

abstract：:Salmonella enterica serotype Typhi clinical isolates (n = 91) resistant to nalidixic acid (Nal(r)) were collected from sporadic cases and minor outbreaks throughout Vietnam between 1996 and 2004. These isolates were typed and compared by four methods: Vi phage typing, PstI ribotyping, XbaI and SpeI pulsed-field gel el...

journal_title：Journal of clinical microbiology

authors： Le TA,Fabre L,Roumagnac P,Grimont PA,Scavizzi MR,Weill FX

更新日期：2007-11-01 00:00:00

abstract：:The effect of supplementing nutrient substrate with various combinations of concentrations of oleic and linoleic acids on the growth of 11 strains of Staphylococcus aureus was assessed. Whereas increasing the concentration of linoleic acid by itself greatly diminished the growth of all 11 strains, concomitant increase...

journal_title：Journal of clinical microbiology

authors： Campbell IM,Crozier DN,Pawagi AB,Buivids IA

更新日期：1983-08-01 00:00:00

abstract：:DNA restriction analysis was carried out on a sample of 212 epidemiologically unrelated adenovirus (Ad) strains of serotype 7 isolated in Chile, Uruguay, and Argentina from nasopharyngeal aspirates of children with acute lower respiratory disease between 1984 and 1990. The identified genome types were Ad7b (n = 12), A...

journal_title：Journal of clinical microbiology

authors： Kajon A,Wadell G

更新日期：1994-09-01 00:00:00

abstract：:A PCR for protein D (hpd#3) was used to differentiate nontypeable Haemophilus influenzae (NTHI) from Haemophilus haemolyticus. While 90% of nasopharyngeal specimens and 100% of lower-airway specimens from 84 Indigenous Australian children with bronchiectasis had phenotypic NTHI isolates confirmed as H. influenzae, onl...

journal_title：Journal of clinical microbiology

authors： Hare KM,Binks MJ,Grimwood K,Chang AB,Leach AJ,Smith-Vaughan H

更新日期：2012-07-01 00:00:00